scholarly journals Studies on the molecular biological peculiarities of bovine herpesvirus 4

2020 ◽  
Vol 23 (4) ◽  
pp. 411-423
Author(s):  
R. Peshev
Keyword(s):  
Phylogenetic Tree ◽  
Bovine Herpesvirus ◽  
Chain Reaction ◽  
Fragment Analysis ◽  
Biological Features ◽  
Bovine Herpesvirus 4 ◽  
European Strain ◽  
American Strain ◽  
Polymerase Chain ◽  
Herpesvirus 4

Studies on the molecular biological features of bovine herpesvirus 4 (BHV 4) strains isolated in Bulgaria have been conducted. Two types of polymerase chain reaction have been developed and applied to confirm the gB and TK genes. A restrictase fragment analysis was performed using various types of restrictase enzymes. The tested Bulgarian strains differed in their restrictase genomic profile from the reference European strain Movar 33/63 and from the American strain DN 599, and were clearly different each from the other. No clear relationship has been established between the restrictase enzyme profiles and the tropism of the isolated viruses. Sequencing of isolated ВHV 4 strains showed homo­logy with the reference European strain Movar 33/63. After construction of the phylogenetic tree, three ВHV 4 strains were at one branch of the phylogenetic tree, while two other strains were at the branch of reference Movar 33/63 strain. Applied molecular biology methods can be successfully used for differentiation and detailed genetic characterisation of the isolated BHV 4 strains.

scholarly journals Development of a Polymerase Chain Reaction and Restriction Typing Assay for the Diagnosis of Bovine Herpesvirus 1, Bovine Herpesvirus 2, and Bovine Herpesvirus 4 Infections

2002 ◽  
Vol 14 (4) ◽  
pp. 353-356 ◽  
Author(s):  
Luciana De-Giuli ◽  
Simone Magnino ◽  
Pier Giorgio Vigo ◽  
Iris Labalestra ◽  
Massimo Fabbi
Keyword(s):  
Polymerase Chain Reaction ◽  
Restriction Analysis ◽  
Simultaneous Detection ◽  
Bovine Herpesvirus ◽  
Chain Reaction ◽  
Bovine Herpesvirus 1 ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 1 ◽  
Polymerase Chain ◽  
Herpesvirus 4

A multiplex polymerase chain reaction (PCR) method coupled with a restriction analysis of PCR products (PCR with restriction fragment length polymorphism) was developed for the simultaneous detection of bovine herpesvirus 1, bovine herpesvirus 2, and bovine herpesvirus 4 infections. The specificity, sensitivity, and practical diagnostic applicability of this method were evaluated. This assay may be also adapted to the diagnosis of suid herpesvirus 1 and equine herpesviruses 1 and 3 and could become a powerful diagnostic tool.

scholarly journals Bovine herpesvirus 4 is tropic for bovine endometrial cells and modulates endocrine function

Reproduction ◽  
2007 ◽  
Vol 134 (1) ◽  
pp. 183-197 ◽  
Author(s):  
Gaetano Donofrio ◽  
Shan Herath ◽  
Chiara Sartori ◽  
Sandro Cavirani ◽  
Cesidio Filippo Flammini ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Stromal Cells ◽  
Bovine Herpesvirus ◽  
Endocrine Function ◽  
Uterine Disease ◽  
Endometrial Stromal Cells ◽  
Persistently Infected ◽  
Endometrial Cells ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 4

Bovinepostpartumuterine disease, metritis, affects about 40% of animals and is widely considered to have a bacterial aetiology. Although the γ-herpesvirus bovine herpesvirus 4 (BoHV-4) has been isolated from several outbreaks of metritis or abortion, the role of viruses in endometrial pathology and the mechanisms of viral infection of uterine cells are often ignored. The objectives of the present study were to explore the interaction, tropism and outcomes of BoHV-4 challenge of endometrial stromal and epithelial cells. Endometrial stromal and epithelial cells were purified and infected with a recombinant BoHV-4 carrying an enhanced green fluorescent protein (EGFP) expression cassette to monitor the establishment of infection. BoHV-4 efficiently infected both stromal and epithelial cells, causing a strong non-apoptotic cytopathic effect, associated with robust viral replication. The crucial step for the BoHV-4 endometriotropism appeared to be after viral entry as there was enhanced transactivation of the BoHV-4 immediate early 2 gene promoter following transient transfection into the endometrial cells. Infection with BoHV-4 increased cyclooxygenase 2 protein expression and prostaglandin estradiol secretion in endometrial stromal cells, but not epithelial cells. Bovine macrophages are persistently infected with BoHV-4, and co-culture with endometrial stromal cells reactivated BoHV-4 replication in the persistently infected macrophages, suggesting a symbiotic relationship between the cells and virus. In conclusion, the present study provides evidence of cellular and molecular mechanisms, supporting the concept that BoHV-4 is a pathogen associated with uterine disease.

scholarly journals Neurological disorder in cattle associated with bovine herpesvirus 4

2011 ◽  
Vol 63 (4) ◽  
pp. 828-835 ◽  
Author(s):  
E.A. Costa ◽  
A.C. Vasconcelos ◽  
M.R.Q. Bomfim ◽  
H.B. Amorim ◽  
G.B.L. Lima ◽  
...  
Keyword(s):  
Nested Pcr ◽  
Clinical Course ◽  
Bovine Herpesvirus ◽  
Pcr Assay ◽  
Bovine Herpesvirus 1 ◽  
Herpesvirus Type ◽  
Neurological Signs ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 1 ◽  
Herpesvirus 4

A nested PCR assay was used to diagnose bovine encephalitis through herpesviruses including bovine herpesvirus 5 (BHV-5), bovine herpesvirus 1 (BHV-1), Aujeszky's disease virus (SHV-1), and ovine herpesvirus 2 (OHV-2) in 14 fragments of central nervous system (CNS) from cattle that died with neurological signs. In addition, as some samples of bovine herpesvirus type 4 (BHV-4) have been isolated from neural tissue, it was also tested by nested PCR. The cases of encephalitis occurred in isolation at different times of the year and did not present any seasonality. The duration of the clinical course ranged between 1 to 15 days, and in 64.3% of the cases it manifested between 1 to 2 days. The most frequently observed neurological signs were ataxia, recumbency, unsteadiness and inability to stand, opisthotonus, paddling movements, nystagmus and ptyalism. In the nested assay, there was no evidence of: BHV-1, SHV-1 or OHV-2 in the DNA obtained from the CNS in any of the samples. But the presence of BHV-4 was found in all fragments of the CNS in cattle which died presenting neurological signs. Moreover, BHV-5 was found in association with BHV-4 in two of these samples.

scholarly journals Dot Immunobinding Assay for Detection of Bovine Herpesvirus 4 Antibodies in Rabbits

1999 ◽  
Vol 11 (3) ◽  
pp. 237-239 ◽  
Author(s):  
Mohamed Essmail ◽  
Dale Baker ◽  
James Collins ◽  
Sue Vandewoude ◽  
Mohamed Salman ◽  
...  
Keyword(s):  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 4

scholarly journals Molecular Characterization of the First Bovine Herpesvirus 4 (BoHV-4) Strains Isolated from In Vitro Bovine Embryos production in Argentina

PLoS ONE ◽  
2015 ◽  
Vol 10 (7) ◽  
pp. e0132212 ◽  
Author(s):  
Erika González Altamiranda ◽  
Julieta M. Manrique ◽  
Sandra E. Pérez ◽  
Glenda L. Ríos ◽  
Anselmo C. Odeón ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Bovine Herpesvirus ◽  
Bovine Embryos ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 4

scholarly journals Development and Evaluation of an Indirect ELISA: Serological Survey to Detect Specific Antibodies to Bovine Herpesvirus 4

2015 ◽  
Vol 58 (5) ◽  
pp. 725-731 ◽  
Author(s):  
Ernesto Renato Krüger ◽  
Tânia Regina Penha ◽  
Fernanda Cristina Hummelgen ◽  
Jorge Bacila Agottani ◽  
Demétrio Reva ◽  
...  
Keyword(s):  
Indirect Elisa ◽  
Bovine Herpesvirus ◽  
Serological Survey ◽  
Specific Antibodies ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 4
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