scholarly journals Design and Engineering of Next Generation Mammalian Cell Factories

2015 ◽  
Author(s):  
David James
Keyword(s):  
Mammalian Cell ◽  
Next Generation ◽  
Cell Factories

scholarly journals Next-generation of targeted AAVP vectors for systemic transgene delivery against cancer

2019 ◽  
Vol 116 (37) ◽  
pp. 18571-18577 ◽  
Author(s):  
Keittisak Suwan ◽  
Teerapong Yata ◽  
Sajee Waramit ◽  
Justyna M. Przystal ◽  
Charlotte A. Stoneham ◽  
...  
Keyword(s):  
Mammalian Cell ◽  
Neutralizing Antibodies ◽  
Structural Modification ◽  
Coat Proteins ◽  
Next Generation ◽  
Adeno Associated Virus ◽  
Targeted Gene Delivery ◽  
Human Solid Tumors ◽  
Transgene Delivery

Bacteriophage (phage) have attractive advantages as delivery systems compared with mammalian viruses, but have been considered poor vectors because they lack evolved strategies to confront and overcome mammalian cell barriers to infective agents. We reasoned that improved efficacy of delivery might be achieved through structural modification of the viral capsid to avoid pre- and postinternalization barriers to mammalian cell transduction. We generated multifunctional hybrid adeno-associated virus/phage (AAVP) particles to enable simultaneous display of targeting ligands on the phage’s minor pIII proteins and also degradation-resistance motifs on the very numerous pVIII coat proteins. This genetic strategy of directed evolution bestows a next-generation of AAVP particles that feature resistance to fibrinogen adsorption or neutralizing antibodies and ability to escape endolysosomal degradation. This results in superior gene transfer efficacy in vitro and also in preclinical mouse models of rodent and human solid tumors. Thus, the unique functions of our next-generation AAVP particles enable improved targeted gene delivery to tumor cells.

scholarly journals Systems biotechnology of mammalian cell factories

2008 ◽  
Vol 7 (2) ◽  
pp. 95-110 ◽  
Author(s):  
P. M. O'Callaghan ◽  
D. C. James
Keyword(s):  
Mammalian Cell ◽  
Systems Biotechnology ◽  
Cell Factories

Droplet size influences division of mammalian cell factories in droplet microfluidic cultivation

2016 ◽  
Vol 38 (2) ◽  
pp. 305-310 ◽  
Author(s):  
Prem Kumar Periyannan Rajeswari ◽  
Haakan N. Joensson ◽  
Helene Andersson-Svahn
Keyword(s):  
Mammalian Cell ◽  
Droplet Size ◽  
Cell Factories

Engineering mammalian cell factories with SINEUP noncoding RNAs to improve translation of secreted proteins

Gene ◽  
2015 ◽  
Vol 569 (2) ◽  
pp. 287-293 ◽  
Author(s):  
Laura Patrucco ◽  
Andrea Chiesa ◽  
Maria Felicia Soluri ◽  
Francesca Fasolo ◽  
Hazuki Takahashi ◽  
...  
Keyword(s):  
Mammalian Cell ◽  
Noncoding Rnas ◽  
Secreted Proteins ◽  
Cell Factories

scholarly journals Engineering Translation in Mammalian Cell Factories to Increase Protein Yield: The Unexpected Use of Long Non-Coding SINEUP RNAs

2016 ◽  
Vol 14 ◽  
pp. 404-410 ◽  
Author(s):  
Silvia Zucchelli ◽  
Laura Patrucco ◽  
Francesca Persichetti ◽  
Stefano Gustincich ◽  
Diego Cotella
Keyword(s):  
Mammalian Cell ◽  
Protein Yield ◽  
Cell Factories
Sign in / Sign up

Export Citation Format

Share Document