scholarly journals The use of molecular-biological research methods to detect the falsification of livestock products

2018 ◽  
Vol 20 (83) ◽  
pp. 417-419
Author(s):  
B.I. Nazar ◽  
H.V. Kushnir
Keyword(s):  
Research Methods ◽  
Molecular Genetic ◽  
Meat Products ◽  
Accurate Method ◽  
Poor Quality ◽  
Biological Research ◽  
Actual Problem ◽  
Heat Treated ◽  
Livestock Products ◽  
Gel Isoelectric Focusing

The article provides information on the use of molecular research methods to determine the species belonging to the protein of animal and plant origin, the discovery of genetically modified plants to prevent the falsification of products. Actual problem is the identification and implementation of histological researches and methods of PCR control of meat products, which allow differentiating the constituent components of samples, since all meat products, passing the stage of technological processing, and in the finished form mainly preserve their morphological features. The use of genetic technology for analyzing the quality of food products for humans and animal feeds is conditioned by the need for a sensitive, rapid and accurate method to prevent and detect falsifications. The complexity of determining the species composition of the protein is that the heat-treated forages (meat-bone meal, fishmeal, granulated feed, dry and canned feeds for cats and dogs) contains denatured proteins that have completely lost their specificity. Methods, such as immune diffusion in gel, isoelectric focusing, used for the identification of raw meat, in this case are unsuitable. The use of the RID method does not make it possible to determine the specificity of animal proteins already after a heat treatment at 80 °C. for 30 minutes. Meanwhile, using molecular genetic techniques, in particular PCR, one percent of beef can be detected, which was heat treated at 120 °C for 10 minutes after 30 cycles of amplification and 0.1% after 35 cycles. The introduction of screening and confirmatory methods of PCR detection of counterfeit foodstuffs, feeds and feed materials allows for effective and prompt detection of cases of fraud, preventing the entry into the circulation of poor quality products and feed, reducing the productivity and poisoning of animals and, as a consequence, obtaining safe and high-quality livestock products.

scholarly journals Evolutionary Analysis of Plastid Genomes of Seven Lonicera L. Species: Implications for Sequence Divergence and Phylogenetic Relationships

2018 ◽  
Vol 19 (12) ◽  
pp. 4039 ◽  
Author(s):  
Mi-Li Liu ◽  
Wei-Bing Fan ◽  
Ning Wang ◽  
Peng-Bin Dong ◽  
Ting-Ting Zhang ◽  
...  
Keyword(s):  
Molecular Genetic ◽  
Phylogenetic Reconstruction ◽  
Sequence Divergence ◽  
Genomic Analysis ◽  
Repeat Sequence ◽  
Biological Research ◽  
Comparative Genomic ◽  
Sequence Evolution ◽  
Evolutionary Analysis ◽  
Plastid Genomes

Plant plastomes play crucial roles in species evolution and phylogenetic reconstruction studies due to being maternally inherited and due to the moderate evolutionary rate of genomes. However, patterns of sequence divergence and molecular evolution of the plastid genomes in the horticulturally- and economically-important Lonicera L. species are poorly understood. In this study, we collected the complete plastomes of seven Lonicera species and determined the various repeat sequence variations and protein sequence evolution by comparative genomic analysis. A total of 498 repeats were identified in plastid genomes, which included tandem (130), dispersed (277), and palindromic (91) types of repeat variations. Simple sequence repeat (SSR) elements analysis indicated the enriched SSRs in seven genomes to be mononucleotides, followed by tetra-nucleotides, dinucleotides, tri-nucleotides, hex-nucleotides, and penta-nucleotides. We identified 18 divergence hotspot regions (rps15, rps16, rps18, rpl23, psaJ, infA, ycf1, trnN-GUU-ndhF, rpoC2-rpoC1, rbcL-psaI, trnI-CAU-ycf2, psbZ-trnG-UCC, trnK-UUU-rps16, infA-rps8, rpl14-rpl16, trnV-GAC-rrn16, trnL-UAA intron, and rps12-clpP) that could be used as the potential molecular genetic markers for the further study of population genetics and phylogenetic evolution of Lonicera species. We found that a large number of repeat sequences were distributed in the divergence hotspots of plastid genomes. Interestingly, 16 genes were determined under positive selection, which included four genes for the subunits of ribosome proteins (rps7, rpl2, rpl16, and rpl22), three genes for the subunits of photosystem proteins (psaJ, psbC, and ycf4), three NADH oxidoreductase genes (ndhB, ndhH, and ndhK), two subunits of ATP genes (atpA and atpB), and four other genes (infA, rbcL, ycf1, and ycf2). Phylogenetic analysis based on the whole plastome demonstrated that the seven Lonicera species form a highly-supported monophyletic clade. The availability of these plastid genomes provides important genetic information for further species identification and biological research on Lonicera.

scholarly journals Equine enterolithiasis

2017 ◽  
Vol 59 (2) ◽  
pp. 105
Author(s):  
N. DIAKAKIS (Ν.ΔΙΑΚΑΚΗΣ)
Keyword(s):  
Clinical Signs ◽  
Intestinal Bacteria ◽  
Accurate Method ◽  
Poor Quality ◽  
Surgical Removal ◽  
Daily Consumption ◽  
Abdominal Radiography ◽  
South West ◽  
History Of ◽  
Main Components

Enterolithiasis is characterized by the presence of enteroliths in the large colon of horses with the ascending colon being the most common site of obstruction. Enteroliths are composed of ammonium magnesium phosphate, which is supplied both by the digestive processes intestinal bacteria and by feeds. The enteroliths typically form around a central nidus. Although enterolithiasis is seen all over the world, the most cases are reported from North America, and more specifically, California, South West Indiana and Florida. As far as breed is concerned, it affects predominantly Arab horses and rarely Quarter and Thoroughbreds. As far as age is concerned, it is usually seen in middle-aged horses. Although the pathogenesis of enterolithiasis is not fully understood, nutrition and heritability are believed to be a part in it. A rich diet in ammonium, magnesium and phosphorus predisposes to enterolith formation, as those elements are the main components of enteroliths. Clinical signs vary considerably and are rarely characteristic of the disease. Usually, the presence of the enterolith is free of symptoms unless it leads to obstruction. In most cases of enterolithiasis a small amount of faeces, air and the administered mineral oil could pass from the obstruction site. On the contrary, in complete obstructions the passage is closed, defecation is absent and no laxative can pass the obstruction site. The enterolith is rarely found by rectal examination. A history of recurrent colic might be connected to the presence of enteroliths that cause partial or temporary obstruction. The most accurate method for diagnosing enterolithiasis is abdominal radiography. The treatment of choice is the surgical removal of enteroliths, which has a favorable prognosis provided that the laparotomy is going to take place early in course of the disease, before the onset of peritonitis. Intestinal rupture, which rapidly leads to peritonitis, is the gravest and commonest complication. Other complications are colitis, leakage through the laparotomy site and peritonitis. In order to prevent reformation of enteroliths, the daily consumption of alfalfa hay has to be reduced dramatically and poor quality hay has to be administered.

scholarly journals Genetic identification of bovine leukaemia virus

2018 ◽  
Vol 6 (2) ◽  
pp. 314-324 ◽  
Author(s):  
Irina Donnik ◽  
Irina Donnik ◽  
Ramil Vafin ◽  
Ramil Vafin ◽  
Aram Galstyan ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Research Methods ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Molecular Genetic ◽  
Genetic Research ◽  
Genetic Identification ◽  
Gene Fragment ◽  
Phylogenetic Classification ◽  
Pcr Rflp

Molecular genetic research methods make it possible to evaluate the genetic diversity of bovine leukemia virus (BLV) and are the most informative approaches to its genetic identification. Molecular genetic research methods work well for the phylogenetic analysis of sequenced nucleotide DNA sequences of the provirus, as well as for the polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP) according to the phylogenetic classification of the pathogen. The purpose of the research was to study the scientific and methodological approaches to the genetic identification of bovine leukemia virus, integrated into the molecular monitoring of infection of cattle with BLV genotypes. The authors used PCR-RFLP-genotyping and comparative phylogenetic analysis of aligned nucleotide sequences of the env gene fragment of the BLV provirus isolates to detect the genotypic affiliation of the cattle from twenty-one livestock farms of the Republic of Tatarstan. As a result, isolates of four out of ten BLV genotypes were found in the Tatarstani cattle, namely genotypes 1, 4, 7, and 8. The research involved a comparative analysis of 505 nucleotide sequences of a fragment of the BLV env gene, including those deposited in GenBank NCBI. The analysis confirms the inconsistency of several earlier PCR-RFLP typing strategies with the current approach in assessing the genotypic diversity by phylogenetic analysis. The improved strategy of PCR-RFLP genotyping of BLV corresponds with its modern phylogenetic classification. The strategy makes it possible to identify all the known genotypes of the viral pathogen. Its validity has been proved by in silico modelling of restrictogrammes and a phylogenetic analysis of the env gene fragment of 57 reference isolates of ten BLV genotypes that generate 57 genotype-associated combinations of diagnostically significant PCR-RFLP profiles.

scholarly journals PREVALENCE AND MOLECULAR GENETIC PECULIARITIES OF PARENTERAL VIRAL HEPATITIS B AND C AMONG HIVPOSITIVE CITIZENS OF THE FAR EASTERN FEDERAL DISTRICT, INCLUDING THOSE PERSONS SENTENCED TO DEPRIVATION OF FREEDOM

2019 ◽  
pp. 51-55
Author(s):  
E.A. Bazykina ◽  
V.B. Turkutyukov ◽  
O.E. Trotsenko ◽  
V.O. Kotova ◽  
L.A. Balakhonsteva ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Hiv Infection ◽  
Viral Hepatitis ◽  
Molecular Genetic ◽  
Federal District ◽  
Biological Research ◽  
Hiv Positive ◽  
Healthy Population ◽  
Genetic Characteristics ◽  
Viral Hepatitis B

We conducted a comparative analysis of the parenteral viral hepatitis B and C (HBV and HCV) prevalence and their molecular genetic characteristics among prisoners of persons diagnosed with HIV infection (41 samples), HIV-positive free citizens (187 samples) and «conditionally healthy population» with the lack of information about the presence of a diagnosis of chronic viral hepatitis of any etiology and HIV infection (231 samples). Immunological and molecular biological research methods were used. Obtained data analysis showed that the prevalence of infection markers with viruses of parenteral hepatitis was significantly higher in the groups of HIV-positive individuals (imprisoned and freemen). The HBsAg-negative form of the disease was determined among the HIV-positive free population and in the «conditionally healthy population». Over the past 10 years (2009–2018), the proportion of HIV-positive prisoners in custody of people with HCV monoinfection doubled, HBV was increased in 8.7 times. Significant decrease in the combined infection of HBV and HCV of this contingent was found. Given this decrease in the penitentiary system in HIV-positive individuals, the overall burden of HBV infection (both in mono form and coinfection with HCV) significantly (5.3 times) decreased , which can be attributed to successful widespread vaccination against hepatitis B in Russia. The most common HCV genotypes among HIV-positive individuals were 1b and 3a, genotypic structure of HBV prevailed genotype D.

scholarly journals A Method for Actin Filament Tracking in Fluorescent Microscopy Images

2020 ◽  
pp. paper37-1-paper37-10
Author(s):  
Danil Kononykhin ◽  
Valentina Berg ◽  
Andrey Krylov ◽  
Dmitry Sorokin
Keyword(s):  
Nearest Neighbor ◽  
Fluorescent Microscopy ◽  
Image Sequences ◽  
Biological Research ◽  
Tracking Accuracy ◽  
Actual Problem ◽  
Quality Metric ◽  
Ridge Detection ◽  
Research Areas ◽  
Microscopy Image

The automated tracking of subcellular structures in live microscopy image sequences is an actual problem in many biological research areas. A universal solution for this problem still does not exist due to a huge variety of data of different nature. In this work, we propose an algorithm for tracking actin filaments in 2D fluorescent image sequences. The filaments are moving in a random and abrupt manner frequently crossing each other. We used steerable filters based ridge detection followed by crossing filaments correction algorithm for filaments detection. The tracking was performed using a greedy nearest neighbor method. The quantitative evaluation of our approach was performed on several manually annotated image sequences using the object tracking quality metric MOTA. It was shown that the proposed approach outperforms an existing approach in tracking accuracy. In addition, the proposed approach allows processing crossed filaments, unlike the existing methods.

scholarly journals Outbreak of trichinellosis in eastern Slovakia

2009 ◽  
Vol 46 (4) ◽  
pp. 209-213 ◽  
Author(s):  
Z. Paraličová ◽  
J. Kinčeková ◽  
I. Schréter ◽  
P. Jarčuška ◽  
P. Dubinský ◽  
...  
Keyword(s):  
Clinical Signs ◽  
Meat Products ◽  
Meat Processing ◽  
Igg Antibodies ◽  
Raw Meat ◽  
Laboratory Parameters ◽  
Reactive Protein ◽  
Heat Treated ◽  
Periorbital Oedema ◽  
Laboratory Changes

AbstractTrichinellosis is a zoonosis caused by ingestion of undercooked raw meat from animals that harbour infectious larvae. In most of the Slovak regions there is ongoing life cycle of circulating trichinellosis in wild carnivores and wild boar population. The outbreak of trichinellosis occured in Rožňava district east Slovakia during spring in 2008. Ten members of farmer’s family and their relatives got ill while processing meat from home-made pig-slaughter for meals and meat products intended for wedding dinner. During the meat processing all of them tasted raw meat. Moreover, another 45 persons were exposed to this infection by eating heat-treated meat products. The most common predominant clinical signs were: myalgias, fever, fatigue, exanthema and periorbital oedema. On the 40th day after infection there were intermediate to high titres of trichinella IgG antibodies detected (10 patients), high levels of eosinophilia (10 patients) with maximum of 6.76 × 109/l (55 %) and profound changes in selected laboratory parameters: decreased levels of total proteins, increased levels of alpha 1-globulin and C reactive protein. Presence of IgG antibodies as well as aforementioned laboratory parameters was important markers of trichinellosis in our study, whereas other laboratory changes (leukocytosis, high levels of activity lactate dehydrogenase and creatine kinase) were detected only in few hospitalized patients.

scholarly journals Collaborative Trial for Validation of a Real-Time Reverse Transcriptase-Polymerase Chain Reaction Assay for Detection of Central Nervous System Tissues as Bovine Spongiform Encephalopathy RiskMaterial: Part 1

2006 ◽  
Vol 89 (5) ◽  
pp. 1335-1340
Author(s):  
Amir Abdulmawjood ◽  
Holger Schnenbrcher ◽  
Michael BÜlte
Keyword(s):  
Central Nervous System ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Meat Products ◽  
Spongiform Encephalopathy ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Heat Treated ◽  
Polymerase Chain

Abstract A collaborative trial was conducted to evaluate a real-time reverse transcriptase-polymerase chain reaction (RT-PCR) assay for detection of central nervous system (CNS) tissues in meat products (e.g., sausages). The method is based on the detection of ruminant glial fibrillary acidic protein (GFAP) mRNA by applying real-time RT-PCR. The assay was evaluated through a multicenter trial involving 12 participating laboratories that received coded cDNA obtained from 3 different types of sausages. The participants used 5 different real-time detection systems. The results obtained in this validation revealed that this real-time RT-PCR assay performed well in the different laboratories with a detection limit of at least 0.1% CNS in those test materials that contained strongly heat-treated samples (sausages cooked at 120C) and the medium heat-treated samples (sausages cooked at 80C). The detection limit of liver sausages was determined to be 0.2% of CNS. Neither the samples with no CNS additive nor the bovine DNA and the negative control containing 100% swine brain gave any positive signals. The presented results indicate that the real-time RT-PCR assay was just as reproducible between laboratories, as repeatable within a laboratory, could reliably be used for detection of bovine spongiform encephalopathy risk material in meat and meat products, and signify that it may be used with confidence in any laboratory.

scholarly journals Analysis of food quality perception processes.

1986 ◽  
Vol 34 (2) ◽  
pp. 227-230
Author(s):  
J.E.B.M. Steenkamp ◽  
B. Wierenga ◽  
M.T.G. Meulenberg
Keyword(s):  
Food Quality ◽  
Nutritive Value ◽  
Energy Content ◽  
Meat Products ◽  
Poor Quality ◽  
Important Indicator ◽  
Quality Perception ◽  
Quality Aspects ◽  
Perception Process ◽  
Minced Meat

A study was made to investigate ways in which consumers perceive the quality of food products and a model of the quality perception process was developed. Consumers used intrinsic (colour and appearance of product) and extrinsic (price and brand name) factors to determine rating of a product on quality aspects that cannot be evaluated at point of purchase (such as taste). The model was used on 13 foods and a high score for a food quality variable (nutritive value, energy content, additives and sensory quality) indicates that this quality is perceived as important. Energy content of meat, cheese, minced meat, margarine and meat products is perceived as most important indicator of quality. A high score for additives in jams, canned and jarred vegetables indicates that these products are perceived as poor quality. (Abstract retrieved from CAB Abstracts by CABI’s permission)

scholarly journals Monitoring of gluten in dairy products

2020 ◽  
Vol 22 (94) ◽  
pp. 8-12
Author(s):  
O. Haidei ◽  
S. Shuliak ◽  
I. Oleksiienko ◽  
G. Kyivska ◽  
O. Krushelnytska
Keyword(s):  
Celiac Disease ◽  
Dairy Products ◽  
Molecular Genetic ◽  
Monitoring Program ◽  
Meat Products ◽  
Gluten Free ◽  
Genetic Method ◽  
Gluten Content ◽  
The World ◽  
Haccp System

According to the World Gastroenterology Organization, the prevalence of celiac disease in the world is estimated at 1 in 300 people. According to unofficial statistics of the Celiac Disease Union, about 400,000 Ukrainian citizens have an individual intolerance to gluten. Given the large number of people with individual gluten intolerance and its only treatment – a lifelong diet, there is a need to monitor gluten in food, namely in dairy products. As not all manufacturers adhere to the HACCP system in good faith in their production, there is a risk of gluten entering the finished product. The aim of the study was to evaluate dairy products for gluten content. The article presents information on the results of monitoring gluten in dairy products (butter, margarine, kefir, sour milk cheeses, yogurt, hard and soft cheeses) producers of different regions of Ukraine by molecular genetic method in 2018–2020 using diagnostic R-Biopharm kits. According to research, it was found that 17 % of dairy products do not contain gluten, 83% contain from 2 to 5 mg/kg; 37.5 % of hard and soft cheeses, sweet cream butter, margarine do not contain gluten; 62.5 % contain gluten in the amount of 2 to 5 mg/kg, which is within acceptable limits for people with celiac disease. Studies have shown that a significant percentage of dairy products contain from 2 to 5 mg kg of gluten, which may indicate accidental entry into the final product or technical contamination. However, although these products are not certified as gluten-free and meet the requirements of current legislation. A significant range of products with a gluten content of up to 5 mg/kg encourages the implementation of the Gluten Control (Monitoring) Program in all products to increase the range for people with individual needs. Prospects for further research are to monitor meat products, semi-finished products, dietary products, baby food of domestic production for further analysis of compliance with current legislation and safety for people with individual intolerance to gluten.

scholarly journals Dietary Sodium Intake and the Main Sources of Salt in the Diet of Young Adults in Latvia

2018 ◽  
Vol 72 (2) ◽  
pp. 49-53
Author(s):  
Gunta Leite ◽  
Daiga Kunkulberga
Keyword(s):  
Research Methods ◽  
Sodium Intake ◽  
Meat Products ◽  
World Health Organisation ◽  
Dietary Sodium ◽  
World Health ◽  
Sociological Research ◽  
Statistical Research ◽  
Cereal Products ◽  
Salt Consumption

Abstract Scientific studies have regularly confirmed that nowadays the salt consumption through food is too much, and its consumption has to be reduced. The aim of the study was to ascertain the amount of salt consumed per day by 18–35 year-old Latvians as well as to identify the main sources of salt in their diets. The following research methods were used in the study: questionnaire based on an example recommended by the World Health Organisation, sociological research method, bread baking tests, and logically constructive, and statistical research methods. The results of this research showed that the average intake of salt in the diet of 18–35 year-old Latvians was 7.1 g per day. Of all the respondents, 63% consumed more than the recommended 5 g of salt per day, and none of them consumed less than necessary to meet their physiological needs. The results showed that women consumed less salt than men — approximately 6 g per day, while men consumed 8.2 g of salt per day. The main sources of salt in the diet of 18–35 year old Latvians were cereals and cereal products, as well as meat and meat products. Among cereal products, the key source of salt was represented by bread and pastry.

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