scholarly journals Conditional pathogenic microflora and its role in the eithiology of acute digestion disorders with features of diarrhea of newborned calves

2018 ◽  
Vol 20 (83) ◽  
pp. 304-307
Author(s):  
B.M. Kurtyak ◽  
M.S. Romanovych ◽  
T.O. Pundyak ◽  
L.V. Romanovych ◽  
G.V. Sobko ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Clostridium Perfringens ◽  
Chemotherapeutic Agents ◽  
New Drugs ◽  
Morphological Properties ◽  
Proteus Vulgaris ◽  
Mesenteric Lymph Nodes ◽  
Bacteriological Study ◽  
Digestive Disorders

The problem of treatment of acute digestive disorders with signs of diarrhea in calves remains relevant and important, as there are no sufficiently effective drugs, including antimicrobial effects. The low effectiveness of antibiotics and other chemotherapeutic agents and therapies in the vast majority of farms dictates the need to find new drugs. The purpose of our work was to study the significance of opportunistic microorganisms in the etiology of calving diarrhea. The research was conducted at farms of Lviv and Volyn regions. The incidence of newborn calves with acute digestive disorders with signs of diarrhea ranged from 36.8 to 100%, and mortality ranged from 17.4 to 30.8%. Bacteriological study was subjected to patmaterial from 20 dead calves at the age of 1–7 days. In this case, 88 cultures have been isolated from the internal organs, mesenteric lymph nodes, bone marrow, brain, walls and intestines. According to the culture – morphological properties, the cultures were classified into the following species: Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, Diplococcus capsulatus, Clostridium perfringens. In the bacteriological study of all microorganisms isolated - Diplococcus capsulatus 29.5%, Escherichia coli 30.7%, Pseudomonas aeruginosa 19.3%, Clostridium perfringens 11.4%, Proteus vulgaris 9.1%. Out of the 14 households, the causes of the disease of calves with acute gastrointestinal disorders in 35.7% of cases were certain opportunistic pathogens (Escherichia coli, Diplococcus capsulatus, Pseudomonas aeruginosa, Clostridium perfringens, Proteus vulgaris). In most farms (64.28%), the disease was caused by association (2, 3 and more) types of microbes. The pathogenic properties of the isolated cultures were tested on white mice. In determining the pathogenicity of isolated cultures from dead calves on white mice, the most pathogenic were microorganisms of the species Pseudomonas aeruginosa. Experimental mice (42.0%) died within 6–12 hours after infection. Highly pathogenic were mixed cultures of E. coli, diplococci, Pseudomonas aeruginosa, which killed 58.0% of infected mice 24–48 hours after infection. This indicates an increase in the pathogenic properties of microorganisms in the association. In order not to allow the passage of conditionally pathogenic microflora, it is necessary to adhere to the sanitary regime in dispensaries and maternity departments, and the terms of disinfection with variable sectional retention.

Антимікробна резистентність провідних збудників інфекцій сечових шляхів у Києві (Україна)

2017 ◽  
Vol 1 (2) ◽  
pp. 48-60
Author(s):  
A.G. Salmanov ◽  
A.V. Rudenko
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Staphylococcus Epidermidis ◽  
Klebsiella Oxytoca ◽  
Enterobacter Aerogenes ◽  
Enterobacter Cloacae ◽  
Proteus Vulgaris ◽  
Providencia Rettgeri ◽  
E Coli

Мета роботи — вивчити резистентність до антибіотиків бактеріальних збудників інфекцій сечових шляхів (ІСШ), виділених у пацієнтів урологічного стаціонару в м. Києві. Матеріали і методи. Досліджено 1612 штамів бактерій, виділених із сечі хворих з ІСШ (цистит, уретрит, пієлонефрит), госпіталізованих в урологічне відділення ДУ «Інститут урології НАМН України» у м. Києві протягом 2016 р. Серед пацієнтів переважали жінки — 1201 (74,5 %). Вік хворих становив від 17 до 74 років. Для збору даних використано медичну документацію лікарні. Мікробіологічні дослідження виконано у лабораторії мікробіології ДУ «Інститут урології НАМН України». Аналізували результати культурального дослідження зразків сечі, зібраних за наявності клінічних ознак ІСШ. Дослідження клінічного матеріалу та інтерпретацію отриманих результатів проводили загальноприйнятими методами. Вивчено чутливість уропатогенів до 31 антибіотика дискодифузійним методом відповідно до рекомендацій Інституту клінічних та лабораторних стандартів США (Clinical and Laboratory Standards Institute (CLSI)). Результати та обговорення. Аналіз мікробного спектра сечі виявив домінування серед уропатогенів штамів Escherichia coli (32,0 %), Enterococcus faecalis (19,5 %), Klebsiella pneumoniae (10,9 %), Staphylococcus epidermidis (8,9 %), S. haemolyticus (6,5 %) та Pseudomonas aeruginosa (6,4 %). Частка Enterococcus faecium, Enterobacter aerogenes і Streptococcus viridans становила відповідно 2,5, 2,2 і 1,6 %, Enterobacter cloacae, Klebsiella oxytoca, Acinetobacter baumannii, Proteus vulgaris та Providencia rettgeri — менше 1,0 %. У більшості випадків (69,7 %) мікроорганізми виділено у монокультурі, у решті випадків — у мікробних асоціа- ціях. Високу резистентність до тестованих антибіотиків виявили штами E. aerogenes (45,1 %), E. cloacae (45,7 %), E. faecium (40,9 %), E. faecalis (40,7 %), E. coli (39,9 %), P. aeruginosa (34,0 %), K. pneumoniae (28,6 %). Найбільш активними до уропатогенів були іміпенем (E. coli — 87,6 %, P. aeruginosa — 75,7 %, E. cloacae — 67,3 %, E. aerogenes — 72,6 %, K. pneumoniae — 93,2 %), меропенем (E. coli — 89,1 %, P. aeruginosa — 76,7 %, K. pneumoniae — 82,6 %), лефлоцин (E. coli — 74,5 %, ентерококи — 78,7 %, P. aeruginosa — 76,7 %, E. cloacae — 73,9 %, E. aerogenes — 80,4 %, K. pneumoniae — 83,5 %), амоксицилін/клавуланат (ентерококи — 84,6 %), фурагін (ентерококи — 82,6 %), цефоперазон (K. pneumoniae — 89,2 %, P. aeruginosa — 73,8 %), цефтріаксон (K. pneumoniae — 80,1 %). Висновки. Антибіотикорезистентність збудників ІСШ — важлива терапевтична проблема. Найбільшою активністю до уропатогенів характеризуються іміпенем, меропенем, лефлоцин, амоксицилін/ клавуланат, фурагін, цефоперазон, цефтріаксон, які можна розглядати як препарат вибору для призначення стартової терапії ІСШ. Необхідно здійснювати постійний моніторинг за резистентністю до дії антибіотиків. Політику використання антибіотиків у кожному стаціонарі слід визначати залежно від локальних даних щодо резистентності до протимікробних препаратів.

scholarly journals The Effect of Antimicrobial Drugs Tylocolinum, Tetragold and Cidisept-o on Escherichia coli Ultrastructure

2013 ◽  
Vol 2 (3) ◽  
pp. 65
Author(s):  
A. G. Shakhov ◽  
D. V. Fedosov ◽  
L. Y. Sashnina ◽  
O. V. Kazimirov
Keyword(s):  
Escherichia Coli ◽  
Bacterial Infections ◽  
Antimicrobial Agents ◽  
Water Solution ◽  
Uranyl Acetate ◽  
Chemotherapeutic Agents ◽  
Control Culture ◽  
New Drugs ◽  
Ultrastructural Changes ◽  
The Impact

<p>As a result of wide antibiotics, sulfonamides and other antimicrobial agents usage for the therapy of the animals with the bacterial infections caused by various causative agents including <em>Escherichia coli</em>, many microorganisms gained resistance to the chemotherapeutic agents. New combined drugs are being worked out during recent years, the components of which have various influence mechanisms on the bacterial cell that helps to provide resistance forming control. The results of the researches of the new antimicrobial agents, containing antibiotics in their composition, and non-antibiotic agent influence on the ultrastructure of <em>Escherichia coli</em> are represented in this study.</p> <p>5-hour <em>Escherichia coli 866</em> culture was processed by the drugs of the minimum bactericidal (Tylocolinum-0.39 µg/ml, Tetragold-6.25 µg/ml, Cidisept-o-25 µg/ml) and 4-time concentrations during 3 hours. Samples and control culture (without drugs) were fixed by the 2.5% glutaricdialdehyde on the s-Collidine Buffer, dehydrated in the ethanol with rising concentration, filled in epoxies. Ultrathin slices were stained by 2% water solution of uranyl acetate and lead citrate for 10 minutes. Then they were examined with the use of the electron microscope JEM-100 CX II by JEOL.</p> <p>The research showed deep ultrastructural changes in <em>Escherichia coli</em> cells under the antimicrobial agent influence determined by synergistic effect of combined Tylocolinum and Tetragold drugs components, possessing various bacteria influencing mechanisms, and aldehyde that is a component of Cidisept-o.</p> The electron microscopy usage allows to get unique information about the impact consequences of the traditional improved drugs and new drugs with antimicrobial activity on the bacterial infectious agents.

scholarly journals Anaerobes in human biliary tracts

1977 ◽  
Vol 6 (5) ◽  
pp. 494-498
Author(s):  
D M England ◽  
J E Rosenblatt
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Clostridium Perfringens ◽  
Antimicrobial Therapy ◽  
Total Group ◽  
Biliary Tract Surgery ◽  
Biliary Tracts ◽  
Group D ◽  
Aerobic And Anaerobic ◽  
Frequent Presence

During a 2-year period, 1,892 patients underwent biliary tract surgery at the Mayo Clinic. Both aerobic and anaerobic cultures of bile were performed in 371 of these patients. Sixty-nine percent of the cultures were positive, and 41% (117) of these grew anaerobes, although they were present in pure culture only twice. Mixed cultures most commonly contained four different organisms (three aerobes and one anaerobe). Bacteroides fragilis was the single most commonly isolated anaerobe and ranked fourth in terms of overall isolates behind Escherichia coli, group D streptococci, and Klebsiella B. fragilis accounted for 7.0% of the total group D streptococci, and Klebsiella. B. fragilis accounted for 7.0% of the total aerobic and anaerobic isolates and was present in 21% of all positive cultures. Pseudomonas aeruginosa and Clostridium perfringens ranked fifth and sixth, providing 6.5 and 5.9% of all isolates, respectively. This study demonstrates the frequent presence of anaerobes in patients with bactibilia and suggests that they be considered in the formulation of antimicrobial therapy for infections involving human biliary tracts.

scholarly journals Antibiotic Susceptibility of Bacteria Isolated from Abattoir Effluent-Impacted Tagangu River, Aliero, Kebbi State, North-Western Nigeria

2019 ◽  
pp. 1-8 ◽  
Author(s):  
B. G. Jega ◽  
O. O. Adebisi ◽  
S. S. Manga
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Antibiotic Susceptibility ◽  
Microbiological Quality ◽  
Most Probable Number ◽  
Resistant Bacteria ◽  
Proteus Vulgaris ◽  
Probable Number ◽  
Antibiotic Susceptibility Test ◽  
The Impact

This study aimed to evaluate the impact of abattoir effluent on microbiological quality of the receiving Tagangu River and the susceptibility of the isolates to commonly-used antibiotics. The most probable number (MPN) as well as the Kirby-Bauer method of antibiotic susceptibility test were used and demonstrated the total heterotrophic bacteria as well as Escherichia coli O157:H7 numbers in a total of 30 water samples collected over a period of three months at three strategic points of the river. In accordance with CLSI guidelines, four out of eight bacteria (Enterobacter sp., Pseudomonas aeruginosa, Proteus vulgaris and Citrobacter sp.) isolated, demonstrated multiple antibiotic resistance (MAR) against at least three out of septrin, chloramphenicol, amoxicillin, augmentin, gentamicin, tarivid and streptomycin. All the isolates (Escherichia coli, Klebsiella pneumoniae, Enterobacter sp., Pseudomonas aeruginosa, Proteus vulgaris, Citrobacter sp., Serratia marcescens and Aerobacter aerogenes) showed either high or intermediate susceptibility to sparfloxacin, ciprofloxacin and pefloxacin. The findings indicated that the river has been heavily polluted with the effluent discharges and did not meet any of the WHO guidelines for natural water sources fit for irrigation or other domestic purposes. As such, indiscriminate discharge of abattoir effluent could impact on the microbiological quality and promote increased incidence of multiple antibiotic resistant bacteria in a receiving river.

scholarly journals Profil Penggunaan Antibiotik dan Peta Kuman pada Pasien Gangren Diabetes Melitus di Sebuah RSUD di Kabupaten Gresik

2020 ◽  
Vol 3 (2) ◽  
pp. 96
Author(s):  
Isna Romadhona ◽  
Fauna Herawati ◽  
Rika Yulia
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Acinetobacter Baumannii ◽  
Proteus Mirabilis ◽  
Enterobacter Aerogenes ◽  
Enterobacter Cloacae ◽  
Proteus Vulgaris ◽  
Diabetes Melitus

Antibiotik merupakan obat yang digunakan untuk mengatasi dan mencegah infeksi bakteri. Penggunaan antibiotik yang tidak tepat dapat menimbulkan berbagai masalah, diantaranya pengobatan akan lebih mahal dan juga risiko terjadinya resistensi bakteri terhadap antibiotik. Penelitian ini bertujuan untuk mengetahui profil penggunaan antibiotik dan profil peta kuman pada pasien gangren diabetes melitus di sebuah RSUD di Kabupaten Gresik serta untuk mengetahui kesesuaian penggunaan antibiotik dengan mengacu pada Permenkes Republik Indonesia No. 2406/Menkes/PER/XII/2011. Data penggunaan antibiotik diperoleh dari catatan Rekam Medis pada periode Januari – November 2017. Data penggunaan antibiotik dihitung dengan menggunakan rumus DDD/100 pasien-hari rawat. Hasil perhitungan DDD/100 pasien-hari rawat menunjukkan hasil sebesar 470,11 DDD/100 pasien-hari rawat. Peta kuman pada pasien gangren, melaporkan adanya bakteri Enterobacter cloacae 24%, Escherichia coli 18%, Staphylococcus aureus 15%, Acinetobacter baumannii 9%, Pseudomonas aeruginosa 6%, Citrobacter youngae 6%, Enterobacter aerogenes 6%, Proteus vulgaris 6%, Staphylococcus schleiferi 6%, Klebsiella pneumoniae 3%, dan Proteus mirabilis 3% . Penggunaan antibiotik seftriakson dan metronidazol pada pasien gangren diabetes melitus di sebuah RSUD di Kabupaten Gresik pada periode Januari – November 2017 telah sesuai dengan pedoman penggunaan antibiotik berdasarkan Permenkes Republik Indonesia No. 2406/Menkes/PER/ XII/2011, yaitu antibiotik golongan sefalosporin generasi III yang lebih aktif terhadap Enterobacteriaceae dan antibiotik golongan nitroimidazol yang dapat mengobati infeksi bakteri basil anerob Gram-Negatif.

scholarly journals تأثير المستخلصات المائية لنبات الرينش البرقاوي Arum cyreniacum على بعض أنواع البكتيريا السالبة والموجبة لصبغة جرام

2020 ◽  
Vol 35 (1) ◽  
pp. 60-68
Author(s):  
أحمد أمراجع عبدالرازق ◽  
سامي محمد صالح
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Sp ◽  
Proteus Vulgaris

 ارتبطت تسمية نبات الرينش البرقاوي Arum cyreniacum بأقليم برقة Cyrenaica موطنه الأصلي والوحيد وخاصة منطقة الجبل الأخضر, ويعتبر أحد نباتاتها المعروفة التي استخدمت قديماً لأغراض غذائية وطبية, ونظرا للتوجه الحديث نحو استخدام النباتات الطبية في مجال المقاومة الحيوية, استهدفت هذه الدراسة اختبار الفاعلية التثبيطية للمستخلصات المائية الباردة والساخنة لنبات الرينش البرقاوي بعدة تراكيز (100, 200, 300, 400) ملغم/ مل ضد ثلاثة أنواع من البكتيريا السالبة (Escherichia coli, proteus vulgaris, Pseudomonas aeruginosa) ونوعين من البكتيريا الموجبة (Staphylococcus arueus, Bacillus sp.) الممرضة للإنسان واختبار حساسيتها بطريقة الحفر في الأجار, ومقارنتها بأقراص المضاد الحيوي Tetracycline. بينت النتائج أن المستخلصات المائية لنبات الرينش البرقاوي تمتلك فاعلية تثبيطية جيدة ضد البكتيريا المدروسة, وأن التركيز 400 ملغم / مل كان الأكثر فاعلية في تسجيل أعلى تثبيط مقارنة بالمضاد الحيوي تراوحت مابين (8.2-15) ملم, ولم يظهر التركيز 100 ملغم/ مل أي تأثير تثبيطي على جميع أنواع البكتيريا باستثناء بكتيريا Bacillus sp., في حين كانت بكتيريا  coli Escherichia وPseudomonas aeruginosa هي الأكثر مقاومة للمضاد الحيوي, كما أشارت النتائج إلى الكفاءة العالية للمستخلص البارد في تثبيط البكتيريا السالبة والمستخلص الساخن في تثبيط البكتيريا الموجبة.

scholarly journals Bacteriological Analysis of Beef Production Chain in Birnin Kebbi Metropolis of Kebbi State, Nigeria

2020 ◽  
pp. 64-76
Author(s):  
Famubo, Joseph A. ◽  
Isiaka, Aishatu ◽  
Abbas, Yusuf B.
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Streptococcus Pyogenes ◽  
Micrococcus Luteus ◽  
Klebsiella Pneumonia ◽  
Proteus Vulgaris ◽  
Production Chain ◽  
Retail Outlets ◽  
Retail Meat

The hygiene status of most abattoirs and retail outlets in Nigeria is very poor, and it contributes to the unacceptable level of bacteria load in beef, and poses a health risk to consumers. This study examined the bacteriological analysis of beef production chain in Birnin Kebbi metropolis. A total of 100 samples of meat, water and surface swab in Main Market, GRA, Badariya, Bayan Kara and Rafin Atiku, Birnin Kebbi Central abattoir and retail meat shops were collected aseptically, processed and analyzed. Meat from retail outlets were mostly contaminated by Staphylococcus aureus (24.4%), followed by Escherichia coli (17.3%), Klebsiella pneumonia (9.8%), Salmonella typhimurium (9.1%), Entrobater aerogenes (8.5%), Streptococcus pyogenes (8.1%), Proteus vulgaris (7.8%), Micrococcus luteus (7.8%) and Pseudomonas aeruginosa (7.2%). In the abattoirs contact surfaces, contamination occurred mostly by Staphylococcus aureus (23.4%), Salmonella typhimurium (18.2%), Escherichia coli (16.9%), Entrobater aerogenes (9.1%), Streptococcus pyogenes (7.8%), Klebsiella pneumonia, Proteus vulgaris, and Pseudomonas aeruginosa (6.5% each) and Micrococcus luteus (5.2%). The findings revealed a high bacterial load in both abattoir and retail meat outlets. In order to safeguard the health of the public against the risks of food borne infections, there is a need to educate and advocate good sanitation and meat handling practices in the abattoir and beef retail outlets.

scholarly journals IN VITRO EVALUATION OF ANTIMICROBIAL ACTIVITY OF MICRO PROPAGATED CALLUS OF CURUULIGO ORCHIODES (BLACK MUSILLI) AGAR WELL DIFFUSION AND MINIMUM INHIBITORY CONCENTRATION (MIC)

2017 ◽  
Vol 9 (3) ◽  
pp. 75
Author(s):  
Rajanikanth Garapati ◽  
N. Ramesh
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Candida Albicans ◽  
Bacillus Cereus ◽  
Proteus Vulgaris ◽  
Methanol Extracts ◽  
Tract Infections

Objective: In vitro investigated the potential of methanol extracts of micro-propagated C. orchiodes in the antimicrobial property against the three gram-negative bacteria, two gram-positive and one fungal filament.Methods: The micro propagated callus methanol extract was examined against Escherichia coli, Proteus vulgaris, Salmonella typhimurium, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus and Candida albicans. The zone of inhibitions are determined at 10 mg/ml concentration of methanol extracts of callus on agar well plate and MIC against tested microorganism.Results: The highest antibacterial activity recorded in Staphylococcus aureus Bacillus cereus and followed by Candida albicans. Antibacterial activity of leaf extracts of A. reticulata was also significant against the tested microorganisms Escherichia coli, Salmonella typhi, Proteus vulgaris, Pseudomonas aeruginosa compared to ciprofloxacin.Conclusion: Based on the above observations, these extracts were further evaluated for their effect on microorganisms causing infections like typhoid fever, urinary tract infections, septicemia, toxic shock syndrome, skin infection, nosocomial infection, arthritis and diarrhoea. The results also suggest that these plants serve a therapeutic purpose in the treatment bacterial infections.

The Antimicrobial Activity of Extracts of the Lichen Cetraria aculeata and Its Protolichesterinic Acid Constituent

2003 ◽  
Vol 58 (11-12) ◽  
pp. 850-854 ◽  
Author(s):  
Ayşen Özdemir Türk ◽  
Meral Yılmaz ◽  
Merih Kıvanç ◽  
Hayrettin Türk
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Listeria Monocytogenes ◽  
Bacillus Cereus ◽  
Aeromonas Hydrophila ◽  
Proteus Vulgaris ◽  
Ethanol Extracts

Abstract In this study, the antimicrobial activity of the acetone, diethyl ether and ethanol extracts of the lichen Cetraria aculeata has been investigated. The extracts were tested against twelve bacteria and eight fungi and found active against Escherichia coli, Staphylococcus aureus, Aeromonas hydrophila, Proteus vulgaris, Streptococcus faecalis, Bacillus cereus, Bacillus subtilis, Pseudomonas aeruginosa, Listeria monocytogenes. No antimicrobial activity against the fungi was detected. It was determined that only one substance in the extracts has antimicrobial activity and it was characterized as protolichesterinic acid. The MICs of the extracts and protolichesterinic acid were also determined.

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