scholarly journals Association of allele variants of IGF-I, GH and PIT-1 genes with production traits of Birkivska Barvista chicken breed

2017 ◽  
Vol 19 (79) ◽  
Author(s):  
R. O. Kulibaba
Keyword(s):  
Production Traits ◽  
Indel Polymorphism ◽  
Chicken Breed ◽  
Promoter Fragment ◽  
Pcr Rflp ◽  
Igf I ◽  
Gh Gene ◽  
Number Of Individuals ◽  
Transcription Factor 1 ◽  
I Gene

The article considers the questions about association between allelic variants of insulin-like growth factor I (IGF-I), growth hormone (GH) and pituitary transcription factor-1 (PIT-1) genes with the egg productivity of the Borkovskaya Barvistaya chicken breed. Using classical PCR and PCR-RFLP methods, the PstI-polymorphism in the 5'UTR fragment of the IGF-I gene, HinfI-polymorphism in the promoter fragment of IGF-I gene, AluI-polymorphism in the fourth intron of the GH gene and indel polymorphism in the second intron of the PIT-1 gene were determined. As a result of the studies the significant differences in egg weight values on 30 weeks of life for HinfI-polymorphism in the promoter fragment of the IGF-I gene were revealed. For individuals with the C/C genotype was specific the large values of this index compared to A/A (51,7 ± 0,61 vs. 47.9 ± 0,92 respectively). For PstI-polymorphism in the 5'UTR of the IGF-I gene was shown the prevalence in the number of eggs for 12 weeks of productivity for individuals with the C1C1 genotype compared to C2C2 (65,2 ± 3,77 vs. 60,9 ± 1,33), but in this case the differences are not significantly, what can be caused by a small number of individuals with C1C1 genotype (12). There were no significant differences for other loci.

Effects of polymorphism in the 5′-flanking region of the IGF-I gene on milk-production traits in Chinese Holstein cattle

2012 ◽  
Vol 52 (9) ◽  
pp. 795 ◽  
Author(s):  
M. A. Alim ◽  
Yan Xie ◽  
Yipeng Fan ◽  
Xiaoping Wu ◽  
Yi Zhang ◽  
...  
Keyword(s):  
Milk Production ◽  
Holstein Cattle ◽  
Production Traits ◽  
Milk Production Traits ◽  
Chinese Holstein ◽  
Chinese Holstein Cattle ◽  
Igf I ◽  
Flanking Region ◽  
Fat Yield ◽  
I Gene

Milk and milk products are major components of human dietary intake and have a potential influence on health. In our experiment, associations between insulin-like growth factor 1 (IGF-I) gene polymorphism and milk-production traits were analysed in Chinese Holstein cattle. A polymorphism, transition at position g.1407 T > C, was identified in the 5′-flanking region of the IGF-I gene by pooled DNA sequencing. The identified single-nucleotide polymorphism (SNP) was genotyped by matrix-assisted laser desorption–ionisation time-of-flight mass spectrometry (MALDI–TOF MS) methods from 752 individuals. Significant associations between IGF-I genotypes and 305-day milk yield, fat yield and protein yield were found. Homozygous cows with TT genotype showed the highest milk, fat and protein yields, with increases of 532.75 kg, 23.57 kg and 14.69 kg, respectively, as compared with homozygous CC cows. Heterozygous CT cows had intermediate yields. Allele substitution showed that the C allele decreased milk yield (255.23 kg), fat yield (11.37 kg) and protein yield (7.05 kg), whereas it increased protein percentage (0.01%). The results suggest that IGF-I is a candidate gene that influences milk-production traits, and provides useful information to be implemented in breeding programs to improve the performance of the Chinese Holstein.

scholarly journals Associations between polymorphisms of growth hormone releasing hormone (<i>GHRH</i>) and pituitary transcription factor 1 (<i>PIT1</i>) genes and production traits of Limousine cattle

2003 ◽  
Vol 46 (6) ◽  
pp. 527-534 ◽  
Author(s):  
A. Dybus ◽  
M. Kmieć ◽  
Z. Sobek ◽  
W. Pietrzyk ◽  
B. Wiśniewski
Keyword(s):  
Growth Hormone ◽  
Transcription Factor ◽  
Bovine Growth Hormone ◽  
Growth Hormone Releasing Hormone ◽  
Production Traits ◽  
Releasing Hormone ◽  
Pcr Rflp ◽  
Transcription Factor 1

Abstract. Associations between polymorphism of the bovine growth hormone releasing hormone (GHRH) and pituitary transcription factor 1 (PIT1) genes and production traits of Limousine cattle were analysed. A total of 130 calves were included in the study. PCR-RFLP method was used for genotyping. The frequencies of genotypes and alleles of PIT1 and GHRH were as follows: 0.0692 – AA, 0.4077 – AB, 0.5231 – BB, and 0.2731 for PIT1A, 0.7269 for PIT1B; 0.0154 – AA, 0.1692 – AB, 0.8154 – BB, and 0.1 for GHRHA, 0.9 for GHRHB. Associations between polymorphism and production traits of Limousine calves were found. Statistically significant differences (P ≤ 0.01) between individuals of different GHRH genotypes were found in relation to height at sacrum (cm) and height at withers (cm) at 210th day of age. The calves with AA genotype of GHRH were shorter (−8,14 and −8,33 cm) than AB and BB individuals (P ≤ 0.01). The small number of calves with the AA genotype did not enable important conclusions.

scholarly journals Identification of Locus GH/Alui Polymorphisms of Kuantan and Pesisir Cattle

2020 ◽  
Vol 44 (3) ◽  
Author(s):  
Hidayati Hidayati ◽  
Robbana Saragih
Keyword(s):  
Beef Cattle ◽  
Gene Locus ◽  
River Flow ◽  
Incubation Temperature ◽  
Gene Diversity ◽  
Dna Templates ◽  
Pcr Rflp ◽  
Gh Gene ◽  
West Sumatra ◽  
I Gene

Kuantan cattle is a local beef cattle of Riau Province that adaptive and breed well along the Kuantan river flow, found in Indragiri Hulu Regency and Kuantan Singingi Regency, have smaller morphometric performance compared to other local beef cattle and are relatively the same as Pesisir cattle. One of the genetic markers of growth determinant in beef cattle is the GH/AluI gene locus. Identification of the diversity of the GH/Alu I gene was carried out using the PCR-RFLP. Fifty four samples of Kuantan cattle consisting of 25 samples from Indragiri Hulu Regency and 29 samples from Kuantan Singingi Regency, as well as 25 samples of Pesisir cattle from BPTU-HPT Padang Mengatas Payakumbuh, West Sumatra, were used in this study. The oligonucleotida forward F-5’GCTGCTCCTGAGGGCCCTTC-3’ and reverse R-5’CATGACCCTCAGGTACGTCTCCG-3’ used in this study along 211 bp of GH Gene. The PCR mixture consisted of DNA templates 20 - 25 ng, 0.4 µL  primer forward dan primer reverse (10 ng primer forward and primer reverse), 25 µL dream tag green master mix and ddH2O up to a 50 µL. PCR machine conditions consisted of 940C pradenaturation for 5 minutes, 940C denaturation for 30 seconds, annealing 650C for 1 minute, 720C extension for 50 seconds and 720C final extension for 5 minutes. Identification of GH gene diversity using AluI at an incubation temperature of 370C for 3 hours. The results in this study were found monomorphic of GH/AluI genes in Kuantan and Pesisir cattle. The monomorphic in Kuantan cattle can be used as an indicator to assess the purity of Kuantan cattle in this areas. 

GHR and IGF-I gene expression and production characteristics associated with GH gene polymorphism in Nile tilapia

Aquaculture ◽  
2015 ◽  
Vol 435 ◽  
pp. 195-199 ◽  
Author(s):  
Fernanda Tanamati ◽  
Stefânia Caroline Claudino da Silva ◽  
Maria Del Pilar Rodriguez Rodriguez ◽  
Guilherme Pereira Schuroff ◽  
Carlos Souza do Nascimento ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Polymorphism ◽  
Nile Tilapia ◽  
Igf I ◽  
Gh Gene ◽  
I Gene ◽  
Production Characteristics

468: Polymorphic CA Repeats in IGF-I Gene is a Common Genetic Modifier in the Etiology of Prostate Cancer and Benign Prostatic Hyperplasia

2004 ◽  
Vol 171 (4S) ◽  
pp. 125-125
Author(s):  
Lizhong Wang ◽  
Kazunari Sato ◽  
Norihiko Tsuchiya ◽  
Chikara Ohyama ◽  
Shigeru Satoh ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Benign Prostatic Hyperplasia ◽  
Genetic Modifier ◽  
Prostatic Hyperplasia ◽  
Igf I ◽  
I Gene

Regulation of insulin-like growth factor-I gene expression by growth factors in cultured vascular smooth muscle cells

1990 ◽  
Vol 125 (3) ◽  
pp. 381-386 ◽  
Author(s):  
K. E. Bornfeldt ◽  
H. J. Arnqvist ◽  
G. Norstedt
Keyword(s):  
Gene Expression ◽  
Smooth Muscle ◽  
Growth Factor ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Basic Fgf ◽  
Aortic Smooth Muscle ◽  
Factor I ◽  
Igf I ◽  
I Gene

ABSTRACT The aim of this investigation was to study the regulation of insulin-like growth factor-I (IGF-I) gene expression in cultured rat aortic smooth muscle cells. Near-confluent cells were deprived of serum for 24 h and then exposed to IGF-I, insulin, serum, basic fibroblast growth factor (basic FGF), platelet-derived growth factor (PDGF-BB; consisting of B-chain homodimer) or GH for 24 h. Levels of IGF-I mRNA were measured by solution hybridization. The level of IGF-I mRNA was markedly decreased by 10% (v/v) newborn calf serum (78 ± 4 (s.e.m.) % decrease), 1 nmol basic FGF/1 (53 ± 8%), and 1 nmol PDGF-BB/1 (40 ± 3%) when measured after 24 h. The effect of PDGF-BB was significant after 6 h and became more marked after 24 h. GH (1 nmol/l or 0.1 μmol/l or insulin (1 nmol/l had no effect after 24 h, whereas IGF-I (1 nmol/l and insulin (10 μmol/l increased IGF-I mRNA 64 ± 20% and 46±14% respectively. The increase caused by IGF-I was demonstrated after 3 h, and was most marked after 24 h. Using Northern blot analysis of cultured aortic smooth muscle cells, IGF-I transcripts of 7-4, 1.7 and 1.1–0.8 kilobases were observed. Exposure of the cells to 10% serum, 1 nmol basic FGF/1 or 1 nmol PDGF-BB/1 for 48 h increased the cell number by 104 ±7%, 64 ± 3% and 61±22% respectively, while IGF-I, insulin and GH had little effect. In conclusion, IGF-I, and high concentrations of insulin, increased IGF-I mRNA in vascular smooth muscle cells, whereas factors which were stronger mitogens decreased IGF-I gene expression. Journal of Endocrinology (1990) 125, 381–386

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