scholarly journals Визначення поліморфних варіантів фрагментів ДНК збудників хламідійних інфекцій сільськогосподарських тварин

2017 ◽  
Vol 19 (73) ◽  
pp. 66-70
Author(s):  
M.V. Korniyenko ◽  
І.М. Кsyonz
Keyword(s):  
Dna Sequences ◽  
Genetic Test ◽  
Molecular Genetic ◽  
Nucleotide Sequences ◽  
Farm Animals ◽  
Gene Encoding ◽  
Oligonucleotide Primers ◽  
Test Systems ◽  
Molecular Genetic Test ◽  
Chlamydial Infections

Under the currently existing classification, adopted at the Second European Symposium «Animal Chlamydioses and Zoonotic Implications (EMAC-2)», Chlamydia pathogens of animals and humans are intracellular gram-negative bacteria belonging to Chlamydiales order, Chlamydiaseae family, Chlamydia genus. The above mentioned genus includes 11 species: C. abortus, C. avium, C. caviae, C. felis, C. gallinacea, S. muridarum, C. pecorum, C. pneumoniae, C. psittaci, C. suis and C. trachomatis, 10 of them being pathogenic for animals and Chlamydia trachomatis being exclusively human Chlamydiosis agent. Development of highly sensitive and specific molecular genetic test systems for indication and species differentiation of the said Chlamydia genus bacteria will permit to reliably study various aspects of chlamydial infection. Aim of the study was to perform bioinformatiс alignment of different genes’ nucleotide sequences to determine polymorphic DNA regions of Chlamydia genus bacteria, which are the basis for designing oligonucleotide primers for molecular genetic test systems capable of differentiating pathogens, causing disease in cattle and small ruminants, horses and pigs, by species. Bioinformatic study has been performed in 411 primary nucleotide DNA sequences of genes encoding 16S rRNA, RNase P RNA and MOMP of four chlamydial infections agents of farm animals (C. abortus, C. pecorum, C. pneumoniea, C. suis) obtained from the international electronic databases «GenBank» and «PubMed». Meanwhile, it was determined that the gene encoding the main outer membrane protein (MOMP) of Chlamydia possesses the highest variability level, making 97.1%. Using the method of aligning the primary sequences of the said gene, by means of «MEGA4» and «MEGA7» software, polymorphic fragments of nucleotide DNA sequences have been determined for C. abortus, C. pecorum, C. pneumoniea and C. suis. Specificity testing of polymorphic fragments, determined for each of the four chlamydia species with nucleotide sequences of microorganisms, both opportunistic and other infections pathogens, was carried out using «Blast» online software applications. Polymorphic fragments of the gene encoding MOMP of the above Chlamydia genus bacteria will be used to design oligonucleotide primers of test systems for indication and specific differentiation of farm animals’ chlamydial infections agents in polymerase chain reaction.

scholarly journals Development of a Web-Based Query Tool for Quality Assurance of Clinical Molecular Genetic Test Results

2007 ◽  
Vol 9 (1) ◽  
pp. 95-98 ◽  
Author(s):  
Matthew J. McGinniss ◽  
Rebecca Chen ◽  
Victoria M. Pratt ◽  
Arlene Buller ◽  
Franklin Quan ◽  
...  
Keyword(s):  
Quality Assurance ◽  
Genetic Test ◽  
Molecular Genetic ◽  
Test Results ◽  
Web Based ◽  
Genetic Test Results ◽  
Query Tool ◽  
Molecular Genetic Test

scholarly journals Effective communication of molecular genetic test results to primary care providers

2012 ◽  
Vol 15 (6) ◽  
pp. 444-449 ◽  
Author(s):  
Maren T. Scheuner ◽  
◽  
Maria Orlando Edelen ◽  
Lee H. Hilborne ◽  
Ira M. Lubin
Keyword(s):  
Primary Care ◽  
Genetic Test ◽  
Molecular Genetic ◽  
Effective Communication ◽  
Primary Care Providers ◽  
Test Results ◽  
Care Providers ◽  
Genetic Test Results ◽  
Molecular Genetic Test

Discrepant DNA analysis in three patients with inherited arrhythmia: Molecular genetic test results deserve a second glance

2008 ◽  
Vol 146A (11) ◽  
pp. 1466-1469 ◽  
Author(s):  
Christina R. Honeywell ◽  
Michael H. Gollob ◽  
Julie Rutberg ◽  
Robert M. Gow ◽  
Michael T. Geraghty
Keyword(s):  
Dna Analysis ◽  
Genetic Test ◽  
Molecular Genetic ◽  
Test Results ◽  
Genetic Test Results ◽  
Inherited Arrhythmia ◽  
Molecular Genetic Test

scholarly journals Improving Molecular Genetic Test Utilization through Order Restriction, Test Review, and Guidance

2015 ◽  
Vol 17 (3) ◽  
pp. 225-229 ◽  
Author(s):  
Jacquelyn D. Riley ◽  
Gary W. Procop ◽  
Kandice Kottke-Marchant ◽  
Robert Wyllie ◽  
Felicitas L. Lacbawan
Keyword(s):  
Genetic Test ◽  
Molecular Genetic ◽  
Order Restriction ◽  
Test Review ◽  
Molecular Genetic Test

Relative accuracy of the halothane challenge test and a molecular genetic test in detecting the gene for porcine stress syndrome

1993 ◽  
Vol 71 (6) ◽  
pp. 1395-1399 ◽  
Author(s):  
William E. Rempel ◽  
MingYu Lu ◽  
Sayed el Kandelgy ◽  
Catherine F. H. Kennedy ◽  
Lisa R. Irvin ◽  
...  
Keyword(s):  
Genetic Test ◽  
Molecular Genetic ◽  
Challenge Test ◽  
Relative Accuracy ◽  
Stress Syndrome ◽  
Porcine Stress Syndrome ◽  
Molecular Genetic Test

scholarly journals The refinement of species affiliation of some click beetles (Coleoptera: Elateridae) based on the molecular genetic analysis

2021 ◽  
Vol 101 (1) ◽  
pp. 14-19
Author(s):  
T.M. Bragina ◽  
◽  
D.T. Konysbayeva ◽  
M.M. Rulyeva ◽  
M.A. Bobrenko ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Dna Sequences ◽  
Sandy Loam ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Nucleotide Sequences ◽  
Time Data ◽  
Click Beetles ◽  
Gene Coi ◽  
University Of Oslo

The article is devoted to data on the approbation of modern methods and the refinement of species affiliation of soil-inhabiting larvae of click beetles (wireworm) based on the molecular genetic analysis (DNAbarcoding). For the first time, data obtained on the complete identity of certain DNA sequences of a number of species of click beetles living in the Kostanay Region (Kazakhstan) — dangerous pests of agricultural crops. At the same time, the World genetic bank (GenBank) did not find identical DNA sequences of decoded DNA nucleotide sequences for a number of studied specimens. The basis for this study was the materials collected in 2018 in the subzone of ordinary black earth on sandy loam soils (Mendykarinsky district). The selection of larvae was carried out by the method of standard soil-zoological samples. The fixation and storage of the selected click beetles larvae was carried out according to the method of preparing samples for molecular genetic analysis with fixation in 96 % alcohol. After the classic identification of the taxonomic position of the collected specimens, the species were identified by genetic analysis on the nucleotide sequence of the cytochrome C oxidase I subunit gene (COI). The assembly and decoding of the DNA nucleotide sequences of the studied samples were carried out using the programs «Codon Code Aligner» and «MEGA-X». As a result of the work carried out in the DNA laboratory of the Museum of Natural History (University of Oslo, Norway), it was possible to identify the complete identity of the DNA sequences of several mass species of click beetles, while a number of decoded DNA sequences of model specimens were absent in the genetic bank, which requires replenishment in it with new data.

scholarly journals PCR test systems for the Clavichlamydia salmonicola and Piscichlamydia salmonis detection in fish

2019 ◽  
Vol 5 (1) ◽  
pp. 20-26
Author(s):  
V. K. Zezekalo ◽  
S. B. Peredera ◽  
T. V. Buslyk ◽  
K. F. Pochernyaev ◽  
N. S. Shcherbakova
Keyword(s):  
Skin Diseases ◽  
Molecular Genetic ◽  
Species Differentiation ◽  
Fish Skin ◽  
Fish Farms ◽  
Test Systems ◽  
Commercially Important ◽  
Available Information ◽  
Pcr Test ◽  
Chlamydial Infections

The aim of our work was to develop PCR test systems for the identification and differentiation of the Piscichlamydia salmonis and Clavochlamydia salmonicola, species, that are known epitheliocystis infection agents of gill and fish skin diseases, characterized by the presence of specific ‘inclusions’ in the epithelial cells of the gills. To date, the diseases of fish associated with chlamydial infections have been detected in more than 90 species of freshwater and marine fish worldwide. For now, there is no available information on the prevalence of Piscichlamydia salmonis and Clavochlamydia salmonicola, which can cause epitheliocystis of commercially important aquaculture species in Ukraine. Identification of these pathogens is possible only using molecular genetic methods. As a result of our research, we got PCR tests for the identification and species differentiation of Piscichlamydia salmonis and Clavochlamydia salmonicola. The use of diagnostics for the identification of Piscichlamydia salmonis and Clavochlamydia salmonicola makes chlamydial infections monitoring among various fish species possible and it will increase the economic efficiency of fish farms.

scholarly journals De novo SCN1A géndeletio terápiarezisztens Dravet-szindrómában

2015 ◽  
Vol 156 (49) ◽  
pp. 2009-2012 ◽  
Author(s):  
Judit Bene ◽  
Kinga Hadzsiev ◽  
Katalin Komlósi ◽  
Erzsébet Kövesdi ◽  
Petra Mátyás ◽  
...  
Keyword(s):  
Copy Number ◽  
De Novo ◽  
Genetic Test ◽  
Molecular Genetic ◽  
Copy Number Variations ◽  
Sequencing Analysis ◽  
Loss Of Function ◽  
Autosomal Dominant Disorder ◽  
Molecular Genetic Test ◽  
Scn1a Gene

Severe myoclonic epilepsy in infancy (Dravet’s syndrome) is a very rare form of epilepsy. Mutations of SCN1A gene encoding voltage-gated sodium channel alpha-1 subunit are major causes of the autosomal dominant disorder. Most cases are associated with a de novo point mutation, but some patients have copy number variations. The protein encoded by the SCN1A gene plays a role in the generation and propagation of action potentials. Loss of function caused by the majority of gene mutations leads to hyperexcitability of the neuronal network that finally results in the formation of the epileptic seizures. Molecular genetic test for copy number variations of SCN1A gene is available in the department of the authors since 2013 besides sequencing analysis of the whole gene. This article presents the case of a 7-year-old patient with two years of recorded patient history outside of the author’s department. Molecular genetic test, which detected a de novo SCN1A gene deletion in heterozygous form, revealed SCN1A gene associated monogenic epileptic syndrome being in the genetic background of therapy-resistant seizures. Orv. Hetil., 2015, 156(49), 2009–2012.

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