scholarly journals Genetic differentiation of black–spotted breed by B–system of blood group

2016 ◽  
Vol 18 (2) ◽  
Author(s):  
A. Zhmur ◽  
V. Bodnaruk
Keyword(s):  
Genetic Variability ◽  
Genetic Differentiation ◽  
Genetic Distance ◽  
Blood Group ◽  
High Frequency ◽  
Holstein Breed ◽  
Statics And Dynamics ◽  
Western Ukraine ◽  
High Level ◽  
Dairy Breed

In animals of western interbreed type allele of B–system: b (0,169), GYE'Q' (0,202), D'G'O' (0,058), G'G'' (0,058), OJ'K'O' (0,054), Q' (0,045) and I2 (0,041) have the highest rate. Proximity of the rate of “Holstein–Frisian” allele GYE'Q' and OJ'K'O'  of Black–Spotted cows indicated interbreed type and Holstein breed is the result of a high level holsteinization of the Western Ukrainian populations. Allele BYA'G'P'Q' G'', GTYB’D’G’Q’Y’B”, YD’G’O’, D’G’O’, G’G” , O’ are typical only Black – Spotted cattle in Western Ukraine and almost never occur in related Black – Spotted breed. The high frequency of allele b in animals of interbreed western type can be explained using sires of Estonian Black – Spotted breed whose frequency but this allele is the highest (0,263). Genetic distance between Western interbreed type, the Ukrainian Black – Spotted Dairy breed and related breeds Black – Spotted breed was: Holstein – 0,483, Western Ukraine – 0,641, Estonian – 0,661, Polish – 0,733, Russian – 0,748 and Lithuanian – 0,830. Herewith the most genetically similar animals were Western interbreed type and Holstein breed. Along with significant commonality of genetic Holstein Western type of Black – Spotted cattle Ukraine has many specific features that are its originality and further confirm the validity of its autonomous existence and improvement. The genes of blood groups reflect changes in the genome populations and cattle breeds and make it possible to determine the features of statics and dynamics of genetic variability.

A Major Disjunction in Genetic Diversity Over the Geographic Range of Acacia melanoxylon R.Br

1993 ◽  
Vol 41 (3) ◽  
pp. 355 ◽  
Author(s):  
J Playford ◽  
JC Bell ◽  
GF Moran
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Genetic Distance ◽  
Allozyme Variation ◽  
Geographic Range ◽  
Considerable Time ◽  
High Genetic Diversity ◽  
Acacia Melanoxylon ◽  
River Region ◽  
High Level

A study of allozyme variation in Acacia melanoxylon R. Br. in 27 populations from across the geographic range of the species indicated high genetic diversity compared to other Australian trees and plants generally. Clines of increasing genetic variability with increasing latitude were found for four measures of genetic diversity. Most of the genetic diversity is found within populations but there was an unusually high level of the variation between populations (37.7%). A distinct genetic separation between the northern and southern populations was located approximately at the Hunter River region, where there is also a disjunction in the distribution of the species. The Nei (1978) genetic distance between these populations within this species is larger than observed between some plant species. Clearly the species has evolved separately in the two regions for a considerable time.

ELECTROPHORETIC VARIATION AND DIFFERENTIATION IN FOUR STRAINS OF DOMESTICATED RAINBOW TROUT (SALMO GAIRDNERI)

1979 ◽  
Vol 21 (1) ◽  
pp. 81-94 ◽  
Author(s):  
Craig A. Busack ◽  
Richard Halliburton ◽  
G. A. E. Gall
Keyword(s):  
Rainbow Trout ◽  
Genetic Variability ◽  
Genetic Differentiation ◽  
Balancing Selection ◽  
Genetic Distances ◽  
Salmo Gairdneri ◽  
Wild Populations ◽  
Electrophoretic Variation ◽  
High Level

A total of 1462 rainbow trout (Salmo gairdneri Richardson) representing four California domestic strains, were examined electrophoretically at 24 gene loci to investigate the amount of genetic variability within strains and genetic differentiation between strains, relative to wild populations. Estimated heterozygosities of 0.071 to 0.134 were similar to those reported for wild populations and thus indicated that the domestic strains were at least as variable as wild populations. Genetic distances between strains ranged from 0.011 to 0.067, values typical of the level of differentiation observed between local conspecific populations in a variety of organisms. It was suggested that this high level of heterozygosity is due to mixing of populations in the strains' histories and perhaps also to balancing selection.

scholarly journals Microsatellite based DNA fingerprinting of 28 local rice (Oryza sativa L.) varieties of Bangladesh

1970 ◽  
Vol 8 (1) ◽  
pp. 7-17 ◽  
Author(s):  
MS Rahman ◽  
MKH Sohag ◽  
L Rahman
Keyword(s):  
Oryza Sativa ◽  
Gene Flow ◽  
Genetic Differentiation ◽  
Genetic Distance ◽  
Dna Fingerprinting ◽  
Oryza Sativa L ◽  
Pcr Amplification ◽  
Rice Varieties ◽  
High Level ◽  
Nei's Genetic Distance

A total of 28 local rice (Oryza sativa L.) varieties of Bangladesh were selected for DNA fingerprinting with seven microsatellite DNA markers. Upon PCR amplification the alleles were separated on Agarose gel using a sequencing gel electrophoresis system. The loci were polymorphic (P95) in all of the varieties. Variation was found in number of alleles, allele frequency, observed and expected heterozygosity. The primer, RM335 having motif (CTT)20 also yielded highest number of alleles (15) and highest PIC value (0.909). Genetic differentiation (Fst) values were found in the ranges 0.84 to 1.00 with an average of 0.92 and gene flow (Nm) values ranged from 0.047 to 0.00 with an average of 0.02. High level genetic differentiation and low level gene flow values in 28 rice (Oryza sativa L.) varieties which were indicated of diversity among the varieties as most of these varieties were of landraces. Over all Nei’s genetic distance value (D) ranged from nil to 2.56 among 378 varietals pairs resulting as a means of permutation combination of 28 rice varieties. The UPGMA dendrograme based on Nei's genetic distance placed the varieties into different clusters. All of the varieties were identified with at least one and/or combination of 7 primers. Keywords: Microsatellites; DNA fingerprinting; Oryza sativa; Bangladesh DOI: 10.3329/jbau.v8i1.6391J. Bangladesh Agril. Univ. 8(1): 7-17, 2010

scholarly journals Genetic diversity and admixture in three native draught horse breeds assessed using microsatellite markers

2018 ◽  
Vol 63 (No. 3) ◽  
pp. 85-93 ◽  
Author(s):  
H. Vostrá-Vydrová ◽  
L. Vostrý ◽  
B. Hofmanová ◽  
N. Moravčíková ◽  
Z. Veselá ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Genetic Differentiation ◽  
Geographical Location ◽  
Membership Probability ◽  
High Genetic Variability ◽  
Individual Level ◽  
Genetic Segregation ◽  
The Individual ◽  
High Level

In this study, we aimed to estimate and compare genetic diversity of two native draught horse breeds and check the possible influence of Noriker breed population on these native breeds. Genetic analyses of relationships and admixture were performed in two native endangered draught horse populations (Silesian Noriker and Czech-Moravian Belgian horses) and one open breed (Noriker). Totally 104 alleles from 13 microsatellite loci were detected in 1298 horses. The average number of alleles per locus was the highest in the Czech-Moravian Belgian horse (7.62) and the lowest in the Silesian Noriker (7.31), the differences were non-significant, whereas the observed and expected heterozygosities per breed ranged from 0.680 (Czech-Moravian Belgian) to 0.719 (Noriker) and from 0.678 (Silesian Noriker) to 0.714 (Noriker). The estimates of Wright’s F<sub>ST</sub> between each pair of breeds indicated a low level of genetic segregation. At the individual level across the analyzed population, formation of two clusters was observed with respect to historical breed development. Moreover, the membership probability outputs showed that the frequencies of alleles varied across the two main regions represented by the Czech-Moravian Belgian and other analyzed breeds. Our results indicated high genetic variability, low inbreeding, and low genetic differentiation, especially between Silesian Noriker and Noriker, which is caused by the high level of admixture. This high level of admixture was in accordance with geographical location, history, and breeding practices of the analyzed breeds. The Silesian Noriker and Noriker breeds seem to be the most genetically related and the decision to consider them as the same population is thus highly supported. The study provides data and information utilizable in the management of conservation programs planned to reduce inbreeding and to minimize loss of genetic variability.  

Population genetics of the hazel hen Bonasa bonasia in Poland assessed with non-invasive samples

2012 ◽  
Vol 7 (4) ◽  
pp. 759-775 ◽  
Author(s):  
Robert Rutkowski ◽  
Marek Keller ◽  
Patrycja Jagołkowska
Keyword(s):  
Population Genetics ◽  
Genetic Variability ◽  
Genetic Differentiation ◽  
Mitochondrial Control Region ◽  
Glacial Refugia ◽  
Genetic Groups ◽  
Bonasa Bonasia ◽  
History Of ◽  
Northeastern Poland ◽  
High Level

AbstractDespite a severe decrease in the number of hazel hens during the 20th century, nowadays this grouse species is rather common in the forests of Northeastern and Southern Poland. We used mitochondrial control region and microsatellite markers to examine the genetic variability of Polish populations of hazel hens. We used non-invasively collected faeces to estimate genetic variability within populations, genetic differentiation among populations as well as genetic differentiation between two regions inhabited by two different subspecies of hazel hens. Our results confirm the usefulness of DNA from faeces to obtain reliable information on the population genetics of hazel hens. We found a rather high level of genetic variability in the Polish population. Genetic variability was higher in birds from continuous forests in the South of the country than in birds from fragmented habitats in the Northeast. Genetic differentiation was higher among subpopulations from Northeastern Poland. Additionally, both classes of molecular markers suggested the presence of two distinct genetic groups of birds, corresponding to previously described subspecies. We conclude that the genetic variability of the Polish hazel hen population has been influenced by habitat fragmentation and the history of the population during its post-glacial colonization of Poland from different glacial refugia.

scholarly journals Genetic variability of broodstocks of Tambaqui (Teleostei – Characidae) from the northeast region of Brazil

2015 ◽  
Vol 36 (6) ◽  
pp. 4013 ◽  
Author(s):  
Nelson Mauricio Lopera-Barrero ◽  
Maria Del Pilar Rodriguez-Rodriguez ◽  
Darci Carlos Fornari ◽  
Emiko Kawakami de Resende ◽  
Angela Rocio Poveda-Parra ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Genetic Differentiation ◽  
Genetic Distance ◽  
Fish Farming ◽  
Population Variability ◽  
Colossoma Macropomum ◽  
Northeast Region ◽  
Shortest Distance

The objective of this experiment was to evaluate the genetic diversity within three Tambaqui broodstocks (Colossoma macropomum). Eight primers were used to analyze 67 individuals collected from three fish farming in the municipalities: Porto Real do Colégio – Alagoas (PRC), Araujo 1 – Sergipe (AR1) and Araujo 2 – Sergipe (AR2), in Brazil. Differences in the frequencies of 88 fragments and four exclusive fragments in PRC were found. High polymorphism values (from 54.38% to 64.38%) and Shannon´s index (from 0.33 to 0.37) were observed. The AMOVA showed that high variation is within each broodstock. The identity and the genetic distance among the groups ranged from 0.845 to 0.975 and from 0.025 to 0.156 respectively, and the shortest distance was found in the groups PRC x AR1 and PRC x AR2. The genetic differentiation ranged from lower to higher (Fst = 0.03 and 0.178) as well as the migratory number per generation (Nm = 5.07 to 12.8). In general, the broodstocks had high intra-population variability, and high differentiation and genetic distance within themselves.

scholarly journals The genesis of the ukrainian red dairy breed

2017 ◽  
Vol 19 (74) ◽  
Author(s):  
N. P. Shevchuk
Keyword(s):  
Dairy Cattle ◽  
Milk Yield ◽  
Milk Fat ◽  
Second Phase ◽  
Holstein Breed ◽  
The Third ◽  
Third Phase ◽  
Increase Milk Production ◽  
High Level ◽  
Dairy Breed

For further improvement of livestock of the Ukrainian Red Dairy Breed it is important to study the genesis because this kind of cow is breed in many farms. Evaluation of the genesis of the breed was done step by step according to the results of crossing red steppe cattle with Angler’s bulls, Red Danish and Holstein breeds. For the research it has been used methods of retrospective analysis and variation statistics. It has been found that the breeding of the Ukrainian Red Diary Breed was done during five stages which have certain characteristics. During the first stage it was carried accumulation of breeding material using Angler breeders in herds of red steppe cattle. During the second phase of creating the breed it has been received a sufficient number of animals with different proportions of blood by Angler and Danish red breeds. According to the standard target average milk yield of cows of the Ukrainian Red dairy cattle is 4000 kg. The specialty of the third phase of breeding was the use of the gene pool of the Holstein breed as the most dairy productive caw. This helped to create intensive Holstein dairy type of the Ukrainian Red dairy breed. The average productivity of firstborn cows with different proportions of blood in Holstein red spoted breed is 5038–5638 kg of milk with fat in it up to 3.78–3.93%. The fourth step is completed with butterfat intrabreed and holshtynised types of Ukrainian dairy cattle and conducted their testing. At the fifth stage the work of heredity consolidation of newly formed breed is held and continues crossbreeding with Holstein breed bulls red spotted colour to increase milk production. Thus, animals of the Ukrainian Red Dairy Breed are characterized by a high level of productivity. At the farm «Colos 2011» in Mykolaiv region the milk yield of cows during 305 days and the third lactation is over 8565 kg of milk, the quantity of milk fat is 332 kg.

Molecular Characterization and Genetic Diversity Study in F3 Population of Rice

2013 ◽  
Vol 20 (1-2) ◽  
pp. 1-8
Author(s):  
MM Rahman ◽  
L Rahman ◽  
SN Begum ◽  
F Nur
Keyword(s):  
Genetic Distance ◽  
Gene Diversity ◽  
Random Amplified Polymorphic Dna ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Polymorphic Loci ◽  
Rice Genotypes ◽  
High Level ◽  
Genetic Diversity Study ◽  
Diversity Study

Random Amplified Polymorphic DNA (RAPD) assay was initiated for molecular genetic analysis among 13 F3 rice lines and their parents. Four out of 15 decamer random primers were used to amplify genomic DNA and the primers yielded a total of 41 RAPD markers of which 37 were considered as polymorphic with a mean of 9.25 bands per primer. The percentage of polymorphic loci was 90.24. The highest percentage of polymorphic loci (14.63) and gene diversity (0.0714) was observed in 05-6 F3 line and the lowest polymorphic loci (0.00) and gene diversity (0.00) was found in 05-12 and 05-15 F3 lines. So, relatively high level of genetic variation was found in 05-6 F3 line and it was genetically more diverse compared to others. The average co-efficient of gene differentiation (GST) and gene flow (Nm) values across all the loci were 0.8689 and 0.0755, respectively. The UPGMA dendrogram based on the Nei’s genetic distance differentiated the rice genotypes into two main clusters: PNR-519, 05-19, 05-14, 05-12 and 05-17 grouped in cluster 1. On the other hand, Baradhan, 05-9, 05-13, 05-11, 05-5, 05-6, 05-1, 05-4, 05-15 and 05-25 were grouped in cluster 2. The highest genetic distance (0.586) was found between 05-4 and 05-17 F3 lines and they remain in different cluster.DOI: http://dx.doi.org/10.3329/pa.v20i1-2.16839 Progress. Agric. 20(1 & 2): 1 – 8, 2009

CONSEQUENCES OF “HOLSTEINIZATION” OF UKRAINIAN BLACK-PIED DAIRY BREED BY GENE BOLA-DRB3.2

2017 ◽  
Vol 54 ◽  
pp. 146-156
Author(s):  
T. M. Suprovych ◽  
M. P. Suprovych ◽  
R. V. Kolinchuk
Keyword(s):  
Genetic Distance ◽  
Milk Production ◽  
Genetic Similarity ◽  
Restriction Analysis ◽  
Allelic Polymorphism ◽  
Milk Productivity ◽  
High Productivity ◽  
Black And White ◽  
Holstein Breed ◽  
Specific Pcr

Introduction. The main direction of increasing the productivity of milk is to increase the proportion of heredity of the Holstein breed in the genotype of cows. Industrial breeds in Ukraine are improving due to the increase in the Holstein inheritance in the genotype of cows. The "holsteinization" of the most widespread domestic Black-and-White diary breed is intensively conducted. Currently, the percentage of heredity from Holstein is 90% or more. The negative effect of "holsteinization" appeared in reducing the resistance of animals to diseases that led to the spread of necrobacterial pathology. The control of the spread of necrobacteriosis can be based on genetic markers. Important markers can be the allele of the BoLA-DRB3.2 gene responsible for the formation of adaptive immunity. Due to the ambiguity of the results of "holsteinization", the following tasks were solved: To study the genetic structure of the herd for the BoLA-DRB3.2 gene at the beginning of the "holsteinization" and now. To compare the detected genetic structures with the alleles spectrum of North American Holstein and identify quantitative and qualitative changes in the structure of the herd genotype. To determine the effect of "holsteinization" on the dynamics of milk production and the state of morbidity by necrobacteriosis. Materials and methods of research. Comparison of alleles of population of the Ukrainian Black-Pied Dairy (UBPD) breed and Holstein breed was conducted to detect the consequences of "holsteinization" on milk yield and incidence of necrobacteriosis. The data of the allelic polymorphism of the BoLA-DRB3.2 gene of the UBPD10 (2010, n = 162), UBPD15 (2015, n = 114) and two Holstein populations of the USA and Canada were collected. The allelic spectrum was determined by the PCR-RFLP method. The amplification of the BoLA-DRB3.2 gene was performed using 2-step PCR with the use of primers HLO-30, HLO-31 and HLO-32 and allele-specific PCR. Restriction analysis was performed with endonuclease RsaI, HaeIII, BstYI (XhoII). Restriction fragments were separated by electrophoresis in 4% agarose gel. Counting of allele frequencies was performed taking into account the number of homozygotes and heterozygotes found for the corresponding alleles. To determine the phylogenetic relationships between the populations of the studied herds, genetic distance and genetic similarity were determined by the M. Nei method. Individual dairy productivity of cows was estimated for all lactation (regardless of its duration). Average milk yields were determined as the total volume of milk produced divided by the number of dairy cows. Results and discussion. The breeding measures carried out led to the accumulation of alleles characteristic of the Holstein breed. For Holstein, there are eight alleles with a frequency of more than 4%. It is alleles *03, *07, *08, *11, *16, *22, *23, *24. A high degree of consolidation of weighty alleles can be outlined. In total they occupy 84,6% of allele spectrum of the population. Consolidation of such alleles in the herd of the Ukrainian Black-and-White diary breed is much lower - only 52.2%, although it increased by 6.2% over 5 years. Alleles *10, *13 and *28 are "weighty" for the Ukrainian Black-and-White diary breed, but they are almost non-existent in Holsteins. The genetic similarity of the herd UBPD15 and Holstein increased by ΔI = 0,085, and the genetic distance between the herds of the UBPD increased by ΔD = 0,085 for 5 years. The comparison of the allele spectrum of Holstein and the Ukrainian Black-and-White diary breed shows both the accumulation and the elimination of alleles associated with high productivity. The largest consolidation is typical for alleles *24 (+ 6.75%) and *16 (+ 4.65%). The frequency of "milk" alleles *22 and *08 decreased, respectively, by 4.14 and 1.27%. Alleys, which cause low milk productivity, have the following dynamics: * 23 + 2.53%, *11 – 0.67 and *28 – 0.26. The accumulation of alleles *16 and *23 (7.18%) was found that are associated with predisposition to necrobacteriosis and elimination of *03 and *22 alleles (4.75%) that influence on this disease. Conclusions. It is determined that the role of alleles characteristic for Holstein is increasing in the the Ukrainian Black-and-White diary herd. Breeding measures for holsteinization are conducted in the right direction. There is accumulation of alleles associated with high milk productivity and predisposition to necrobacteriosis. It positively affects the growth of milk production and negatively affects the incidence of necrobacteriosis.

INFLUENCE OF LIVE WEIGHT IN CULTIVATION ON THE DURATION OF PRODUCTIVE USE OF COWS OF UKRAINIAN BLACK-AND-WHITE DAIRY BREED OF DIFFERENT BLOODLINE ON THE HOLSTEIN BREED

2020 ◽  
Vol 11 (2) ◽  
pp. 16-27
Author(s):  
L. M. Danets ◽  
I. V. Tkachova ◽  
V. P. Shablia
Keyword(s):  
Average Duration ◽  
Live Weight ◽  
Black And White ◽  
Holstein Breed ◽  
Complex Integral ◽  
The Cost ◽  
State Enterprises ◽  
Dairy Breed ◽  
Integral Feature ◽  
A Share

The duration of productive use is a complex integral feature and is determined by both genetic and paratypical factors. This feature should ensure maximum milk productivity of animals, economic efficiency of dairy farms and generally limit the cost of raising and keeping cows. Research conducted in the experimental farms of state enterprises "Kutuzovka", Kharkov district of Kharkov region using cow’s Ukrainian black-and-white dairy breed (4038 cows). We studied the duration of productive use of cows divided into gradations according to the conditional bloodline for the Holstein breed, depending on the weight in the control age periods of cultivation (at birth, at 6, 12 and 18 months). The maximum value of the duration of productive use was recorded in the group of cows with a share of conditional blood for the Holstein breed up to 30% inclusive (the average duration of productive use is 2.77 lactations). The highest indicator of the duration of productive use of the studied animals was 4.09 lactations. Among cows with a share of conditional blood for the Holstein breed up to 30% longer produced those born with a weight of more than 40 kg (4.09 lactations), at 6 months of age weighed 100-149 kg, at 12 months – 200-249 kg, at 18 months of age – 350-399 kg. Among animals with a share of bloodline of 31-60 %, those that had a weight at the age of 6 months produced the longest: 155-190 kg (3.17 lactations), at 12 months – 250-299 kg (2.98 lactations), at 18 months – 350-399 kg (3.06 lactations). In the most numerous gradation with the share of bloodline for the Holstein breed 61-90 %, the longest productive use was characterized by animals born with a weight of 30-39 kg (2.12 lactation), at 6 months of age they weighed more than 200 kg (3.29 lactation), at 12 months-300-349 kg (3.40 lactation), at 18 months – 400-449 kg (2.82 lactation). The average duration of productive use of cows of this grade is quite low – in the range of 2.82-3.29 lactations. The highest degree of influence on the duration of productive use in this gradation of animals was recorded by the live weight factor at 6 months of age (η2 = 10.8). Сcomparative assessment of the cows gradation with a share of conditional blood for the Holstein breed of 91 % or more found that the longest productive use was characterized by cows that had a live weight at birth of 30-39 kg (1.93 lactation), in 6 months – 155-199 kg (2.73 lactation), in 12 months – 250-299 kg (2.87 lactation) and in 18 months – 350-399 kg (2.62 lactation). The degree of influence of live weight at this age on the duration of productive use of cows is insignificant, except for live weight at 6 months of age (η2 = 11.8).

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