scholarly journals Features of experimental modeling of tuberculosis in guinea pig with the participation of N'-(2-(5-((thephylline-7' yl)methyl)-4-R-1,2,4-triazole- ylthio)acethyl)isonicotinohydrazide

2020 ◽  
Vol 10 (4) ◽  
pp. 191-194
Author(s):  
A.S. Gotsulya ◽  
V.V. Zazhzharskiy ◽  
P.O. Davidenko
Keyword(s):  
Guinea Pig ◽  
Microscopic Examination ◽  
Subcutaneous Injection ◽  
Subcutaneous Administration ◽  
Toxic Effects ◽  
Internal Organs ◽  
Single Subcutaneous Injection ◽  
Morphological Abnormalities ◽  
Veterinary Practice ◽  
High Degree

In this study, no toxic effects were detected after single subcutaneous injection of 40 mg/kg tadpoles. The results of macro- and microscopic examination of the internal organs 14 days after single subcutaneous administration of N'-(2-(5-((thephylline-7'-yl)methyl)-4-ethyl-1,2,4-triazole-3-ylthio)acethyl)isonicotino-hydrazide (GKP-305) at a dose of 20, 40 mg/kg showed the absence of any anatomical and morphological abnormalities in the tissue structures of the tentacles. The calculated value of the drug indicates a high degree of safety GKP-305 and its prospects for veterinary practice as an effective and safe tuberculocidal drug.

scholarly journals Histological study of the drug PEG-Filstim sub-acute toxicity

2018 ◽  
pp. 80-88
Author(s):  
V. L. Karbovskyy ◽  
I. A. Shevchuk ◽  
O. V. Kurkina ◽  
T. Ye. Makovska
Keyword(s):  
Defect Formation ◽  
Histological Study ◽  
Subcutaneous Administration ◽  
Toxic Effects ◽  
Internal Organs ◽  
Brain Vessels ◽  
Laboratory Rats ◽  
Applied Dose ◽  
Reticular Tissue ◽  
The Brain

One of the critical steps in development of safe and efficient drugs during their pre-clinical trials are toxicity studies. Therefore, the aim of our work was to study PEG-Filstim toxic effects on animal internal organs and tissues. Toxicity study of PEG-Filstim was performed in 50 white wild-type rats of both sexes with body weight of 170 to 230 g on daily (28 days) subcutaneous administration in the doses of 0.5, 1.0 and 2.0 mg/kg. In all groups of animals, after completing the experiment careful pathomorphologic and histological examination was performed. PEG-Filstim has been shown to possess no toxic effects on internal organs of laboratory rats and does not cause specific changes in the heart, kidneys and mucous coat of stomach on daily subcutaneous administration in the doses of 0.5, 1.0, and 2.0 mg/kg within 28 days. In the maximum applied dose of 2.0 mg/kg, the studied drug causes pronounced acute splenic hyperplasia, related to hyper-proliferation of the reticular tissue, leads to functional strain of the liver due to formation of hematopoietic foci in it, as well as impaired integrity of the respiratory epithelium and congestive signs in the lungs, swelling of the brain tissues, abnormalities in the gray matter structure and hyperemia of the brain vessels. These effects were not observed in the animals, to which the drug was administered in the doses of 0.5 and 1.0 mg/kg. Administration of PEG-Filstim (in all studied doses) results in increasing the size of the ankle joint in rats, which is related to hyper-proliferation of the reticular tissue, leading to bone defect formation in the form of perforation with subsequent filling the periosteum with reticular tissue and formation of hematopoietic foci within its boundaries.

Somatostatin plasma levels and biological effects following subcutaneous administration of somatostatin in man

1986 ◽  
Vol 113 (4) ◽  
pp. 465-470
Author(s):  
L. Uccioli ◽  
G. Ghirlanda ◽  
P. Cotroneo ◽  
A. Manto ◽  
A. Solini ◽  
...  
Keyword(s):  
Plasma Levels ◽  
Subcutaneous Injection ◽  
Bolus Injection ◽  
Biological Effects ◽  
Subcutaneous Administration ◽  
Normal Subjects ◽  
Transient Effect ◽  
Continuous Administration ◽  
Single Subcutaneous Injection ◽  
Insulin Levels

Abstract. The rate at which somatostatin appears in the circulation after subcutaneous bolus injection and continuous administration by pump was determined in normal subjects by serial radioimmunoassays of immunoreactive somatostatin. Following a single subcutaneous injection of 250 μg, the somatostatin peak in plasma appeared after 5 min and had only a transient effect on insulin levels. During continuous administration, somatostatin reached levels able to reduce significantly insulin and glucagon. Somatostatin plasma levels exerting biological effects were observed during the subcutaneous administration of the peptide.

EFFECT OF RELAXIN ON OESTROGEN-INDUCED UTERINE LUMINAL FLUID ACCUMULATION IN THE OVARIECTOMIZED RAT

1974 ◽  
Vol 61 (3) ◽  
pp. 347-353 ◽  
Author(s):  
T. G. KENNEDY
Keyword(s):  
Guinea Pig ◽  
Subcutaneous Injection ◽  
Single Injection ◽  
Daily Treatment ◽  
Ovariectomized Rats ◽  
Ovariectomized Rat ◽  
Fluid Loss ◽  
Fluid Accumulation ◽  
Single Subcutaneous Injection ◽  
Fluid Formation

SUMMARY Uterine luminal fluid accumulation (ULFA) was induced in ovariectomized rats by twice daily treatment for 3 days with 0·5 μg oestradiol-17β. At the end of this treatment, rats were given a single subcutaneous injection of 0·2 ml 0·9% saline containing 0, 0·1, 1, 10, or 100 guinea-pig units (GPU) of relaxin, and ULFA was determined 15–17 h thereafter. It was reduced in animals receiving ≥ 1 GPU relaxin. This reduction in ULFA commenced 12 h after treatment of rats with 10 GPU relaxin, and was completed by 24 h. Ligation of the cervical end of the uterus prevented the loss of fluid in response to a single injection of relaxin, indicating the cervix as the route of fluid loss. Uterine luminal fluid accumulation was reduced to a greater extent by treatment of rats with 1 mg progesterone than by 10 GPU relaxin. Given concomitantly with oestrogen, relaxin had no effect on ULFA in horns which were ligated at the cervical end, indicating that relaxin did not inhibit luminal fluid formation. It is concluded from these results that the effects of progesterone on ULFA are not mediated by relaxin. The results, however, do not allow rejection of the hypothesis that the effect of prolactin on ULFA is mediated by relaxin.

ADVANCEMENT OF OVULATION IN THE GUINEA-PIG WITH EXOGENOUS PROGESTERONE AND RELATED EFFECTS ON LENGTH OF THE OESTROUS CYCLE AND LIFE SPAN OF THE CORPUS LUTEUM

1976 ◽  
Vol 70 (2) ◽  
pp. 275-283 ◽  
Author(s):  
W. D. JOSLYN ◽  
K. WALLEN ◽  
R. W. GOY
Keyword(s):  
Guinea Pig ◽  
Subcutaneous Injection ◽  
Average Length ◽  
Oestrous Cycle ◽  
Corpora Lutea ◽  
Intact Female ◽  
Single Subcutaneous Injection ◽  
Endogenous Oestrogen ◽  
The Mean ◽  
Exogenous Progesterone

SUMMARY A single subcutaneous injection of progesterone (0·4 mg) was given to intact female guinea-pigs on either day 1, 7, 12, 13, 14, 15, or 16 of the behavioural oestrous cycle (day of oestrus is day 0). Injections given on either day 1 or day 7 had little effect, although there was a suggestion that injection on day 7 produced a lengthening of the cycle. Animals injected on either day 12 or day 13 underwent cycles of 16·9 and 18·1 days average length, respectively, which were significantly longer than the mean of 15·8 days for uninjected control females. Injection of progesterone on days 14, 15, or 16 was associated with one of three distinct sequelae: (1) simple prolongation of the cycle associated with a return to spontaneous oestrus 4–7 days later; (2) advancement of ovulation, formation of abnormal corpora lutea, and return to spontaneous oestrus 9–13 days later, and (3) return to spontaneous oestrus 14–16 days after the progesterone injection. These findings suggest that progesterone can cause the release of ovulatory amounts of gonadotrophin following a period of endogenous oestrogen conditioning of the gonadotrophic system. If progesterone is administered before oestrogen conditioning is complete, then it inhibits or delays the conditioning process, and spontaneous oestrus and ovulation are postponed.

Superovulatory response to single subcutaneous injection of Folltropin in Holstein and Sahiwal cows

1992 ◽  
Vol 37 (1) ◽  
pp. 260 ◽  
Author(s):  
A.K. Misra ◽  
S.A. Chaubal ◽  
G. Krishna Kishore ◽  
S. Rajeshwaran ◽  
B.V. Joshi ◽  
...  
Keyword(s):  
Subcutaneous Injection ◽  
Single Subcutaneous Injection ◽  
Sahiwal Cows

HORMONAL REQUIREMENTS FOR THE MAINTENANCE OF OESTRADIOL-INDUCED INHIBITION OF UTERINE SENSITIVITY IN THE OVARIECTOMIZED RAT

1970 ◽  
Vol 46 (3) ◽  
pp. 341-346 ◽  
Author(s):  
K. P. MEYERS
Keyword(s):  
Subcutaneous Injection ◽  
Ovariectomized Rats ◽  
Ovariectomized Rat ◽  
Single Subcutaneous Injection ◽  
The Third ◽  
Oestradiol Treatment ◽  
Progesterone Treatment ◽  
Endometrial Scratching

SUMMARY Ovariectomized rats treated with 2·5 or 5·0 mg. progesterone daily received a single subcutaneous injection of 0·2 μg. oestradiol on the third day of the progesterone treatment. The deciduomal response to trauma by endometrial scratching was used to determine the degree of uterine sensitivity at various times after oestradiol. Uterine sensitivity was partially and then completely lost 36 and 48 hr. after oestradiol administration. The inhibition of uterine sensitivity persisted until 9 and 11 days after oestradiol when the animals received 2·5 and 5·0 mg. progesterone daily. Uterine sensitivity was completely inhibited on day 11 with doses of oestradiol from 0·2 to 0·05 μg. Withdrawal of progesterone treatment for 48 or 72 hr., but not for 24 hr., after oestradiol treatment restored uterine sensitivity. These results show that the oestradiol-induced inhibition of uterine sensitivity in the progestational endometrium is maintained by continuous progesterone treatment and that the duration of this effect is dependent on the dose of progesterone given.

OBSERVATIONS ON CADMIUM DAMAGE AND REPAIR IN RAT TESTES AND THE EFFECTS ON THE PITUITARY GONADOTROPHS

1962 ◽  
Vol 24 (4) ◽  
pp. 453-NP ◽  
Author(s):  
M. ALLANSON ◽  
R. DEANESLY
Keyword(s):  
Subcutaneous Injection ◽  
Cadmium Chloride ◽  
Cytological Study ◽  
Interstitial Cells ◽  
Germinal Epithelium ◽  
Seminiferous Tubules ◽  
Interstitial Tissue ◽  
Long Term Effects ◽  
Single Subcutaneous Injection

SUMMARY Cadmium chloride, in a single subcutaneous injection, can destroy spermatogenic and interstitial cells in the rat testis (Pařízek, 1957) and produce changes in the pituitary. The interstitial tissue is restored by ingrowths from the tunica and full androgen secretion returns before there is any regeneration of germinal epithelium. A cytological study has been made of the peripheral and central pituitary gonadotrophs; the latter revert almost to normal as the interstitial tissue regenerates, whereas the former retain characteristic castration features, unless there is also regeneration of the germinal epithelium. This seems to indicate that in the normal testis there is a hormone contribution from the seminiferous tubules as well as from the interstitial cells. The long-term effects of cadmium on the testis depend on the dose. Early stages of tubule restoration have been studied, but after administration of 0·9 mg., actual proliferation of the germinal epithelium was rarely found—only in four out of twenty rats, 113 or 142 days after injection.

scholarly journals THE TOXIC EFFECTS OF COCAINE PRODUCED BY SUBCUTANEOUS INJECTION.

The Lancet ◽  
1888 ◽  
Vol 131 (3375) ◽  
pp. 872-873 ◽  
Author(s):  
AugustusW. Addinsell
Keyword(s):  
Subcutaneous Injection ◽  
Toxic Effects
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