scholarly journals Qualitative Phytochemical Analysis of Incarvillea emodi (Royle ex Lindl.) Chatterjee and Malva neglecta (Wallr.)

2020 ◽  
Vol 10 (4) ◽  
pp. 93-95
Author(s):  
M. Huzafa ◽  
Z. Jamal ◽  
A. Ahmed
Keyword(s):  
Bioactive Compounds ◽  
Plant Material ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Aerial Parts ◽  
Isolation And Characterization ◽  
Confirmatory Tests ◽  
Phytochemical Profile ◽  
Positive Results

The present study was carried out to investigate the phytochemical profile of Incarvillea emodi and Malva neglecta. The two plants were screened for the presence of nine different classes of bioactive compounds. The qualitative tests were performed on the aerial and underground parts of the selected plants to relate the presence of phytochemicals with bioactivities of the plants. The selected plant material was extracted with three different solvents. These extracts were then subjected to phytochemical screening and the presence of various phytochemicals was confirmed by different standard confirmatory tests. Most of the phytochemicals were found to be present. Results showed that the underground parts of Incarvillea emodi contain comparatively greater number of phytochemicals than the aerial parts. Whereas, in case of Malva neglecta the aerial parts showed more positive results than the underground parts. The current study offers proof for the presence of important bioactive compounds in the studied plants and recommends isolation and characterization of these compounds in the future.

scholarly journals Preliminary phytochemical screening of Iris kashmiriana Baker collected from Budgam, Kashmir, India

2019 ◽  
Vol 9 (1-s) ◽  
pp. 121-124
Author(s):  
Saeema JouFarooqnal ◽  
Roohi Mohi-ud-din ◽  
Zulfiqar Ali Bhat
Keyword(s):  
Secondary Metabolites ◽  
Bioactive Compounds ◽  
Cardiac Glycosides ◽  
Rich Source ◽  
Phytochemical Analysis ◽  
Aqueous Extracts ◽  
Phytochemical Screening ◽  
Isolation And Characterization

The present study was intended to discover the preliminary phytochemicals of Iris kashmiriana Baker collected from Kashmir region, India. The preliminary phytochemical analysis was conducted in methanolic and aqueous extracts which showed the presence of carbohydrates, tannins, flavonoids, phenols, phytosterols, saponins, diterpenes, cardiac glycosides. Among the various phytochemicals studied, alkaloids and proteins were found to be absent in both methanolic and aqueous extracts. From the results, it was noted that the extracts of Iris kashmiriana Baker was found to be a rich source of variety of active secondary metabolites. This report will lead to the further isolation and characterization of these active secondary metabolites for bioefficacy and bioactivity. Keywords: Phytochemical, Bioactive compounds, Iris kashmiriana, Mazar mund, Kashmir.

scholarly journals Preliminary phytochemical profile of Dictyota dichotoma (Huds.) Lamouroux collected from Koothankuzhi Coast, Tirunelveli district, Tamil Nadu, India

2018 ◽  
Vol 8 (6-s) ◽  
pp. 53-56
Author(s):  
Paul J John Peter
Keyword(s):  
Secondary Metabolites ◽  
Tamil Nadu ◽  
Cardiac Glycosides ◽  
East Coast ◽  
Phytochemical Analysis ◽  
Dictyota Dichotoma ◽  
Seaweed Extracts ◽  
Isolation And Characterization ◽  
Phytochemical Profile

The present study was intended to discover the preliminary phytochemicals of Dictyota dichotoma (Huds.) Lamouroux from Koothankuzhi coast, Tirunelveli district, the south east coast of Tamil Nadu, India. The preliminary phytochemical analysis was conducted in seven extracts namely methanol, acetone, chloroform, ethyl acetate, petroleum ether, hexane and benzene by Harborne method. The preliminary phytochemical analysis showed the presence of alkaloids, anthocyanin, anthraquinones, cardiac glycosides, catechin, coumarins, diterpenes, emodins, flavonoids, glycosides, leucoanthocyanin, lignins, phenols, phlobatannins, quinones, saponins, steroids, tannins, terpenoids and triterpenoids. Among the various phytochemicals studied, tannin showed the maximum presence, being found in seven different extracts and anthraquinones was observed in only one extract. From the results, it was noted that the extracts of Dictyota dichotoma (Huds.) Lamouroux was found to be the presence of a number of active secondary metabolites. This report will lead to the isolation and characterization of these active secondary metabolites for bioefficacy and bioactivity. Keywords: Phytochemical, Bioactive compounds, Seaweed extracts, Dictyota, Tamil Nadu.

Phytochemical characterization of Caraway (Carum carvi) seed extract and its use as a potent medicinal agent

2021 ◽  
Vol 16 (10) ◽  
pp. 102-110
Author(s):  
Vivek Chauhan ◽  
Sunita Devi ◽  
Sonaxi Sharma ◽  
Shamsher S. Kanwar
Keyword(s):  
Staphylococcus Epidermidis ◽  
Arable Land ◽  
Seed Extract ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Food Industries ◽  
Carum Carvi ◽  
Medicinal Properties ◽  
The Village

Carum carvi (Caraway) is a member of Apiaceae family which originated in Asia. Due to its economic importance, it is widely cultivated. Caraway is the only annual species, commonly present in arable land, moist meadows and on low lands to mountains. Caraway has different applications in pharmaceutical and food industries. Phytochemical screening of different medicinal plants is helpful in identifying new sources of industrially and therapeutically important compounds. In this study, Caraway obtained from forest surrounding the village Goherman, Lahaul and Spiti, Himachal Pradesh (India) was used. Seed extract of caraway extracted in methanol (MSE) and distilled water (WSE) was used for phytochemical analysis to determine the constituents of caraway seeds. Further, caraway seeds extract was checked for antibacterial activity [Staphylococcus aureus (ATCC 6538), Salmonella typhimurium (NCTC 74), Escherichia coli (ATCC 25922) and Staphylococcus epidermidis (ATCC 12228)], antioxidant activity and acid neutralizing activity. After analysing the results, it can be said that caraway seeds possess many medicinal properties and it can be used in enhancing human health.

scholarly journals Prevalence of Vibrio alginolyticus in Sediment Samples of River and Coastal Areas of Bangladesh

2016 ◽  
Vol 29 (1) ◽  
pp. 1-6 ◽  
Author(s):  
R Siddiqui ◽  
MM Alam ◽  
MN Naser ◽  
Y Otomo ◽  
M Yasmin ◽  
...  
Keyword(s):  
Fish Population ◽  
Vibrio Alginolyticus ◽  
Live Cells ◽  
Economic Damage ◽  
Biochemical Tests ◽  
Marine Animals ◽  
Isolation And Characterization ◽  
Vibrio Pathogens ◽  
Positive Results

Vibrio alginolyticus has been thought to be a halophilic marine bacterium that causes diarrhea, otitis media and wound infection through the consumption of raw or inappropriately cooked sea food. It is one of the main Vibrio pathogens affecting marine animals, such as marine fish, shrimp and shellfish which lead to large economic damage. Although there are reports on the presence of this organism in the coastal area of other countries, not so much work has been done on the isolation and characterization of this species in Bangladesh. The present study was, therefore, undertaken to isolate and characterize V. alginolyticus organisms isolated from the rivers (fresh water) and estuaries (brackish water) of Bangladesh. A total of 9 isolates of Vibrio species were obtained from different water bodies (three from Meghna river, two from Shangu river and four from estuary) and provisionally identified as Vibrio alginolyticus following standard biochemical tests. All these 9 strains showed same pattern of antibiotic resistance to ampicillin, streotomycin, penicillin, but sensitive to nalidixic acid. In the virulence properties test, two isolates showed positive results for toxR gene and none of the isolates showed positive results for tdh gene. Challenge experiments in Singhi fish (Heteropneustes fossi) with the live cells and the culture filtrate prepared from the V. alginolyticus showed high mortality of the fish population. All these studies suggest the presence of pathogenic V. alginolyticus strains in the river water and estuarine bodies of Bangladesh and the extracellular toxin(s) of the V. alginolyticus might be one of the causes for fish mortality.Bangladesh J Microbiol, Volume 29, Number 1, June 2012, pp 1-6

scholarly journals Lilium Philadelphicum Flower as a Novel Source of Antimicrobial Agents: A Study of Bioactivity, Phytochemical Analysis and Partial Identification of Antimicrobial Metabolites

2021 ◽  
Author(s):  
Shefali Singh ◽  
Vineeta Singh ◽  
Alaa Alhazami ◽  
B.N. Mishra ◽  
Shafiul Haque ◽  
...  
Keyword(s):  
Cell Viability ◽  
Bioactive Compounds ◽  
Bioactive Compound ◽  
Biologically Active ◽  
Gas Chromatography Mass Spectrometry ◽  
Achromobacter Xylosoxidans ◽  
Phytochemical Analysis ◽  
Antimicrobial Potential ◽  
Compound Concentration

The members of the Liliaceae family have been regarded as an excellent source of biologically active compounds. However, the work on antimicrobial potential and characterization of the bioactive fractions of Lilium philadelphicum flower is limited and needs to be explored. The present study reports the antimicrobial potential, anti-inflammatory and anticancer potential of the bioactive fraction extracted from the flower of L. philadelphicum (Red Lily) and characterization of these bioactive compounds. The antimicrobial activity was tested against nine different Gram-positive and Gram-negative bacterial strains. The minimum inhibitory concentration (MIC) values of methanolic extract of L. philadelphicum flower against Acinetobacter bouvetii, Achromobacter xylosoxidans, Bacillus subtilis MTCC 121, Candida albicans MTCC 183, Klebsiella pneumoniae MTCC 3384, and Salmonella typhi MTCC 537 were 25, 50, 12.5, 50, 100 and 50 μg mL-1, respectively. The phytochemical analysis of the extract reveals the presence of phenols, flavonoids, tannins, terpenoids, glycosides, coumarins, and quinones. The cytotoxicity of the partially purified compound against the HepG2 cell line in MTT assay demonstrates up to 90% cell viability with a bioactive compound concentration of 50 μg/ml. However, with the increase in bioactive compound concentration up to 1000 μg/ml results into nearly 80% cell viability, just a minor decline in cell viability suggests the importance of bioactive compounds for suitable therapeutic applications. Spectroscopic studies of the bioactive compound by UV-Visible spectroscopy, FT-Infra Red spectroscopy, Gas Chromatography-Mass Spectrometry (GCMS) as well as its phytochemical analysis suggests the presence of terpenoids moiety, responsible for the antimicrobial property of L. philadelphicum flower.

scholarly journals An Overview of Methods of Extraction, Isolation and Characterization of Natural Medicinal Plant Products in Improved Traditional Medicine Research

2020 ◽  
pp. 31-57
Author(s):  
Dobgima John Fonmboh ◽  
Ejoh Richard Abah ◽  
Tembe Estella Fokunang ◽  
Bayaga Herve ◽  
Gerald Ngo Teke ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Traditional Medicine ◽  
Chemical Diversity ◽  
Bioactive Metabolites ◽  
Plant Biodiversity ◽  
Phytochemical Screening ◽  
Herbal Products ◽  
Isolation And Characterization ◽  
Herbal Plants

Herbal plants are very important in traditional community use and enrich our plant biodiversity and conservation. Natural products are vital substances of traditional knowledge systems in complementary and alternative medicine, nutraceutical, food supplements, and pharmaceutical bioactive metabolites of new chemical entities. Bioactive secondary metabolites from herbal plants of different forms are main sources and provide major opportunities for drug active pharmaceuticals due to the diverse flora and fauna biodiversity that produces the necessary available chemical diversity. There has been an increasing popularity in phytochemical research within the high through put (HTS) screening programs in search of lead. Phytochemicals of herbal extracts for traditional uses contain various types of bioactive metabolites of pharmaceutical and pharmacotherapeutic nature, and many phytomedicines for different therapeutic areas have been derived from herbal products. This paper is aimed at giving an insight into the extraction, isolation, and characterization of the rich medicinal plant biodiversity of potential pharmaceutical importance and the major drawbacks and challenges in the extraction, isolation, and characterization of phytochemicals in plant extracts. Phytochemicals in medicinal plants have been studied with more emphasis on the extraction process which is a vital stage in the analysis of bioactive compounds in medicinal plant research. The advantages and disadvantages of the different extraction methods is important to discuss following the regulatory guidelines and different pharmacopoeia. The analysis of bioactive molecules in herbal products involves the applications of various phytochemical screening methods, and the use of chromatographic techniques such as TLC and HPLC, including in some cases the non-chromatographic methods like Fourier Transform Infra-Red (FTIR), immunoassay. This paper has been motivated by the challenges faced by most pharmacy students in data mining of information on phytochemical screening and testing of biological activities in projects related to herbal plants research. This write up is also geared towards providing students with information on the preclinical drug discovery process towards the formulation of an improved traditional medicine/ phytomedicine.

scholarly journals Molecular characterization of Nocardiopsis species from Didwana dry salt lake of Rajasthan, India

2021 ◽  
Vol 13 (1) ◽  
pp. 396-401
Author(s):  
Khushbu Parihar ◽  
Alkesh Tak ◽  
Praveen Gehlot ◽  
Rakesh Pathak ◽  
Sunil Kumar Singh
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Bioactive Compounds ◽  
Salt Lake ◽  
Bioactive Compound ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Multiple Sequence ◽  
Isolation And Characterization

The genus Nocardiopsis is well known to produce secondary metabolites especially antibacterial bioactive compound. Isolation and characterization of bioactive compounds producing novel isolates from unusual habitats are crucial. The present study was aimed to explore Didwana dry salt lake of Rajasthan state in India for the isolation and characterization of actinomycetes. The isolated actinomycetes isolates were characterized based on culture characteristics, biochemical tests and 16S rRNA gene sequencing. The 16S rRNA gene sequence analysis revealed that all the five isolates inhabiting soil of the said dry salt lake of Didwana, Rajasthan belonged to four species of Nocardiopsis viz., N. synnemataformans, N. potens, N. prasina and N. dassonvillei subsp. albirubida. The molecular identification based on 16S rRNA gene sequences was found accurate and robust. The phylogram generated through multiple sequence alignment of all the test isolates of Nocardiopsis revealed that the isolates aroused from a single branch and validated monophyletic association. The present study is the first report of exploring Nocardiopsis isolates from the dry salt lake. These characterized Nocardiopsis isolates isolated from Didwana dry salt lake habitat are novel stains and can be of significance in the detection and utilization of novel bioactive compounds.

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