scholarly journals РОЗРОБКА СПОСОБІВ ОТРИМАННЯ І АНАЛІЗ ФЕРМЕНТНИХ ПРЕПАРАТІВ ПЕРОКСИДАЗ ТА КАТАЛАЗ ДЕЯКИХ ВИДІВ БАЗИДІОМІЦЕТІВ

2013 ◽  
Vol 3 (01) ◽  
pp. 113 ◽  
Author(s):  
O. V. Fedotov ◽  
T. E. Voloshko
Keyword(s):  
Freeze Drying ◽  
Enzyme Preparation ◽  
Gel Filtration ◽  
Science Research ◽  
Individual Characteristics ◽  
Salting Out ◽  
Enzyme Preparations ◽  
Agrocybe Cylindracea ◽  
Activity Of Enzymes ◽  
The Individual

<p>A method for obtaining of enzyme preparations of enzyme preparations (EP) of peroxidases and catalases fungal extracellular and inracellular origin from cultures of Basidiomycetes was developed. The strains <em>Flammulina velutipes</em> F-vv, <em>Agrocybe cylindracea</em> 167; <em>Fistulina hepatica</em> Fh-08 and <em>Pleurotus ostreatus</em> P-208 and P-01 were used as producers of oxidoreductases. Strains were grown on modified glucose-peptone media. Fractionation was carried out by salting out the enzymes with ammonium sulfate at 40-70% saturation of peroxidases and 80% of saturation - for catalase. These solutions protein fractions was further purified by dialysis and gel filtration on Molselekt granules G-50 and G-75. The enzyme solution was subjected to freeze-drying. The individual characteristics of the enzyme preparations were found.  The individual characteristics of the enzyme preparations are the activity of enzymes, the protein content and amino-acid composition of enzyme preparations. It was established that strain <em>F. velutipes</em> F-vv was an active producer of intracellular and strain of <em>A. cylindracea</em> 167 was an active producer of extracellular peroxidase. The strains of <em>P. ostreatus</em> P-01 and P-208 were the active producers of extracellular catalase, and the strains of F. hepatica Fh-08 were active producers of intracellular catalase. The developed methods for producing of enzymes catalase and peroxidase preparations of extra-and intracellular origin provided new antioxidant enzymes, which have their own properties and application prospects in various sectors of industry and science research.</p> <p><em>Key words: Basidiomycetes, peroxidases, catalases, enzyme preparation.</em></p>

scholarly journals Modification of isolation methods and physico-biochemical properties of preparations of fungal oxidoreductases

2020 ◽  
Vol 11 (2) ◽  
pp. 310-314
Author(s):  
O. V. Fedotov ◽  
Z. L. Usikova
Keyword(s):  
Amino Acids ◽  
Lentinula Edodes ◽  
Gel Filtration ◽  
Amino Acid Content ◽  
Toxicity Testing ◽  
Culture Fluid ◽  
Biochemical Properties ◽  
Individual Characteristics ◽  
Intracellular Location ◽  
Enzyme Preparations

The results of the modification of methods for producing enzymatic preparations (EP) of peroxidases and catalases of extra- and intracellular finding from fungal cultures are presented. Strains of Flammulina velutipes F-vv, Lentinula edodes 523 and Pleurotus ostreatus P-01 were used as producers of oxidoreductases. The producers were cultured in glucose-peptone medium, modified for each strain. Protein fractionation was carried out by leaching with ammonium sulfate at a saturation of 40–70% for peroxidases and 80% for catalases. The obtained solutions of protein fractions were additionally subjected to purification by dialysis, gel filtration on Molselect G-50 and G-75 granules, and also freeze drying. The yield of enzymatic preparations per unit mass of mycelium and the volume of culture fluid were calculated. The individual characteristics of EP – enzymatic activity, the mass percentage of protein and associated amino acids, the ratio of the latter in groups depending on the nature of the radicals (amphotericity) of protein molecules are established. It was proved that the amino acid content in the proteins of fungal EP catalases and peroxidases indicates their acidic nature and this is confirmed by the pH values of aqueous solutions. Examination and toxicity testing of enzymatic preparations were carried out in certified laboratories, which confirmed their characteristics and compliance with safety requirements. The therapeutic properties of amino acids that are part of proteins or are in a free state in enzyme preparations are analyzed. In this way, the methods have been developed for producing enzymatic preparations of peroxidases and catalases of extra- and intracellular location, which allow new antioxidant enzymes with individual properties to be obtained, and, as a result, bring prospects for use in various industries and scientific research.

scholarly journals A study of calf thymus histone fraction F2(b) by gel filtration

1970 ◽  
Vol 120 (1) ◽  
pp. 61-66 ◽  
Author(s):  
P. A. Edwards ◽  
K. V. Shooter
Keyword(s):  
Sodium Chloride ◽  
Salt Concentration ◽  
Spherical Symmetry ◽  
Gel Filtration ◽  
Histone Fraction ◽  
Salting Out ◽  
Hydrodynamic Volume ◽  
Calf Thymus ◽  
Stokes Radius ◽  
The Individual

The gel-filtration behaviour of calf thymus histone fraction F2(b) was studied at three different salt concentrations (0.01m-, 0.10m- and 1.00m-sodium chloride) and two different pH ranges (pH3–4 and pH6.7–7.1). Other histone fractions [F1, F2(a) and F3] were also utilized to assist interpretation of the data. It was found that the Stokes radius of histone fraction F2(b) was not significantly changed when the salt concentration was increased, implying that the aggregation of the individual histone molecules (Edwards & Shooter, 1969) resulted in only relatively minor changes in the hydrodynamic volume. Aggregation would appear to be due to the salting out of hydrophobic regions giving rise, in the aggregate, to a compact core of hydrophobic groups from which protrude the remaining basic parts of the molecule. Repulsion between charged groups on the basic regions of individual histone molecules would give the aggregate approximately spherical symmetry, the diameter of the aggregate approximating to the length of a single histone molecule.

scholarly journals Secondary exocrine pancreatic insufficiency. Features of the choice of the enzyme

2021 ◽  
pp. 192-199
Author(s):  
I. G. Pakhomova
Keyword(s):  
Clinical Practice ◽  
Enzyme Preparation ◽  
Pancreatic Insufficiency ◽  
Clinical Manifestations ◽  
Exocrine Pancreatic Insufficiency ◽  
Individual Characteristics ◽  
Negative Consequences ◽  
Exocrine Insufficiency ◽  
Enzyme Replacement ◽  
The Individual

Exocrine pancreatic insufficiency is quite common in clinical practice of doctors of many specialties. Decrease in intraduodenal lipase levels below 5–10% of normal leads to pancreatic steatorrhea, weight loss and a potential decrease in quality of life. These clinical manifestations are more often presented in patients with a primary genesis of exocrine pancreatic insufficiency, while secondary exocrine insufficiency is more often subclinical, which predetermines its early diagnosis. However, many methods for diagnosing exocrine pancreatic insufficiency, which have been developed and used in clinical practice in recent years, have low sensitivity or specificity, or are time-consuming and poorly tolerated by patients. The method for determining the level of coprological elastase is not always reliable, especially in patients with chronic diarrhea, which predetermines its implementation in formalized stool, for example, during therapy. It is important to take into account the fact that with moderate exocrine pancreatic insufficiency, the sensitivity and specificity of assessing the level of fecal elastase can weaken by 30%. However, regardless of the receipt of diagnostic data and the cause of the development of exocrine pancreatic insufficiency, the goal of therapy is to eliminate the processes of maldigestion and malabsorption, reduce the severity of symptoms of indigestion and prevent their negative consequences. At the  same time, it is necessary to include an enzyme preparation in the complex therapy, the choice of which, its dosage and duration of therapy are determined taking into account the individual characteristics of the patient and the severity of exocrine pancreatic insufficiency. In addition, in patients with suspected exocrine pancreatic insufficiency due to clinical manifestations, it is possible to use empirical enzyme replacement therapy. An example of such an approach for patients with secondary exocrine insufficiency is the appointment of the enzyme preparation containing pancreatin.

scholarly journals Modification of isolation methods and physico-biochemical properties of preparations of fungal oxidoreductases

2020 ◽  
Vol 11 (2) ◽  
pp. 310-314
Author(s):  
O. V. Fedotov ◽  
Z. L. Usikova
Keyword(s):  
Amino Acids ◽  
Lentinula Edodes ◽  
Gel Filtration ◽  
Amino Acid Content ◽  
Toxicity Testing ◽  
Culture Fluid ◽  
Biochemical Properties ◽  
Individual Characteristics ◽  
Intracellular Location ◽  
Enzyme Preparations

The results of the modification of methods for producing enzymatic preparations (EP) of peroxidases and catalases of extra- and intracellular finding from fungal cultures are presented. Strains of Flammulina velutipes F-vv, Lentinula edodes 523 and Pleurotus ostreatus P-01 were used as producers of oxidoreductases. The producers were cultured in glucose-peptone medium, modified for each strain. Protein fractionation was carried out by leaching with ammonium sulfate at a saturation of 40–70% for peroxidases and 80% for catalases. The obtained solutions of protein fractions were additionally subjected to purification by dialysis, gel filtration on Molselect G-50 and G-75 granules, and also freeze drying. The yield of enzymatic preparations per unit mass of mycelium and the volume of culture fluid were calculated. The individual characteristics of EP – enzymatic activity, the mass percentage of protein and associated amino acids, the ratio of the latter in groups depending on the nature of the radicals (amphotericity) of protein molecules are established. It was proved that the amino acid content in the proteins of fungal EP catalases and peroxidases indicates their acidic nature and this is confirmed by the pH values of aqueous solutions. Examination and toxicity testing of enzymatic preparations were carried out in certified laboratories, which confirmed their characteristics and compliance with safety requirements. The therapeutic properties of amino acids that are part of proteins or are in a free state in enzyme preparations are analyzed. In this way, the methods have been developed for producing enzymatic preparations of peroxidases and catalases of extra- and intracellular location, which allow new antioxidant enzymes with individual properties to be obtained, and, as a result, bring prospects for use in various industries and scientific research.

THE METHODS OF DEVELOPING COMPUTATIONAL THINKING OF STUDENTS WHILE STUDYING THE COURSE "NUMERICAL METHODS" BASED ON BLENDED LEARNING

2019 ◽  
pp. 34-41
Author(s):  
M. M. Klunnikova
Keyword(s):  
Numerical Methods ◽  
Blended Learning ◽  
Computational Thinking ◽  
Individual Characteristics ◽  
Diagnostic Model ◽  
Mathematics Students ◽  
Professional Activities ◽  
Cognitive Component ◽  
Professional Field ◽  
The Individual

The work is devoted to the consideration of improving the quality of teaching students the discipline “Numerical methods” through the development of the cognitive component of computational thinking based on blended learning. The article presents a methodology for the formation of computational thinking of mathematics students, based on the visualization of algorithmic design schemes and the activation of the cognitive independence of students. The characteristic of computational thinking is given, the content and structure of computational thinking are shown. It is argued that a student with such a mind is able to manifest himself in his professional field in the best possible way. The results of the application of the technique are described. To determine the level of development of the cognitive component of computational thinking, a diagnostic model has been developed based on measuring the content, operational and motivational components. It is shown that the proposed method of developing computational thinking of students, taking into account the individual characteristics of students’ thinking, meaningfully based on the theoretical and practical aspects of studying the discipline, increases the effectiveness of learning the course “Numerical methods”. The materials of the article are of practical value for teachers of mathematical disciplines who use information and telecommunication technologies in their professional activities.

MEDIA SOCIALIZATION OF PERSONALITY IN THE CONTEXT OF INMUTATION IN THE MASS MEDIA

2021 ◽  
pp. 104-109
Author(s):  
Chernysh O.O.
Keyword(s):  
Mass Media ◽  
Negative Impact ◽  
Effective Interaction ◽  
Individual Characteristics ◽  
Media Culture ◽  
Active Growth ◽  
Media Space ◽  
The Media ◽  
The Individual

The urgency of the researched problem is connected with the growing role of mass media in modern conditions leads to change of values and transformation of identity of the person. The active growth of the role of the media, their influence on the formation and development of personality leads to the concept of “media socialization” and immutation in the media. The aim of the study is to outline the possibilities of the process of media socialization in the context of immutation in the media. The methods of our research are: analysis of pedagogical, psychological, literature, synthesis, comparison, generalization. The article analyzes the views of domestic and foreign scientists on the problem of immutation in the media and the transformation of the information space. In the context of the mass nature of the immutation of society, the concept of “media socialization” becomes relevant, which is the basis for reducing the negative impact of the media on the individual.The author identifies the lack of a thorough study of the concept of “media socialization” in modern scientific thought. Thus, media socialization is associated with the transformation of traditional means of socialization, and is to assimilate and reproduce the social experience of mankind with the help of new media.The article analyzes the essence of the concepts “media space”, “mass media” and “immutation”. The influence of mass media on the formation and development of the modern personality is described in detail.The study concluded that it is necessary to form a media culture of the individual, to establish safe and effective interaction of young people with the modern media system, the formation of media awareness, media literacy and media competence in accordance with age and individual characteristics for successful media socialization. The role of state bodies in solving the problem of media socialization of the individual was also determined. It is determined that the process of formation of media culture in youth should take place at the level of traditional institutions of socialization of the individual.The author sees the prospect of further research in a detailed analysis and study of the potential of educational institutions as an institution and a means of counteracting the mass nature of the immutation of society.Key words: immutation, media socialization, mass media, media space, information.

DECISION-MAKING ON INVESTMENT OF IT-INNOVATION BASED ON FUZZY EXPERT INFORMATION

2018 ◽  
pp. 103-111
Author(s):  
Olga Olegovna Eremenko ◽  
Lyubov Borisovna Aminul ◽  
Elena Vitalievna Chertina
Keyword(s):  
Decision Making ◽  
Individual Characteristics ◽  
Production Model ◽  
Investment Management ◽  
It Projects ◽  
Product Model ◽  
Project Cost ◽  
Reasoning System ◽  
Managerial Decisions ◽  
The Individual

The subject of the research is the process of making managerial decisions for innovative IT projects investing. The paper focuses on the new approach to decision making on investing innovative IT projects using expert survey in a fuzzy reasoning system. As input information, expert estimates of projects have been aggregated into six indicators having a linguistic description of the individual characteristics of the project type "high", "medium", and "low". The task of decision making investing has been formalized and the term-set of the output variable Des has been defined: to invest 50-75% of the project cost; to invest 20-50% of the project cost; to invest 10-20% of the project cost; to send the project for revision; to turn down investing project. The fuzzy product model of making investment management decisions has been developed; it adequately describes the process of investment management. The expediency of using constructed production model on a practical example is shown.

THEORETICAL PRINCIPLES OF FORECASTING THE COST OF A SOFTWARE PRODUCT

2017 ◽  
Author(s):  
Pavlo Rodionov ◽  
◽  
Anna Ploskonos ◽  
Lesya Gavrutenko ◽  
◽  
...  
Keyword(s):  
Selection Process ◽  
Individual Characteristics ◽  
Successful Implementation ◽  
Software Products ◽  
Software Product ◽  
Continue Research ◽  
Cocomo Model ◽  
Factors Of Influence ◽  
The Individual ◽  
The Cost

The paper analyzes the factors that affect the amount of effort required to create a mobile application and its cost. It is established that the main factors of influence are the design of the application, its functionality, the type of mobile platform, the availability and level of testing and support, as well as the individual characteristics of the developer. Based on the analysis of information sources, the main methods and approaches to forecasting the cost of software products are identified, which include the COCOMO model, Price-to-win method, expert evaluation, algorithmic methods and the method of analogies. It is proposed to consider the method of analogies as a tool that allows you to make predictions about the cost of resources required for the successful implementation of IT projects based on the experience of similar projects. It is proved that the advantages of this method are the simplicity of its implementation and the clarity of the results obtained, which follows from the practical orientation of this tool. Among the limitations of the method of analogy is the mandatory need for reliable data relating to similar projects, as well as the difficulty of taking into account unspecified indicators. Taking into account the mentioned limitations of the method of analogies and on the basis of the analysis of scientific sources the possible directions of its optimization are determined. Thus, among the ways to improve the effectiveness of this method are those aimed at optimizing the project selection process, the data for which are used as a basis for forecasting. Attempts to improve the method of analogies by including parameters that were previously ignored by this technique seem promising. This in turn can lead to an expansion of the scope of the method of analogies and increase the accuracy of forecasts. As prospects for further research, the need to continue research in the field of optimization of the method of analogies with the subsequent practical verification of theoretical positions on the data of real projects.

Methods for assessing territory pollution by oil or petroleum product spills

2020 ◽  
pp. 140-146
Author(s):  
S. I. Chelombitko ◽  
V. V. Piven
Keyword(s):  
Oil And Gas ◽  
Radiant Energy ◽  
Petroleum Products ◽  
Individual Characteristics ◽  
Oil And Gas Fields ◽  
Northern Regions ◽  
Negative Impacts ◽  
The Individual ◽  
Destructive Processes ◽  
Gas Fields

During the development of oil and gas fields and transportation of hydrocarbons, the ecology of regions is subject to various negative impacts. The most severe consequences for the environment are caused by accidental spills of oil or petroleum products, which occur due to through damage to pipelines. Polluted territories are subject to mandatory reclamation in accordance with government regulations and industry regulations. However, despite the measures taken by the administrations of the northern regions to increase the rate of reclamation of damaged territories in recent years, there has been a trend of growth of areas contaminated with hydrocarbons.Various remediation technologies that have been tested in warm and temperate climates are not very effective in regions with permafrost. Pollution of the daytime surface with oil or petroleum products leads to an increase in the absorption of the sun's radiant energy, the growth of the seasonal thaw layer and the development of destructive processes for the territory. To achieve a positive effect as a result of rehabilitation measures, it is necessary to take into account the individual characteristics of the territory and the extent of its pollution.

Effect of Visual Feedback on the Static and Kinematic Characteristics of Handwriting

2017 ◽  
Vol 27 ◽  
pp. 5-21 ◽  
Author(s):  
Michael Pertsinakis
Keyword(s):  
Visual Feedback ◽  
Educational Level ◽  
Movement Analysis ◽  
Individual Characteristics ◽  
Software Module ◽  
Medium Education ◽  
Education Levels ◽  
Pen Tablet ◽  
The Individual ◽  
Document Examiners

Research on visual feedback has not produced consistent results to show how visual feedback or the lack, thereof, influences individual handwriting characteristics. A two-pronged approach was designed to investigate the degree of this influence. For this purpose, samples of signatures as well as cursive and block text, written with and without visual feedback, were collected from 40 volunteers and imported into a PC via a pen tablet, using an electronic inking pen. The data was analyzed in a handwriting movement analysis software module specially designed for this research that was added to the software MovAlyzeR by Neuroscript LLC. Two forensic document examiners (FDEs) independently analyzed samples from the two groups (samples executed with normal visual feedback versus the group of samples executed without visual feedback). They found no fundamental differences between these two groups. Their analyses also demonstrated that a large number of similarities existed in the general design of the allographs (alternative forms of a letter or other grapheme) and in the pictorial aspects, regardless of the complexity of the samples. In the cursive and block handwriting, four main qualitative characteristics were linked to the absence of visual feedback: change of overall size, non-uniformity of left margins, change of baseline alignment, and inclusion of extra trajectories. The statistical analysis verified the above findings. The comparative analysis also suggests that gender, educational level (above high school) and handedness create an insignificant influence on the individual characteristics of writing produced with and without visual feedback. The only notable exception is the relationship between signature duration and educational level. The volunteers with a medium education level showed a significant increase in duration while signing their names without visual feedback in comparison to those with higher education levels. The combination of the above findings suggests that handwriting is not fundamentally influenced by visual feedback.  Purchase Article - $10

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