scholarly journals Effect of consumption of I, Se, S and nanoaquacitrates on hematological and biochemical parameters of the organism of rabbits

2021 ◽  
Vol 12 (2) ◽  
pp. 335-340
Author(s):  
О. V. Boiko ◽  
О. F. Honchar ◽  
Y. V. Lesyk ◽  
І. І. Kovalchuk ◽  
B. V. Gutyj ◽  
...  
Keyword(s):  
Blood Cells ◽  
White Blood Cells ◽  
Reproductive Function ◽  
Hemoglobin Concentration ◽  
Control Group ◽  
Limiting Factor ◽  
Group Iii ◽  
Mineral Substances ◽  
Preparation Period ◽  
Experimental Group

Using nanotechnologies to obtain compounds of mineral substances is promising because of the importance of the amount of mineral substances, but the limiting factor is particularly their bioavailability in the digestive tract. The conducted studies determined the stimulating effect of nanoaquachelates of separate biogene microelements on the course of biochemical processes of the organisms of animals, their productivity and quality of the obtained products. However, the mechanisms of impact of carboxylates of separate elements and their combinations on functioning of physiological systems of various species of animals, level of transformation of these elements into the products and their biological value remain unstudied. Therefore, the objectives of the study were changes in hematological biochemical and productive parameters of the organisms of rabbits consuming I, Se, S, the impacts of these elements on their organism from the 53 to 85th days of life. Studies were performed on young Termonde rabbits. Animals of experimental groups I, II, III, and IV were fed with feeds of the diet of the control group for 24 h with water containing solution of І, Se, S calculated respectively as 2.5, 5.0, 10.0 and 20.0 µg of І/L of water. The experiment lasted 45 days, including a 12 day preparation period and 33 day experimental. On the 53rd day of life of animals in the preparation period, and 68th and 85th days of life in the experimental periods (15th and 33rd days of watering with supplements), we collected samples of blood from the marginal ear vein of 6 animals (3 males and 3 females) of the group for hematological and biochemical studies. We determined that watering with the solution of microelements І, Se, S led to changes in the overall amount of white blood cells, making it 17.1% lower in the blood of animals of the experimental group II, 26.2% higher in group ІІІ on the 15th day, and 12.0% higher in group IV on the 31st day of the experiment compared with the control. Absolute amounts of lymphocytes, monocytes and granulocytes by the periods of the study were observed to have no significant changes throughout the study, though their parameters were within the physiological values compared with the control. The amount of red blood cells in the blood of rabbits of experimental groups І, ІІ and ІІІ was higher respectively by 8.1%, 5.7% and 12.0% at the first stage of the study and 18.1%, 13.3%, 24.5% and 24.9% higher in animals of experimental groups І, ІІ; ІІІ and ІV at the final stage of the study compared with the control group. Hemoglobin concentration in blood of rabbits of experimental groups II and III during the study was significantly higher. Hematocrit value in blood of rabbits of experimental groups I–III was significantly higher on the 33rd day of the experiment compared with the control. Consumption of solution of microelements in the amount of 10.0 µg of I/L by the animals of experimental group III led to 15.1% higher content of total protein in the blood on the 33rd day of the study compared with the control group of animals. The level of creatinine was no higher than the physiological parameters and was 9.2%, 15.0% and 15.4% higher in the blood of rabbits of experimental groups II, III, IV on the 33rd day of the experiment compared with the control group. No significant changes in the content of triacylglycerols in the blood of rabbits of the experimental groups were seen throughout the study. However, the content of cholesterol in the blood of rabbits of experimental groups III and IV was significantly higher at the first stage compared with the control group. Further, it is practical to study physiologically substantiated amounts of solution of microelements І, Se, S in the diet of mother rabbits to determine their effect on their ability to become pregnant, reproductive function, milk production and survival of offspring.

Effect of miR-155 on Aplastic Anemia Rats Through Regulating Signal Transducer and Activator of Transcription 3 Signaling Pathway

2020 ◽  
Vol 10 (3) ◽  
pp. 397-401
Author(s):  
Qingxian Yan ◽  
Zhengjun Hou ◽  
Shuting Ye ◽  
Meiyun Su
Keyword(s):  
Aplastic Anemia ◽  
Signaling Pathway ◽  
Blood Cells ◽  
Mrna Level ◽  
White Blood Cells ◽  
Control Group ◽  
Stat3 Signaling ◽  
Group I ◽  
Stat3 Signaling Pathway ◽  
Experimental Group

Objective: To assess miR-155’s effect on aplastic anemia (AA) rats. Methods: In the present study, the healthy rats (control group) and AA rats including AA rats with miR-155 overexpression (experimental group I) and those with miR-155 deficiency (experimental group II), were selected. The levels of miR-155, STAT3 (a key gene in STAT3 signaling pathway) and phosphorylated STAT3 (p-STAT3) in control group and experimental group were detected via qPCR and Western blotting. Moreover, the number of white blood cells (WBCs), red blood cells (RBCs), platelets (PLTs) and hemoglobin (HGB) level were also measured. Results: The level of miR-155 in AA rats was significantly declined compared with that in healthy rats (p < 0.05). STAT3 mRNA level was significantly declined in AA rats with miR-155 overexpression compared with that in AA rats with miR-155 deficiency (p < 0.05). STAT3 and p-STAT3 protein expression in AA rats with miR-155 overexpression were significantly lower than those in AA rats with miR-155 deficiency (p < 0.05). Besides, it was found that the number of WBC, RBC, PLT and HGB level were significantly elevated in AA rats with miR-155 overexpression compared to those in AA rats with miR-155 deficiency (p < 0.05). Conclusion: miR-155 can improve the AA symptoms of rats through inhibiting the STAT3 signaling pathway.

scholarly journals Tartrazine induces structural and functional aberrations and genotoxic effectsin vivo

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3041 ◽  
Author(s):  
Latifa Khayyat ◽  
Amina Essawy ◽  
Jehan Sorour ◽  
Ahmed Soffar
Keyword(s):  
Comet Assay ◽  
Blood Cells ◽  
White Blood Cells ◽  
Pharmaceutical Products ◽  
Food Additive ◽  
Control Group ◽  
Adverse Health Effects ◽  
Male Wistar Rats ◽  
Liver And Kidney ◽  
Experimental Group

Tartrazine is a synthetic organic azo dye widely used in food and pharmaceutical products. The current study aimed to evaluate the possible adverse effect of this coloring food additive on renal and hepatic structures and functions. Also, the genotoxic potential of tartrazine on white blood cells was investigated using comet assay. Twenty adult male Wistar rats were grouped into two groups of 10 each, control- and tartrazine-treated groups. The control group was administered orally with water alone. The experimental group was administered orally with tartrazine (7.5 mg/kg, b.wt.). Our results showed a marked increase in the levels of ALT, AST, ALP, urea, uric acid, creatinine, MDA and NO, and a decreased level of total antioxidants in the serum of rats dosed with tartrazine compared to controls. On the other hand, administration of tartrazine was associated with severe histopathological and cellular alterations of rat liver and kidney tissues and induced DNA damage in leucocytes as detected by comet assay. Taken together, the results showed that tartrazine intake may lead to adverse health effects.

scholarly journals Hematological Importance of Pseudoeosinophilic Granulocytes in Acclimation of Common Carp (Cyprinus Carpio Linnaeus, 1758)

2017 ◽  
Vol 40 (1) ◽  
pp. 5-11
Author(s):  
Damir Suljević ◽  
Adelaida Martinović-Jukić ◽  
Muhamed Fočak ◽  
Andi Alijagić ◽  
Dunja Rukavina ◽  
...  
Keyword(s):  
Thermal Stress ◽  
Water Temperature ◽  
Cyprinus Carpio ◽  
Blood Cells ◽  
White Blood Cells ◽  
Control Group ◽  
Adaptation Mechanisms ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio ◽  
Experimental Group

Abstract Adaptation mechanisms as response to water content, oxygen level and pollutants are very important and they can be interpreted by hematological analysis. The aim of this study was the analysis of hematological and immune adaptations of common carp (Cyprinus carpio Linnaeus, 1758) to thermal stress. All specimens were divided into a control and experimental group. The control group of fish was exposed to a constant water temperature of 10°C. We induced thermal stress in experimental fish by gradually heating water to 28°C, held for 30 minutes and then comparing the obtained results with the control fish. Short-term hyperthermia lead to an increase of the number of leukocytes, especially pseudoeosinophilic granulocytes and monocytes, while the number of neutrophils and lymphocytes was reduced. The analysis of the leukocyte number and differential blood count in the control group showed high individual variation of segmented granulocytes, monocytes and pseudoeosinophilic granulocytes. Statistically significant differences (p=0.00) were found for the white blood cells, nonsegmented neutrophils and pseudoeosinophils between the control and experimental group. The experimental group of males had an increased number of white blood cells, monocytes and pseudoeosinophils, where significant differences were found for nonsegmented and total neutrophils and also for pseudoeosinophils (p=0.00), lymphocytes (p=0.01) and monocytes (p=0.03). Females had an increased total number of white blood cells, lymphocytes, monocytes and pseudoeosinophils, while significant differences (p=0.00) were obtained in the number of white blood cells, nonsegmented and total neutrophils and pseudoeosinophils between the control and experimental group. Adaptation mechanisms in carp after water temperature heating are mostly reflected in the increase of pseudoeosinophils and the decrease of neutrophils.

scholarly journals Effect of ketorolac administration post bone cavitation surgery on some hematological parameters

2017 ◽  
Vol 41 (1) ◽  
pp. 169-177
Author(s):  
Raffal A. Omar
Keyword(s):  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Treated Group ◽  
Control Group ◽  
Mean Corpuscular Hemoglobin ◽  
Mean Corpuscular Hemoglobin Concentration ◽  
Corpuscular Hemoglobin ◽  
Post Operation

     This study was designed to determine the effect of ketorolac on some hematological parameters post cavitation surgery in domestic rabbits. Ninety-six adult domestic local breed male rabbits were used in this study, weighing (1.23 ± 0.33) kg. They were divided randomly into two groups in the first one (n= 48/group) which represented control group, while the second one (n=48/group) represented the treated group (Ketorolac group). All rabbits in both groups undergoes surgical operation (bone cavitation in femur bone) under general anesthesia. In the Ketorolac group, animals were treated by using 30 mg/kg of Ketorolac directly post-operation and continued daily for 5 days, while in Control group no treatment was given postoperatively. The hematological parameters were recorded which include white blood cells count; red blood cells count; hemoglobin; packed cell volume; mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration; mean corpuscular volume and platelets at the period of (3, 5, 7, 14, 21, 28, 35, and 42) days post operation were estimated. Results concerning hematological values showed no significant differences in packed cell volume values between control and treated group and within each group, while haemoglobin values showed a significant decrease at period 7 and 14 days between control and treated group. Within control group the significant decrease were noticed clearly at period of 7 days. Data concerning mean corpuscular hemoglobin values within control group showed significant decrease were noticed at 5 day, but within treated group, there were no significant differences. mean corpuscular hemoglobin concentration values showed significant decrease ate period 3; 5 and 21 days between control group and treated group respectively. Within control group the significant decrease were noticed obviously at 5 days while within treated group the significant decrease were noticed at 35 days. mean corpuscular volume values showed significant increase at 3 and 5 days period between control and treated groups. Platelets counts within control group showed significant increase at period of 14 days, within treated group the significant increase were recorded at 42 days. Red blood cells showed significant decrease between control and treated groups, while within control group, the significant decrease were at 7 days. White blood cells count within treated group showed significant increase recorded at 21 days. Differential White blood cells count concerning Lymphocytes cells within control group the significant increase were recorded at 42 days  while the significant decrease were noticed at 21 days, but within treated group the significant increase were recorded at 35 days  and significant decrease were noticed at 14 days. Neutrophils counts showed significant decrease at periods 3, 14, 28 and 42 days between control and treated groups respectively; while within control group there were significant decrease at 3 days, but within treated group the significant decrease were noticed at 42 days. Monocytes cells showed significant increase at 35 days between control and treated groups, within control group there were significant increase at 35 days, while within treated group the significant increase recorded at 7 days.

scholarly journals Haematological and Genotoxic effects of cadmium chloride on Mesopotamichthys sharpeyi

2016 ◽  
Vol 13 (4) ◽  
pp. 745-752
Author(s):  
Baghdad Science Journal
Keyword(s):  
Red Blood Cells ◽  
Cell Volume ◽  
Packed Cell Volume ◽  
Blood Cells ◽  
Cadmium Chloride ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Control Group ◽  
Blood Picture ◽  
Genotoxic Effects

The present study investigated Haematological changes in Mesopotamichthys sharpeyi, as well as determination genotoxic effects of cadmium chloride on bunni fish by using 120 fingerlings, fish were distributed randomly into four treatments in addition to control group. Fish in first group treated (T1) with cadmium 0.093mg/L with changing water and added cadmium continuously, fish in the second group treated (T2) with cadmium 0.093mg/L with changing water without adding cadmium, third treatment (T3) with cadmium 0.046mg/L with changing water and adding cadmium continuously, and fourth treatment (T4) with cadmium 0.046mg/L with changing water without adding cadmium. Results of blood picture in T1 and T3 showed a significant reduction in red blood cells count, hemoglobin concentration, packed cell volume values, while the number of white blood cells showed a significant increase in its values. Results showed presence of improvement of clinical and microscopical signs and blood picture in T2 and T4, were changed water aquarium continuously and added cadmium only once compared withT1 and T3. Results of the present study concluded that changing water aquarium in the treatments without adding cadmium led to improvement of health status of fish which increased with the passage of time results of blood picture were almost the same of the control group. It could be concluded from the current study that the adding of cadmium to water aquarium containing bunni fish led to decrease in red blood cells count, hemoglobin and packed cell volume values and increase in micronuclei number.

HEMATOLOGICAL INDICATORS OF CALVES IN THE PREVENTION OF HYPOELEMENTOSIS IN THE AMUR REGION

2020 ◽  
pp. 148-153
Author(s):  
N. N. Malkova ◽  
◽  
М. Е. Ostyakova ◽  
S.А. Sherbinina ◽  
◽  
...  
Keyword(s):  
Blood Cells ◽  
Color Index ◽  
White Blood Cells ◽  
Experimental Period ◽  
Amur Region ◽  
Hemoglobin Level ◽  
Blood Oxygenation ◽  
Control Group ◽  
Hematological Indicators ◽  
Experimental Group

Hematological studies were conducted in calves in dynamics during the prophylaxis of hypo-elementosis in the Amur region. The object of the study was calves (breed-Holstein, age-up to two months). The selected animals were divided into two equivalent groups: control and experimental. In the control group, planned measures were taken to prevent hypo - elementosis; in the experimental group, complex injectable drugs were used: Se-containing and tissue. Laboratory tests of calves ‘ blood were performed at the beginning and end of the experiment with determining the number of red blood cells, white blood cells, hemoglobin level, calculating the color index, differential counting of white blood cells and calculating the color index. It was established that the quality of blood oxygenation in calves of the experimental group improved. This is confirmed by the regeration to the standard values of hemoglobin level and color index as a result of their growth by 28 and 17%, respectively. At the end of the experimental period, the percentage of conditionally healthy calves was 80 % in the experimental group and 40 % in the control group. Thus, it should be noted that the proposed scheme of complex application of Se-containing and tissue preparations contributed to the normalization of erythropoiesis processes and increased the resistance of calves to diseases by 40 %.

Study The Effect of Dietary Supplementation of Crushed Seeds of Coriander (Coriandrum Sativum) on Some Physiological Parameters of Rabbits.

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Sura Safi Obayes Khafaji
Keyword(s):  
Total Protein ◽  
Blood Cells ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Basal Diet ◽  
Experimental Period ◽  
Physiological Parameters ◽  
Coriandrum Sativum ◽  
Control Group ◽  
T1 And T2

The aim of present research to study the effects of crushed seeds of Coriander (Coriandrum sativum) on some physiological parameters in rabbits. Thirty mature male rabbits, (aged 87-100 days),were divided randomly into three equal groups. First group (control) fed the basal diet along the experimental period,2nd and 3rd groups (T1 and T2) fed the basal diet mixed with 3% and 6%,respectively. At the end of experiment,blood samples were collected from ear vein for counting the red blood cells (RBCs) and white blood cells (WBCs),estimating Hemoglobin concentration (Hb) and packed cell volume (PVC). Total protein,albumin,globulin,blood glucose,GOT and GPT concentrations were estimated in blood serum. The results showed significant increase (P<0.05) of RBCs,WBCs,Hb and PCV levels in T1 and T2 groups when compared with control group. As well as,The results of total protein and globulin increased significantly in two treated groups in comparison with control groups. While,the results of glucose,GOT and GPT levels showed a significant decrement (P<0.05) in T1 and T2 groups compared with control group. Therefore,it was concluded that the use of Coriander seeds powder can affected positively on some physiological traits in rabbits.

scholarly journals Effect of alcoholic extract of Apium graveolens leaves on some physiological properties of a broilers

2014 ◽  
Vol 13 (1) ◽  
pp. 111
Author(s):  
S. A. Al – Gnami
Keyword(s):  
Treatment Group ◽  
Blood Cells ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Apium Graveolens ◽  
Control Group ◽  
Distilled Water ◽  
Alcoholic Extract ◽  
Physiological Properties ◽  
Before And After

This study was carried out on fourteen broilers arranged between (1100 – 1500) gm. in weight to estimate the effect of drenching of alcoholic extract of Apium graveolens leaves on some physiological properties. The birds were divided into two groups which are control and treatment group seven birds for each group. The birds in both groups are housed in the same condition, include temperature, light, food, and water during the experiment period. The control group savaged with 1 ml of distilled water while treatment group savaged with 10 mg\kg. B.W. of alcoholic extract of Apium graveolens leaves dissolved in 1 ml of distilled water for two weeks. The birds were weighed and a blood sample was taken two times before and after treatment. The results of this study were revealed a significant increase in red blood cells count, hemoglobin concentration, and packed cell volume, while there is no significant changes in white blood cells count, and body weight.

scholarly journals The toxic effects of ammonia on growth rate and some blood parameters in common carp (Cyprinus carpio L.)

2018 ◽  
Vol 11 (1) ◽  
pp. 109-113
Author(s):  
Q Jumaa ◽  
K Al-Saeedy ◽  
W Obid
Keyword(s):  
Treatment Group ◽  
Common Carp ◽  
Blood Cells ◽  
Negative Impact ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Blood Parameters ◽  
Control Group ◽  
Common Carp Cyprinus Carpio ◽  
Fish Breeding

This study was performed to know the effect of ammonia on the blood picture and the weight of common carp fish. 50 fish were used at the age of 6 months and were divided into two groups. Each group included 25 fish. The first group represented the treatment group with ammonia at a concentration of 1.0 mg/ L. and the second group represented control group. It is grown in plastic basins with dimensions of 1.5 × 2 meters and continued treatment for four weeks. It was observed that the weight in the ammonium treatment group had the lowest significant value (p≤0.05) compared to the control group. It was also observed in this study that the ammonia group had the lowest significant values in (white blood cells, red blood cells, hemoglobin concentration and Packed cell volume) compared with control group. As for the biochemical parameters, it was observed that the Glucose concentration and each of the enzymes Alanine Aminotransferase (ALT) and Aspartate Aminotransferase (AST) had a record the highest value in the group treated with ammonia compared with the control group while the total protein recorded the lowest significant value in the group treated with ammonia Compared to control group. We conclude from this study that increasing the concentration of ammonia in the aquatic environment has a negative impact on fish breeding and health.

scholarly journals The effect of the microelement preparation on the morphobiochemical composition of the blood of calves

2021 ◽  
Vol 22 (3) ◽  
pp. 418-427
Author(s):  
S. V. Nikolaev
Keyword(s):  
Blood Cells ◽  
Saline Solution ◽  
White Blood Cells ◽  
Control Group ◽  
Intact Group ◽  
Stable Maintenance ◽  
Positive Effect ◽  
Experimental Group ◽  
Time Injection ◽  
Milk Feeding

The paper presents an assessment of the effect of the Sedimine microelement product on the morphobiochemical composition of the blood of calves during the milk feeding period. For the experiment, 2 groups of heifers of HolsteinKholmogory breed at the age of 2-3 weeks were formed. The calves of the experimental group were injected intramuscularly with Sedimine at a dose of 5 ml three times with an interval of 7 days, and the control animals were given a saline solution in the same way. Blood for hematological and biochemical studies was obtained at the beginning of the experiment and a week after the first and last injection. According to the research it has been established, that after a single injection of Sedimine, there were no evident changes in the biochemical profile of the blood, with the exception of a decrease in the level of urea by 30.6% (from 4.9 to 3.4 mmol/l; P<0.001). After three-time administration of the drug, there was an increase in total protein by 9.6 % (from 54.2 to 59.4 g / l; P<0.05) and an increase in AlAT activity by 1.2 times (P<0.001), while in comparison with the control, the activity of AsAT was greater (P<0.01) by 43.4 % (57.5 U/l). The de Ritis coefficient in the experimental group decreased by 43.1 % (to 6.2; P<0.01), while in the control group, on the contrary, the indicator increased by 32.1 % (to 11.1). The ratio of calcium to phosphorus in all animals decreased by 28.8...32.1 % (P<0.001), but in young animals treated with Sedimine, the ratio was 16.7 % (0.84) higher compared to the intact group of calves (P<0.05). The concentration of thyroglobulin in the experimental group significantly decreased by 29.4 % (from 0.34 to 0.24; P<0.01), with stable values in calves that were treated with saline. Injections of the preparation contributed to the stable maintenance of iron in the blood of animals of the experimental group, the concentration of which was 3 times higher (P<0.01) in relation to the control, where the indicator was 5.9 micromol/l. Also, the control animals showed a decrease in the number of red blood cells by 31.9% (from 4.7 * 10 12 to 3.2*10 12) and hemoglobin by 19.5% (from 87.2 to 70.2 g / l; P<0.05), with stable values in the experimental group, which were higher by 56.3 % (5.0*1012) and 22.2 % (90.2 g/l) compared to the control (P<0.05). Changes in white blood were characterized by a higher level of white blood cells in calves of the experimental group (by 27.7 %), primarily due to a 36.7 % (4.1*109 ; Р<0.01) higher number of lymphocytic cells. Thus, a three-time injection of the test drug was quite effective in preventing iron deficiency in calves during milk feeding and had a positive effect on metabolic processes.

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