scholarly journals Antioxidant protection enzyme activity in the blood serum and large intestinal mucosa of rats with prolonged gastric hypochlorhydria and given multiprobiotics

2019 ◽  
Vol 10 (4) ◽  
pp. 438-444
Author(s):  
S. V. Pylypenko ◽  
A. A. Koval
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Blood Serum ◽  
Glutathione Reductase ◽  
Catalase Activity ◽  
Glutathione Transferase ◽  
Reduced Glutathione ◽  
Control Group ◽  
Antioxidant Protection ◽  
Colon Mucosa

The activity of antioxidant protection enzymes in the blood serum and colon mucosa in rats was studied under the conditions of 28-days administration of omeprazole on its own and omeprazole together with multiprobiotics "Symbiter" and "Apibact". Physiological and biochemical study methods were applied. It was found that after omeprazole administration, the activity of superoxide dismutase in the blood serum decreased, and the activity of catalase increased compared to the control. With the co-administration of omeprazole and multiprobiotics, the activity of superoxide dismutase increased compared to the group of rats that received omeprazole only during the same time, but remained less compared to the control group. The content of reduced glutathione in the blood serum of rats after administration of omeprazole decreased, the activity of glutathione peroxidase and glutathione transferase increased, and the activity of glutathione reductase decreased compared to the control. With co-administration of omeprazole and multiprobiotics, the serum RG content was at the control level, the activity of glutathione reductase exceeded the control values. The activity of glutathione reductase decreased compared to the group receiving omeprazole only. The activity of glutathione reductase increased and did not differ from the control values. In the colon mucosa, superoxide dismutase and catalase activity decreased compared to control. With the combined administration of omeprazole and multiprobiotics, superoxide dismutase and catalase activity increased and even exceeded the control values. With the administration of omeprazole, the reduced glutathione content in the colon mucosa was lower than that in the control. The activity of glutathione peroxidase increased and glutathione transferase and activity of glutathione reductase decreased compared to the control. With co-administration of omeprazole and multiprobiotics to rats, the reduced glutathione content increased compared to the group of rats administered omeprazole only, and even exceeded that in the control.

scholarly journals Biochemical changes in rat muscle tissue with prolonged use of simvastatin

2018 ◽  
Vol 99 (2) ◽  
pp. 240-244
Author(s):  
E V Vinogradova
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Muscle Tissue ◽  
Pyruvic Acid ◽  
Comparison Group ◽  
Reduced Glutathione ◽  
Antioxidant Protection ◽  
Titin Isoforms ◽  
Rat Muscle

Aim. Analysis of biochemical changes in rat muscle tissue after prolonged use of simvastatin. Methods. The study was conducted on mongrel male rats. Three groups were identified: control group (intact animals), comparison group (animals with induced hypercholesterolemia not reeciving the drugs), and experimental group (animals with induced hypercholesterolemia receiving simvastatin 0.0012 g/100 g of weight once a day for 2 months as an aqueous suspension through the esophageal probe). Metabolite concentration of glycolysis (pyruvic acid and lactate), activity of antioxidant protection enzymes (reduced glutathione, superoxide dismutase, catalase, glutathione reductase, glutathione peroxidase), titin isoforms and proteolytic fragments of titin and nebulin concentration were determined in the muscles of animals. Results. After administration of simvastatin to animals with induced hypercholesterolemia, a decrease in the concentration of glycolysis metabolites (pyruvic acid and lactate) compared to comparison group was revealed, as well as multidirectional changes in the activity of antioxidant protection enzymes (decrease in activity of superoxide dismutase, glutathione peroxidase, and glutathione reductase, decreased concentration of reduced glutathione, but catalase activity remained unchanged). The analysis of structural changes in animal muscle tissue after administration of simvastatin revealed a decrease in the concentration of NT- and N2A-titin isoforms and practically complete absence of nebulin compared to the animals from the comparison group. At the same time an increase in the concentration of proteolytic fragments of titin (T2) by 1.3 times was recorded. Conclusion. The study showed that the basis of myotoxicity of statins in their long-term use is disintegration of enzyme antioxidant processes, as well as tissue hypoxia, leading to destruction of muscle fibers and prevalence of proteolytic processes.

State of the enzyme link of antioxidant protection in boys with hypoandrogenism

2020 ◽  
Vol 40 (4) ◽  
pp. 32-36
Author(s):  
L. K. Parkhomenko ◽  
◽  
L. A. Strashok ◽  
S. I. Turchina ◽  
G. V. Kosovtsova ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Free Radical ◽  
Glutathione Peroxidase ◽  
Free Radical Oxidation ◽  
Conjugated Dienes ◽  
Delayed Puberty ◽  
Control Group ◽  
Antioxidant Protection ◽  
Radical Oxidation

Recently, interest in the problem of free radical oxidation in biological membranes, which is directly related to both the normal functioning of cells and the occurrence, course and outcome of many pathological conditions, has increased again in clinical medicine. The aim was to determine the role and impact of antioxidant defense in boys with hypoandrogenism. The study involved 75 adolescents with hypoandrogenism aged 13–18 years, who underwent a complex of clinical and laboratory examinations. All patients were conducted complex of anthropometric research and determination of the degree of delayed puberty, laboratory and instrumental examination. Free radical oxidation was determined by the levels of malondialdehyde, conjugated dienes, carbonated proteins, superoxide dismutase and catalase in the serum, and restored glutathione and glutathione peroxidase in whole blood. Based on their determination, the coefficient of oxidative stress was calculated. Statistical processing of results was performed using parametric and nonparametric methods. The study of indicators of the free radical oxidation process found that adolescents with hypoandrogenism have multidirectional changes in the oxidation of proteins and lipids, namely: the level of conjugated dienes increases, the concentration of malondialdehyde remains at the level of the control group, and the level of carbonated proteins tends to decrease. As for the activity of antioxidant protection enzymes, a significant decrease in the level of glutathione peroxidase was detected, while the level of superoxide dismutase and catalase remained at the level of normative indicators. Oxidative stress accompanies and is one of the pathogenetic links in the formation or maintenance of the state of hypoandrogenism in boys. This requires the use of antioxidants, the complex of which must be selected individually.

Glutathione and glutathione-related enzymes in rats exposed to dimethoate and/or pyrantel

2014 ◽  
Vol 17 (1) ◽  
pp. 105-112 ◽  
Author(s):  
A. Spodniewska ◽  
D. Barski ◽  
H. Ziółkowski
Keyword(s):  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Reduced Glutathione ◽  
Oxidative Activity ◽  
Antioxidative System ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Combined Administration ◽  
Applied Dose

AbstractThe study was undertaken to examine the effect of single and combined administration of dimethoate (an OP insecticide) and pyrantel embonate (an anthelmintic agent) on the concentration of reduced glutathione (GSH) and the activity of glutathione peroxidase (GPx) and glutathione reductase (GR) in rats. Dimethoate (Group I) was administered to rats at a dose of 1/10 LD50for 5 consecutive days and pyrantel embonate (Group II) at a dose of 1/5 LD50for 3 consecutive days. The animals of group III were given both of the mentioned above compounds in the same manner as group I and II, but pyrantel embonate was applied on day 3, 4, and 5 from the beginning of dimethoate intoxication. Material from 6 rats randomly selected from each group was obtained after 3, 6 and 12 hours and 2, 7 and 14 days following the last applied dose of the compounds under study. It was found that application of pyrantel embonate caused only slight changes in the analysed parameters i.e. GSH, GPx and GR. Dimethoate administration caused disturbances in the antioxidative system manifested as a decrease in GSH concentration in the liver (max. - 37.7% after 6 hours) and an increase of GPx and GR activities in erythrocytes (max. - 21.7% and 29.6% after 3 hours, respectively), compared to the control group. The profile of changes after combined intoxication was similar, but their intensity was higher compared to the group of animals exposed to dimethoate only. Based on current studies, it was concluded that both dimethoate and pyrantel embonate at the applied doses showed a pro-oxidative activity.

Dietary supplementation with magnesium citrate may improve pancreatic metabolic indices in an alloxan-induced diabetes rat model

10.5219/1375 ◽  
2020 ◽  
Vol 14 ◽  
pp. 836-846
Author(s):  
Olena Shatynska ◽  
Oleksandr Tokarskyy ◽  
Petro Lykhatskyi ◽  
Olha Yaremchuk ◽  
Iryna Bandas ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Fasting Glucose ◽  
Reduced Glutathione ◽  
Pancreatic Tissue ◽  
Magnesium Supplementation ◽  
Metabolism Enzymes ◽  
Peroxidation Index

The purpose of the current study was to evaluate the protective properties of dietary magnesium supplementation on pancreatic tissue of rats with alloxan-induced diabetes mellitus. Twenty-five male Wistar rats were split into five groups (control, diabetes, diabetes with 100 mg Mg daily, diabetes with 250 mg Mg daily, diabetes with 500 mg Mg daily) with feeding supplementation starting on day 1, diabetes induction on day 21, and animal sacrifice on day 30. Fasting glucose in blood serum was measured on days 21, 25, 27, and day 30. Glucose metabolism enzymes, namely, lactate dehydrogenase and glucose-6-phosphate dehydrogenase, were measured in pancreatic tissue upon the sacrifice, as well as lipid peroxidation, antioxidant system protective enzymes (catalase and superoxide dismutase), and glutathione system components (glutathione reductase, glutathione peroxidase, and glutathione reduced). Pearson correlation coefficients showed strong negative correlation between serum glucose (control and diabetic animals) and glucose metabolism enzymes, catalase, superoxide dismutase, glutathione peroxidase in pancreatic tissue (r >-0.9, p <0.05), moderate negative correlation with reduced glutathione (r = -0.79, p <0.05), moderate positive correlation with lipid peroxidation index (r = +0.67, p <0.05), weak correlation with glutathione reductase (r = -0.57, p <0.05). Magnesium supplementation slowed down diabetes onset considering fasting glucose levels in rats (p <0.05), as well as partially restored investigated dehydrogenase levels in the pancreas of rats comparing to diabetes group (p <0.05). The lipid peroxidation index varied between treatments showing the dose-dependent influence of Mg2+. Magnesium supplementation partially restored catalase and superoxide dismutase activities in pancreatic tissue, as well as glutathione peroxidase and reduced glutathione levels (p <0.05), while glutathione reductase levels remained unaffected (p >0.05). The obtained results suggested a model, where magnesium ions may have a possible protective effect on pancreatic tissue against the negative influence of alloxan inside β cells of the pancreas.

scholarly journals In VivoAntioxidant Activity of Deacetylasperulosidic Acid in Noni

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
De-Lu Ma ◽  
Mai Chen ◽  
Chen X. Su ◽  
Brett J. West
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Catalase Activity ◽  
Antioxidant Properties ◽  
Superoxide Dismutase Activity ◽  
Morinda Citrifolia ◽  
Control Group ◽  
Dose Dependent

Deacetylasperulosidic acid (DAA) is a major phytochemical constituent ofMorinda citrifolia(noni) fruit. Noni juice has demonstrated antioxidant activityin vivoand in human trials. To evaluate the role of DAA in this antioxidant activity, Wistar rats were fed 0 (control group), 15, 30, or 60 mg/kg body weight per day for 7 days. Afterwards, serum malondialdehyde concentration and superoxide dismutase and glutathione peroxidase activities were measured and compared among groups. A dose-dependent reduction in malondialdehyde was evident as well as a dose-dependent increase in superoxide dismutase activity. DAA ingestion did not influence serum glutathione peroxidase activity. These results suggest that DAA contributes to the antioxidant activity of noni juice by increasing superoxide dismutase activity. The fact that malondialdehyde concentrations declined with increased DAA dose, despite the lack of glutathione peroxidase-inducing activity, suggests that DAA may also increase catalase activity. It has been previously reported that noni juice increases catalase activityin vivobut additional research is required to confirm the effect of DAA on catalase. Even so, the current findings do explain a possible mechanism of action for the antioxidant properties of noni juice that have been observed in human clinical trials.

scholarly journals ANTIOXIDANT PROTECTION SYSTEM DURING CHRONIC INFLAMATION OF THE CONNECTIVE TISSUE IN COWS

2012 ◽  
Vol 10 (3) ◽  
pp. 53-54
Author(s):  
Konstantin Aleksandrovich Nadein
Keyword(s):  
Superoxide Dismutase ◽  
Connective Tissue ◽  
Glutathione Reductase ◽  
Chronic Inflammation ◽  
Ankle Joint ◽  
Protection System ◽  
Control Group ◽  
Clinical Test ◽  
Antioxidant Protection ◽  
Antioxidant Protection System

As clinical test material we studied the blood serum taken from clinically healthy animals (the control group) and animals with chronic inflammation of the connective tissue in an ankle joint (the experimental group). To study the antioxidant protection system enzyme component condition in cows we examined the following: catalase and glutathione peroxidase activity (as a substrate we used hydrogen dioxide and glutathione reductase), ferroxidase activity of ceruloplasmin, superoxide dismutase activity. A spectrocolorimeter «Specol 210» was used to determine the solutions absorbancy. The results shows the breakdown of membrane-bounded enzymes activity (such as glutathione reductase and superoxide dismutase). These enzymes characterize the antioxidant protection system condition. It is essential to examine the antioxidant protection system condition in animals with chronic inflammation of the connective tissue in an ankle joint to enrich our knowledge about the mechanism of metabolic disorder at this type of pathology, as well as to treat and correct possible complications.

scholarly journals The activity of the antioxidant protection enzymatic system of boars with a decrease in their reproductive capacity under oxidative stress

2020 ◽  
Vol 8 (3) ◽  
pp. 194-197
Author(s):  
V. I. Koshevoy ◽  
S. V. Naumenko
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Antioxidant Defense ◽  
Control Group ◽  
Enzymatic System ◽  
Antioxidant Protection ◽  
Reproductive Ability ◽  
Experimental Group

It was found that enzymatic system activity of the antioxidant protection of the breeding boars organism with a decrease in their reproductive ability under oxidative stress was significantly decreased, which was noted by a decrease in the antioxidant capacity. Males of the experimental group had a decrease in superoxide dismutase activity by 30,2% (p ˂ 0,01). At the same time, in the erythrocytes of breeding boars, catalase activity was reduced by 33,9% (p ˂ 0,001) compared with the indicators of the control group animals, due to the high sensitivity to nitric oxide, the content of which was significantly increased during oxidative stress; interacting with nitric oxide, a suppressed form of catalase – ferricatalase-NO was formed. A decrease in the activity of these enzymes is characteristic of the oxidative stress development – which is due to the high level of peroxidation processes in the body of breeding boars. With a decrease in the activity of glutathione system components, an imbalance of the prooxidant-antioxidant system was noted. According to the research results, a decrease in the activity of the glutathione redox cycle enzymes – glutathione peroxidase and glutathione reductase was noted, which in boars with a decrease of reproductive ability were also reduced, and this also confirmed the state of oxidative stress. Thus, boars of the experimental group had a pronounced decrease in glutathione peroxidase activity by 28,4% (p ˂ 0,05), while the activity of glutathione reductase in erythrocytes was significantly reduced by 20,2% (p ˂ 0,05). The lack of physiological activity of antioxidant enzymes will have a negative impact on the fertilizing ability of sperm, because the lack of components of the enzymatic antioxidant defence system affects the activity of enzymes in sperm received from the breeding boars. A balance was observed between the components of the enzymatic antioxidant defense: the ratio of antioxidant enzymes’ activity in the male experimental group was lower than the values in the control group, however, the indices of superoxide dismutase/catalase and glutathione peroxidase/glutathione reductase had the same values, indicating that the balance in the enzymatic system of antioxidant defense was maintained. A decrease in enzyme indices in the experimental group indicated a decrease in the antioxidant potential and confirmed a certain state of oxidative stress in the breeding boars organism.

scholarly journals Protective Effect of Dorema glabrum on Induced Oxidative Stress by Diazinon in Hippocampus of Rat

2019 ◽  
pp. 1-7
Author(s):  
Mina Adampourezare ◽  
Parisa Sistani ◽  
Homeira Hatami Nemati
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Catalase Activity ◽  
Antioxidant Properties ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Male Wistar Rats ◽  
Pre Treatment

Introduction: Diazinon (DZN) administration produces lipid peroxidation as an indicator of oxidative stress in the brain. Some medicinal plants such as Dorema glabrum has antioxidant properties, so can be used as an antioxidant that may protect neurons from oxidative stress. The aim of present study was to investigate the effect of D. glabrum against DZN-induced oxidative stress in hippocampus. Methods: Twenty-four adult male Wistar rats were used in this study. The rats randomly were divided into four groups including a control group, and two groups received different doses of D. glabrum (40 and 80 mg/kg) as pre-treatment for 21 days with DZN (100 mg/Kg) that was injected intraperitoneally (ip) in last day of D. glabrum usage, and one group received only DZN. Thiobarbituric acid reactive substances (TBARS), which are the indicators of lipid peroxidation, and the activities of antioxidant enzymes (glutathione peroxidase, superoxide dismutase and catalase) were determined in the ratsʼ hippocampus. Results: Administration of DZN significantly increased TBARS levels and superoxide dismutase activity and decreased glutathione peroxidase activity but there were no significant changes in catalase activity in the hippocampus. Combined D. glabrum and DZN treatment, caused a significant increase in glutathione peroxidase, a significant decrease of TBARS and a significant decrease in superoxide dismutase and again no significant changes in catalase activity in the rats’ hippocampus when compared to the rats treated with DZN. Conclusion: Our study demonstrated that D. glabrum had an amelioratory effect on oxidative stress induced by DZN.

scholarly journals Cold-induced Antioxidant Enzymes Changes in Leucanthemum maximum`Silver Princess'

HortScience ◽  
2005 ◽  
Vol 40 (3) ◽  
pp. 546-548
Author(s):  
Suping Zhou ◽  
Roger J. Sauvé ◽  
Margaret T. Mmbaga ◽  
Chaim Frenkel
Keyword(s):  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Cold Tolerance ◽  
Catalase Activity ◽  
Guaiacol Peroxidase ◽  
Species Change ◽  
Cold Tolerant ◽  
Peroxidase Isoforms

Leucanthemum maximum `Silver Princess' plants, that were gradually acclimated for 7 days at 10 °C followed by 28 days at 7 °C, were subjected to the following cold treatments: 30 days at 4 °C; 4 or 5 days at 0 °C and for 3 hours at –1 °C to identify cold inducible proteins that may be responsible for cold tolerance in this cold tolerant species. Change in antioxidant enzymes activity in fully expanded leaves was assessed after each treatment. Catalase activity began to increase after 30 days at 4 °C and reached its peak after a 5-day exposure to 0 °C. The activity of cellular glutathione peroxidase and glutathione reductase significantly increased after a 4-day exposure to 0 °C. Changes in activity of four active superoxide dismutase isoforms, one basic guaiacol peroxidase and two o-dianisine peroxidase isoforms were also detected following the full series of cold treatments (30 days at 4 °C; 4 or 5 days at 0 °C and for 3 hours at –1 °C).

Sign in / Sign up

Export Citation Format

Share Document