scholarly journals Assessment of phenotypic and storage protein diversity in exotic barley cultivated in District Dir (Pakistan)

2019 ◽  
Vol 10 (4) ◽  
pp. 400-405
Author(s):  
M. Ali ◽  
M. Nisar ◽  
W. Khan ◽  
T. Naz ◽  
S. U. Zaman ◽  
...  
Keyword(s):  
Quantitative Traits ◽  
Storage Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Minimum Variance ◽  
Mean Value ◽  
Variability Index ◽  
Total Seed ◽  
High Level

A total of 198 exotic barley genotypes were collected from the Gene Bank of the Plant Genetic Resource Institute (PGRI), National Agriculture Research Center (NARC), Islamabad, Pakistan, for the assessment of genetic diversity based on morphological and seed storage proteins. Qualitative and quantitative traits were noted as per IPGRI, 1994 descriptor. Among the quantitative parameters, a high level of genetic variability index was noted in seeds per spike at 79.9% of coefficient of variance followed by biomass per plant which shows 37.4% variance, while minimum variance in quantitative traits was noted in days to germination at 5.4% followed by days to maturity at 3.1% with average mean genetic variation in all quantitative traits at 97.6%. Assay of total seed protein in these exotic accessions was analogue through polyacrylamide gel electrophoresis. A high level of variation was noted in loci (bands) B26 (0.98%) followed by B25 (0.89%), B24 (0.78%),B23 (0.69%) and B01 (0.52%). A similarly low level of variation was detected in B03 (0.16%) followed by B06 (0.18%), B13 (0.19%), B12 (0.21%), B11 (0.23%), B05 (0.24%), B07 (0.25%), B21 (0.34%), B20 (0.35%), B17 (0.39%). The results indicate that the mean value of variation in these accessions is 97.6%. Further assessments and exploration were suggested for these genotypes in multi-climatic zones to satisfy farmers’ need, breeders’ interest and malt-industrial requirements.

Revealing genetic diversity in land races and wild accessions of mungbean [Vigna radiata (L.) Wilczek] using SDS-PAGE of seed storage proteins

2015 ◽  
Author(s):  
Ananya Panda ◽  
Swapan K. Tripathy
Keyword(s):  
Storage Proteins ◽  
Seed Storage Protein ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Land Races ◽  
Sds Page ◽  
Wild Accessions ◽  
Total Seed

Total seed storage protein profiles of 74 mungbean land races, three wild accessions and a popular variety ‘Jyoti’ of Odisha were analysed by Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). 32 genotypes could be clearly identified based on genotype-specific seed protein fingerprints while rest of the test genotypes were categorized into eight protein types. Genotypes included in each protein type had 100% homology and some of these could be duplicates. In this pursuit, a few specific polypeptide markers have been detected for identification of the land races/ genotypes. Dendrogram based on electrophoretic data clustered the genotypes into seven groups at 70% phenon level. Paralakhemundi local, Samarjhola local and Phulbani local-D; and three wild accessions (TCR 20, TCR 213 and TCR 243) were comparatively divergent from other genotypes. Besides, Jyoti, Kalahandi local 2A, Sikri local, kodala local A and TCR 20 were identified to be protein rich with high seed yield. TCR 20 being morphologically similar to mungbean, moderately high protein content and high yielding as well as resistant to drought and bruchids; it may serve as a valuable source genotype in recombination breeding

Genetic Effects on the Proportions of Conglutin γ and a Subunit of Conglutin β in the Storage Proteins of Lupinus angustifolius L. Seeds

1981 ◽  
Vol 8 (3) ◽  
pp. 277 ◽  
Author(s):  
RN Oram ◽  
JM Gillespie ◽  
RJ Blagrove
Keyword(s):  
Storage Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Lupinus Angustifolius ◽  
Β Subunit ◽  
Partial Dominance ◽  
High Level ◽  
Conglutin Γ

The genetic variability in the proportions of conglutin β and γ components of lupin seed storage proteins was explored in F1, F2 and F3 families generated from a cross between two genotypes of Lupinus angustifolius cv. Uniharvest and a white-seeded selection from CPI 47644. The levels of conglutin γ differed by a factor of two in these parents, but varied continuously in the F2 and F3 families, indicating that the parental difference is due to several genes. These parents also differed in the level of a major conglutin β subunit, with a molecular weight of 30 000. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the storage globulins from individual F1, F2 and F3 seeds showed that the high level in cv. Uniharvest is controlled by an incompletely dominant allele, Bsc, at one locus, whereas the low level in CPI 47644 (white-seeded) is due to homozygosity for the recessive allele, bsc. The partial dominance of the Uniharvest gene suggests that the locus has a regulatory or processing function, so it has been designated beta subunit controller. F3 families differed significantly in the level of the conglutin β subunit in Bsc/- seeds, indicating that other loci with smaller effects also influence the proportion of this subunit. The proportion of the β subunit in any seed depends largely on its own genotype, and not on the genotype of the maternal plant. The proportion of conglutin γ in the storage proteins varied inversely with the proportion of the β subunit. The implications of these results for the genetic improvement of the biological value of lupin protein are discussed.

scholarly journals Phytochemical Evaluation of Moth Bean (Vigna aconitifoliaL.) Seeds and Their Divergence

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Neha Gupta ◽  
Nidhi Shrivastava ◽  
Pramod Kumar Singh ◽  
Sameer S. Bhagyawant
Keyword(s):  
Storage Proteins ◽  
Seed Storage Protein ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Molecular Weight Range ◽  
Two Dimensional Gel Electrophoresis ◽  
Moth Bean ◽  
Total Seed

In the present study, phytochemical contents of 25 moth bean (Vigna aconitifolia) seed accessions were evaluated. This includes protease inhibitors, phytic acid, radical scavenging activity, and tannins. The studies revealed significant variation in the contents of theses phytochemicals. Presence of photochemical composition was correlated with seed storage proteins like albumin and globulin. Qualitative identification of total seed storage protein abundance across two related moth bean accessions using two-dimensional gel electrophoresis (2D-GE) was performed. Over 20 individual protein fractions were distributed over the gel as a series of spots in two moth bean accessions. Seed proteome accumulated spots of high intensity over a broad range of pI values of 3–10 in a molecular weight range of 11–170 kDa. In both seed accessions maximum protein spots are seen in the pI range of 6–8.

Diversity of seed storage proteins in common wheat (Triticum aestivum L.)

2011 ◽  
Vol 9 (2) ◽  
pp. 256-259
Author(s):  
Zuzana Šramková ◽  
Edita Gregová ◽  
Svetlana Šliková ◽  
Ernest Šturdík
Keyword(s):  
Triticum Aestivum ◽  
Common Wheat ◽  
Gel Electrophoresis ◽  
Storage Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Triticum Aestivum L ◽  
Polyacrylamide Gel

The objective of our study was to determine the composition of high-molecular weight-glutenin subunits (HMW-GS) in 120 cultivars of common wheat (Triticum aestivum L.). Fourteen alleles and 34 allelic compositions were detected using sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The most frequent HMW-GS alleles at the Glu-A1, Glu-B1 and Glu-D1 loci were null (57.1%), 7+9 (43.3%) and 5+10 (61.9%), respectively. However, low-frequency HMW-GS alleles were also observed, such as 13+16, 20, 21, 7 and 18, encoded by the Glu-B1 locus, and 4+12, encoded by the Glu-D1 locus. The wheat–rye 1BL.1RS translocation was identified in 25 cultivars, using acid polyacrylamide gel electrophoresis. The Glu-score varied greatly, and some lines reached the maximum value of 10.

scholarly journals Genetic diversity of the Algerian peanut population analyzed using morphological markers and seed storage proteins

2021 ◽  
Vol 182 (3) ◽  
pp. 111-124
Author(s):  
H. Djeghim ◽  
I. Bellil ◽  
D. Khelifi
Keyword(s):  
Storage Proteins ◽  
Phenotypic Diversity ◽  
Protein Profile ◽  
Principal Component ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
African Countries ◽  
Seed Shape ◽  
Major Interest ◽  
High Level

Background. The peanut is one of the most important oil crops suitable for cultivation in the tropical areas of the world. Despite its agronomic importance, few studies have been carried out to assess the morphogenetic diversity of Arachis hypogaea L., especially in East African countries. The major interest of this morphologic study lies in the potential of this species to provide useful genes for the improvement of cultivated peanuts. To date, no study has been performed in Algeria to characterize local peanut varieties.Materials and methods. Thirty peanut accessions were collected from four principal areas of peanut production in Algeria. Genetic characterization using 15 agronomic characters and 25 morphological descriptors showed a high level of diversity among accessions. Principal Component Analysis and the Hierarchical Ascendant Classification were made to clarify the genetic relationship between peanut accessions.Results and discussion. Results showed that leaflet size (length and width), seed shape and size, oil content, and branching pattern were the principal characters to discriminate the screened A. hypogaea accessions. In addition to that, the weights of 10 pods and 100 seeds were the most variable traits and presented a CV of 42.53% and 40.12%, respectively. On the other hand, total storage proteins extracted were separated using SDS-PAGE and revealed thirty bands that were used to generate a matrix and make a cluster analysis using the UPGMA method, exhibiting different storage proteins compositions. Moreover , the phenotypic diversity observed agrees with the storage protein profile diversity, while the accessions grouped in similar clusters belong to the two subspecies of A. hypogaea. The results of the current study show that morphological traits and seed storage proteins can be useful for exploring the diversity among A. hypogaea accessions.

SDS–PAGE of seed proteins in Festuca (Poaceae): taxonomic implications

1991 ◽  
Vol 69 (7) ◽  
pp. 1425-1432 ◽  
Author(s):  
S. G. Aiken ◽  
S. E. Gardiner
Keyword(s):  
Native Species ◽  
Storage Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Seed Proteins ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Banding Patterns ◽  
Ungerminated Seed ◽  
Sds Page ◽  
Species Specific

Taxonomically useful descriptors were provided by the banding patterns of seed storage proteins obtained when extracts of bulked, ungerminated seed samples from commercially available North American native species of Festuca were analyzed by sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS–PAGE). The banding patterns for three species of rough fescues (section Breviaristatae Krivot) indicate that although the taxa are closely related, F. campestris Rydb. (2n = 56) does not appear to be an autoploid of either F. altaica Trin. (2n = 28) or F. hallii (Vasey) Piper (2n = 28). A distinct band corresponding to a molecular weight of 57 000 occurred in the seed protein profiles of all native and commercial samples of Festuca L. analyzed. The profile for F. californica Vasey, questionably section Breviaristatae, also has a band at this position, and is very different from that of F. altaica, F. campestris, and F. hallii. Species-specific banding patterns were observed for F. brachyphylla Schultes, F. saximontana Rydb., F. idahoensis Elmer, and F. trachyphylla (Hackel) Krajina (F. ovina L. s.l., F. longifolia Thuill., F. ovina var. duriuscula auct. amer.). The results support the recognition of subgenus Schedonorus (Beauv.) Peter., and sections Breviaristatae Krivot and Festuca. Key words: Poaceae, Festuca, SDS–PAGE seed proteins.

Placement of loci for avenins and resistance to Puccinia coronata to a common linkage group in Avena strigosa

Genome ◽  
1994 ◽  
Vol 37 (6) ◽  
pp. 900-903 ◽  
Author(s):  
P. J. Rayapati ◽  
V. A. Portyanko ◽  
M. Lee
Keyword(s):  
Linkage Group ◽  
Storage Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Seed Proteins ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Puccinia Coronata ◽  
A Genome ◽  
Genes Encoding ◽  
Group 1 Chromosomes

Alcohol-soluble seed storage proteins of oat (avenins) were extracted from two diploid accessions representing the A genome and separated by high-resolution acid polyacrylamide gel electrophoresis. Polymorphisms were detected for three clearly resolved protein bands. Linkage analysis of 88 F2:3 families mapped the three bands to a single locus. Integration of avenin segregation data with an RFLP linkage map constructed from the same population, mapped the avenin locus to a linkage group containing a locus conferring resistance to nine isolates of Puccinia coronata. Linkage between genes encoding alcohol-soluble seed proteins and genes for resistance to Puccinia species was also observed for the homoeologous group 1 chromosomes of barley (1H), rye (1R), wheat (1A, 1B, 1D), and chromosomes 4 and 10 of maize.Key words: genetics, seed protein, disease resistance.

scholarly journals Characterization of Tunisian castor bean genotypes using SDS-PAGE of total seed storage proteins

10.5219/973 ◽  
2018 ◽  
Vol 12 (1) ◽  
Author(s):  
Martin Vivodík ◽  
Ezzeddine Saadaoui ◽  
Zdenka Gálová ◽  
Želmíra Balážová ◽  
Lenka Petrovičová
Keyword(s):  
Castor Bean ◽  
Storage Proteins ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Sds Page ◽  
Total Seed
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