scholarly journals The influence of the synthetic food colourings tartrazine, allura red and indigo carmine on the body weight of Tenebrio molitor (Coleoptera, Tenebrionidae) larvae

2018 ◽  
Vol 9 (4) ◽  
pp. 479-484
Author(s):  
V. O. Martynov ◽  
V. V. Brygadyrenko

Substances for protecting plants often contain colourings, the impact of which on invertebrates has been studied insufficiently. The addition of food colourings in different concentrations to the diet of saprophage beetles can affect their metabolism, causing loss of body weight. In the experiment, we determined the impact of tartrazine, allura red and indigo carmine on the body weight of Tenebrio molitor Linnaeus, 1758 larvae. The substances were added to their fodder at five concentrations (1, 0.1, 0.01, 0.001 and 0.0001 g/kg of dry fodder) during a 21-day experiment. Statistically significant data on changes in the body weight of T. molitor larvae were received after adding 1 g/kg concentration of indigo carmine and 0.1 and 1 g/kg concentrations of tartrazine. In the other variants of the experiment, no statistically significant differences were determined. Tartrazine, allura red and indigo carmine cause decrease in the body weight of T. molitor larvae, depending on the concentration of the colouring. The toxic effect of synthetic food colourings on living organisms and the low number of studies devoted to such impact on insects indicate the relevance and necessity for further research in this sphere.

2019 ◽  
Vol 4 (1) ◽  
pp. 1-4
Author(s):  
MA Ebrahiem

The study aimed to investigate the impact of supplementary feeding on the young camels calve growth rate and daily gain. A total of 16 heads of young male camel calves from the Arabic camel breeds were selected and div ided into four equal groups (1, 2,3,4). Four heads of camel calves in each group, their body weight ranged from 176 - 220 kg. Experimental animal in group one was control supplemented nothings jus t depend on the natural range land, group two was feed on watermelon seeds, group three was allowanced concentrated diet composed of ground nut cake and the animals in group four intake mixture diet consist of watermelon seeds and ground nut cake 50% for e ach. The body weight of the experimental animal was determined biweekly using table balance. The random complete experimental design was used and the Analysis of variance and Tukey test were applied for data analysis and means separation. The results indic ated that the average Body weights of the camel calves were 174.75±3.3, 275.93± 9.0, 241.50± 8.5 and 236.50±8.2 Kg, in group one, two, three and four respectively. The higher body weight was recorded in group two (275.93±9 Kg) in which the animals feed on watermelon seeds and followed by group three and four (241.50± 8.5 and 236.50±8.2) respectively. On the other hand the less camel calves body weight was obtained in group one (control) that experimental animals not intake supplementary feeding. Highly diff erences (P ≤ 0.01) were obtained in the body weight between the groups. The daily gain of the first group, second, third and fourth were 214±12g, 624±28g, 542±19g and 528±12g, respectively, while no significant differences (P ≥ 0.01) between diets groundnut cake 242 kg and mixture diet 237 kg. The protein content was higher in ground nut cake 41% when compared to the protein content in watermelon seeds 36%, on the other hand the percentage of fat was highest in watermelon seeds 27.8% compared to ground nut ca ke 13.4%.


2016 ◽  
Vol 72 (12) ◽  
pp. 755-759 ◽  
Author(s):  
Monika Bryl ◽  
Hanna Bis-Wencel ◽  
Brygida Ślaska ◽  
Bożena Nowakowicz-Dębek ◽  
Zbigniew Bełkot ◽  
...  

The aim of this study was to assess the impact of the leptin concentration and body weight of mink females on the birth parameters of their kits. Blood samples were collected 3 times during preparation for reproduction, 7±2 days before mating. Total leptin concentration in serum was measured using commercial RIA kits (Millipore, St. Charles, Missouri USA). Research was conducted on 20 clinically healthy scanbrown female minks (Neovison Vison) aged 2-3 years. To evaluate their body condition, the females were divided into two groups based on the BCS scoring system. In the first group, referred to as BCS 2, lean females were included, and the second group (BCS 4) consisted of obese females. In mink dams belonging to BCS 2, the average leptin values were one-fourth of those in the BCS 4 group. The results obtained indicate the influence of the body mass of minks during preparation for reproduction on serum leptin concentration. The results of the Mann-Whitney U test showed a statistically significant difference between weights and leptin concentrations in groups BCS 2 and BCS 4. Among the other traits, such differences were not found. The analysis of results obtained in the two mink groups did not reveal a clear relationship between leptin in the serum of mothers before mating and the birth parameters of their kits.


2018 ◽  
Vol 45 (1) ◽  
pp. 24-32 ◽  
Author(s):  
Vladyslav O. Martynov ◽  
Viktor V. Brygadyrenko

Abstract Addition of low concentrations of metal ions to the diet of saprophagous insects can impact on their metabolism over a short period of time, causing an increase or decrease in their body mass. This article presents a 14-day laboratory experiment evaluating the changes in the body mass of larval stage 3 of Tenebrio molitor (Linnaeus, 1758) induced by adding different inorganic substances (350 mg kg−1 of dry fodder) to the diet of the larvae. Following the addition of inorganic substances to the fodder, the most marked differences compared to the control were observed in the groups which consumed substrate with lead nitrate (the mass of the larvae increased on average by 102.6% compared to increase in mass in the control variant of the experiments), cobalt nitrate (by 96.9%), calcium chloride (by 89.1%) sodium triphosphate (by 86.0%), zinc chloride (by 83.5%). A nonsignificant effect (a tendency of increase in the body mass) on T. molitor larvae was caused by manganese sulfate (by 57.8%), aluminium nitrate (by 57.3%), iron oxide (by 51.5%), barium nitrate (by 47.9%), orthophosphoric acid (by 47.4%), manganese chloride (by 46.5%), calcium carbonate (by 27.7%), iron sulfate (by 24.2%) and ammonium heptamolybdate (by –7.5%). Therefore, 5 out of the 15 studied inorganic substances significantly stimulated the increase in the body weight of T. molitor larvae, and 7 manifested these capacities at the level of tendency (stimulated an increase in body weight averaging 43–58% over the 14-day experiment). The obtained data indicate a necessity for further study on the impact of inorganic pollutants on different stages of insects.


2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 1038-1038
Author(s):  
Michael Miklus ◽  
Pedro Prieto ◽  
Cynthia Barber ◽  
Robert Rhoads ◽  
Samer El-Kadi

Abstract Objectives The objectives of this study were to determine the effect of 2’fucosyllactose (2’FL) and fat blends on growth, body composition and fatty acid profile of the liver and brain using the neonatal pig as a model for the human infant. Methods Pigs (3 d old) were randomly assigned to either: 1. control, 2. Palm Olein (PO) fat blend – Low 2'-FL, 3. PO – High 2'-FL, 4. High oleic acid (HO) – Low 2'-FL, 5. HO FB – High 2'-FL, 6. PO FB – GLA, or 7. kept with their sows. Pigs in groups 1 to 6 received 250 ml·kg−1·d−1 of formula in 5 equal meals for 15 d. On day 14 of the study, groups 1–6 received intraperitoneal E. coli LPS challenge at 100 µg·kg−1 weight. Results Body weight was greater for piglets fed by sows than those in the other groups (P < 0.001). In addition, % fat and bone mineral content were higher in the sow-fed group while lean % was less sow-fed piglets (group 7) compared with those in the other groups (P < 0.05). Only longissimus weight expressed as a % of body weight, was greater for group 7 compared with all other groups (P < 0.001). Soleus, semitendinosus, brain, heart and spleen weights as a % of body weight were similar across all groups. However, liver weight as a % of body weight was greater in groups 1–6 (3.7%) compared with group 7 (2.8%; P < 0.001). The proportion of brain 16:1 fatty acid was less (0.83%) for groups 1–6 than for group 7 pigs (1.08%; P < 0.0001). The proportion of 20:3 N6 was greatest (0.66%) for group 3 compared with groups 1 and 4 (0.55%; P < 0.05). In addition, the proportion of 20:5 N3 was greatest (0.12%) for group 3 compared with groups 1 and 7 (0.07%; P < 0.05). The proportion of liver 16:1, 18:0, and 18:1 cis-11 fatty acids were greater for group 7 (2.3, 23, 2.2%) than groups 1–6 (0.2, 20, 1.2%; P < 0.0001). Conversely, the contribution of 14:0, 18:1 cis-9, 18:3 N6 cis-6,9,12, and 22:6 N3 were greater for pigs in groups 1–6 (1.3, 0.6, and 14, 7.8%) compared with those in group 7 (0.5, 8.5, 0.2 and 3.5%; P < 0.0001). Conclusions Our data suggest that feeding 2’fucosyllactose had no effect on the body weight gain and composition in neonatal pigs. Our data also suggest that dietary fatty acids have a greater effect on liver than on brain fatty acid composition. Funding Sources Funding for the work was provided by Perrigo Nutritionals, LLC.


Author(s):  
Pablo A. Scacchi Bernasconi ◽  
Nancy P. Cardoso ◽  
Roxana Reynoso ◽  
Pablo Scacchi ◽  
Daniel P. Cardinali

AbstractCombinations of fructose- and fat-rich diets in experimental animals can model the human metabolic syndrome (MS). In rats, the increase in blood pressure (BP) after diet manipulation is sex related and highly dependent on testosterone secretion. However, the extent of the impact of diet on rodent hypophysial-testicular axis remains undefined. In the present study, rats drinking a 10% fructose solution or fed a high-fat (35%) diet for 10 weeks had higher plasma levels of luteinizing hormone (LH) and lower plasma levels of testosterone, without significant changes in circulating follicle-stimulating hormone or the weight of most reproductive organs. Diet manipulation brought about a significant increase in body weight, systolic BP, area under the curve (AUC) of glycemia after an intraperitoneal glucose tolerance test (IPGTT), and plasma low-density lipoprotein cholesterol, cholesterol, triglycerides, and uric acid levels. The concomitant administration of melatonin (25 μg/mL of drinking water) normalized the abnormally high LH levels but did not affect the inhibited testosterone secretion found in fructose- or high-fat-fed rats. Rather, melatonin per se inhibited testosterone secretion. Melatonin significantly blunted the body weight and systolic BP increase, the increase in the AUC of glycemia after an IPGTT, and the changes in circulating lipid profile and uric acid found in both MS models. The results are compatible with a primary inhibition of testicular function in diet-induced MS in rats and with the partial effectiveness of melatonin to counteract the metabolic but not the testicular sequelae of rodent MS.


2020 ◽  
Vol 11 (5) ◽  
pp. 489-509
Author(s):  
R. Cheng ◽  
H. Liang ◽  
Y. Zhang ◽  
J. Guo ◽  
Z. Miao ◽  
...  

This study aimed to determine the impact of Lactobacillus plantarum PC170 concurrent with antibiotic treatment and/or during the recovery phase after antibiotic treatment on the body weight, faecal bacterial composition, short-chain fatty acids (SCFAs) concentration, and splenic cytokine mRNA expression of mice. Orally administrated ceftriaxone quantitatively and significantly decreased body weight, faecal total bacteria, Akkermansia muciniphila, and Lactobacillus plantarum, and faecal SCFAs concentration. Ceftriaxone treatment also dramatically altered the faecal microbiota with an increased Chao1 index, decreased species diversities and Bacteroidetes, and more Firmicutes and Proteobacteria. After ceftriaxone intervention, these changes all gradually started to recover. However, faecal microbiota diversities were still totally different from control by significantly increased α- and β-diversities. Bacteroidetes all flourished and became dominant during the recovery process. However, mice treated with PC170 both in parallel with and after ceftriaxone treatment encouraged more Bacteroidetes, Verrucomicrobia, and Actinobacteria, and the diversity by which to make faecal microbiota was very much closer to control. Furthermore, the expression of splenic pro-inflammatory cytokine tumour necrosis factor-α mRNA in mice supplemented with PC170 during the recovery phase was significantly lower than natural recovery. These results indicated that antibiotics, such as ceftriaxone, even with short-term intervention, could dramatically damage the structure of gut microbiota and their abilities to produce SCFAs with loss of body weight. Although such damages could be partly recovered with the cessation of antibiotics, the implication of antibiotics to gut microbiota might remain even after antibiotic treatment. The selected strain PC170 might be a potential probiotic because of its contributions in helping the host animal to remodel or stabilise its gut microbiome and enhancing the anti-inflammatory response as protection from the side effects of antibiotic therapy when it was administered in parallel with and after antibiotic treatment.


Animals ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 2679
Author(s):  
Lihuai Yu ◽  
Hongmin Li ◽  
Zhong Peng ◽  
Yuzhu Ge ◽  
Jun Liu ◽  
...  

This study examined the impact of early weaning on antioxidant function in piglets. A total of 40 Duroc × Landrace × Large White, 21-day-old piglets (half male and half female) were divided into suckling groups (SG) and weaning groups (WG). Piglets in WG were weaned at the 21st day, while the piglets in SG continued to get breastfed. Eight piglets from each group were randomly selected and slaughtered at 24th-day (SG3, WG3) and 28th-day old (SG7, WG7). The body weight, liver index, hepatocyte morphology, antioxidant enzymes activity, gene expression of antioxidant enzymes, and Nrf2 signaling in the liver of piglets were measured. The results showed that weaning caused decreased body weight (p < 0.01), lower liver weight (p < 0.01), and decreased the liver organ index (p < 0.05) of piglets. The area and size of hepatocytes in the WG group was smaller than that in the SG group (p < 0.05). We also observed that weaning reduced the activity of superoxide dismutase (SOD) and catalase (CAT) (p < 0.05) in the liver of piglets. Relative to the SG3 group, the gene expression of GSH-Px in liver of WG3 was significantly reduced (p < 0.05). The gene expression of Nrf2 in the SG3 group was higher than that in the WG3 group (p < 0.01). The gene expression of NQO1 in the SG7 group was higher than that in the WG7 group (p < 0.05). In conclusion, weaning resulted in lower weight, slowed liver development, and reduced antioxidant enzymes activity, thereby impairing liver antioxidant function and suppressing piglet growth.


2014 ◽  
Vol 3 (1) ◽  
pp. 57
Author(s):  
Abdelaziz Ghanemi ◽  
Besma Boubertakh

Pollution  represents  a  problem  common  to economy and  public  health. Indeed, the public health, because of the  divers’  type of pollutions, is facing divers challenges for which urgent solutions are required.The biology provides approaches not only to deal with the pollution, but also to  obtain  economic  benefits. Some living  organisms  have  particular metabolisms  that allow  them  to  assimilate  and  metabolite  the polluting agents  and thus reduce the  impact  they have on both environment  and public health.  On  the other  hand,  the  metabolic  properties  of  specific organisms make  the  polluting  elements raw materials to  synthesize  other elements that are benefits  for  economy  and  non-toxic  for  the  ecology and  the  biohealth. Yet, other options such as the regulations and laws are  required  to improve the efficiency of these approaches.


2007 ◽  
Vol 23 (1-2) ◽  
pp. 13-20
Author(s):  
Andrey Kurtenkov

It is related leg problems to the realization of the necessity of doing a detailed analysis of the phenotype correlations between body weight and exterior measurements. As a result of the study, lower coefficients have been obtained of the correlation between the girth of the tarso metatarsus on one hand, and the body weight and the girth behind the wings, on the other hand (respectively 0.563 and 0.608), compared with the one between the body weight and the girth behind the wings (0.898). It is advisable in the selection of ostriches to take into consideration the necessity of a higher phenotypic correlation between the girth of the tarso metatarsus on the one hand, and the body weight and the girth behind the wings on the other hand, with a view to preventing leg problems.


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