scholarly journals Cytogenetic anomalies of winter wheat cells, induced by chemical contamination of the territory of Kalush industrial district

2018 ◽  
Vol 9 (3) ◽  
pp. 446-452 ◽  
Author(s):  
V. V. Morgun ◽  
R. A. Yakymchuk
Keyword(s):  
Winter Wheat ◽  
Soil Contamination ◽  
Raw Materials ◽  
Mutagenic Activity ◽  
Water Solution ◽  
Control Level ◽  
Soil Samples ◽  
Toxic Waste ◽  
Chemical Contamination ◽  
High Concentrations

Mass accumulation of toxic waste near inhabited localities has changed some regions of Ukraine, in particular Kalush industrial area (Ivano-Frankivsk region), into zones of ecological disaster. Research on cytogenetic anomalies caused by chemical soil contamination of the territories of toxic chemical warehouses will be useful in determining the level of mutagenic activity of xenobiotics when they enter the environment and potential mechanisms of the induction of chromosome reconstructions by them and mitosis disorders. The aim of the research is to study frequency and spectrum of the types of cytogenetic disorders in T. aestivum L. under the prolonged effect on the seeds of soil contaminated with hexachlorinebenzene from territories of toxic waste warehousing and to determine the level of their mutagenic activity as compared with the effect of moderate and high concentrations of N-nitrozo-N-methylurea (NMU). Seeds of winter wheat cultivars Al’batros odes’kyi and Zymoiarka were sprouted in the soil samples taken from the toxic waste ground of LLC “Oriana Halev”, its recultivated area and the dump area of Dombrovskyi potash ore mine, situated near Kalush city. Hexachlorobenzene concentrations in the soil of the studied areas exceeded CPC by 1233–18350 times. Soil samples from a tentatively clean area of Svatky village, Hadiach district, Poltava region were taken as the control. To study cytogenetic consequences of the effect of moderate and high concentrations of NMU, wheat seeds were kept in a mutagen water solution at concentrations 0.005%, 0.010%, 0.025%. Frequency and spectrum of cytogenetic anomalies were determined in the cells of sprout root meristem using the anatelophase technique. Chemical contamination of the soil exhibited high mutagenic activity which, by induction frequency of cytogenetic anomalies, exceeded the control level by 1.8–3.8 times and equalled mutagenic activity of NMU in moderate concentrations. The highest level of cytogenetic disorders, which exceeded spontaneous indicators by 3.4–3.8 times, was found when the soil contamination of the territory of the toxic waste ground with hexachlorobenzene was the most intensive. Traces of hexachlorobenzene in the soil of the recultivated plot of the ground continue to manifest high cytogenetic activity and pose a threat for the genomes of living organisms. Frequency of chromosome aberrations at a low hexachlorobenzene concentration in the soil of the disposal area of Dombrovskyi mine exceeded spontaneous indicators by 1.8–2.4 times, which is the result of its complex effect with natural-mineral compounds of mining-chemical raw materials. The increase of some bridges and acentric chromosome rings – markers of a radiation effect – among the types of cytogenetic disorders, induced by the soil contamination with hexachlorobenzene, confirms the radiometric properties of the xenobiotic, which were identified at high concentrations of NMU. The increase in the number of the cells with multiple aberrations, induced by the hexachlorоbenzene contamination of the soil holding the studied objects proves the high genotoxicity of the chemical compound and the threat of serious genetic consequences if it enters the environment.

scholarly journals Assessment of mutagenic activity of toxic waste polygon (Kalush) soil’s chemical pollution by the level of cytogenetic disorders in Triticum aestivum L.

2020 ◽  
Vol 27 ◽  
pp. 293-298
Author(s):  
V. F. Valyuk ◽  
R. A. Yakymchuk ◽  
L. Yu. Sobolenko ◽  
S. I. Sorokina
Keyword(s):  
Triticum Aestivum ◽  
Chromosomal Aberrations ◽  
Raw Materials ◽  
Mutagenic Activity ◽  
Control Level ◽  
Triticum Aestivum L ◽  
Toxic Waste ◽  
Chemical Pollution ◽  
Chemical Contamination ◽  
High Concentrations

Aim. To study the frequency and spectrum of cytogenetic disorders in Triticum aestivum L. with prolonged action on soil xenobiotic seeds of toxic waste storage areas and to explore the level of their mutagenic activity in comparison with the action of NMS’s moderate and high concentrations. Methods. Cytogenetic disorders induced by soil’s chemical contamination of toxic waste landfill of Dombrovsky Quarry dump (Kalush city) and influence of NMS’s moderate and high concentrations were determined in the cells of the meristem root using the ana-thelophase method. Results. Soil’s chemical contamination of the studied objects exhibits high mutagenic activity, which exceeds the control level by 1.8–3.8 times and does not yield to the mutagenic activity of NMS’s moderate concentrations. The high frequency of chromosomal aberrations at the low concentration of hexachlorobenzene in the soil of the Dombrovsky Quarry dumps is a consequence of its complex interaction with natural mineral compounds of the mountain chemical raw materials. Conclusions. The increasing of the spectrum of cytogenetic disorders induced by chemical soil contamination the proportion of bridges and the chromosomal rings testify radiomimetic properties of the mutagenic factors. The increasing of the number of cells with multiple aberrations indicates a high genotoxicity of hexachlorobenzene and the risk of serious genetic consequences in the case of falling it’s into the environment. Keywords: Triticum aestivum L., toxic waste, mitosis disorders, chromosomal aberrations, mutagenic activity.

scholarly journals Soil mutagenic activity in hazardous waste site of Kalush City (Western Ukraine)

2018 ◽  
Vol 8 (1) ◽  
pp. 880-886 ◽  
Author(s):  
R.A. Yakymchuk ◽  
V.F. Valyuk
Keyword(s):  
Soil Contamination ◽  
Raw Materials ◽  
Mutagenic Activity ◽  
Control Level ◽  
Cell Number ◽  
Activity Level ◽  
Toxic Waste ◽  
Mitotic Apparatus ◽  
Cytogenetic Aberrations ◽  
High Concentrations

<p>Frequency and spectrum of cytogenetic disorders in the cells of root meristem sprouts of <em>Triticum aestivum L.,</em> induced by soil contamination with hexachlorobenzene in the territory of toxic waste ground and Dombrovskyi potassium ore mine (Kalush city, Ivano-Frankivsk oblast), were studied. The concentration of hexachlorobenzene exceeded MPC (maximum permissible concentration) by 1233.3–18350 times. It has been established that chemical soil contamination causes the increase of the frequency of cytogenetic aberrations by 1.8-3.8 times. Its highest indicators were found under the most intensive soil contamination with hexachlorobenzene in the areas of toxic waste ground which were not recultivated. Frequency of cytogenetic disorders exceeded the control level by 3.4-3.8 times. The maintenance of high mutagenic activity of hexachlorobenzene remains in the soil of the recultivated area of the ground is confirmed by statistically reliable increase of the number of aberrant cells by 2.6-3 times. It has been shown that soil contamination of Dombrovskyi mine with the complex of natural-mineral compounds of mining-chemical raw materials causes the increase of mutagenic activity level of low concentrations of chemical gene-toxic factors of techno-genic origin, which is seen in the induction of higher level of cytogenic disorders compared with spontaneous indicators by 1.8-2.4 times. Spectrum of chromosome aberrations, which mostly included acentric fragments, expanded due to induced bridges, ring chromosomes and micronuclei. The share increase of the cells with dicentrics, caused by the effect of soil contamination with hexachlorobenzene in the territory of toxic waste ground, confirms the radiomimetic effect, resulted from the effect of chemical mutagen in high concentrations. Chemical pollutants of the soil of all the studied areas show the ability to cause mitosis disorder, connected with the damage of mitotic apparatus. A significant increase of disorder frequency of chromosome segregation can be the result of the interaction of mutagens not only with microtube proteins of division spindle but also with heterochromatin of near-center areas of chromosomes, which is typical for the mechanism of the emergence of cytogenetic aberrations under the effect of chemical supermutagens in high concentrations. The increase of the cell number with multiple aberrations under the effect of soil contamination with hexachlorobenzene in different concentrations proves high gene-toxicity of the chemical compound and the threat of severe genetic outcomes when it gets into the environment.</p>

scholarly journals Fe3O4@C Nanoparticles Synthesized by In Situ Solid-Phase Method for Removal of Methylene Blue

Nanomaterials ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 330
Author(s):  
Hengli Xiang ◽  
Genkuan Ren ◽  
Yanjun Zhong ◽  
Dehua Xu ◽  
Zhiye Zhang ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Solid Phase ◽  
Raw Materials ◽  
Phase Method ◽  
Maximum Adsorption Capacity ◽  
High Catalytic Activity ◽  
Solid Phase Reaction ◽  
Phase Reaction ◽  
High Concentrations

Fe3O4@C nanoparticles were prepared by an in situ, solid-phase reaction, without any precursor, using FeSO4, FeS2, and PVP K30 as raw materials. The nanoparticles were utilized to decolorize high concentrations methylene blue (MB). The results indicated that the maximum adsorption capacity of the Fe3O4@C nanoparticles was 18.52 mg/g, and that the adsorption process was exothermic. Additionally, by employing H2O2 as the initiator of a Fenton-like reaction, the removal efficiency of 100 mg/L MB reached ~99% with Fe3O4@C nanoparticles, while that of MB was only ~34% using pure Fe3O4 nanoparticles. The mechanism of H2O2 activated on the Fe3O4@C nanoparticles and the possible degradation pathways of MB are discussed. The Fe3O4@C nanoparticles retained high catalytic activity after five usage cycles. This work describes a facile method for producing Fe3O4@C nanoparticles with excellent catalytic reactivity, and therefore, represents a promising approach for the industrial production of Fe3O4@C nanoparticles for the treatment of high concentrations of dyes in wastewater.

Toxic Effects of Chromium Acetate Hydroxide on Cells Cultivated In Vitro

2001 ◽  
Vol 29 (2) ◽  
pp. 163-177 ◽  
Author(s):  
Emil Rudolf ◽  
Jan Peychl ◽  
Miroslav Červinka
Keyword(s):  
Dna Analysis ◽  
Cell Damage ◽  
Toxic Waste ◽  
Toxic Effects ◽  
Waste Materials ◽  
In Vitro Tests ◽  
High Concentrations ◽  
Discrete Manner ◽  
Chromium Acetate

Many human activities, particularly industrial ones, result in an ever-growing production of toxic waste materials. The dynamics of the toxic effects of chromium acetate hydroxide, which is found in high concentrations in a waste sediment produced in the Czech Republic, were assessed by using a battery of in vitro tests carried out on two cell lines: L-929 (mouse fibroblasts) and Hep 2 (human laryngeal cells). Various markers of cell damage were assessed by phase-contrast, video and fluorescence microscopy, fluorometry, and DNA analysis. Chromium acetate hydroxide, over a concentration range of 1–0.02mol/l induced immediate cell death by fixation, whereas, at 0.002mol/l, the treated cells died in a much slower, more discrete manner. All the detected markers of cell damage, whether immediate or slow, clearly demonstrated that the cells died by necrosis. On the other hand, test concentration of 0.001mol/l appeared to constitute a threshold at which no pathological changes of Hep 2 cells were observed over 96 hours. We conclude that chromium acetate hydroxide has a high toxic potential in vitro, which should be considered when studying the toxicity of waste materials containing it.

scholarly journals HYGIENIC ASSESSMENT OF THE CONTENT OF CHEMICALS IN THE SOIL OF MINING AREAS OF THE TRANS-BAIKAL REGION

2019 ◽  
Vol 98 (4) ◽  
pp. 400-410 ◽  
Author(s):  
L. A. Mikhailova ◽  
M. A. Solodukhina ◽  
O. G. Alekseeva ◽  
N. M. Burlaka ◽  
S. E. Lapa
Keyword(s):  
Heavy Metals ◽  
Soil Contamination ◽  
Chemical Elements ◽  
Raw Materials ◽  
Atomic Absorption Spectrophotometry ◽  
Arsenic Content ◽  
Toxic Substances ◽  
Residential Areas ◽  
Entire Area ◽  
Mining Areas

Introduction. Intensive exploration and processing of mineral raw materials in the Trans-Baikal territory has caused the accumulation of considerable amount of industrial mining waste with high content of chemical elements of different classes of hazard. Currently 33 tailings storage facilities (TSF) accumulating approximately 3 milliard tons of different industrial waste are located in the territory of the region. The aim of the research is the hygienic assessment of soil contamination in the residential areas adjacent to TSFs. Material and methods. Atomic absorption spectrophotometry method was used for the determination of heavy metals. The study presents the results of analyses of 444 samples of gross content of lead, zinc, copper, mercury, arsenic, and cadmium in the soil of Khapcheranga, Sherlovaya Gora, Kadaya, Vershino-Darasunsky, and Vershino-Shakhtaminsky mining villages throughout the duration of 2012 - 2015. Results. During the period of the study the total value of soil contamination with Zc calculated by the median concentrations in Khapcheranga Village amounted to 4.7 6.9, in Vershino-Shakhtaminaky - to 6.7 8.8, which corresponds to the «allowable» level of the contamination. Zc calculation by maximum concentrations has shown the soil to be referred to the category from “moderately dangerous” to “extremely dangerous”; this value in Khapcheranga Village amounted to 48.7 - 235.3, in Vershino-Shakhtaminsky Village - to 23.76 - 164.8. Discussion. In the residential areas the allowable degree of soil contamination was determined to be predominantly observed; Khapcheranga and Vershino-Shakhtaminsky villages are the exceptions. The results of the assessment give the evidence of tge increased lead, cadmium and arsenic content throughout the entire area of the villages, while the highest levels of accumulated toxic substances are registered in the areas located near the TSF Conclusion. Thus, several zones of natural and anthropogenous contamination with increased concentrations of heavy metals and arsenic have been formed in the Trans-Baikal Territory. This dictates the need to study the influence of geochemical anomalies on the health of population.

scholarly journals State of Heavy Metals Pollution of Flooded Agricultural Land in the North Part of Republic of Srpska

2017 ◽  
Vol 17 (1) ◽  
pp. 19
Author(s):  
Tihomir Predić ◽  
Petra Nikić Nauth ◽  
Bojana Radanović ◽  
Alen Predić
Keyword(s):  
Heavy Metals ◽  
Soil Contamination ◽  
Agricultural Land ◽  
Total Content ◽  
Soil Samples ◽  
Available Phosphorus ◽  
The North ◽  
North Part ◽  
Grid Points ◽  
Phosphorus And Potassium

This paper presents the results of the research of soil contamination in flooded area located at the confluence of Bosna and Sava rivers. The sampling was performed according to a network of points Etrs89 reference European Network based on grid points of 500 m x 500 m. The total number of soil samples was 31 and 13 samples of sediment-mud. In the soil samples and sediment-mud samples various parameters were analyzed: pH H2O and 1 M KCl, humus (method by Tjurin), and the readily available phosphorus and potassium (AL method). The interpretation of total content of heavy metals in the soil was conducted according to Dutch Soil Remediation Circular (2009). The samples were analyzed for the total content of heavy metals: Pb, Cd, Cr, Ni, Zn, Cu (aqua regia digestion, AAS) and the elevated concentrations of Ni. However, Ni content in mud was from 240.5 mg/kg to 294.5 mg/kg, in soil it was from 128.9 mg/kg to 452.1 mg/kg. All mud samples were alkaline, and 79% of soil samples were alkaline and of neutral reaction. The analysis indicated that the flood in May 2014 was not caused by soil contamination with heavy metals, which however contributed to the increase of the overall content of these elements in the soil.

scholarly journals Determination Contents of Total Phenolic Pigments and Spectrophotometric Characterization of Crude Extracts of Ten Tinctorial Plants of Niger which is Usable in Solar Energy

2018 ◽  
Vol 14 (33) ◽  
pp. 389
Author(s):  
Souley Kallo Moutari ◽  
Ayouba Mahamane Abdoulkadri ◽  
Abdourahamane Saidou Boulhassane ◽  
Adamou Rabani ◽  
Ikhiri Khalid
Keyword(s):  
Solar Energy ◽  
Raw Materials ◽  
Dye Sensitized Solar Cells ◽  
Electrical Energy ◽  
Total Phenolic ◽  
Environmental Friendliness ◽  
Pigment Synthesis ◽  
Chlorophyll Pigments ◽  
Crude Extracts ◽  
High Concentrations

Dye Sensitized-Solar Cells (DSSCs) have been extensively investigated because of their environmental friendliness and low production cost. At the heart of these is the photosensitive pigment that plays a key role in the collection of sunlight radiations and its conversion into electrical energy. However, the toxicity and the cost of pigment synthesis limit the increased use of these prototypes. Natural pigments can be an effective, sustainable, and inexpensive alternative. This paper focuses on determining the main phenolic pigments contents and evaluating the solar light collection capacity of ten (10) tinctorial plants crude extracts, via UV-Visible Spectrophotometry. High concentrations of total polyphenols (46.54 mgEqAG/g), flavonoids (10.95 mgEqQuer/g), anthocyanins (181.9 mgL-1 ), and tannins (40.1 mgL-1 ) were recorded in some extracts of these plants. Absorption spectra showed intense absorption domains around 280 nm in the ultraviolet (UV), and also in the visible (400 to 700 nm). These absorptions would be attributed to the π-π* and n-π* electron transitions of the chromophores present in the anthocyanin, flavonoid, carotenoid, and chlorophyll pigments of these plants. The high contents of pigments and the appropriate spectrophotochemical properties make the extracts of these tinctorial plants to become potential raw materials that are useful in solar energy technology.

scholarly journals MULTIFUNCTIONAL BIOLOGICAL PRODUCTS FOR ORGANIC FARMING BASED ON ORGANIC WASTE AND RAW MATERIAL PROCESSING

2021 ◽  
pp. 36-42
Author(s):  
О.А. Оленин ◽  
С.Н. Зудилин
Keyword(s):  
Winter Wheat ◽  
Organic Farming ◽  
Organic Waste ◽  
Raw Materials ◽  
Raw Material ◽  
Organic Fertilizers ◽  
Mineral Fertilizers ◽  
Plant Origin ◽  
Biological Product ◽  
Biological Products

Развитие органического земледелия, в первую очередь, зависит от наличия органических удобрений и биопрепаратов. Цель исследований – на основе утилизации органических отходов и сырья разработать полифункциональные биопрепараты из компонентов животного и растительного происхождения и изучить их влияние на показатели агрофитоценозов и урожайность полевых и садовых культур. В работе использованы результаты исследований по разработке полифункциональных биопрепаратов, а также их эффективности на опытном поле Самарского ГАУ в 2017-2019 гг. В результате была разработана ассортиментная линейка биопрепаратов «АгроТоник» с функциями удобрения и биостимулятора роста: «Цветовод», «Садовод», «Овощевод», «Полевод», «Лесовод» и «Универсальный». «АгроТоник», в отличие от многих органических и минеральных удобрений, содержит все необходимые для растений компоненты: макро-, мезо- и микроэлементы в легкодоступной форме, микроорганизмы, стимуляторы роста – биоактивные фитогормоны, аминокислоты растительного происхождения, витамины, комплекс целлюлозолитических ферментов, почвенные антибиотики, гуминовые вещества, биоприлипатель. Многокомпонентный биопрепарат с функциями удобрения, фунгицида и бактерицида снижал пораженность растений озимой пшеницы на 16,7-27,1 %, по отношению к контролю, и на 17,4-22,6 %, по сравнению с минеральными удобрениями. Биопрепарат повышал урожайность озимой пшеницы на 7,7-25,4 % по сравнению с контролем, тогда как применение пестицида только на 5,3-11,5 %, при стоимости однократно внесенного гербицида в среднем 500,00 руб./га, а биопрепарата – 300,00 руб./га при двукратной обработке. The development of organic farming primarily depends on the availability of organic fertilizers and biological products. The goal of the research is to develop multifunctional biological products from components of animal and plant origin based on the utilization of organic waste and raw materials and to study their impact on the indicators of agrophytocenoses and the yield of field and horticultural crops. The work uses the results of research on the development of multifunctional biological products, as well as their effectiveness in the experimental field of the Samara State Agrarian University in 2017-2019. As a result, the line of "AgroTonik" biological products with the functions of fertilizer and the growth biostimulator was developed: «Tsvetovod», «Sadovod», «Ovoshchevod», «Polevod», «Lesovod» and «Universal'niy». "AgroTonik", unlike many organic and mineral fertilizers, contains all the components necessary for plants: macro -, meso- and microelements in an easily available form, microorganisms, growth stimulators – bioactive phytohormones, amino acids of plant origin, vitamins, a complex of cellulosolytic enzymes, soil antibiotics, humic substances, bio-sticking agent. The multicomponent biological product with the functions of fertilizer, fungicide and bactericide reduced the affection of winter wheat plants by 16.7-27.1 % concerning the control, and by 17.4-22.6 %, in comparison with mineral fertilizers. The biological product increased the yield of winter wheat by 7.7-25.4% compared to the control, while the use of a pesticide only by 5.3-11.5 %, with the cost of a single herbicide on average 500 rubles/ha, and a biological product – 300 rubles/ha with double treatment.

scholarly journals The potentional of plants to cleanup metals from an old landfill site

2017 ◽  
Author(s):  
Yahya Jani ◽  
Charlotte Marchand ◽  
William Hogland
Keyword(s):  
Contaminated Soils ◽  
Hazardous Materials ◽  
Average Concentration ◽  
Soil Samples ◽  
Landfill Site ◽  
Group Company ◽  
Timothy Grass ◽  
Xrf Scanning ◽  
High Concentrations ◽  
The Stability

Old landfill sites contain different hazardous materials like heavy metals which have the ability to affects the entire environment. These places are sometimes covered by plants to increase the stability of the soil and to reduce the effects of erosion. 15 soil samples (3 samples from each place) and 5-7 timothy-grass (Phleum pretense) plants from 5 different places were taken from an old landfill place in an active landfill site in Högbytorp /Sweden owned by Ragn-sells Group Company. XRF scanning was used to analyze the metal content of soil samples and of plants. High concentrations of metals were detected in the soil samples like Fe with an average of about 25000 ppm, Mn about 250 ppm and 2800 ppm of Ti. The plants results showed an average concentration of Fe in the shoots about 730 ppm, Mn about 60 ppm and Ti about 1760 ppm. On the other hand, the roots results showed an average concentration of about 10 000 ppm of Fe, about 160 ppm of Mn and 2200 ppm of Ti. These results gave the indication that the Timothy-grass has the ability to extract metals from contaminated soils and can help to cleanup these soils.

scholarly journals SOIL CONTAMINATION IN PUBLIC SQUARES IN BELO HORIZONTE, MINAS GERAIS, BY CANINE PARASITES IN DIFFERENT DEVELOPMENTAL STAGES

2013 ◽  
Vol 55 (4) ◽  
pp. 229-231 ◽  
Author(s):  
Luciane Madureira Ribeiro ◽  
Ruth Massote Dracz ◽  
Lanuze Rose Mozzer ◽  
Walter dos Santos Lima
Keyword(s):  
Soil Contamination ◽  
Developmental Stages ◽  
Minas Gerais ◽  
Soil Samples ◽  
Fecal Samples ◽  
Belo Horizonte ◽  
The City ◽  
Recreation And Leisure

SUMMARY To evaluate soil contamination by parasites in different developmental stages in public squares used as recreation and leisure areas for children in Belo Horizonte (MG, Brazil), 210 soil samples and 141 canine fecal samples were collected from 42 squares in the city. These samples were analyzed by the Caldwell and Caldwell technique and the Hoffman, Pons, and Janer technique. Of the samples analyzed, 89 (42.4%) soil samples and 104 (73.5%) fecal samples were contaminated with Ancylostoma sp., Toxocara sp., Trichuris sp., or Dipylidium sp. eggs; Giardia sp. cysts; or Isospora sp. oocysts. The commonest parasite was Ancylostoma sp., found in 85% soil and 99% fecal samples, followed by Toxocara sp., found in 43.7% soil and 30.7% fecal samples.

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