scholarly journals Correlation between aerobic and anaerobic microflora of the urogenital tract at dysbiosis in women of Dnipropetrovsk city

2014 ◽  
Vol 5 (2) ◽  
pp. 110-114
Author(s):  
A. H. Yaderna ◽  
L. P. Golodok ◽  
A. I. Vinnikov
Keyword(s):  
Real Time ◽  
Trichomonas Vaginalis ◽  
Mycoplasma Genitalium ◽  
Urogenital Tract ◽  
Gardnerella Vaginalis ◽  
Anaerobic Microorganisms ◽  
Wide Range ◽  
Polymerase Chain ◽  
Further Development ◽  
Atopobium Vaginae

The method of polymerase chain reaction (PCR) in real-time was used to analyze the quantitative characteristics of normal and potentially pathogenic aerobic/facultative-anaerobic and anaerobic biota in the urethra, cervical channel and vagina in healthy women aged 10–40. The biota of all the women under 40 years and some of women older than 40 was mostly represented by lactobacilli. Microbialcomposition of the biocenosis in some women older than 40 is characterized by reduction in quantity of lactobacilli and their replacement by anaerobic microorganisms, mainly, such as Atopobium vaginae (16%), Gardnerella vaginalis (12%), Megasphaera spp. (8%), Dialister spp. (8%), Eubacterium spp. (8%) and Porphyromonas spp. (4%). Rarely, a wide range of other pathogens plays its role, including inter alia: Trichomonas vaginalis (8%), Mycoplasma genitalium (4%), Neisseria gonorrhoeae (2%) andChlamydia trachomatis (1%). The most frequent are the following strain associations: A. vaginae andG. vaginalis,Eubacterium spp. andPorphyromonas spp.,U. (urealyticum + parvum) andM. genitalium,N. gonorrhoeae andCh. trachomatis,U. (urealyticum + parvum) andCandida spp. This is connected with anatomical and physiological characteristics of genitals, hormonal and immune system action. Quantitative study of the biota of urogenital tract in Dnipropetrovsk women with the use of real-time PCR is the sensitive method for diagnosing both physiological and pathological changes, and dysbiotic disorders at early stages and preventing their further development into more serious forms.

scholarly journals Association of Female Genital Schistosomiasis with the Cervicovaginal Microbiota and Sexually Transmitted Infections in Zambian Women

2021 ◽  
Author(s):  
Amy S Sturt ◽  
Emily L Webb ◽  
Lisa Himschoot ◽  
Comfort R Phiri ◽  
Joyce Mapani ◽  
...  
Keyword(s):  
Sexually Transmitted Infections ◽  
Trichomonas Vaginalis ◽  
Mycoplasma Genitalium ◽  
Gardnerella Vaginalis ◽  
Sexually Transmitted ◽  
Weak Evidence ◽  
Key Species ◽  
Atopobium Vaginae ◽  
Female Genital

Abstract Background The cervicovaginal microbiota, including sexually transmitted infections (STI), have not been well-described in female genital schistosomiasis (FGS). Methods Women (aged 18-31, sexually active, non-pregnant) were invited to participate at the final follow-up of HPTN 071 (PopART) Population Cohort in January-August 2018. We measured key species of the cervicovaginal microbiota (Lactobacillus crispatus, L. iners, Gardnerella vaginalis, Atopobium vaginae and Candida) and STI (Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis and Mycoplasma genitalium) using quantitative PCR (qPCR). We evaluated associations of microbiota and STI presence and concentration with FGS (qPCR-detected Schistosoma DNA in any of three genital specimens). Results The presence and concentration of key cervicovaginal species did not differ between participants with (n=30) or without FGS (n=158). A higher proportion of participants with FGS had T. vaginalis compared to FGS negative women (p=0.08), with further analysis showing that T. vaginalis was more prevalent among women with ≥2 Schistosoma qPCR positive genital specimens (50.0%, 8/16) than among FGS negative women (21.5% 34/158, p=0.01). Conclusions We found weak evidence of an association between T. vaginalis presence and FGS, with a stronger association in women with a higher burden FGS infection. Additional research is needed on potential between-parasite interactions, especially regarding HIV-1 vulnerability.

scholarly journals Diagnosis of Trichomonas vaginalis using real-time polymerase chain reaction (PCR) among women at Institut Pasteur of Cte dIvoire

2018 ◽  
Vol 12 (43) ◽  
pp. 988-993
Author(s):  
T. Blavo-Kouamé ◽  
K. E. Angora ◽  
A. Yéo ◽  
A. Ouattara ◽  
A. Ira-Bonouman ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Trichomonas Vaginalis ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Comparative analysis of the rate of microorganism detection in the prostatic fluid and ejaculate using real-time polymerase chain reaction in patients with category IV chronic prostatitis

2020 ◽  
Vol 21 (1) ◽  
pp. 42-48
Author(s):  
D. G. Pochernikov ◽  
V. V. Getman ◽  
N. T. Postovoytenko ◽  
D. M. Rysev ◽  
I. S. Galkina
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Chronic Prostatitis ◽  
Transrectal Ultrasound ◽  
Urogenital Tract ◽  
Significant Heterogeneity ◽  
Chain Reaction ◽  
Study Objective ◽  
Prostatic Fluid ◽  
Polymerase Chain

The study objective is to compare the rate of detection of various microorganisms in the prostatic fluid and ejaculate using real-time polymerase chain reaction in patients with category IV chronic prostatitis.Materials and methods. Between December of 2016 and July 2019, a prospective study including 81 patients with category IV chronic prostatitis per the National Institutes of Health Prostatitis Syndrome Classification (1999) was performed. The patients referred to the clinic of the Ivanovo State Medical Academy for preconception preparation, infertility or erectile disfunction. At the examination, all patients lacked symptoms characteristic of category II or III chronic prostatitis. Transrectal ultrasound of the prostate, microscopic examination of the prostatic fluid and (or) ejaculate, quantitative examination of urogenital tract microbiota using real-time polymerase chain reaction were performed.Results. Comparison of microbiota of the prostatic fluid and ejaculate showed significant differences in the total amount of bacterial mass: in the prostatic fluid mean titer was 3.7 ± 1.6, in the ejaculate it was 2.6 ± 1.8 (p <0.001). Prostatic fluid contained significantly more of the following microorganisms: Enterobacteriaceae spp./Enterococcus spp., Staphylococcus spp., Streptococcus spp., Corynebacterium, Eubacterium, Anaerococcus (p <0.05). No significant differences in the amounts of other microorganisms were observed.Conclusion. The study demonstrates significant heterogeneity of qualitative and quantitative microbiota content in the prostatic fluid and ejaculate. Supposedly, it can be explained by anatomical and physiological characteristics of the prostate, seminal vesicles and periurethral glands that secrete fluid for the ejaculate. The ejaculate contains less microorganisms compared to prostatic fluid which should be taken into account in differential diagnosis of infections of the urogenital tract.The authors declare no conflict of interest.All patients gave written informed consent to participate in the study.

Comparison of Real-Time Polymerase Chain Reaction and Enzyme-Linked Immunosorbent Assay for Sensitive and Quantitative Detection of Hazelnuts in Nut Pastes

2018 ◽  
Vol 101 (6) ◽  
pp. 1864-1867 ◽  
Author(s):  
Ľubica Piknová ◽  
Veronika Janská ◽  
Tomáš Kuchta ◽  
Peter Siekel
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Sandwich Elisa ◽  
Pcr Analysis ◽  
Quantitative Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Chain Reaction ◽  
Wide Range ◽  
Order Of Magnitude ◽  
Polymerase Chain

Abstract Background: Hazelnuts, being a frequent agent of allergenic reactions, need to be detected in food products. Thus, it is necessary to develop and further investigate appropriate methods for detection. Objective: The aim of the study was to compare the analysis of nut pastes (peanut paste spiked with different amounts of hazelnut paste) as a model of contamination of confectionery. Methods: Real-time PCR and sandwich ELISA (RidaScreen Hazelnut Fast Kit) were used. Results: For real-time PCR, LOQ of 2 mg/kg and a quantification range from 2 to 10 000 mg/kg were determined. For ELISA, LOQ of 1 mg/kg and a quantification range from 1 to 100 mg/kg were determined. Conclusions: The comparison shows that the methods had comparable sensitivity with LOQs in the same order of magnitude. Although ELISA was slightly more sensitive, it required dilution of samples at higher concentrations of the analyte because of its narrow quantification range. Results of this study suggest that real-time PCR and ELISA are both suitable methods for the analysis of nut pastes over a wide range of concentrations. Achieved results could be useful for control as well as for technological purposes. Highlights: Real-time PCR analysis of peanut paste spiked with different amounts of hazelnut paste as a model is proposed. Sandwich ELISA analysis of peanut paste spiked with different amounts of hazelnut paste as a model is proposed. The analytical parameters of real-time PCR and ELISA methods are compared.

Prevalence of Mycoplasma genitalium in health clinic attendees complaining of vaginal discharge in Bangladesh

2008 ◽  
Vol 19 (11) ◽  
pp. 772-774 ◽  
Author(s):  
S Rahman ◽  
S Garland ◽  
M Currie ◽  
S N Tabrizi ◽  
M Rahman ◽  
...  
Keyword(s):  
Bacterial Vaginosis ◽  
Trichomonas Vaginalis ◽  
Vaginal Discharge ◽  
Mycoplasma Genitalium ◽  
Health Clinic ◽  
Chain Reaction ◽  
Health Care Clinics ◽  
Primary Health ◽  
Three Samples ◽  
Polymerase Chain

The objective of the study was to determine the prevalence of Mycoplasma genitalium in a sample of health clinic attendees complaining of vaginal discharge. A subsample of 399 vaginal and cervical swabs was randomly selected from 2579 samples collected during a study to determine the causes of vaginal discharge in women attending primary health-care clinics in Dhaka, Bangladesh. Cervical samples were tested for M. genitalium by polymerase chain reaction. In addition, the samples were tested for Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, bacterial vaginosis and candida. M. genitalium was detected in three samples (0.8%; 95% confidence interval: 0.00–1.6). The prevalence of C. trachomatis, N. gonorrhoeae T. vaginalis, bacterial vaginosis and candida was 1.3, 3.8, 8, 23.25 and 32.5%, respectively. Two women with M. genitalium were co-infected with T. vaginalis or candida. This is the first study to document the existence of M. genitalium in Bangladesh. Although the prevalence of this infection is low in the population tested, further research into this pathogen in other Bangladeshi populations is justified.

scholarly journals Effect of the combined preparation Terzhinan® on microorganisms isolated from the urogenital tract of women. In vitro experiment

2017 ◽  
Vol 66 (5) ◽  
pp. 21-26
Author(s):  
Alevtina M. Savicheva ◽  
Elena V. Spasibova
Keyword(s):  
Bacterial Vaginosis ◽  
Streptococcus Agalactiae ◽  
Urogenital Tract ◽  
Gardnerella Vaginalis ◽  
Vitro Experiment ◽  
In Vitro Experiment ◽  
The Family ◽  
Enterococcus Spp ◽  
Atopobium Vaginae

Introduction. The study is in vitro experiment, aimed to determine antibiotic susceptibility of microorganisms isolated from the urogenital tract of women to the combination of substances included in the preparation Terzhinan. Methods. In total, 516 microorganism strains isolated from the vagina of reproductive age women have been tested: Candida spp. (n = 83), bacteria of the family Enterobacteriaceae (n = 138), Streptococcus agalactiae (n = 72), Enterococcus spp. (n = 147), Staphylococcus spp. (n = 37), Actinomyces urogenitalis (n = 7), Gardnerella vaginalis (n = 27), Atopobium vaginae (n = 5). Suspensions of each strain in a volume of 0.5 ml (0.5 according to McFarland) were applied onto culture medium (Muller-Hinton Agar or 5% Blood Muller-Hinton Agar). Furthermore, serial dilutions of Terzhinan were applied with a calibrated loop onto three sectors. Petri dishes were then incubated at 37 °С for 24 h. The results were evaluated visually, by measuring growth inhibition zones. Results. The majority of Candida albicans isolates were susceptible (S) to the preparation, using both undiluted and diluted (10 and 100 times) preparation. Terzhinan without dilution and in the dilution 1:10 was 100% effective against all tested bacteria of the family Enterobacteriaceae. Susceptibility of gram positive bacteria to the antibiotics included in Terzhinan, was 74.1%. All isolates of staphylococci, including Staphylococcus aureus, were susceptible. The frequency of susceptible strains of Streptococcus agalactiae and Enterococcus spp. was 70%. All clinical isolates of Gardnerella vaginalis and Atopobium vaginae were susceptible to the combinations of antibiotics included in the preparation Terzhinan, which is in agreement with data on its high efficiency in the treatment of bacterial vaginosis. Conclusions. In in vitro experiment, the combined preparation Terzhinan showed high activity against major groups of microorganisms isolated from the vagina including pathological conditions, such as bacterial vaginosis and vulvovaginitis of different etiology.

scholarly journals Molecular diagnosis of bacterial vaginosis: Prevalence of Gardnerella vaginalis and Atopobium vaginae in pregnant women

2018 ◽  
Vol 146 (7-8) ◽  
pp. 417-421
Author(s):  
Snezana Matic ◽  
Dane Nenadic ◽  
Jelena Cukic ◽  
Zeljko Mijailovic ◽  
Nevena Manojlovic ◽  
...  
Keyword(s):  
Bacterial Vaginosis ◽  
Pregnant Women ◽  
Real Time ◽  
Vaginal Discharge ◽  
Gardnerella Vaginalis ◽  
Bacterial Number ◽  
Vaginal Smears ◽  
Vaginal Lactobacilli ◽  
Real Time Qpcr ◽  
Atopobium Vaginae

Introduction/Objective. Bacterial vaginosis (BV) is defined as disequilibrium of vaginal microbiota due to proliferation of Gram-negative/variable anaerobes and reduction/depletion of vaginal lactobacilli. Difficulties in interpreting microscopically categorized findings in diagnosis of BV need a molecular analysis of bacteria present in vaginal discharge of patients. In this regard, we performed real-time qPCR analysis of vaginal discharge samples with the goal to explore in which extent prevalence and amount of anaerobes, Gardnerella vaginalis and Atopobium vaginae, are related to findings obtained by microscopy. Methods. This study enrolled 111 asymptomatic pregnant women between 24 and 28 weeks of pregnancy. Gram-stained vaginal smears were evaluated microscopically. Afterwards, DNA of bacteria was extracted from Gram slides and real-time qPCR was performed with the aim to detect and quantify G. vaginalis and A. vaginae. Results. The data of our study showed that 53.2% of patients had normal results, while 20.7% and 26.1% of patients had intermediary (IMD) and BV results, respectively. G. vaginalis and A. vaginae were more frequently found in IMD and BV than in healthy patients; also, the average bacterial number of G. vaginalis and A. vaginae were significantly higher in BV and IMD than in the group with normal findings (p = 0.000). Comparing mutual relation of G. vaginalis and A. vaginae, the prevalence and number of G. vaginalis were in all groups significantly higher than A. vaginae. Conclusion. The data of our study have shown that in distinguishing normal from BV findings, quantification of bacteria may be more important than just molecular detection of bacteria.

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