scholarly journals Закономірності росту фосфатмобілізувальних бактерій у різних типах живильних середовищ

2010 ◽  
Vol 1 (1) ◽  
pp. 78-82
Author(s):  
K. V. Lavrentyeva ◽  
N. V. Cherevach ◽  
A. I. Vinnikov
Keyword(s):  
Pseudomonas Putida ◽  
Growth Pattern ◽  
Physiological Parameters ◽  
Mineral Medium ◽  
Bacterial Cells ◽  
Bacteria Growth ◽  
Soil Phosphate ◽  
Constant Ph

Regularities of two strains of soil phosphate-mobilizing bacteria growth were investigated in different media. Curve of growth and physiological parameters were defined for Pseudomonas putida and Enterobacter dissolvens. Growth pattern of investigated strains was better in the broth medium than in mineral. In these conditions higher concentration of viable bacterial cells was common for E. dissolvens. It was shown that during cultivation pH of mineral medium went down to 4.4-4.5, but broth medium had constant pH 7.0.

scholarly journals Time-Course Proteomic Analysis of Pseudomonas putida KT2440 during Mcl-Polyhydroxyalkanoate Synthesis under Nitrogen Deficiency

Polymers ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 748 ◽  
Author(s):  
Justyna Możejko-Ciesielska ◽  
Agnieszka Mostek
Keyword(s):  
Pseudomonas Putida ◽  
Proteomic Analysis ◽  
Cellular Response ◽  
Time Course ◽  
Molecular Mechanisms ◽  
Nitrogen Deficiency ◽  
Growth Stages ◽  
Bacterial Cells ◽  
Pseudomonas Putida Kt2440 ◽  
Wide Range

Medium-chain-length polyhydroxyalkanoates (mcl-PHAs) have gained great attention as a new green alternative to petrochemical-derived polymers. Due to their outstanding material properties they can be used in a wide range of applications. Pseudomonas putida KT2440 is a metabolically versatile producer of mcl-polyhydroxyalkanoates. Although the metabolism of polyhydroxyalkanoate synthesis by this bacterium has been extensively studied, the comparative proteome analysis from three growth stages of Pseudomonas putida KT2440 cultured with oleic acid during mcl-PHA synthesis has not yet been reported. Therefore; the aim of the study was to compare the proteome of Pseudomonas putida KT2440 at different time points of its cultivation using the 2D difference gel electrophoresis (2D-DIGE) technique. The analyses showed that low levels of a nitrogen source were beneficial for mcl-PHA synthesis. Proteomic analysis revealed that the proteins associated with carbon metabolism were affected by nitrogen starvation and mcl-PHA synthesis. Furthermore, the induction of proteins involved in nitrogen metabolism, ribosome synthesis, and transport was observed, which may be the cellular response to stress related to nitrogen deficiency and mcl-PHA content in bacterial cells. To sum up; this study enabled the investigators to acquire a better knowledge of the molecular mechanisms underlying the induction of polyhydroxyalkanoate synthesis and accumulation in Pseudomonas putida KT2440 that could lead to improved strategies for PHAs in industrial production.

scholarly journals INFLUENCE OF NANOCARBOXYLATES OF MICROELEMENTS ON RHIZOBIUM RADIOBACTER 204 GROWTH-REGULATING ACTIVITY

2015 ◽  
Vol 22 ◽  
pp. 3-8
Author(s):  
С. Ф. Козар ◽  
Є. П. Симоненко ◽  
В. О. Линник ◽  
V. H. Kaplunenko
Keyword(s):  
Growth Phase ◽  
Exponential Growth ◽  
Liquid Culture ◽  
Culture Medium ◽  
Exponential Growth Phase ◽  
Bacterial Cells ◽  
Rhizobium Radiobacter ◽  
Bacteria Growth ◽  
Liquid Culture Medium ◽  
Growth Regulating Activity

The results of studies of the influence of nanocarboxylates (citrates) of microelements on Rhizobium radiobacter 204 growth-regulating activities are presented. It was found that increasing bacteria growth-regulating activity is achieved by the content of citrates of Zinc (Zn), Copper (Cu), Manganese (Mn), Iron (Fe) and Molybdenum (Mo) in culture medium in complex. It was shown that the use of nanocarboxylates of microelements while cultivating the investigated strain contributes to the increase of bacterial cells titre in a liquid culture medium by 1 billion/cm3. It was revealed that the cultivation of R. radiobacter 204 under the action of citrates of microelements helps extent the exponential growth phase of these bacteria by 12 hours. According to the research results an optimized liquid culture medium was proposed for bacteria culturing.

scholarly journals Retarded swarming motility in Bacillus subtilis NRS-762 and Pseudomonas aeruginosa PRD-10

2018 ◽  
Author(s):  
Wenfa Ng
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Bacterial Species ◽  
Nutritional Stress ◽  
Cellular Level ◽  
Bacterial Cells ◽  
Loss Of Function ◽  
Swarming Motility ◽  
Bacteria Growth ◽  
Solid Media

Coping with nutritional stress is essential for cell survival, of which many strategies at the cellular level lend support for ensuring the survival of the population at a particular habitat. One postulated mechanism is swarming motility in bacterial cells, where, upon depletion of nutrients at a locale, cells would coordinate their movement, synthesize more flagella, and secrete lubricants for moving rapidly across surfaces in search for food. Known to engage in swarming motility, Bacillus subtilis and Pseudomonas aeruginosa are two common bacterial species with versatile metabolism that use the motility mode to colonize new habitats with more favourable environmental and nutritional conditions. However, experimental observations of bacteria growth on a variety of agar media revealed that B. subtilis NRS-762 (ATCC 8473) and P. aeruginosa PRD-10 (ATCC 15442) exhibited retarded swarming motility upon entry into stationary phase on solid media. Specifically, B. subtilis NRS-762 colonies exhibited round, wrinkled morphologies compared to complex filamented swarming patterns common in strains able to engage in swarming motility. On the other hand, P. aeruginosa PRD-10 colonies were round, mucoid, and expanded outwards from the colony centre without extending filaments from the centre; thereby, indicating retarded swarming motility. Thus, impaired cellular machinery for swarming motility or mutated and deleted genes likely account for observed retarded swarming motility in B. subtilis NRS-762 and P. aeruginosa PRD-10. More importantly, observations of small filaments extending radially from an expanded colony of P. aeruginosa PRD-10 grown on minimal salts medium supplemented with yeast extract highlighted possible loss of function of effector molecules that transmit cellular decision at swarming motility into movement, while sensory mechanisms feeding into the motility mechanism remained intact. More broadly, observations of impaired swarming motility in B. subtilis NRS-762 and P. aeruginosa PRD-10 in two species otherwise endowed with the motility mode highlighted that additional triggers for swarming motility are likely present, and the motility mode may have been evolutionary selected for other functions in addition to foraging for food in times of nutritional stress.

scholarly journals Karakteristik Bakteri di Perairan Mangrove Pesisir Kraton Pasuruan (Characterization of Bacteria Isolated from Mangrove Coastal Waters of Kraton, Pasuruan)

2014 ◽  
Vol 19 (1) ◽  
pp. 35 ◽  
Author(s):  
Yahya Yahya ◽  
Happy Nursyam ◽  
Yenny Risjani ◽  
Seomarno Soemarno
Keyword(s):  
Pseudomonas Putida ◽  
Lactobacillus Plantarum ◽  
Bacillus Megaterium ◽  
Micrococcus Luteus ◽  
Avicennia Marina ◽  
Bacteria Growth ◽  
Rhizophora Apiculata ◽  
Sonneratia Alba ◽  
Avicennia Alba

Bakteri memainkan peran penting dalam ekosistem mangrove terutama dalam mengurai searasah daun. Keberadaan bakteri ini sangat dipengaruhi oleh berbagai faktor lingkungan. Penelitian ini bertujuan untuk mengisolasi dan karakterisasi bakteri dari perairan mangrove dari dengan vegetasi yang berbeda yakni Avicennia marina, Rhizophora apiculata, Avicennia alba dan Sonneratia alba. Pengambilan sampel dilakukan di perairan mangrove desa Bulu Kerto Kraton Pasuruan. Proses karakterisasi bakteri dilakukan di laboratorium mikrobiologi Fakultas Kedokteran Universitas Brawijaya Malang. Metode penelitian yang digunakan adalah eksploratif sedangkan parameter uji adalah isolasi bakteri dan uji morfologi, biokimia, serta deteksi pertumbuhan bakteri yang dominan. Hasil penelitian diperoleh 7 isolat bakteri Avicennia marina yaitu Nitrococcus sp., B. subtilis, B. pumilus, Pseudomonas putida. P.stutzeri, Micrococcus luteus, Vibrio sp, 6 isolat bakteri Rhizophora apiculata yaitu B. megaterium, Nitrococcus sp, Staphylococcus sp., P.putida, Lactobacillus sp., B. Subtilis,  8 isolat bakteri Avicennia alba yaitu B.megaterium, B.pumilus, B. subtilis, Nitrococcus sp., Pseudomonas putida, P. stutzeri, B. mycoides, Micrococcus sp.. dan 6 isolat bakteri di Sonneratia alba yaitu Bacillus megaterium, Nitrococcus sp., B.subtilis, Planococcus citreus, B. mycoides, Lactobacillus plantarum. Hasil ini menunjukkan bahwa jenis bakteri dipengaruhi oleh vegetasi mangrove yang ada di suatu perairan. Kata kunci: bakteri, perairan mangrove, vegetasi Bacteria play an important role in the ecosystem, especially in degrading mangrove leaves (litter). The presence of these bacteria is greatly influenced by various environmental factors. This research aims to isolate and characterized bacteria from mangrove waters with different vegetaion namely Avicennia marina, Rhizophora apiculata, Avicennia alba and Sonneratia alba. Characterization of the bacteria were done at microbiological laboratory in Medical Faculty, Brawijaya University. Several tests were done in this explorative research i.e. morphological test, biochemistry, and bacteria growth. The results showed there were 7 isolated bacterias found at Avicennia marina area i.e. Nitrococcus sp, B. subtilis, B. pumilus, Pseudomonas putida. P. stutzeri, Micrococcus luteus, Vibrio sp.; 6 isolated bacterias found at  Rhizophora apiculata area i.e. B. megaterium, Nitrococcus sp, Staphylococcus sp., P. putida, Lactobacillus sp., B. subtilis; 8  isolaed bacterias from Avicennia alba area i.e. B. megaterium, B. pumilus, B. subtilis, Nitrococcus sp., Pseudomonas putida, P. stutzeri, B. mycoides, Micrococcus sp., and 6 isolated bacterias from Sonneratia alba area i.e. Bacillus megaterium, Nitrococcus sp, B. subtilis, Planococcus citreus, B. mycoides, Lactobacillus plantarum. These results indicate that the type of bacteria is influenced by the existing mangrove vegetation. Keywords: bacteria, mangrove waters,vegetation

scholarly journals Impacts of Goethite Particles on UV Disinfection of Drinking Water

2005 ◽  
Vol 71 (7) ◽  
pp. 4140-4143 ◽  
Author(s):  
Youxian Wu ◽  
Thomas Clevenger ◽  
Baolin Deng
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Pseudomonas Putida ◽  
Particle Concentration ◽  
Particle Aggregation ◽  
Uv Disinfection ◽  
Bacterial Cells ◽  
Bacterial Inactivation ◽  
Unique Association ◽  
Particle Attachment

ABSTRACT A unique association between bacterial cells and small goethite particles (∼0.2 by 2 μm) protected Escherichia coli and Pseudomonas putida from UV inactivation. The protection increased with the particle concentration in the turbidity range of 1 to 50 nephelometric turbidity units and with the bacterium-particle attachment time prior to UV irradiation. The lower degree of bacterial inactivation at longer attachment time was mostly attributed to the particle aggregation surrounding bacteria that provided shielding from UV radiation.

scholarly journals Influence of temperature on the growth of Pseudomonas putida

2016 ◽  
Vol 5 (1) ◽  
pp. 9-12 ◽  
Author(s):  
Md Sakil Munna ◽  
Zebunnesa Zeba ◽  
Rashed Noor
Keyword(s):  
Pseudomonas Putida ◽  
Morphological Changes ◽  
Bacterial Cells ◽  
Bacterial Physiology ◽  
Colony Forming Units ◽  
Different Temperatures ◽  
Influence Of Temperature On ◽  
Agar Media ◽  
C 30 ◽  
Lower Temperature

Lots of reports on the generation of stress by increase in temperature in the bacterial cells especially in Escherichia coli has been observed so far. Current study further emphasized such effect on the cells of Pseudomonas putida (SUBP03). Conventional methods relating growth assessment of bacteria were employed. The optical density of bacterial cells at 600 nm (OD600) in the minimal broth along with the culturable cells were assessed in the form of colony forming units (CFUs) in the minimal agar media at different temperatures (27 °C, 30 °C, 33 °C, 37 °C and 40 °C). Morphological observations were made to further clarify the bacterial physiology and the spot tests were performed to examine the cell viability. Cells of P. putida (SUBP03) were found to grow vigorously at 30 °C, while the growth was found to decline at lower temperature (27 °C) and along with the increase in temperature (at 33 °C, 37 °C and 40 °C). However, the morphological changes were insignificant. Furthermore, cells were noticed to completely lose culturability at 40 °C after 48 hours.Stamford Journal of Microbiology, Vol.5(1) 2015: 9-12

Purification and characterization of adhesive exopolysaccharides from Pseudomonas putida and Pseudomonas fluorescens

1987 ◽  
Vol 33 (12) ◽  
pp. 1080-1090 ◽  
Author(s):  
R. R. Read ◽  
J. W. Costerton
Keyword(s):  
Electron Microscopy ◽  
Chemical Composition ◽  
Carbon Source ◽  
Pseudomonas Putida ◽  
Solid Surface ◽  
Extracellular Polysaccharide ◽  
Variable Degree ◽  
Bacterial Cells ◽  
Purification And Characterization

In this study, the adhesive exopolysaccharides of strains of Pseudomonas putida and P. fluorescens, both isolated from freshwater epilithic communities, were examined with regard to their chemical composition, biosynthesis, and their role in adhesion. Electron microscopy showed that both strains were enrobed in fibrous glycocalyces and that these structures were involved in attachment of the cells to a solid surface and as structural matrices in the microcolony mode of growth. In batch culture experiments most of the extracellular polysaccharide of both strains was found to be soluble in the growth medium rather than being associated with bacterial cells. Exopolysaccharide was synthesized during all phases of growth, but when growth was limited by exhaustion of the carbon source, exopolysaccharide synthesis ceased whereas exopolysaccharide synthesis continued for some time after cessation of growth in nitrogen-limited cultures. Exopolysaccharide from both strains was isolated and purified. Pseudomonas putida synthesized an exopolysaccharide composed of glucose, galactose, and pyruvate in a ratio of 1:1:1; the P. fluorescens polymer contained glucose, galactose, and pyruvate in a ratio of 1:1:0.5, respectively. Polymers from both strains were acetylated to a variable degree.

scholarly journals Retarded swarming motility in Bacillus subtilis NRS-762 and Pseudomonas aeruginosa PRD-10

2018 ◽  
Author(s):  
Wenfa Ng
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Bacterial Species ◽  
Nutritional Stress ◽  
Cellular Level ◽  
Bacterial Cells ◽  
Loss Of Function ◽  
Swarming Motility ◽  
Bacteria Growth ◽  
Solid Media

Coping with nutritional stress is essential for cell survival, of which many strategies at the cellular level lend support for ensuring the survival of the population at a particular habitat. One postulated mechanism is swarming motility in bacterial cells, where, upon depletion of nutrients at a locale, cells would coordinate their movement, synthesize more flagella, and secrete lubricants for moving rapidly across surfaces in search for food. Known to engage in swarming motility, Bacillus subtilis and Pseudomonas aeruginosa are two common bacterial species with versatile metabolism that use the motility mode to colonize new habitats with more favourable environmental and nutritional conditions. However, experimental observations of bacteria growth on a variety of agar media revealed that B. subtilis NRS-762 (ATCC 8473) and P. aeruginosa PRD-10 (ATCC 15442) exhibited retarded swarming motility upon entry into stationary phase on solid media. Specifically, B. subtilis NRS-762 colonies exhibited round, wrinkled morphologies compared to complex filamented swarming patterns common in strains able to engage in swarming motility. On the other hand, P. aeruginosa PRD-10 colonies were round, mucoid, and expanded outwards from the colony centre without extending filaments from the centre; thereby, indicating retarded swarming motility. Thus, impaired cellular machinery for swarming motility or mutated and deleted genes likely account for observed retarded swarming motility in B. subtilis NRS-762 and P. aeruginosa PRD-10. More importantly, observations of small filaments extending radially from an expanded colony of P. aeruginosa PRD-10 grown on minimal salts medium supplemented with yeast extract highlighted possible loss of function of effector molecules that transmit cellular decision at swarming motility into movement, while sensory mechanisms feeding into the motility mechanism remained intact. More broadly, observations of impaired swarming motility in B. subtilis NRS-762 and P. aeruginosa PRD-10 in two species otherwise endowed with the motility mode highlighted that additional triggers for swarming motility are likely present, and the motility mode may have been evolutionary selected for other functions in addition to foraging for food in times of nutritional stress.

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