scholarly journals Ecology of zoophilic flies in livestock biocenoses of Ukraine

2021 ◽  
Vol 29 (3) ◽  
pp. 258-263
Author(s):  
A. P. Paliy ◽  
A. N. Mashkey ◽  
L. I. Faly ◽  
O. S. Kysterna ◽  
H. I. Rebenko ◽  
...  
Keyword(s):  
Nutrient Medium ◽  
Animal Husbandry ◽  
Fold Increase ◽  
Biological Properties ◽  
House Fly ◽  
Stomoxys Calcitrans ◽  
Parasitic Diseases ◽  
Phase Development ◽  
Intraspecies Competition ◽  
High Degree

In modern animal husbandry of Ukraine, there are still some unresolved issues related to the high number of zoophilic flies in the territories of facilities and the spread of animal infectious and parasitic diseases. A detailed study of bioecological peculiarities of the dominant zoophilic fly species contributes to improving the effectiveness of measures to control ectoparasites. 27 zoophilic fly species have been found in animal breeding complexes. The maximum number of parasitic Diptera species was recorded on cattle-keeping premises. The biological properties of Neomyia cornicina (Fabricius, 1781) (size, shape, colour, duration of preimaginal phase development) were studied. Also, we studied the dynamics of the number and daily activity of dominant fly species (Musca domestica Linnaeus, 1758, M. autumnalis De Geer, 1776, Stomoxys calcitrans (Linnaeus, 1758)). When studying the intraspecies competition, a high-degree survival of M. domestica and M. autumnalis was determined in the conditions of critical nutritional deficiency (0.5 g of nutrient medium per larva) and increased density of individuals (the imago emergence was 38.6% and 34.0%, respectively). In similar maintenance conditions, the emergence of N. cornicina imago was low (14.6%). With a two-fold increase in the insectarium volume and in the amount of nutrient medium (1 g per larva), the imago emergence of M. domestica, M. autumnalis and N. cornicina increased to 64.0%, 39.2%, and 24.0%, respectively. With an even greater increase in the amount of nutrient medium (2 g per larva), the maximum emergence of imagoes of all the studied fly species was observed (M. domestica, M. autumnalis, and N. cornicina: 96.6%, 91.2% and 72.6%, respectively). In the conditions of interspecific competition, M. autumnalis suppressed N. cornicina even in conditions of a sufficient amount of nutrient substrate. In the competition between M. domestica and M. autumnalis, house fly dominated. Increasing the nutrient medium volume narrowed the gap between the competing species.

scholarly journals Evolution of dependoparvoviruses across geological timescales—implications for design of AAV-based gene therapy vectors

2020 ◽  
Vol 6 (2) ◽  
Author(s):  
Evin Hildebrandt ◽  
Judit J Penzes ◽  
Robert J Gifford ◽  
Mavis Agbandje-Mckenna ◽  
Robert M Kotin
Keyword(s):  
Gene Therapy ◽  
In Silico Analysis ◽  
Biological Properties ◽  
Variable Regions ◽  
Evolution Of Viruses ◽  
Extant Species ◽  
Gene Therapy Vectors ◽  
Endogenous Viral Elements ◽  
High Degree ◽  
A2 Domain

Abstract Endogenous viral elements (EVEs) are genetic remnants of viruses that have integrated into host genomes millions of years ago and retained as heritable elements passed on to offspring until present-day. As a result, EVEs provide an opportunity to analyse the genomes of extinct viruses utilizing these genomic viral fossils to study evolution of viruses over large timescales. Analysis of sequences from near full-length EVEs of dependoparvoviral origin identified within three mammalian taxa, Whippomorpha (whales and hippos), Vespertilionidae (smooth-nosed bats), and Lagomorpha (rabbits, hares, and pikas), indicates that distinct ancestral dependoparvovirus species integrated into these host genomes approximately 77 to 23 million years ago. These ancestral viruses are unique relative to modern adeno-associated viruses (AAVs), and distinct from extant species of genus Dependoparvovirus. These EVE sequences show characteristics previously unseen in modern, mammalian AAVs, but instead appear more similar to the more primitive, autonomously replicating and pathogenic waterfowl dependoparvoviruses. Phylogeny reconstruction suggests that the whippomorph EVE orthologue derives from exogenous ancestors of autonomous and highly pathogenic dependoparvovirus lineages, believed to have uniquely co-evolved with waterfowl birds to present date. In contrast, ancestors of the two other mammalian orthologues (Lagomorpha and Vespertilionidae) likely shared the same lineage as all other known mammalian exogenous AAVs. Comparative in silico analysis of the EVE genomes revealed remarkable overall conservation of AAV rep and cap genes, despite millions of years of integration within the host germline. Modelling these proteins identified unexpected variety, even between orthologues, in previously defined capsid viral protein (VP) variable regions, especially in those related to the three- and fivefold symmetry axes of the capsid. Moreover, the normally well-conserved phospholipase A2 domain of the predicted minor VP1 also exhibited a high degree of sequence variance. These findings may indicate unique biological properties for these virus ‘fossils’ relative to extant dependoparvoviruses and suggest key regions to explore within capsid sequences that may confer novel properties for engineered gene therapy vectors based on paleovirology data.

Field trials of Trichomalopsis sarcophagae (Hymenoptera: Pteromalidae) in cattle feedlots: a potential biocontrol agent of filth flies (Diptera: Muscidae)

2003 ◽  
Vol 135 (4) ◽  
pp. 599-608 ◽  
Author(s):  
K.D. Floate
Keyword(s):  
Biocontrol Agent ◽  
Natural Populations ◽  
Fold Increase ◽  
Mass Rearing ◽  
Field Trials ◽  
House Fly ◽  
Stable Fly ◽  
Large Numbers ◽  
Potential Biocontrol Agent ◽  
Paired Control

AbstractA field study was performed in southern Alberta, Canada, to assess the native wasp, Trichomalopsis sarcophagae (Gahan), as a potential biocontrol agent for house fly, Musca domestica L., and stable fly, Stomoxys calcitrans (L.). The wasp was readily reared in large numbers, which allowed for the cumulative release of an estimated 4.63 million wasps into three commercial feedlots during the 2-year study. Each of several releases predictably and repeatedly enhanced parasitism of sentinel house fly pupae, whereas parasitism remained low in three paired control feedlots where wasps were not released. Releases every 2nd week had a disproportionately greater effect than releases every 2nd month. In 1998, 1.2 million wasps were released into treatment feedlots resulting in the recovery of 3 952 T. sarcophagae from 31 500 sentinel pupae (0.13 wasps/pupa). In 1999, 3.43 million wasps were released into treatment feedlots, with the recovery of 37 763 wasps from 47 720 sentinel pupae (0.79 wasps/pupa). Hence, a 2.8-fold increase in the number of wasps released in 1999 resulted in a 6.1-fold increase in the recovery of wasps. This result supports industry recommendations of regular, repeated releases of wasps every 2nd or 4th week versus one or infrequent releases throughout the summer. There was no evidence that releases augmented overwintering populations of the wasp in subsequent years. These results provide proof-of-concept for the mass-rearing and release of T. sarcophagae as an inundative biocontrol agent for the control of pest flies in cattle confinements. Further studies will be required to assess the effect of T. sarcophagae releases on natural populations of pest flies.

Accommodative ability in patients with strabismic amblyopia at concomitant strabismus

2020 ◽  
Vol 26 (1) ◽  
pp. 16-20
Author(s):  
Valentina A. Usenko ◽  
Aizhamal I. Berdibaeva
Keyword(s):  
Visual Acuity ◽  
Fold Increase ◽  
Ciliary Muscle ◽  
Control Group ◽  
Healthy Individuals ◽  
Strabismic Amblyopia ◽  
Distance Visual Acuity ◽  
Before And After ◽  
Concomitant Strabismus ◽  
High Degree

Aim. To study the state of the accommodative ability of amblyopia before and after treatment. Materials and methods. One hundred patients (158 eyes) with friendly alternating and monolateral strabismus were examined. The control group consisted of 10 healthy individuals (20 eyes) with emmetropia: the average age of patients under 3 years of age was 2.4 0.15 years, from 3 years to 7 years, 4.8 0.07 years, and above 7 years, 8.3 0.4 years. The vast majority of patients (84 people, 126 eyes) had hyperopic astigmatism, and 16 patients (32 eyes) had a high degree of hyperopia. Twenty-eight patients (40 eyes), 48 patients (84 eyes), and 20 patients (26 eyes) had amblyopia of the 1st, 2nd, and 3rd degrees, respectively. Along with generally accepted methods for examining eyes, the OAA volume and ZAA were determined as the absolute accommodation margin by the proximetric method (AKA-0.1) before and after hardware treatment to relax and stimulate the ciliary muscle. Results. The results after treatment revealed a 1.52-fold increase in OAA to 14.4 0.23 D; 13.5 0.10 D; 13.1 0.41 D; and ZAA and a significant 2-fold increase in visual acuity (P 0.001). Conclusion. A criterion for the effectiveness of hardware treatment for dysbinocular amblyopia is an increase in OAA and ZAA, accompanied by a 2-fold increase in distance visual acuity (P 0.001).

Development micromachined cyber platforms to cultive endothelial сapillary networks in vitro in the space organized microflows nutrient medium

2015 ◽  
Vol 9 (2) ◽  
pp. 0-0
Author(s):  
Найденов ◽  
E. Naydenov
Keyword(s):  
Nutrient Medium ◽  
Life Support ◽  
Three Dimensional ◽  
Biological Properties ◽  
Special Equipment ◽  
Mathematical Functions ◽  
Capillary Networks ◽  
Digital Control Systems ◽  
Long Time

This work is devoted to the development of technology and special equipment for the cultivation of spontaneously developing functioning endothelial capillary networks in vitro as the basis of artificial cloth-like structures with desired biological properties. It is the scientific and engineering projects RFBR №94-04-13544 «Structural analysis of microvascular bifurcations" and №96-04-50991 «Cell and Tissue Engineering endothelium (formation in endothelial culture in vitro the functioning self-developing capillary networks)." The proposed technology allows the author to form three-dimensional capillary endothelial network around micro-fluidic arrays, immersed in a specially designed dynamic gel. In 2013, the Korean research team under the lea-dership Noo Li Jeon has reproduced, using a similar approach, the phenomenon of self-developing functioning endothelial capillary networks with mass transfer in vitro. It has fully confirmed the validity of the concept pro-posed in the listed projects. Using system of the mathematical modeling Matlab & Simulink and system engi-neering design Cadence Orcad it was developed simulation mathematical model and circuit diagrams experimental reactor modules, it allows to saving considerable financial resources allocated to research and de-velopment of this kind. The resulting model contains 5.4 million basic Simulink blocks and performs more than 7,000 different mathematical functions, reflecting the behavior of devices in stationary and non-stationary conditions. Device control is based on neural network technology. Portable stand-alone microcomputers cyber platform includes microfluidic matrix, generators of microflows liquid phase nutrient medium, life-support systems of endothelial culture system of automatic digital imaging process of angiogenesis, the transmission system of encrypted data over a secure radio, digital control systems. All systems are backed up multiple times, allowing the product to operate in stand-alone mode for a long time (up to a year or more).

Gill membrane remodeling with soft-water acclimation in zebrafish (Danio rerio)

2007 ◽  
Vol 30 (1) ◽  
pp. 53-60 ◽  
Author(s):  
Paul M. Craig ◽  
Chris M. Wood ◽  
Grant B. McClelland
Keyword(s):  
Danio Rerio ◽  
Metal Toxicity ◽  
Model Organism ◽  
Fold Increase ◽  
Soft Water ◽  
Membrane Remodeling ◽  
Protein Levels ◽  
High Degree ◽  
Carbonic Anhydrase 1 ◽  
Mrna Expression Levels

Little is known regarding the ionoregulatory abilities of zebrafish exposed to soft water despite the popularity of this model organism for physiology and aquatic toxicology. We examined genomic and nongenomic changes to gills of zebrafish as they were progressively acclimated from moderately hard freshwater to typical soft water over 7 days and held in soft water for another 7 days. Gills were sampled daily and mRNA expression levels of gill Na+-K+-ATPase (NKA) α1a subunit, epithelium calcium channel (ECaC), carbonic anhydrase-1 and 2 (CA-1, CA-2), Na+/H+ exchanger (NHE-2), V-type proton (H+)-ATPase, and copper transport protein (CTR-1) were quantified by real-time PCR. Changes in enzyme activities of gill NKA were determined and protein levels of NKA and ECaC were quantified by Western blotting. Levels of mRNA for ECaC increased fourfold after day 6, with an associated increase in ECaC protein levels after 1 wk in soft water. CA-1 and CA-2 exhibited a 1.5- and 6-fold increase in gene expression on days 6 and 5, respectively. Likewise, there was a fivefold increase in NHE-2 expression after day 6. Surprisingly, CTR-1 mRNA showed a large transient increase (over threefold) on day 6, while H+-ATPase mRNA did not change. These data demonstrate a high degree of phenotypic plasticity in zebrafish gills exposed to an ion-poor environment. This not only enhances our understanding of ionoregulatory processes in fish but also highlights the need for proper experimental design for studies involving preacclimation to soft water (e.g., metal toxicity).

SURVEY AND RELEASE OF PARASITOIDS (HYMENOPTERA) ATTACKING HOUSE AND STABLE FLIES (DIPTERA: MUSCIDAE) IN DAIRY OPERATIONS

1999 ◽  
Vol 131 (6) ◽  
pp. 743-756 ◽  
Author(s):  
Tanja McKay ◽  
Terry D. Galloway
Keyword(s):  
House Fly ◽  
Stomoxys Calcitrans ◽  
Stable Fly ◽  
Nasonia Vitripennis ◽  
Pupal Parasitoid ◽  
House Flies ◽  
Stable Flies ◽  
Naturally Occurring ◽  
The Status ◽  
No Releases

AbstractIn 1995, Nasonia vitripennis (Walker) (Hymenoptera: Pteromalidae), a commercially available pupal parasitoid of the house fly, Musca domestica L., and stable fly, Stomoxys calcitrans (L.) (Diptera: Muscidae), was purchased to examine the status of wasps being sold to Manitoba producers. Percentage of pupae parasitized, numbers of parasitoids per pupa, total parasitoids, and parasitoid sex ratio were determined for each shipment of parasitoids received. To determine the extent to which these wasps could successfully parasitize house flies and stable flies, parasitoids were released weekly in four Manitoba dairy barns and levels of parasitism estimated. In 10 622 freeze-killed sentinel house fly pupae, 2.2% were parasitized throughout the season by N. vitripennis, and 5.8% were parasitized by eight other species of parasitoids. Of 11 897 naturally occurring house fly and stable fly pupae, 0.6% were parasitized by N. vitripennis, and 3.4% by eight other species of parasitoids. In four barns where there were no releases of N. vitripennis, 1.1% of 11 779 sentinel pupae were parasitized by four species of parasitoids and 3.8% of 8384 naturally occurring house fly and stable fly pupae were parasitized by nine species. The release of an estimated 3 648 093 N. vitripennis did not result in substantial parasitism in either sentinel pupae or naturally occurring pupae. In 1996, live sentinel house fly pupae (n = 50 842) and house fly and stable fly pupae occurring naturally (n = 4691) were collected in two of the nonrelease barns from the 1995 study to examine the activity of endemic parasitoids. Of the sentinel and naturally occurring pupae sampled, 4.0% and 9.4% were parasitized, respectively. Phygadeuon fumator Gravenhörst (Hymenoptera: Ichneumonidae) was the most abundant parasitoid, accounting for 97.4% and 79.9% of parasitoids collected from sentinel pupae and naturally occurring pupae, respectively. Other parasitoids included Urolepis rufipes (Ashmead), Muscidifurax raptor Girault and Sanders, Muscidifurax zaraptor Kogan and Legner, Spalangia subpunctata Först, Spalangia cameroni Perkins, Spalangia nigra Latreille, and a species of Trichomalopsis Crawford (Hymenoptera: Pteromalidae).

scholarly journals Analysis and Characterization of the Complete Genome of Tupaia (Tree Shrew) Herpesvirus

2001 ◽  
Vol 75 (10) ◽  
pp. 4854-4870 ◽  
Author(s):  
Udo Bahr ◽  
Gholamreza Darai
Keyword(s):  
Dna Sequences ◽  
Tree Shrew ◽  
Biological Properties ◽  
Open Reading Frames ◽  
Murine Cytomegalovirus ◽  
Unique Region ◽  
Viral Genes ◽  
Conserved Genes ◽  
Dna Strands ◽  
High Degree

ABSTRACT The tupaia herpesvirus (THV) was isolated from spontaneously degenerating tissue cultures of malignant lymphoma, lung, and spleen cell cultures of tree shrews (Tupaia spp.). The determination of the complete nucleotide sequence of the THV strain 2 genome resulted in a 195,857-bp-long, linear DNA molecule with a G+C content of 66.5%. The terminal regions of the THV genome and the loci of conserved viral genes were found to be G+C richer. Furthermore, no large repetitive DNA sequences could be identified. This is in agreement with the previous classification of THV as the prototype species of herpesvirus genome group F. The search for potential coding regions resulted in the identification of 158 open reading frames (ORFs) regularly distributed on both DNA strands. Seventy-six out of the 158 ORFs code for proteins that are significantly homologous to known herpesvirus proteins. The highest homologies found were to primate and rodent cytomegaloviruses. Biological properties, protein homologies, the arrangement of conserved viral genes, and phylogenetic analysis revealed that THV is a member of the subfamilyBetaherpesvirinae. The evolutionary lineages of THV and the cytomegaloviruses seem to have branched off from a common ancestor. In addition, it was found that the arrangements of conserved genes of THV and murine cytomegalovirus strain Smith, both of which are not able to form genomic isomers, are colinear with two different human cytomegalovirus (HCMV) strain AD169 genomic isomers that differ from each other in the orientation of the long unique region. The biological properties and the high degree of relatedness of THV to the mammalian cytomegaloviruses allow the consideration of THV as a model system for investigation of HCMV pathogenicity.

scholarly journals Safety profiling of genetically engineered Pim-1 kinase overexpression for oncogenicity risk in human c-kit+ cardiac interstitial cells

2019 ◽  
Author(s):  
Kathleen Broughton ◽  
Kelli Korski ◽  
Oscar Echeagaray ◽  
Robert Adamson ◽  
Walter Dembitsky ◽  
...  
Keyword(s):  
Stem Cell ◽  
Kinase Activity ◽  
Transcriptome Profiling ◽  
Fold Increase ◽  
Soft Agar ◽  
Interstitial Cells ◽  
Biological Properties ◽  
Genetically Engineered ◽  
Therapeutic Interventions ◽  
Myocardial Repair

AbstractAdvanced approaches to stem cell-based therapies is necessary for myocardial regenerative therapy because treatments have yielded modest results in the clinic. Our group previously demonstrated genetic modification of cardiac stem cells with Pim-1 kinase overexpression rejuvenated aged cells and potentiated myocardial repair. Despite these encouraging findings, concerns were raised regarding oncogenic risk associated with Pim-1 kinase overexpression. Testing of these c-kit+ cardiac interstitial cells (cCICs), derived from heart failure patient samples, overexpressing Pim-1 (cCICs-Pim-1) for indices of oncogenic risk was assessed by soft agar colony formation, micronucleation, gamma-Histone 2AX foci, and transcriptome profiling. Collectively, findings demonstrate comparable phenotypic and biological properties of cCICsPim-1 compared to baseline control cCICs with no evidence for oncogenic phenotype. Using a highly-selective and continuous sensor for quantitative assessment of PIM1 kinase activity, a 7-fold increase in cCICs-Pim-1 versus cCICs resulted. Kinase activity was elevated in IKKs, AKT/SGK, CDK1-3, p38, and ERK1/2 in addition to Pim-1, correlating Pim-1 overexpression to contribute to Pim-1-mediated effects. Enhancement of cellular survival, proliferation, and other beneficial properties to augment stem cell-mediated repair without oncogenic risk is a feasible, logical, and safe approach to improve efficacy and overcome current limitations inherent to cellular adoptive transfer therapeutic interventions.

scholarly journals PARASITES AND PARASITIC DISEASES IN DOMESTIC REINDEER OF YAKUTIA

2021 ◽  
pp. 254-260
Author(s):  
Kokolova ◽  
Gavrilieva ◽  
Stepanova ◽  
Dulova ◽  
Sivtseva
Keyword(s):  
Far East ◽  
Animal Husbandry ◽  
Parasitic Diseases ◽  
Way Of Life ◽  
Reindeer Husbandry ◽  
Cultural Traditions ◽  
The North ◽  
Significant Damage ◽  
The Republic ◽  
Further Development

Reindeer husbandry is the basis of the economy and way of life, the traditional occupation of the indigenous inhabitants of the North, Siberia and the Far East, and the most important branch of animal husbandry in the Republic of Sakha (Yakutia). Reindeer husbandry is associated with the cultural traditions, lifestyle and economy of the small national communities of the north. Yakutia is the traditional centre and the region of breeding domestic reindeer. Further development of reindeer husbandry, and increasing of the productivity and profitability of reindeer husbandry in Yakutia are impossible without proper organization and effective protection of domestic reindeer from various diseases, including parasitic ones, which cause significant damage to reindeer farms in the Republic. Reindeer are infected with 37 species of helminths. The results of the research of the authors of the article showed that all the studied deer had different types of helminths, and 100% were infected with gadfly larvae. The most frequent parasitic diseases in deer are monieziasis, larval echinococcosis, parenchymal, muscular and serosal cysticercosis, dictyocaulosis, nematodirellosis and elaphostrongylosis; moreover other parasitoses – edemagenosis and cephenomyosis – are recorded in 100% of deer. The authors of the article consider the infection of deer with larvae of subcutaneous and nasopharyngeal gadflies to be the number one problem.

scholarly journals Kualitas Air pada DAS Bugis dan DAS Wain di Kawasan Hutan Lindung Sungai Wain Balikpapan

2020 ◽  
Vol 4 (2) ◽  
pp. 77
Author(s):  
Sri Sarminah ◽  
Dyna Raya Anugerah ◽  
Marlon Ivanhoe Aipassa ◽  
Agus Din
Keyword(s):  
Water Quality ◽  
River Basin ◽  
Government Regulation ◽  
Agricultural Waste ◽  
Animal Husbandry ◽  
Fish Farming ◽  
Quality Standards ◽  
Biological Properties ◽  
Regional Government ◽  
Water Quality Standards

 This study aims to determine the latest water quality conditions in terms of physical, chemical and biological properties in the DAS Bugis and DAS Wain. Analysis of water sample was conducted at the Water Quality Laboratory of the Department of Aquatic Resources and Fisheries and Marine Sciences, Mulawarman University, Samarinda. Data analysis refers to the Water Quality Standards based on East Kalimantan Regional Government Regulation No. 02/2011 about Management of Water Quality and Water Pollution Control. The results showed that the parameters of temperature, colour, TSS, TDS, BOD5, SO4 and NO3 were included in the Class I water quality standards that can be used as drinking water or for other consumption purposes. COD parameters (in the Right River Basin), DO and NH3 (in the River Basin) were included in Class II water quality standards, which can be used for water recreation infrastructure / facilities, freshwater fish farming, animal husbandry and plants irrigation. The pH parameters in the DAS Bugis were included in class IV water quality standards that can only be used to irrigate plants. Pollutant loads that contribute a lot to enter were agricultural and agricultural waste and domestic waste originating from community forest areas.

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