scholarly journals The biology of Simulium noelleri and Simulium dolini: morphological, ecological and molecular data

2021 ◽  
Vol 29 (2) ◽  
pp. 180-184
Author(s):  
M. O. Zinchenko ◽  
K. B. Sukhomlin ◽  
O. P. Zinchenko ◽  
V. S. Tepliuk
Keyword(s):  
Genetic Difference ◽  
Molecular Genetic ◽  
Genetic Research ◽  
Morphological Characteristics ◽  
Taxonomic Status ◽  
Molecular Data ◽  
Species Group ◽  
Small Rivers ◽  
Genetic Characteristics ◽  
First Time

Molecular genetic research has revolutionized the taxonomy and systematics of the Simuliidae family. Simulium noelleri Friederichs, 1920 is a species of blackfly, common in the Holarctic, reported for 33 countries. In 1954, Topchiev recorded it in Ukraine for the first time. Simulium dolini Usova et Sukhomlin, 1989 has been recorded at the borders of Ukraine and Belarus. It was described for the first time by Usova and Sukhomlin in 1989 from the collection from the territory of Volyn region in 1985. Usova and Sukhomlin, Yankovsky, Adler state that S. noelleri and S. dolini are different species by the morphological characteristics that differ in all phases of development. Adults differ in the structure of the genital appendages, palps, the margin and shape of the face and forehead, the colour of the legs; the larva – in the pattern on the frontal capsule, the number of rays in the fans, mandibular teeth and the hypostoma, the structure of the hind organ of attachment; pupae – in the branching way of gills. Molecular data are becoming an increasingly important tool in insect taxonomy. Therefore, we had to check that these two closely related species also have genetic difference. The development of S. noelleri and S. dolini was studied in four small rivers of Volyn region, Ukraine (Chornohuzka, Konopelka, Putylivka, Omelyanivka) in the period from 2017 to 2019. During initial processing of insect samples, we used the standard protocols EPPO PM7/129. We obtained the nucleotide sequence of S. dolini. It was proved that the populations of S. noelleri and S. dolini from medium and small rivers of Volyn differ in biological, morphological, behavioural and genetic characteristics. Comparison of the species S. noelleri with the data of the GenBank confirms the identification of three distinct morphotypes from Volyn, Great Britain and Canada. As a result of the conducted researches, it was confirmed that two close species of S. dolini and S. noelleri from the noelleri species group differ in the structure of mitochondrial DNA, which confirms their independent taxonomic status. Additional studies comprising more individuals from larger areas of Europe are required to verify the taxonomic position of these two species.

Molecular genetics and phylogeny of Ephippigera species group of genus Saga Charpentier, 1825 (Orthoptera: Ensifera: Saginae) in Anatolia

Zootaxa ◽  
2021 ◽  
Vol 4991 (2) ◽  
pp. 331-342
Author(s):  
BURCU BİNİCİ ◽  
DENİZ ŞİRİN ◽  
MEHMET SAİT TAYLAN
Keyword(s):  
16S Rdna ◽  
Molecular Genetic ◽  
Genetic Research ◽  
Molecular Data ◽  
Species Group ◽  
Middle Pleistocene ◽  
Gene Sequences ◽  
16S Rdna Gene ◽  
Chain Reactions ◽  
Rdna Gene

The genus Saga, one of the largest predatory bush crickets and insects in the Palearctic region, is represented by nine species in Anatolia. According to the morphological and bioacoustics evaluations of the Ephippigera species group belonging to the Saga genus, it consists of three species: S. ephippigera, S. syriaca, and S. hakkarica. To the present, however, there is no molecular genetic research on this species group. In our study, by using the 16S rDNA gene sequences, the molecular phylogeny of this species group is revealed and the morphological species hypotheses has been tested. In the study, specimens belonging to S. ephippigera, S. syriaca, and S. hakkarica were collected between the years of 2018 and 2020 with sweep netting; the DNA isolation, polymerase chain reactions, purifications and double-stranded sequencing were performed to obtain 16S rDNA gene sequences. Sequences were evaluated phylogenetically by performing alignment, phylogenetic analysis (Maximum Likelihood and Bayesian Inference), and dating of cladogenetic events, respectively. The monophyletic and species hypotheses of all three species have been supported by molecular data. It has been determined that the S. ephippigera species is the origin lineages of the Ephippigera species group which diverged approximately 0.4 million years ago; it has been also revealed that the climatic changes in the glacial and inter-glacial periods in the Middle Pleistocene period affected greatly the evolution of the species group.  

scholarly journals Clinical, hormonal and molecular-genetic characteristics of monogenic diabetes mellitus associated with the mutations in the INS gene

2022 ◽  
Vol 24 (5) ◽  
pp. 414-421
Author(s):  
Yu. V. Tikhonovich ◽  
E. E. Petryaykina ◽  
A. V. Timofeev ◽  
N. A. Zubkova ◽  
A. A. Kolodkina ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Russian Federation ◽  
Clinical Characteristics ◽  
Molecular Genetic ◽  
Genetic Research ◽  
Gene Mutations ◽  
Insulin Biosynthesis ◽  
Coding Region ◽  
Genetic Characteristics ◽  
First Time

Background: Currently more than 50 mutations of the INS gene are known to affect the various stages of insulin biosynthesis in the beta cells of the pancreas. However only individual cases of diabetes mellitus (DM) associated with heterozygous mutations in the coding region of the INS gene were reported in Russian Federation. We report a group of patients with a clinical manifestation of DM caused by mutations in both coding and non-coding regions of the INS gene. The patients with a mutation in the intron of the INS gene are reported for the first time in Russian FederationMaterials and methods: 60 patients with an isolated course of neonatal DM (NDM), 52 patients with a manifestation of DM at the age of 7–12 months and the absence of the main autoimmune markers of type 1 DM, 650 patients with the MODY phenotype were included in the study. NGS technology was used for molecular genetic research. Author’s panel of primers (Custom DNA Panel) was used for multiplex PCR and sequencing using Ion Ampliseq™ technology. The author’s panel “­Diabetes Mellitus” included 28 genes (13 candidate genes of MODY and other genes associated with DM).Results: 13 heterozygous mutations were identified in 16 probands and 9 relatives. The majority of mutations were detected in patients with PNDM (18.75%) and in patients with an onset of DM at the age of 7–12 months (9.6%). Mutations in the INS gene were detected in 2 patients (0.3%) in the group with the MODY phenotype. Mutations in the INS gene were not detected in patients with transient NDM (TNDM). Analysis of clinical data in patients with PND and onset of diabetes at the age of 7–12 months did not show significant differences in the course of the disease. The clinical characteristics of the cases of MODY10 and diabetes caused by a mutation in the intron of the INS gene are reported in details.Conclusion: The role of INS gene mutations in NDM, MODY, and DM with an onset at the age of 7–12 months was analyzed in a large group of patients. The clinical characteristics of DM due to a mutation in the intron of the INS gene are reported for the first time in the Russian Federation.

Morphological and molecular data to determine the origin and taxonomic status of Prosopis chilensis var. riojana (Fabaceae, Mimosoideae)

2003 ◽  
Vol 81 (9) ◽  
pp. 905-917 ◽  
Author(s):  
Soledad Vázquez-Garcidueñas ◽  
Ramón A Palacios ◽  
Jenny Segovia-Quiroz ◽  
Juan T Frías-Hernández ◽  
Victor Olalde-Portugal ◽  
...  
Keyword(s):  
Random Amplified Polymorphic Dna ◽  
Taxonomic Status ◽  
Molecular Data ◽  
Genetic Distances ◽  
Group Method ◽  
Sympatric Populations ◽  
Arithmetic Means ◽  
Genetic Characteristics ◽  
Genetic Traits ◽  
Prosopis Chilensis

This study analyzes the morphological and genetic characteristics of three sympatric populations of Prosopis from Argentina. Although morphological and geographical data suggest that Prosopis chilensis var. riojana is an interspecific hybrid of Prosopis chilensis var. chilensis and Prosopis flexuosa var. flexuosa, no correlation was found between morphological traits and genetic distances generated by random amplified polymorphic DNA - polymerase chain reaction (RAPD-PCR). Genetic similarity is greater among P. chilensis var. chilensis and P. flexuosa var. flexuosa than between either of these two taxa with P. chilensis var. riojana. Also, P. chilensis var. riojana has unique genetic markers that are absent from its putative parents. Additionally, dendrograms generated by unweighted pair group method with arithmetic means (UPGMA) and Neighbor-Joining clustering criteria group P. chilensis var. chilensis and P. flexuosa var. flexuosa apart from P. chilensis var. riojana. Possibilities for the lack of congruence between morphology and RAPD markers are discussed. The results obtained are insufficient to conclusively establish the origin of P. chilensis var. riojana; however, the morphological and genetic traits observed suggest this taxon is well differentiated from P. chilensis var. chilensis and P. flexuosa var. flexuosa.Key words: genetic distances, hybridization, morphology, Prosopis, RAPD, rare taxon.

Remarks on some European Aleocharinae, with description of a new Rhopaletes species from Croatia (Coleoptera: Staphylinidae)

2010 ◽  
Vol 53 (1) ◽  
pp. 191-215 ◽  
Author(s):  
László Ádám
Keyword(s):  
New Species ◽  
Taxonomic Status ◽  
Species Group ◽  
Type Material ◽  
Publication Date ◽  
Central European ◽  
European Species ◽  
New Name ◽  
First Time ◽  
A New Species

Remarks on some European Aleocharinae, with description of a new Rhopaletes species from Croatia (Coleoptera: Staphylinidae) Based on an examination of type and non-type material, ten species-group names are synonymised: Atheta mediterranea G. Benick, 1941, Aloconota carpathica Jeannel et Jarrige, 1949 and Atheta carpatensis Tichomirova, 1973 with Aloconota mihoki (Bernhauer, 1913); Amischa jugorum Scheerpeltz, 1956 with Amischa analis (Gravenhorst, 1802); Amischa strupii Scheerpeltz, 1967 with Amischa bifoveolata (Mannerheim, 1830); Atheta tricholomatobia V. B. Semenov, 2002 with Atheta boehmei Linke, 1934; Atheta palatina G. Benick, 1974 and Atheta palatina G. Benick, 1975 with Atheta dilaticornis (Kraatz, 1856); Atheta degenerata G. Benick, 1974 and Atheta degenerata G. Benick, 1975 with Atheta testaceipes (Heer, 1839). A new name, Atheta velebitica nom. nov. is proposed for Atheta serotina Ádám, 2008, a junior primary homonym of Atheta serotina Blackwelder, 1944. A revised key for the Central European species of the Aloconota sulcifrons group is provided. Comments on the separation of the males of Amischa bifoveolata and A. analis are given. A key for the identification of Amischa species occurring in Hungary and its close surroundings is presented. Remarks are presented about the relationships of Alevonota Thomson, 1858 and Enalodroma Thomson, 1859. The taxonomic status of Oxypodera Bernhauer, 1915 and Mycetota Ádám, 1987 is discussed. The specific status of Pella hampei (Kraatz, 1862) is debated. Remarks are presented about the relationships of Alevonota Thomson, 1858, as well as Mycetota Ádám, 1987, Oxypodera Bernhauer, 1915 and Rhopaletes Cameron, 1939. The publication date of several Atheta species described by G. Benick is discussed. Aloconota mihoki, Amischa forcipata, A. filum and Atheta boehmei are reported from Hungary, Croatia and Romania, respectively, for the first time. A new species, Rhopaletes slavoniae sp. n. is described from Croatia.

scholarly journals Maritime trap-jaw ants (Hymenoptera, Formicidae, Ponerinae) of the Indo-Australian region – redescription of Odontomachus malignus Smith and description of a related new species from Singapore, including first descriptions of males

ZooKeys ◽  
2020 ◽  
Vol 915 ◽  
pp. 137-174
Author(s):  
Wendy Y. Wang ◽  
Aiki Yamada ◽  
Seiki Yamane
Keyword(s):  
New Species ◽  
Morphological Characteristics ◽  
Species Group ◽  
The Philippines ◽  
Dna Evidence ◽  
Littoral Habitat ◽  
Morphological Differences ◽  
Worker Caste ◽  
First Time ◽  
Distinct Morphology

The maritime trap-jaw ant Odontomachus malignus Smith, 1859 is thought to be widespread throughout islands in the Indo-Pacific and parts of the Oriental realm. Because of its unique nesting preference for harsh littoral habitat and distinct morphology, O. malignus has usually been assumed to consist of only one species. We, however, describe a new species similar to O. malignus found in the mangroves of Singapore, Southeast Asia – Odontomachus litoralissp. nov. We find strong evidence of both species existing in (near) sympatry, and also distinct morphological differences between O. malignus and the new species. Additional complementary DNA evidence in the form of COI barcodes (313 bp) supporting putative species identification and delimitation is provided. Defining morphological characteristics for the O. malignus species group (nested within the larger O. infandus clade) are given in detail for the first time. The worker and queen castes of the new species are described; a redescription of the worker caste of O. malignus, based on specimens from Singapore and the Philippines in addition to the holotype, is also given. The males of both species are also described for the first time, including male genitalia. A preliminary key to most known species of the O. infandus group based on the worker caste is provided.

scholarly journals New molecular sequences for two genera of marine planarians facilitate determination of their position in the phylogenetic tree, with new records for two species (Platyhelminthes, Tricladida, Maricola)

ZooKeys ◽  
2018 ◽  
Vol 781 ◽  
pp. 1-17 ◽  
Author(s):  
Hee-Min Yang ◽  
Ronald Sluys ◽  
Masaharu Kawakatsu ◽  
Gi-Sik Min
Keyword(s):  
Phylogenetic Tree ◽  
New Records ◽  
Taxonomic Status ◽  
Molecular Data ◽  
Phylogenetic Position ◽  
Molecular Sequences ◽  
Histological Studies ◽  
Anatomical Features ◽  
First Time

For the first time, molecular sequences of the 18S ribosomal DNA were generated for representatives of the genera Obrimoposthia Sluys & Ball, 1989 and Paucumara Sluys, 1989 of the suborder of the marine triclads, or Maricola, by analyzing the species Obrimoposthiawandeli (Hallez, 1906) and Paucumaratrigonocephala (Ijima & Kaburaki, 1916). On the basis of this molecular data the phylogenetic position of these two genera in the phylogenetic tree of the Maricola was determined and compared with their position in the phylogeny based on the analysis of anatomical features. New records for these two species are documented and their taxonomic status is determined on the basis of histological studies.

A new species of Paramesotriton (Caudata: Salamandridae) from Guizhou Province, China

Zootaxa ◽  
2012 ◽  
Vol 3510 (1) ◽  
pp. 41 ◽  
Author(s):  
XIAOMING GU ◽  
RONGRONG CHEN ◽  
YINGZHOU TIAN ◽  
SONG LI ◽  
JINGCHENG RAN
Keyword(s):  
New Species ◽  
Body Size ◽  
Nature Reserve ◽  
Morphological Characteristics ◽  
Molecular Data ◽  
Species Group ◽  
Guizhou Province ◽  
Mitochondrial Data ◽  
A New Species

In this study, we describe a new species of salamander, Paramesotriton maolanensis sp. n., from the MaolanNational Nature Reserve, Libo County, Guizhou Province, China. The new species is placed in the genusParamesotriton based on morphological characteristics and molecular data. It differs from all other members of thegenus in a number of morphological characteristics, especially in its much larger body size, absence of granularwarts from head and body, largely reduced external eyes and peculiar shape of epibranchia in hyoid apparatus. Weexamined the relationships of nuclear POMC haplotypes between and within the new species and six recognizedspecies. POMC variation and published mitochondrial data suggested that the new species’ closest known relativesare P. longliensis, P. zhijinensis and P. caudopunctatus, and it should be placed into the P. caudopunctatus species group or subgenus Allomesotriton.

A new Phoberus MacLeay species (Coleoptera: Scarabaeoidea: Trogidae) from the Maloti-Drakensberg Mountains of southern Africa and the Phoberus caffer species group reviewed

Zootaxa ◽  
2019 ◽  
Vol 4674 (5) ◽  
pp. 551-563
Author(s):  
WERNER P. STRÜMPHER ◽  
CLARKE H. SCHOLTZ
Keyword(s):  
South Africa ◽  
New Species ◽  
Taxonomic Status ◽  
Species Group ◽  
Species Level ◽  
Eastern Cape ◽  
Status Change ◽  
North West ◽  
First Time ◽  
Eastern Highlands

Phoberus ntlenyanae Strümpher, new species is described from Lesotho and South Africa and is placed in the southern African Phoberus caffer group of species. The P. caffer species group is redefined to include the new species. One taxonomic status change is proposed: Phoberus lilianae (Scholtz, 1980) from the Eastern Highlands of Zimbabwe and the eastern escarpment of South Africa is elevated to species level. Phoberus caffer (Harold, 1872) is recorded for the first time from Eastern Cape and North West Province of South Africa. A key is provided for the identification of the members of the group. Photographs illustrate the species, and a map shows their geographical distributions. [Zoobank URL: urn:lsid:zoobank.org:act:0CB1BAD0-F9C2-4303-B746-487BCADF0D07] 

A new predatory leech from Vietnam (Hirudinida : Arhynchobdellida : Salifidae): its phylogenetic position with comments on the classification of the family

2015 ◽  
Vol 29 (5) ◽  
pp. 473 ◽  
Author(s):  
Takafumi Nakano ◽  
Son Truong Nguyen
Keyword(s):  
Phylogenetic Relationships ◽  
Phylogenetic Analyses ◽  
Morphological Characteristics ◽  
Molecular Data ◽  
Phylogenetic Position ◽  
12S Rrna ◽  
The Family ◽  
Morphological And Molecular Data ◽  
First Time

The family Salifidae is a predaceous leech taxon in the suborder Erpobdelliformes. Although Salifidae is widely distributed in the African, Oriental, Indo-Malayan, Sino-Japanese and Australasian regions, the phylogenetic relationships of the family Salifidae have never been tested using molecular data obtained from leeches collected from the family distributional range. A salifid species was collected for the first time in Vietnam, and relevant morphological and molecular data are presented here. Because the Vietnamese salifid species possesses unique morphological characteristics among the known salifid species, this species is herein described as a new species, Salifa motokawai, sp. nov. Phylogenetic analyses based on nuclear 18S rRNA and histone H3, as well as mitochondrial cytochrome c oxidase subunit I, tRNACys, tRNAMet, 12S rRNA, tRNAVal, 16S rRNA, tRNALeu and NADH dehydrogenase subunit 1 markers demonstrate that the Vietnamese salifid species is a close congener with the African Salifa perspicax and the Malagasy Linta be. Furthermore, molecular data revealed non-monophyly of the Asian salifid leeches. According to the observed phylogenetic relationships and morphological characteristics of the Vietnamese Salifa motokawai, sp. nov., the current classification of salifid taxa should be revised.

Remarkable sexual dimorphism, rarity and cryptic species: a revision of the ‘aegrota species group’ of the Neotropical butterfly genus Caeruleuptychia with the description of three new species (Lepidoptera, Nymphalidae, Satyrinae)

2018 ◽  
Vol 49 (2) ◽  
pp. 130-182 ◽  
Author(s):  
Shinichi Nakahara ◽  
Thamara Zacca ◽  
Blanca Huertas ◽  
Andrew F. E. Neild ◽  
Jason P. W. Hall ◽  
...  
Keyword(s):  
New Species ◽  
Sexual Dimorphism ◽  
Cryptic Species ◽  
Molecular Data ◽  
Species Group ◽  
Morphological Data ◽  
Phylogenetic Hypothesis ◽  
Morphological And Molecular Data ◽  
Three New Species ◽  
First Time

The ‘aegrota species group’ of the Neotropical nymphalid genus Caeruleuptychia Forster, 1964, in addition to three other superficially similar, enigmatic species in the genus, are revised. A lectotype is designated for Euptychia aegrota Butler, 1867, E. aetherialis Butler, 1877 stat. rev., E. helios Weymer, 1911 and E. pilata Butler, 1867, and C. aetherialis is resurrected from its synonymy with C. aegrota. Caeruleuptychia helios caelestissima Brévignon, 2010, syn. nov., and Magneuptychia keltoumae Brévignon & Benmesbah, 2012, syn. nov. are both regarded as junior subjective synonyms of C. helios (Weymer, 1911), as a result of the discovery and first illustration of the female of this taxon. The female of C. aegrota is also described and illustrated for the first time, and three new species, C. trembathi Willmott, Nakahara, Hall & Neild, sp. nov., C. scripta Nakahara, Zacca & Huertas, sp. nov., and C. maryzenderae Lamas & Nakahara, sp. nov. are described and named. We analyze morphological and molecular data separately, in addition to combining morphological data with molecular data, to provide the first phylogenetic hypothesis for the taxa treated in this revision.

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