scholarly journals Interspecific differences of antioxidant ability of introduced Chaenomeles species with respect to adaptation to the steppe zone conditions

2018 ◽  
Vol 26 (2) ◽  
pp. 132-138 ◽  
Author(s):  
N. Khromykh ◽  
Y. Lykholat ◽  
L. Shupranova ◽  
A. Kabar ◽  
O. Didur ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Reducing Power ◽  
Steppe Zone ◽  
Biologically Active ◽  
Total Phenolic ◽  
Enzymatic System ◽  
Japanese Species ◽  
Study Results

Plants of the genus Chaenomeles are traditionally used in the countries of South-East Asia, due to their high nutritional and health-promoting properties. However, the successful introduction of species promising for gardening from geographically remote areas requires the study of plant ontogeny under the conditions of new habitat. This is a very substantial problem for the steppe zone, where the continental climate has features of aridity and complicates the process of increasing the diversity of fruit crops by introducing the desired species. The present study aims to assess the effectiveness of the protective enzymatic system of different Chaenomeles genotypes subject to a steppe climate as well as the accumulation of the biologically active compounds with high antioxidant capacity. The study was performed on the basis of the introduced horticultural plants collection in the Botanical Garden of the DNU, and the Chaenomeles fruits, leaves, and the seeds were examined. The highest activity of catalase, benzidine-peroxidase and guaiacol-peroxidase, and the greatest enzymes activation during vegetation were found in leaves of Ch. cathayensis and Ch. speciosa, while the lowest activity was in leaves of both Japanese species. The biggest total phenolic content in the isopropanolic plant extracts, determined by Folin–Ciocalteau assay, was found in leaves of Ch. × superba, Ch. × californica and Ch. cathayensis (44.8, 52.8, and 43.6 mg GAE/g WW); a less high level was found in leaves of Ch. japonica and Ch. japonica var. maulei (43.1 and 40.2 mg GAE/g), while the lowest was in leaves of Ch. speciosa (29.3 mg GAE/g). The total flavonoids content determined using the aluminum chloride method, did not differ by variety or species in the plant leaves, being in the range of 2.6–2.9 mg of RE per g WW (accordingly, in leaves of Ch. japonica var. maulei and Ch. × californica). The high total reducing power determined by potassium ferricyanide assay was found in leaves of both hybridogenic species and Ch. cathayensis (respectively, 11.6, 14.1, and 11.4 AAE/g WW); leaves of both Japanese species had slightly lower values and the lowest was in leaves of Ch. speciosa (7.7 AAE/g). In the Chaenomeles fruits, the total phenolic content was the lowest in Ch. speciosa (17.8 mg GAE/g), average in both Japanese species (28.7 and 27.8 mg GAE/g), and the highest (33.3 mg GAE/g) was in Ch. cathayensis. The flavonoid accumulation was highest in the fruits of Ch. cathayensis and Ch. japonica var. maulei (0.67 and 0.63 mg RE/g), intermediate in both hybridogenic species and Ch. japonica (accordingly, 0.57, 0.42 and 0.38 mg RE/g), and the lowest in Ch. speciosa (0.30 mg RE/g). The total reducing power of Chaenomeles fruit was lower as compared to leaves, and decreased from 11.2 to 5.7 mg AAE/g in the series Ch. cathayensis > Ch. × californica > Ch. japonica > Ch. japonica var. maulei > Ch. × superba > Ch. speciosa. High correlation coefficients between total reducing power and total phenols content in the Chaenomeles leaves and fruits (respectively, r = 0.96 and r = 0.95, P < 0.05) confirm the significant contribution of phenolic compounds to the antioxidant capacity. The study results indicate a high antioxidant capacity of the Chaenomeles species in the conditions of the steppe climate due to the antioxidant enzymes activity and the accumulation of a significant amount of phenolic metabolites in leaves and fruits.

Assessment of Total Phenolic Content and Antioxidant Activity of Ficus carica and Olea europaea L. Leaves Extracts

2019 ◽  
Vol 15 (6) ◽  
pp. 583-587
Author(s):  
Abderrahim Benslama ◽  
Amirouche Deghima ◽  
Nadjat Righi
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Olea Europaea ◽  
Phenolic Content ◽  
Pharmaceutical Preparation ◽  
Reducing Power ◽  
Ficus Carica ◽  
Total Phenolic ◽  
Olea Europaéa

Background: Ficus carica and Olea europaea are two medicinal plants which are widely distributed in Algeria, and used in food and traditional medicine. Objective: The objective of this work is the evaluation of the antioxidant capacity of different extracts of F. carica and O. europaea, for ends of flora valorization in Algeria. Methods: The dried leaves of the concerned plants Ficus carica and Olea europaea were submitted to sequential extraction with solvents of increasing polarity to give hexane, chloroform, ethyl acetate and methanolic extracts. The total phenolic and flavonoids were determined spectrophoto-metrically. The antioxidant activity of extracts was evaluated using 1,1’-Diphenyl-1-Picrylhydrazyl (DPPH) radicalscavenging and ferric reducing power test. Results: The results of the quantitative analysis show that the Methanolic Extract (ME) gives the highest yield with 16.43% and 19.98% for F. carica and O. europaea respectively. The analysis show that the highest amount of phenolic compounds was recorded in the ME of O. europaea (171.40±6.79 µg GAE/ mg E), when the the highest amount of flavonoids was recorded in the CHE of the F. carica (34.06± 0.05 µg QE/ mg E). In addition, the results show that the Ethyle Acetat Extract (EAE) exhibit the highest antiradical activity against DPPH free radical with an EC50=45.21±1.12 and 8.20±0.37 µg/ml for F. carica and O. europaea, respectively. Moreover, the EAE of the two plants present the highest reducing effect compared to other extracts at concentration of 200 µg/ml. Conclusion: The obtained results revealed the presence of a strong correlation between the antioxidant activity of the extract and its total phenolic content. Furthermore, they reported that the EAE have a considerable antioxidant capacity. This can be considered as an alternate natural source of antioxidants used generally as additives in food and pharmaceutical preparation.

scholarly journals Determination of Total Phenolic Content and Antioxidant Activity of Six Ornamental Plants

2019 ◽  
Vol 22 (3) ◽  
pp. 79-84
Author(s):  
Mohamad Rafi ◽  
Tanti Yulianti Raga Pertiwi ◽  
Syaefudin Syaefudin
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ornamental Plants ◽  
Reducing Power ◽  
Total Phenolic ◽  
Dry Powder ◽  
Torenia Fournieri ◽  
Hydrangea Macrophylla

Total phenolic content and antioxidant activity from six Indonesian ornamental plants have been studied. Those plants are Yellow Allamanda (Allamanda cathartica L.), Bigleaf Hydrangea (Hydrangea macrophylla (Thunb.) Ser.), Crystal Anthurium (Anthurium crystallinum Linden & André), Kapok Bush (Aerva sanguinolenta (L.) Blume), Siamese Calypha (Acalypha siamensis Oliv. Ex Gage), and Wishbone Flower/Bluewings (Torenia fournieri Linden ex E. Fourn). Extraction was carried out by maceration using ethanol as the extracting solvent. Total phenolic content was determined by the Folin Ciocalteu method. Antioxidant activity was measured using the 2.2-diphenyl-1-picrylhydrazyl (DPPH), reducing power, and cupric ion reducing antioxidant capacity (CUPRAC) method. Based on the results obtained, Bigleaf Hydrangea has the highest yield and total phenolic content about 15.45% and 13.86 mg gallic acid/g dry powder respectively. Siamese Acalypha leaves have the highest antioxidant activity for all methods used namely DPPH, reducing power, and CUPRAC with a value of 180.45; 202,17; and 589.90 µmol trolox/g dry powder, respectively. This indicates that antioxidant activity is not only derived from phenolic compounds because Siamese Acalypha leaves which have lower total phenolic levels than Bigleaf Hydrangea leaves provide higher antioxidant capacity.

EXTRACTION OPTIMIZATION AND ANTIOXIDANT ACTIVITY OF PHENOLIC COMPOUNDS FROM AVOCADO PEEL

2019 ◽  
pp. 49-59
Author(s):  
Nu Linh Giang Ton ◽  
Thi Hoai Nguyen ◽  
Quoc Hung Vo
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Antioxidant Capacity ◽  
Response Surface ◽  
Total Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Quadratic Model ◽  
Total Phenolic ◽  
Total Antioxidant

Avocado peel has been considered as a potential source of natural antioxidants in which phenolics are among the most important compounds. Therefore, this study aims to optimize the extraction process of phenolics using response surface methodology and evaluate the corresponding antioxidant activity. From the quadratic model, the optimal condition was determined including the ethanol concentration 54.55% (v/v), the solvent/solute ratio 71.82/1 (mL/g), temperature 53.03 oC and extraction time 99.09 min. The total phenolic content and the total antioxidant capacity at this condition with minor modifications were 26,74 ± 0,04 (mg GAE/g DW) and 188.06 ± 1.41 (mg AAE/g DW), respectively. The significant correlation between total phenolic content and total antioxidant capacity was also confirmed. Key words: response surface methodology, central composite rotatable design, total phenolic content, total antioxidant capacity, avocado peel

scholarly journals Optimization of Natural Antioxidants Extraction from Pineapple Peel and Their Stabilization by Spray Drying

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1255
Author(s):  
Sofia C. Lourenço ◽  
Débora A. Campos ◽  
Ricardo Gómez-García ◽  
Manuela Pintado ◽  
M. Conceição Oliveira ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Spray Drying ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Food Products ◽  
Total Phenolic ◽  
Dry Extract ◽  
Pineapple Peel ◽  
Dpph Method

Pineapple peel still contains an important amount of phenolic compounds and vitamins with valuable antioxidant activity. In this way, the aim of this study was the recovery of the bioactive compounds from pineapple peel using environmentally friendly and low-cost techniques, envisaging their application in food products. From the solid-liquid extraction conditions tested, the one delivering an extract with higher total phenolic content and antioxidant capacity was a single extraction step with a solvent-pineapple peel ratio of 1:1 (w/w) for 25 min at ambient temperature, using ethanol-water (80–20%) as a solvent. The resulting extract revealed a total phenolic content value of 11.10 ± 0.01 mg gallic acid equivalent (GAE)/g dry extract, antioxidant activity of 91.79 ± 1.98 µmol Trolox/g dry extract by the DPPH method, and 174.50 ± 9.98 µmol Trolox/g dry extract by the FRAP method. The antioxidant rich extract was subjected to stabilization by the spray drying process at 150 °C of inlet air temperature using maltodextrin (5% w/w) as an encapsulating agent. The results showed that the antioxidant capacity of the encapsulated compounds was maintained after encapsulation. The loaded microparticles obtained, which consist of a bioactive powder, present a great potential to be incorporated in food products or to produce bioactive packaging systems.

scholarly journals New insights of phenolic compounds from optimized fruit extract of Ficus auriculata

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
M. Shahinuzzaman ◽  
Parul Akhtar ◽  
N. Amin ◽  
Yunus Ahmed ◽  
Farah Hannan Anuar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Fruit Extract ◽  
Optimum Extraction

AbstractIn this study, the extraction conditions extracted maximize amounts of phenolic and bioactive compounds from the fruit extract of Ficus auriculata by using optimized response surface methodology. The antioxidant capacity was evaluated through the assay of radical scavenging ability on DPPH and ABTS as well as reducing power assays on total phenolic content (TPC). For the extraction purpose, the ultrasonic assisted extraction technique was employed. A second-order polynomial model satisfactorily fitted to the experimental findings concerning antioxidant activity (R2 = 0.968, P < 0.0001) and total phenolic content (R2 = 0.961, P < 0.0001), indicating a significant correlation between the experimental and expected value. The highest DPPH radical scavenging activity was achieved 85.20 ± 0.96% at the optimum extraction parameters of 52.5% ethanol (v/v), 40.0 °C temperature, and 22 min extraction time. Alternatively, the highest yield of total phenolic content was found 31.65 ± 0.94 mg GAE/g DF at the optimum extraction conditions. From the LC–ESI–MS profiling of the optimized extract, 18 bioactive compounds were tentatively identified, which may regulate the antioxidant activity of fruits of F. auriculata.

scholarly journals Antioxidant Rich Extracts of Terminalia ferdinandiana Inhibit the Growth of Foodborne Bacteria

Foods ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 281 ◽  
Author(s):  
Saleha Akter ◽  
Michael E. Netzel ◽  
Ujang Tinggi ◽  
Simone A. Osborne ◽  
Mary T. Fletcher ◽  
...  
Keyword(s):  
Free Radical ◽  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Food Preservation ◽  
Total Phenolic ◽  
Foodborne Bacteria ◽  
Terminalia Ferdinandiana

Terminalia ferdinandiana (Kakadu plum) is a native Australian plant containing phytochemicals with antioxidant capacity. In the search for alternatives to synthetic preservatives, antioxidants from plants and herbs are increasingly being investigated for the preservation of food. In this study, extracts were prepared from Terminalia ferdinandiana fruit, leaves, seedcoats, and bark using different solvents. Hydrolysable and condensed tannin contents in the extracts were determined, as well as antioxidant capacity, by measuring the total phenolic content (TPC) and free radical scavenging activity using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. Total phenolic content was higher in the fruits and barks with methanol extracts, containing the highest TPC, hydrolysable tannins, and DPPH-free radical scavenging capacity (12.2 ± 2.8 g/100 g dry weight (DW), 55 ± 2 mg/100 g DW, and 93% respectively). Saponins and condensed tannins were highest in bark extracts (7.0 ± 0.2 and 6.5 ± 0.7 g/100 g DW). The antimicrobial activity of extracts from fruit and leaves showed larger zones of inhibition, compared to seedcoats and barks, against the foodborne bacteria Listeria monocytogenes, Bacillus cereus, Methicillin resistant Staphylococcus aureus, and clinical isolates of Pseudomonas aeruginosa. The minimum inhibitory concentration and minimum bactericidal concentration in response to the different extracts ranged from 1.0 to 3.0 mg/mL. Scanning electron microscopy images of the treated bacteria showed morphological changes, leading to cell death. These results suggest that antioxidant rich extracts of Terminalia ferdinandiana fruits and leaves have potential applications as natural antimicrobials in food preservation.

scholarly journals Polyphenols in Coreopsis tinctoria Nutt. fruits and the plant extracts antioxidant capacity evaluation

2014 ◽  
Vol 12 (8) ◽  
pp. 858-867 ◽  
Author(s):  
Christina Zălaru ◽  
Claudia Crişan ◽  
Ioan Călinescu ◽  
Zenovia Moldovan ◽  
Isabela Ţârcomnicu ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Plant Extracts ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Optimum Operating ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Ultrasound Assisted Extraction ◽  
Coreopsis Tinctoria

AbstractThe aim of this research was to investigate the chemical composition of Coreopsis tinctoria Nutt. fruits extract, to highlight the potential of ultrasound assisted extraction in the fast preparation of extracts rich in polyphenols using different solvents (55%, 78% and 96% hydrous ethanol) and to evaluate the antioxidant potential of formulated extracts. LC-MS/MS was used to characterize the ethanolic extract from Coreopsis tinctoria Nutt. dried fruits. The extract contains different flavonoids (marein, flavanomarein, quercetagetin-7-O-glucoside, okanin aurone, leptosidin, luteolin, apigenin) and phenolic acids (chlorogenic acid, caffeic acid). Several parameters that could affect extraction efficiency were evaluated. Finally, this study focused on determination of plant extracts total phenolic content and their antioxidant capacity. The experimental results allowed the selection of the optimum operating parameters leading to the highest total polyphenolic content, expressed as gallic acid equivalents, and avoiding the degradation of phenolic compounds (ethanol 55%; extraction temperature 323.15 K, extraction time 30 min, liquid/solid ratio 20/1). A good relationship between total phenolic content and antioxidant capacity was obtained.

Sign in / Sign up

Export Citation Format

Share Document