scholarly journals A Validated High Performance Thin Layer Chromatographic Method for Simultaneous Estimation of Berberine Chloride and Guggulsterone Z in Herbal Formulation

2019 ◽  
Vol 7 (1) ◽  
pp. 7-13
Author(s):  
Vijaykumar K. Parmar ◽  
Deepika Mohanta ◽  
Harsh Shah
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Simultaneous Estimation ◽  
Berberine Chloride ◽  
Herbal Formulation ◽  
Ich Guidelines ◽  
System Suitability ◽  
Layer Chromatography ◽  
Hptlc Method

A simple, precise, and robust high-performance thin layer chromatography (HPTLC) method was developed and validated for the determination of berberine chloride and guggulsterone Z in herbal formulation. Chromatographic separation was achieved on aluminium plates precoated with silica gel G60F254 as the stationary phase and toluene-acetonitrile-formic acid (5:3:0.5 v/v/v) as the mobile phase. Densitometric evaluation was carried out at 264 nm. The present method was validated according to ICH guidelines. The Rf value of berberine chloride and guggulsterone Z was found to be 0.40 ± 0.02 and 0.68 ± 0.02, respectively. The response in terms of peak area was found to be linear over the concentration range of 100-500 ng/spot for berberine chloride and 200-1000 ng/spot for guggulsterone Z with regression coefficient value greater than 0.995 for both the phytoconstituents. The method was validated by determining its accuracy, precision, robustness, specificity and system suitability. The method was found to be accurate, precise and robust to carry out the simultaneous estimation of berberine chloride and guggulsterone Z. The developed method was successfully applied for the simultaneous estimation of berberine chloride and guggulsterone Z in herbal formulation.

scholarly journals IDENTIFICATION, QUANTIFICATION AND VALIDATION OF STIGMASTEROL FROM ALPINIA CALCARATA USING HIGH-PERFORMANCE THIN LAYER CHROMATOGRAPHY METHOD

2017 ◽  
Vol 9 (9) ◽  
pp. 126
Author(s):  
Pratheema Philomindoss
Keyword(s):  
Linear Regression ◽  
Thin Layer ◽  
Linear Relationship ◽  
High Performance ◽  
Retention Factor ◽  
Chromatography Method ◽  
Ich Guidelines ◽  
Layer Chromatography ◽  
Hptlc Method

Objective: The present study is designed to develop a new simple, precise, rapid and selective high‐performance thin‐layer chromatographic (HPTLC) method for the determination of stigmasterol in methanolic rhizomes extract of Alpinia calcarata.Methods: As per International Conference on Harmonization (ICH) guidelines we have applied different concentrations of stigmasterol as standard on HPTLC plates for the quantification of stigmasterol from the Alpinia calcarata rhizomes. The concentration of standard stigmasterol is 1 mg/ml.Results: The retention factor of stigmasterol was 0.58. Linearity was obtained in the range of 50 ng‐250 ng for stigmasterol. The developed and validated HPTLC method was employed for stigmasterol in methanolic rhizomes extract of Alpinia calcarata for standardization of the content of the marker. The linear regression data for the calibration plots showed a good linear relationship with r=0.99977 for stigmasterol, respectively Satisfactory recoveries of 99.77 % were obtained for stigmasterol.Conclusion: The results obtained in validation assays indicate the accuracy and reliability of the developed HPTLC method for the quantification of stigmasterol in methanolic rhizomes extract of Alpinia calcarata

scholarly journals DETERMINATION OF 10-GINGEROL IN INDIAN GINGER BY VALIDATED HPTLC METHOD OF SAMPLES COLLECTED ACROSS SUBCONTINENT OF INDIA

2016 ◽  
Vol 8 (12) ◽  
pp. 190
Author(s):  
Kamran Ashraf ◽  
Syed Adnan Ali Shah ◽  
Mohd Mujeeb
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Limit Of Detection ◽  
Limit Of Quantification ◽  
Chromatography Method ◽  
Aluminum Plates ◽  
Layer Chromatography ◽  
Hptlc Method

<p><strong>Objective: </strong>A simple, sensitive, precise, and accurate stability indicating HPTLC (high-performance thin-layer chromatography) method for analysis of 10-gingerol in ginger has been developed and validated as perICH guidelines.</p><p><strong>Methods: </strong>The separation was achieved on TLC (thin layer chromatography) aluminum plates pre-coated with silica gel 60F<sub>254</sub> using n-hexane: ethyl acetate 55:45 (%, v/v) as a mobile phase. Densitometric analysis was performed at 569 nm.</p><p><strong>Results: </strong>This system was found to have a compact spot of 10-gingerol at <em>R</em><sub>F</sub> value of 0.57±0.03. For the proposed procedure, linearity (<em>r</em><sup>2</sup> = 0.998±0.02), limit of detection (18ng/spot), limit of quantification (42 ng/spot), recovery (ranging from 98.35%–100.68%), were found to be satisfactory.</p><p><strong>Conclusion: </strong>Statistical analysis reveals that the content of 10-gingerol in different geographical region varied significantly. The highest and lowest concentration of 10-gingerol in ginger was found to be present in a sample of Patna, Lucknow and Surat respectively which inferred that the variety of ginger found in Patna, Lucknow are much superior to other regions of India.</p>

DEVELOPMENT OF HPTLC METHOD FOR SIMULTANEOUS ESTIMATION OF CIPROFLOXACIN AND ORNIDAZOLE IN COMBINED DOSAGE FORM

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (05) ◽  
pp. 36-43
Author(s):  
N. R Dighade ◽  
◽  
M. D Shende ◽  
A. V Kasture
Keyword(s):  
Quality Control ◽  
High Resolution ◽  
Thin Layer ◽  
Mobile Phase ◽  
High Performance ◽  
Dosage Form ◽  
Simultaneous Estimation ◽  
Ich Guidelines ◽  
Hptlc Method ◽  
Routine Quality Control

A simple and accurate high performance thin layer chromatographic (HPLTC) method has been developed and validated as per ICH guidelines for estimations of Ciprofloxacin (CP) and Ornidazole (ORN) in combined dosage form. The mobile phase was acetonitrile: toluene: water and triethylamine (5.5:1.8:1.5:1.6 V/V) was found to be best which gave high resolution with Rf 0.16 and 0.84 for ciprofloxacin and ornidazole respectively. The linearity of ciprofloxacin and ornidazole was found to be in the range of 0.4 to 0.8 µg/mL and 0.4 to 0.8 µg/mL, respectively. The coefficient of correlation (r2 ) was found to be greater than 0.989 for both the components by this method. The tablet analyses result (n = 5) were found to be > 100.84 % by HPTLC for both the components. The proposed method was found to be simple, accurate and suitable for routine quality control of marketed formulations containing these drugs.

Radial "high-performance" thin-layer chromatography used to assess fetal lung maturity

1990 ◽  
Vol 36 (5) ◽  
pp. 728-731 ◽  
Author(s):  
J R Mackenzie ◽  
M Truesdale
Keyword(s):  
Thin Layer ◽  
Amniotic Fluid ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Fetal Lung ◽  
Additional Method ◽  
Layer Chromatography ◽  
Hptlc Method ◽  
Lung Maturity

Abstract A radial "high-performance" thin-layer chromatographic (HPTLC) method is described by which the percentages and ratios of phosphatidylinositol, sphingomyelin, lecithin, phosphatidylethanolamine, phosphatidylglycerol, and dimethyl phosphatidylethanolamine may be determined simultaneously. An additional method for radial HPTLC determination of saturated phosphatidylcholine is described. We report results of application of these methods to greater than 2000 specimens of amniotic fluid from both diabetic and nondiabetic cases.

scholarly journals Development of High-performance Thin-layer Chromatography (HPTLC) Validated Method for Simultaneous Quantification of Eucalyptol and α-Pinene in Lamiaceae Plants

2019 ◽  
pp. 1-11 ◽  
Author(s):  
Ahmed Ibrahim Foudah ◽  
Prawez Alam ◽  
Aftab Alam ◽  
Mohammed Ayman Salkini ◽  
Mohammed Hamed Alqarni ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Chromatographic Technique ◽  
Microbial Infections ◽  
Traditional Preparation ◽  
Layer Chromatography ◽  
Hptlc Method ◽  
Ich Guideline

Aim and Objective: Several plants from Lamiaceae family are used in the Saudi Arabia as a condiment and food preparation, and are generally used in the traditional preparation to treat various diseases, including anti-inflammatory, antioxidant, and microbial infections. Some of Lamiaceae species such as Mentha longifolia, Rosemarinus officinalis and Salvia officinalis having pharmacological active compounds such as α-pinene and eucalyptol. The aim of present study was to develop an accurate and precise chromatographic technique for quantification of α-pinene and eucalyptol in the Lamiaceae plants.  Methods: The high-performance thin layer chromatography (HPTLC) method was developed as per International Conference on Harmonization (ICH) guideline. Results: Simultaneous determination of α-pinene and eucalyptol was achieved by developing a densitometric analysis of high-performance thin layer chromatography (HPTLC). Silica gel 60 F254 glass-backed plates (E-Merck, Germany, 0.2 mm layers) as stationary phase and mixture n-hexane: ethyl acetate 8 : 2 (%, v/v) as mobile phase were used to produce a sharp, symmetrical and well-resolved peak at an Rf value of 0.19 ± 0.02 and 0.52 ± 0.04 for α-pinene and eucalyptol, respectively. Linearly range for α-pinene was 100–700 ng/spot (r2 = 0.9988), whereas that for eucalyptol was 1000–7000 ng/spot (r2 = 0.9987). Conclusion: The developed method was found to be a simple, accurate, and precise, and it may be used to simultaneously analyses of many medicinal plants samples containing α-pinene and eucalyptol.

scholarly journals A RAPID VALIDATED UNI-DIMENSIONAL DOUBLE DEVELOPMENT HPTLC-DENSITOMETRY METHOD FOR SIMULTANEOUS ESTIMATION OF METFORMIN HYDROCHLORIDE, GLICLAZIDE AND PIOGLITAZONE HYDROCHLORIDE

2019 ◽  
pp. 74-80
Author(s):  
Rajesh Varade ◽  
Harsha Mishra
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Metformin Hydrochloride ◽  
Simultaneous Estimation ◽  
Good Resolution ◽  
Diabetic Medicine ◽  
Ich Guidelines ◽  
Validation Parameters ◽  
Layer Chromatography

Objective: To develop and validate a uni-dimensional double development high-performance thin layer chromatography (UDDD-HPTLC) for estimation of anti-diabetic medicine compromising of metformin (MET) gliclazide (GLZ) and pioglitazone hydrochloride (PIO). Methods: The chromatographic separation of these drugs was carried out on precoated TLC plates silica gel 60F254by two mobile phases consisting of Ammonium Sulphate: Methanol: Acetonitrile: Water (4:3:2:1) for MET and PIO and Toluene: Ethyl Acetate: Formic Acid (6:4:0.5) for GLZ respectively for ideal separation and good resolution. The densitometric detection and quantification were carried out at 237 nm for MET and 200 nm for GLZ and PIO. The validation parameters were strictly followed as per the ICH guidelines. Results: The linearity range was obtained at 3000-8000ng/spot, 360-960 ng/spot, 90-240 ng/spot for MET, GLZ and PIO with r2value>0.999. The other parameters such as precision, reproducibility, robustness were efficiently obtained within the limits. The proposed method was successfully applied for simultaneous determination of MET, GLZ and PIO in the commercial formulation. Conclusion: In simultaneous estimation, the different polarity of drugs makes it more cumbersome to develop and validate any chromatographic method. In the present study, a uni-dimensional double development high-performance thin layer chromatography (UDDD-HPTLC) for estimation of these drugs have been developed and validated to resolve the estimation problem. It is an effortless and speedy method which was developed and validated using ICH guidelines. The developed and validated method using ICH guidelines is effortless and speedy technique.

scholarly journals Ecofriendly densitometric RP-HPTLC method for determination of rivaroxaban in nanoparticle formulations using green solvents

RSC Advances ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 2133-2140 ◽  
Author(s):  
Prawez Alam ◽  
Essam Ezzeldin ◽  
Muzaffar Iqbal ◽  
Md. Khalid Anwer ◽  
Gamal A. E. Mostafa ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Reversed Phase ◽  
Literature Survey ◽  
Green Solvents ◽  
Layer Chromatography ◽  
Hptlc Method

A literature survey revealed no suitable “reversed phase-high performance thin layer chromatography (RP-HPTLC)” method for the analysis of rivaroxaban in nanoparticle (NP) formulations.

scholarly journals Determination of Active Ingredient in Synthetic Pyrethroid Formulations by High-Performance Thin-Layer Chromatography/Densitometry

2002 ◽  
Vol 85 (6) ◽  
pp. 1420-1424 ◽  
Author(s):  
Krishan K Sharma
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Active Ingredient ◽  
High Performance ◽  
Synthetic Pyrethroid ◽  
Gas Liquid Chromatography ◽  
Layer Chromatography ◽  
Hptlc Method ◽  
Ec Formulations

Abstract A new, simple, precise, and rapid high-performance thin-layer chromatography (HPTLC) method was developed for the analysis of commercial emulsifiable concentrate (EC) formulations of cypermethrin, α-cypermethrin, and λ-cyhalothrin. A known amount of reference grade synthetic pyrethroid and its EC formulation was subjected to TLC, and the amount of pesticide present in the active ingredient spot was estimated by densitometry in a single beam, single wavelength reflectance mode. Calibration curves of the synthetic pyrethroid was linear in the range of 8–24 μg, and the correlation coefficient for the calibration equation ranged between 0.97 and 0.99. Recoveries from laboratory-prepared test samples of the EC formulation were in the range of 95–99%. Pesticide formulations were further analyzed by gas liquid chromatography using flame ionization detector, and results were comparable. The proposed HPTLC method has application for quality control and determination of the shelf life of EC formulations.

scholarly journals A High-Performance Thin Layer Chromatography (HPTLC) Method for Simultaneous Determination of Diphenhydramine Hydrochloride and Naproxen Sodium in Tablets

2015 ◽  
Vol 10 ◽  
pp. ACI.S31506 ◽  
Author(s):  
R.P. Bhole ◽  
S.S. Shinde ◽  
S.S. Chitlange ◽  
S.B. Wankhede
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Simultaneous Determination ◽  
High Performance ◽  
Naproxen Sodium ◽  
Diphenhydramine Hydrochloride ◽  
Limit Of Quantitation ◽  
Layer Chromatography ◽  
Hptlc Method

A rapid and simple high-performance thin layer chromatography (HPTLC) method with densitometry at 230 nm was developed and validated for simultaneous determination of diphenhydramine hydrochloride (DPH) and naproxen sodium (NPS) from pharmaceutical preparation. The separation was carried out on aluminum plates precoated with silica gel 60 F254 using mobile phase toluene:methanol:glacial acetic acid (7.5:1:0.2, v/v/v). The linearity range lies between 200 and 1200 ng/band for DPH and 1760 and 10,560 ng/band for NPS with correlation coefficients of 0.994 and 0.995, respectively. The Rf value for DPH is 0.20 ± 0.05 and for NPS is 0.61 ± 0.06. % Recoveries of DPH and NPS was in the range of 99.70%-99.95% and 99.63%-99.95%, respectively. Limit of detection value for DPH was 13.21 ng/band and for NPS was 8.03 ng/band. Limit of quantitation value for DPH was 40.06 ng/band and for NPS was 24.34 ng/band. The developed method was validated as per ICH guidelines. In stability testing, DPH was found unstable to acid and alkaline hydrolysis, and DPH and NPS were found unstable to oxidation, whereas both the drugs were stable to neutral and photodegradation. The proposed method was successfully applied for the routine quantitative analysis of dosage form containing DPH and NPS.

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