scholarly journals PRODUCTION, PURIFICATION AND CHARACTERIZATION OF AN ANTIBACTERIAL COMPOUND FROM STREPTOMYCES GRISEUS TBG19NRA1 ISOLATED FROM THE FOREST SOILS OF THE WESTERN GHATS OF KERALA, INDIA

2021 ◽  
Vol 10 ◽  
Author(s):  
Shiburaj Sugathan
Keyword(s):  
Forest Soils ◽  
Western Ghats ◽  
Streptomyces Griseus ◽  
Purification And Characterization ◽  
Antibacterial Compound ◽  
The Western Ghats

Purification and characterization of a 49-kDa chitinase from Streptomyces griseus HUT 6037

2000 ◽  
Vol 89 (1) ◽  
pp. 27-32 ◽  
Author(s):  
Toshiaki Tanabe ◽  
Tomokazu Kawase ◽  
Takeshi Watanabe ◽  
Yasushi Uchida ◽  
Masaru Mitsutomi
Keyword(s):  
Streptomyces Griseus ◽  
Purification And Characterization

scholarly journals Can proteomics of snake venoms help drug discovery? A study on the venom of spectacled cobra (Naja naja) from the Western Ghats

2021 ◽  
Author(s):  
Muralidharan Vanuopadath ◽  
Dileepkumar Raveendran ◽  
Bipin Gopalakrishnan Nair ◽  
Sudarslal Sadasivan Nair
Keyword(s):  
Glutathione Peroxidase ◽  
Western Ghats ◽  
De Novo ◽  
Cross Reactivity ◽  
Cobra Venom ◽  
Venom Protein ◽  
Naja Naja ◽  
First Time ◽  
The Western Ghats

AbstractVenom proteome profiling is important to understand the toxicology and treatment of persons poisoned by animal venoms. An in depth understanding of the pharmacological mechanisms induced by venom toxins could help in the discovery of novel drug molecules. In the current study, we aimed to delineate the venom toxins of Indian cobra (Naja naja) from the Western Ghats of India through SDS-PAGE and reversed-phase HPLC followed by Q-TOF LC-MS/MS analysis, incorporating PEAKS and Novor assisted de novo sequencing methodologies. A total of 143 proteins distributed across 17 different enzymatic and non-enzymatic venom protein families were identified. The de novo analysis exclusively yielded 59 peptides representing 28 venom protein families. Among these, glutathione peroxidase and endonuclease were reported for the first time in Indian cobra venom. Immunological cross-reactivity of cobra venom assessed using Indian polyvalent antivenoms suggested that VINS showed better EC50 (2.48 µg/mL) values than that of PSAV (6.04 µg/mL) and Virchow (6.03 µg/mL) antivenoms. Also, immunoaffinity chromatography performed using VINS antivenom indicated that it failed to detect few low molecular mass proteins (<10 kDa) that include three-finger toxins, phospholipase A2s and kunitz-type serine protease inhibitors. Taken together, the present study enabled a large-scale characterization of the venom proteome of Naja naja that offers valuable insights on the possible pharmacological mechanisms and future therapeutic potential of hitherto unexplored snake venom constituents.SignificanceThe present work describes the venom proteome characterization of Naja naja collected from the Western Ghats region in India, incorporating conventional proteomics approaches as well as de novo sequencing methods. Interestingly, we were able to determine proteins belong to glutathione peroxidase and endonuclease family, which was not reported in any of the previous studies on Naja naja venom. Notably, our study has reported the highest number of proteins from cobra venom so far. Also, the current study highlights the importance of developing region-specific antivenoms for improving the specificity and cross-neutralization potential of antivenoms.HighlightsProteomics of cobra venom resulted in the identification of 143 proteins.De novo approaches exclusively yielded 59 peptides representing 28 proteins.Glutathione peroxidase and endonuclease were identified for the first time in Indian cobra venom.Indian polyvalent antivenoms showed varying cross-reactivity towards cobra venom.VINS antivenom failed to detect few low molecular mass proteins (< 10 kDa).

Synopsis and lectotypification of Distimake rhyncorhiza (Dalzell) Simões & Staples (Convolvulaceae): a little known species from the Western Ghats (India)

Phytotaxa ◽  
2018 ◽  
Vol 336 (3) ◽  
pp. 293
Author(s):  
ANA RITA SIMÕES ◽  
SUSHANT MORE
Keyword(s):  
Western Ghats ◽  
Conservation Status ◽  
Distinct Species ◽  
Morphological Evidence ◽  
Morphological Description ◽  
Western India ◽  
Full Characterization ◽  
First Time ◽  
The Western Ghats

We present a synopsis of a rare and little-known species of Distimake from Western India: a complete morphological description based on recent collections is provided, and nomenclature and typification issues are resolved. The fruit and seeds are described for the first time. With new morphological evidence and a full characterization of D. rhyncorhiza, the present study corroborates its placement in Distimake and supports its identity as a distinct species. The conservation status is assessed as Endangered but its distribution range is not yet fully documented and this assessment might be reviewed if more collections are made.

Purification and characterization of recombinant Streptomyces griseus aminopeptidase

2003 ◽  
Vol 30 (1) ◽  
pp. 62-68 ◽  
Author(s):  
Sally Xueying Ni ◽  
Douglas Cossar ◽  
Adrian Man ◽  
Karen Norek ◽  
David Miller ◽  
...  
Keyword(s):  
Streptomyces Griseus ◽  
Purification And Characterization

scholarly journals CHARACTERIZATION OF THE IRREGULARITY OF A TERRAIN USING FRACTAL DIMENSION OF LAKES' BOUNDARIES

Fractals ◽  
2015 ◽  
Vol 23 (02) ◽  
pp. 1550002 ◽  
Author(s):  
NAKUL N. KARLE ◽  
KIRAN M. KOLWANKAR
Keyword(s):  
Fractal Dimension ◽  
Western Ghats ◽  
Similar Character ◽  
Broad Variation ◽  
Self Similar ◽  
Fractal Character ◽  
The Western Ghats ◽  
Natural Water Body ◽  
Shed Light

Even though many objects and phenomena of importance in geophysics have been shown to have fractal character, there are still many of them which show self-similar character and yet to be studied. The objective of the present work is to demonstrate that the fractal dimension of the boundary of a natural water body can be used to shed light on irregularity as well as other properties of a region. Owing to easy availability of satellite images and image processing softwares, this turns out to be a handy tool. In this study, we have analyzed several lakes in India mostly around the Western Ghats region. We find that the fractal dimension of their boundaries for the length scales between around 40 m to 2 km, in general, has broad variation from 1.2 to 1.6. But when they are grouped into three categories, viz., lakes along the ridge of Western Ghats, lakes in the planes and lakes in the mountain region, we find the first two groups to have a narrower distribution of dimensions.

Sign in / Sign up

Export Citation Format

Share Document