scholarly journals ISOLATION OF LACTIC ACID BACTERIA FROM TRADITIONAL MOROCCAN PRODUCTS AND EVALUATION OF THEIR ANTIFUNGAL ACTIVITY ON GROWTH AND OCHRATOXIN A PRODUCTION BY ASPERGILLUS CARBONARIUS AND A. NIGER

2007 ◽  
Vol 70 (9) ◽  
pp. 2155-2160 ◽  
Author(s):  
VINCENZO DEL PRETE ◽  
HECTOR RODRIGUEZ ◽  
ALFONSO V. CARRASCOSA ◽  
BLANCA de las RIVAS ◽  
EMILIA GARCIA-MORUNO ◽  
...  

A study was carried out to determine the in vitro interaction between ochratoxin A (OTA) and wine lactic acid bacteria (LAB). Fifteen strains belonging to five relevant oenological LAB species were grown in liquid synthetic culture medium containing OTA. The portion of OTA removed during the bacterial growth was 8 to 28%. The OTA removed from the supernatants was partially recovered (31 to 57%) from the bacterial pellet. Cell-free extracts of three representative strains were produced by disrupting cells in a French pressure cell. The ability of crude cell-free extracts to degrade OTA was studied. OTA was not degraded by cell-free extracts of wine LAB strains, and no degradation products of OTA were detected in the high-performance liquid chromatograms of the methanol extract of the bacterial pellet. On the basis of these results, we conclude that OTA removal by wine LAB is a cell-binding phenomenon. The chemistry and the molecular basis of OTA binding to wine LAB remains unknown.


2018 ◽  
Vol 9 ◽  
Author(s):  
Marcia Leyva Salas ◽  
Anne Thierry ◽  
Mathilde Lemaître ◽  
Gilles Garric ◽  
Marielle Harel-Oger ◽  
...  

Toxins ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 700 ◽  
Author(s):  
Randa Zeidan ◽  
Zahoor Ul-Hassan ◽  
Roda Al-Thani ◽  
Quirico Migheli ◽  
Samir Jaoua

Mycotoxins are secondary metabolites produced by certain filamentous fungi, causing human and animal health issues upon the ingestion of contaminated food and feed. Among the safest approaches to the control of mycotoxigenic fungi and mycotoxin detoxification is the application of microbial biocontrol agents. Burkholderia cepacia is known for producing metabolites active against a broad number of pathogenic fungi. In this study, the antifungal potential of a Qatari strain of Burkholderia cepacia (QBC03) was explored. QBC03 exhibited antifungal activity against a wide range of mycotoxigenic, as well as phytopathogenic, fungal genera and species. The QBC03 culture supernatant significantly inhibited the growth of Aspergillus carbonarius, Fusarium culmorum and Penicillium verrucosum in PDA medium, as well as A. carbonarius and P. verrucosum biomass in PDB medium. The QBC03 culture supernatant was found to dramatically reduce the synthesis of ochratoxin A (OTA) by A. carbonarius, in addition to inducing mycelia malformation. The antifungal activity of QBC03’s culture extract was retained following thermal treatment at 100 °C for 30 min. The findings of the present study advocate that QBC03 is a suitable biocontrol agent against toxigenic fungi, due to the inhibitory activity of its thermostable metabolites.


2014 ◽  
Vol 25 (3) ◽  
pp. 245-259 ◽  
Author(s):  
Jean-Justin Essia Ngang ◽  
Germaine Yadang ◽  
Sylvain L. Sado Kamdem ◽  
Christiant Pascal Kouebou ◽  
Sandrine A. Youte Fanche ◽  
...  

2014 ◽  
Vol 76 ◽  
pp. 52-59 ◽  
Author(s):  
Nesrine Belkacem-Hanfi ◽  
Imene Fhoula ◽  
Nabil Semmar ◽  
Amel Guesmi ◽  
Isabelle Perraud-Gaime ◽  
...  

Food Control ◽  
2014 ◽  
Vol 46 ◽  
pp. 91-97 ◽  
Author(s):  
Elsie Y.L. Cheong ◽  
Amrita Sandhu ◽  
Jayaram Jayabalan ◽  
Thu Thi Kieu Le ◽  
Nguyen Thi Nhiep ◽  
...  

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