scholarly journals MICROARRAY-BASED ANALYSIS OF STAPHYLOCOCCUS AUREUS ISOLATES FROM NON-CLINICAL SOURCE

2021 ◽  
Vol 10 (5) ◽  
Author(s):  
Asabe Halimat Momoh-Zekeri
Keyword(s):  
Staphylococcus Aureus ◽  
Clinical Source

scholarly journals Antimicrobial Activity of the Circular Bacteriocin AS-48 against Clinical Multidrug-Resistant Staphylococcus aureus

Antibiotics ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 925
Author(s):  
Cristina Velázquez-Suárez ◽  
Rubén Cebrián ◽  
Carmen Gasca-Capote ◽  
Antonio Sorlózano-Puerto ◽  
José Gutiérrez-Fernández ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Multidrug Resistant ◽  
Resistance Profile ◽  
Risk Of Death ◽  
Antimicrobial Resistance Profile ◽  
The Matrix ◽  
Division Process ◽  
Important Challenge ◽  
Clinical Source

The treatment and hospital-spread-control of methicillin-resistant Staphylococcus aureus (MRSA) is an important challenge since these bacteria are involved in a considerable number of nosocomial infections that are difficult to treat and produce prolonged hospitalization, thus also increasing the risk of death. In fact, MRSA strains are frequently resistant to all β-lactam antibiotics, and co-resistances with other drugs such as macrolides, aminoglycosides, and lincosamides are usually reported, limiting the therapeutical options. To this must be added that the ability of these bacteria to form biofilms on hospital surfaces and devices confer high antibiotic resistance and favors horizontal gene transfer of genetic-resistant mobile elements, the spreading of infections, and relapses. Here, we genotypically and phenotypically characterized 100 clinically isolated S. aureus for their resistance to 18 antibiotics (33% of them were OXA resistant MRSA) and ability to form biofilms. From them, we selected 48 strains on the basis on genotype group, antimicrobial-resistance profile, and existing OXA resistance to be assayed against bacteriocin AS-48. The results showed that AS-48 was active against all strains, regardless of their clinical source, genotype, antimicrobial resistance profile, or biofilm formation capacity, and this activity was enhanced in the presence of the antimicrobial peptide lysozyme. Finally, we explored the effect of AS-48 on formed S. aureus biofilms, observing a reduction in S. aureus S-33 viability. Changes in the matrix structure of the biofilms as well as in the cell division process were observed with scanning electron microscopy in both S-33 and S-48 S. aureus strains.

scholarly journals The Inhibition Potentials of Different Honey against Staphylococcus aureus, Escherichia coli and Bacillus species Isolated from Clinical Source

2020 ◽  
pp. 46-55
Author(s):  
Akani, Nedie Patience ◽  
Amadi Wemedo, Samuel ◽  
Njoku, Onyedikachi Egbuchulem
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacillus Cereus ◽  
Pathogenic Bacteria ◽  
Inhibition Efficiency ◽  
Minimum Bactericidal Concentration ◽  
Bacterial Species ◽  
Diffusion Method ◽  
Bacillus Species ◽  
Clinical Source

As a result of the increased prevalence of antibiotic resistance among different bacteria, different plants and other natural products have been studied and found to be highly effective against pathogenic bacteria. Honey, over the years has been used as an antibacterial agent to treat certain infections caused by bacteria and is believed to be effective especially in rural areas. This study was thus aimed at comparing the effect of different honey samples against some pathogenic bacteria (Escherichia coli, Staphylococcus aureus and Bacillus cereus) isolated from clinical source. This study was carried out in the microbiology laboratory, department of microbiology Rivers State University Nigeria from January 2018 to August 2019. The antibacterial sensitivity test was carried out using agar well diffusion method while the Minimum inhibitory concentration and Minimum bactericidal concentration were determined using broth tube micro dilution technique in two fold dilution. The inhibition efficiency of the honey samples on the test organisms increased with increasing concentration from 20 to 100% as 100% concentration had the highest zone of inhibition. Staphylococcus aureus (6.33 mm – 26.33 mm) was the most sensitive to the honey samples while Bacillus cereus (0.00 – 19.67 mm) was less sensitive. At concentrations of 20 – 80%, raw and Rowse honey were more effective on E. coli compared to Princenic Global honey, while at 100%, Princenic Global honey was more effective on Staphylococcus aureus. Raw and Rowse honey were more effective at 20 -60% concentrations followed by Princenic Global honey; whereas at 80 -100% concentrations, Raw and Princenic Global honey were more effective. Bacillus cereus was resistancet to the honey samples at 20 – 60% but sensitive at 80 – 100% concentrations to Rowse, Raw and Princenic Global honey. The inhibition efficiency of the honey samples on the growth of the tested organisms was found to be dependent on the concentration and type of honey used, as well as they type of organism tested. The result of the minimum inhibitory and minimum bactericidal concentration showed that Staphylococcus aureus was inhibited most at a lower concentration of 25% compared to other bacteria isolates. All honey samples tested did not show any bactericidal effect but was bacteriostatic to some of the tested organisms. Pharmacological standardization and clinical evaluation on the effect of honey is essential before honey can be used as a preventive and curative measure to common diseases related to the tested bacterial species.

Cytochemical Studies of Cell Wall Biosynthesis in Staphylococcus Aureus

1972 ◽  
Vol 30 ◽  
pp. 328-329
Author(s):  
Masaatsu Koike ◽  
Koichi Nakashima ◽  
Kyoko Iida
Keyword(s):  
Staphylococcus Aureus ◽  
Periodate Oxidation ◽  
Early Time ◽  
The Other ◽  
Penicillin G ◽  
Cell Wall Biosynthesis ◽  
Septal Wall ◽  
Peptidoglycan Biosynthesis ◽  
Gram Positive Bacteria ◽  
Cross Linkage

Penicillin exerts the activity to inhibit the peptide cross linkage between each polysaccharide backbone at the final stage of wall-peptidoglycan biosynthesis of bacteria. Morphologically, alterations of the septal wall and mesosome in gram-positive bacteria, which were occurred in early time after treatment with penicillin, have been observed. In this experiment, these alterations were cytochemically investigated by means of silver-methenamine staining after periodate oxidation, which is applied for detection of localization of wall mucopolysaccharide.Staphylococcus aureus strain 209P treated with 100 u/ml of penicillin G was divided into two aliquotes. One was fixed by Kellenberger-Ryter's OSO4 fixative at 30, 60 and 120 min after addition of the antibiotic, dehydrated through alcohol series, and embedded in Epon 812 (Specimen A). The other was fixed by 21 glutaraldehyde, dehydrated through glycolmethacrylate series and embedded in glycolmethacrylate mixture, according to Bernhard's method (Specimen B).

Gold labeling of teichoic acid on adjacent surfaces of staphylococcus aureus

1990 ◽  
Vol 48 (3) ◽  
pp. 578-579
Author(s):  
Margaret Hukee
Keyword(s):  
Staphylococcus Aureus ◽  
Teichoic Acid ◽  
Protein Labeling ◽  
Minimal Amount ◽  
Section Thickness ◽  
Double Labeling ◽  
Parallel Surfaces ◽  
Supporting Membrane

Gold labeling of two antigens (double labeling) is often done on two section surfaces separated by section thickness. Whether labeling is done on both sides of the same section or on two parallel surfaces separated by section thickness (PSSST), comparable results are dependent on an equal number of epitopes being exposed at each surface. We propose a method to study protein labeling within the same field of proteins, by examining two directly adjacent surfaces that were split during sectioning. The number of labeling sites on adjacent surfaces (AS) were compared to sites on PSSST surfaces in individual bacteria.Since each bacteria needed to be recognizable in all three section surfaces, one-hole grids were used for labeling. One-hole grids require a supporting membrane and excessive handling during labeling often ruptures the membrane. To minimize handling, a labeling chamber was designed that is inexpensive, disposable, minimizes contamination, and uses a minimal amount of solution.

Post-operative Staphylococcus aureus infection of a superficial femoral artery stent

VASA ◽  
2013 ◽  
Vol 42 (5) ◽  
pp. 382-386
Author(s):  
Karim Gariani ◽  
Marc Righini ◽  
Marco Roffi ◽  
Gino Gemayel ◽  
Damiano Mugnai ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Femoral Artery ◽  
Superficial Femoral Artery ◽  
Aureus Infection
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