Microbiota in the Rhizosphere of Cereal Crops

2021 ◽  
Vol 83 (1) ◽  
pp. 21-31
Author(s):  
V.P. Karpenko ◽  
◽  
S.P. Poltoretskyi ◽  
V.V. Liubych ◽  
D.M. Adamenko ◽  
...  

Today, spelt wheat grain is used to produce high quality food. Intermediate wheatgrass is a promising crop for prairie restoration. One of the elements of biologization is the influence of growing crops on the microbiota of soil rhizosphere. The microbiota of spelt wheat and intermediate wheatgrass soil rhizosphere remains insufficiently studied. Aim. To study the number of individual groups of microbiota in dynamics in the rhizosphere of cereal crops (spelt wheat, intermediate wheatgrass) depending on the weather conditions and the phase of plants development. Methods. Classical microbiological, statistical methods were used in the work. In particular, the study of the number of microorganisms of different ecological and trophic groups (ammonifying, nitrifying, cellulolytic and nitrogen-fixing) was carried out according to generally accepted methods in soil microbiology. The reliability of the influence of factors was determined by the probability value «р» level which was calculated using STATISTICA 8 program. Results. The amount of ammonifying and cellulolytic microorganisms in the soil rhizosphere of spelt wheat is significantly higher compared to soft wheat. The rhizosphere microbiota amount of the intermediate wheatgrass on the 2–3 year of cultivation was more resistant to adverse environmental factors compared to soft wheat. The soil rhizosphere microbiota did not change a lot depending on the phase of plant development during the vegetation period of cereal crops (spelt wheat, intermediate wheatgrass). Conclusions. The formation of rhizosphere microbiota of spelt wheat and intermediate wheatgrass was first analyzed under the conditions of the Right-Bank forest-steppe of Ukraine. The conducted studies indicate the feasibility of growing and use of spelt wheat in breeding programs to create cultivars of soft wheat with higher activity of rhizosphere microbiota. The number of ammonifying, nitrifying and cellulolytic microorganisms of soil rhizosphere of intermediate wheatgrass was significantly higher compared to soft wheat during all growth stages. The conducted studies confirm the practical application of intermediate wheatgrass to preserve and increase soil fertility. Intermediate wheatgrass can be grown for up to three years in one field, as microbiological activity reaches its maximum development.

2020 ◽  
Vol 10 (5) ◽  
pp. 81-86
Author(s):  
V.P. Karpenko ◽  
S.P. Poltoretskyi ◽  
V.V. Liubych

Estimation of spelt wheat and the species of intermediate wheatgrass by the main agrobiological indicators (calendar dates of the beginning of the main phases of plant development, plant height dynamics, dry mass accumulation, grain yield and protein content) compared to soft wheat was done. The index of stability of grain yield formation was determined. It was found that the main phases of development in spelt wheat occurred on average 10–15 days later than in soft wheat. Plants of intermediate wheatgrass in the first year of cultivation had slower growth. The main phases of development in plants of intermediate wheatgrass of the second and third year of growth occurred almost in the same time with soft wheat. It was established that spelt wheat and intermediate wheatgrass were significantly superior to soft wheat in plant height. In the earing phase, the height of these plants was on average more than 100 cm, which had to be taken into account when growing these crops. It varied from 25 to 42 cm in spelt wheat and from 29 to 57 cm in intermediate wheatgrass during the stem elongation phase depending on the weather conditions of the research year. This indicator ranged from 107 to 113 cm and from 96 to 117 cm, respectively, in the earing phase, and from 137 to 168 cm and from 107 to 196 cm in the phase of milk ripeness of grain. Plants of spelt wheat and intermediate wheatgrass had a high stability index of the formation of dry matter and grain yield. It should be noted that intermediate wheatgrass formed a significant vegetative mass even in the earing phase. The vegetative mass in spelt wheat was formed during the earing period – full ripeness of grain. These crops (spelt wheat – 22.1 ± 0.4, intermediate wheatgrass – 22.4–24.6 ± 0.2–0.5) were significantly superior to soft wheat (12.1 ± 0.5) by the content of protein in grain, so it was recommended to involve them in the selection programs to create species with high productivity. However, spelt wheat (5.58 ± 0.13) was less and intermediate wheatgrass (0.98–1.22 ± 0.14–0.18) was the most inferior to soft wheat (8.03 ± 0.27) in terms of grain yield.


2020 ◽  
pp. 160-168
Author(s):  
I. Senyk

Botanical composition of grasses is one of the most important indicators the biological value and quality of the obtained hay and pasture forage, the longevity of hayfi elds and pastures depend on. The issue of changing the botanical composition of agrophytocenoses is especially important in the context of global climate change, which in recent decades is also manifested in the territory of Ukraine, as it is possible to establish the most adapted species of legumes and cereals to adverse weather conditions and to identify eff ective technological methods of managing these processes for maximum conservation economically valuable species in the herbage. The purpose of the research is to establish the infl uence of diff erent ways of sowing of clover and alfalfa cereal crops agrophytocenoses on the formation of their botanical composition. Field studies have established diff erent eff ects of conventional in-line, cross-section and cross-sectional methods of sowing on the formation of botanical composition of grass mixtures of clover meadow (Trifolium pratense) varieties Sparta and Pavlyna with timothy meadow (Phleum pratense) and fenugreek multifl oral (Lolium multifl orum) and of agrophytocenoses of alfalfa of Sinyukha and Seraphima sowing varieties with reed fire (Festuca arundinacea Schreb) and middle wheatgrass (Elytrigia intermedia). For the average of four years of life of clover and alfalfa cereal crops agrophytocenoses, the highest proportion of legume component was observed with split-cross sowing – 51.6 % for Sparta, 53.1 % for Pavlyna, 60.3 % for Seraphima and 61.6 % for the Sinyukha variety. In the fourth year of life (the third year of use) of sowed leguminous-cereals agrophytocenoses, the preservation of the legume component was 14.6–15.5 % in clover-cereals grass mixtures with the Sparta variety and 16.0–16.8 % with the Pavlyna variety. In alfalfa grasslands, these indicators were 54.0–55.1 % with Seraphim and 55.0–56.2 % with Sinyukha. Among the studied varieties of clover meadow and alfalfa sowing proved better in the conditions of the Forest Steppe of western Pavlyna and Sinyukha. Cross-sectional and divided cross-sectional sowing of legumes and cereals mixtures proved to be better compared to conventional row crops in terms of conservation of economically valuable grass species. Key words: agrophytocenosis, botanical composition, clover meadow, alfalfa sowing, sowing methods.


2010 ◽  
Vol 3 (1) ◽  
pp. 12-16 ◽  
Author(s):  
Matthew J. Rinella ◽  
Marshall R. Haferkamp ◽  
Robert A. Masters ◽  
Jennifer M. Muscha ◽  
Susan E. Bellows ◽  
...  

AbstractAuxinic herbicides, such as 2,4-D and dicamba, that act as plant growth regulators are commonly used for broadleaf weed control in cereal crops (e.g., wheat, barley), grasslands, and noncroplands. If applied at late growth stages, while cereals are developing reproductive parts, the herbicides can reduce seed production. We tested whether growth regulators have this same effect on the invasive annual grass Japanese brome. The herbicides 2,4-D, dicamba, and picloram were applied at typical field use rates to Japanese brome at various growth stages in a greenhouse. Picloram reduced seed production nearly 100% when applied at the internode elongation, boot, or heading stages of growth, whereas dicamba appeared to be slightly less effective and 2,4-D was much less effective. Our results indicate it may be possible to control Japanese brome by using growth regulator herbicides to reduce its seed production, thereby depleting its short-lived seed bank.


2018 ◽  
Vol 12 (3) ◽  
pp. 39-45
Author(s):  
Людмила Плотникова ◽  
Lyudmila Plotnikova ◽  
Айнура Сагындекова ◽  
Aynur Sagyndekova ◽  
Галина Бережкова ◽  
...  

In Western Siberia, the resistance to septoria was tested for samples of wheatgrass of elongated Agropyron elongatum and introgressive lines of spring soft wheat with its genes, created at Omsk State Agrarian University. The experiments were conducted in the field in the forest-steppe zone of the south of Western Siberia (Omsk) in 2013-2016 on an annual natural infectious background. In 2013, the manifestations of septoriosis were weak, in 2014-2015 - moderate, and in 2016 epiphytoty was spread, which led to a severe defeat of varieties of West Siberian selection. A. elongatum samples showed immunity to the disease. In 2013, 15 promising wheat lines were identified that showed high or medium resistance to the Septoria tritici fungus, causing leaf spotting, and Parastagonospora nodorum, which affects leaves and spikes. The lines were predominantly in the middle or middle groups. According to the results of four-year experiments in contrasting weather conditions, the best lines on average showed higher productivity than, or comparable to, the standard varieties, mainly due to the increased number of productive stems of the plant and a high mass of 1000 grains. During the period of observation, there was an increase in the damage of wheat varieties and lines by the causative agents of Septoria, especially S. tritici. In 2013, 8 lines showed high resistance to leaf damage, 10 lines - ears. During the epiphytosis of 2016 year the resistance to S. tritici retained only 2 lines. The resistance to damage to the ear was more stable, in 2016, 6 lines were high, and 5 - medium resistant to P. nodorum. Two lines showed high resistance to septoriosis of leaves and ear. Dedicated lines are recommended for use in breeding soft wheat for resistance to disease.


2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Sophie Held ◽  
Catrin E. Tyl ◽  
George A. Annor

Cold plasma is an emerging technology to improve microbiological safety as well as functionality of foods. This study compared the effect of radio frequency cold plasma on flour and dough properties of three members of the Triticeae tribe, soft as well as hard wheat (Triticum aestivum L.) and intermediate wheatgrass (Thinopyrum intermedium, IWG). These three flour types differ in their protein content and composition and were evaluated for their solubility, solvent retention capacity, starch damage, GlutoPeak and Farinograph profiles, and protein secondary structures. Plasma treatment resulted in dehydration of flours but did not change protein content or solubility. Farinograph water absorption increased for all flours after plasma treatment (from 56.5–61.1 before to 71.0–81.6%) and coincided with higher solvent retention capacity for water and sodium carbonate. Plasma treatment under our conditions was found to cause starch damage to the extent of 3.46–6.62% in all samples, explaining the higher solvent retention capacity for sodium carbonate. However, Farinograph properties were changed differently in each flour type: dough development time and stability time decreased for hard wheat and increased for soft wheat but remained unchanged in intermediate wheatgrass. GlutoPeak parameters were also affected differently: peak torque for intermediate wheatgrass increased from 32 to 39.5 GlutoPeak units but was not different for the other two flours. Soft wheat did not always aggregate after plasma treatment, i.e., did not aggregate within the measurement time. It was also the only flour where protein secondary structures were changed after plasma treatment, exhibiting an increase from 15.2 to 27.9% in β-turns and a decrease from 59.4 to 47.9% in β-sheets. While this could be indicative of a better hydrated gluten network, plasma-treated soft wheat was the only flour where viscoelastic properties were changed and extensibility decreased. Further research is warranted to elucidate molecular changes underlying these effects.


2018 ◽  
Vol 12 (4) ◽  
pp. 61-66
Author(s):  
Владимир Шаманин ◽  
Vladimir Shamanin ◽  
Инна Потоцкая ◽  
Inna Potockaya ◽  
Олег Кузьмин ◽  
...  

In connection with climate warming and, consequently, the deterioration of phytopathological situation, associated with the emergence of aggressive races of fungal diseases, as well as the cultivation of susceptible varieties on the main wheat sowing area in Western Siberia, it is important to expand the genotypic variety of wheat varieties and to search for new sources of long-term sustainability. The results of the assessment of spring soft wheat varieties of the KASIB breeding nursery, set up jointly by the scientific institutions of Western Siberia, Kazakhstan and CIMMYT International Center for the Improvement of Corn and Wheat are presented in the conditions of the southern forest-steppe of West Siberia. The research was conducted in 2011-2016, using field and laboratory methods. The results of the assessment of susceptibility of KASIB varieties to brown and stem rust have shown, that varieties with high resistance prevail among resistant varieties in all phases of plant development, which indicates targeted selection for immunity with genes of race-specific and age resistance. The use of cluster analysis made it possible to identify significant differences in productivity and resistance to rust diseases of the varieties of KASIB program. Wheat varieties from KASIB are underlined: 14, 15 - Lutestsens 141/03-2 and Sigma (SibNIISKh), from KASIB nursery 16, 17 - Erytrospermum 85-08 (Omsk SAU), Lutestsens 6/04-4 and Lutestsens 186/04- 61 (SibNIISKh), resistant to brown and stem rust, exceeding in terms of yield standards, which are recommended to be used as a starting material for wheat selection in Western Siberia.


2021 ◽  
Vol 215 (12) ◽  
pp. 9-18
Author(s):  
Lidiya Mal'ceva ◽  
Natal'ya Bannikova ◽  
Elena Filippova

Abstract. The purpose of the research is the reaction of spring soft wheat varieties to dry phenomena during the growing season in the forest-steppe zone of the Trans-Urals. Methods. The material was 24 varieties of various biotypes for maturation from the nursery of the competitive variety testing of the Kurgan Research Institute of Agricultural Sciences. Results. The negative effect of the drought reduced the yield in the experiments by 11.9 c/ha, the weight of 1000 grains by 5.7 g, and the nature by 19.7 g/l. The influence of grain size on the yield both in favorable years and in years of drought was noted (r = 0.63…0.98). In arid conditions, vitreous is correlated with yield, 1000 grain weight, in kind, to a lesser extent with gluten content, bread volume, and “flour strength”. The lack of moisture during the grain filling period increased the gluten content by 3.4 %, the quality by 15.1 e. p. IDC, the flour strength by 102.7 e. a., the volume of bread by 106 g/l. In favorable years, the weight of 1000 grains and the vitreous content of the grain correlate with gluten (r = 0.44; r = 0.24–0.84). Indicators of productivity and physical properties of grain increase when sowing at the optimal time, with a certain decrease in the gluten content (by 1.2 %), flour strength (by 5.3 e. a.), bread volume (by 73.2 e. p.). In the early sowing period, moisture deficiency reduces the indicators for all varieties Under these conditions, early-maturing varieties form a larger grain both when sowing at an early time (29.0 g) and when sowing at the optimal (31.3 g). In a drought, the role of the variety is especially high. High drought resistance was shown by the Zauralochka variety of the Kurgan Research Institute of Agriculture, which successfully passed the test in the dry years (2012, 2020) in the GSI system and in the environmental test (KASIB). The yield of the drought-resistant variety Zauralochka on average for the HSU was 20.2 c/ha, exceeding the standard Hercules by 2.2 c/ha. Scientific novelty. The features of the influence of dry phenomena on the yield and the main indicators of the quality of varieties are revealed, the correlation between them is determined. According to drought resistance, a variety of spring soft wheat Zauralochka was selected.


2021 ◽  
Vol 16 (4) ◽  
pp. 21-26
Author(s):  
Elena Demina ◽  
Aleksandr Kincharov ◽  
Tat'yana Taranova ◽  
Ol'ga Mullayanova ◽  
Kristina Chekmasova

The work was carried out in 2018-2019 in the forest-steppe zone of Samara region. The purpose of the research is to conduct study samples of collection nursery of various ecological and geographical origins and identify new sources of economically valuable traits for breeding in the forest – steppe of the Middle Volga region. The material for research was 352 collection samples of spring soft wheat, including 108 foreign varieties and hybrids and 244 domestic samples of breeding institutions of the Russian Federation. Meteorological conditions during the research years were quite severe, and vegetation took place in arid and acutely arid conditions (hydrothermal coefficient in 2018 – 0.51, in 2019 – 0.48, with a long-term value in the region of 0.73). The greatest variability over the years of research was observed in the indicators of grain yield of samples (Сv=23.9...27.5 %) and plants height (Сv=10.8...12.9 %). According to the research results, 30 new genetic sources of economically valuable traits of spring soft wheat were identified. Sources of precocity (period of shoots-earing 35...37 days): Uralskaya kukushka, Chelyaba rannyaya, Tyumenskaya 25, Lutescens 70, Odeta, Libertina, Chi Mai, Long Fu 7, M83-1551. Sources of short stems (plants height 40...45 cm): KWS Torridon, KWS Jetstream, Florens, Eleganza, Long Fu 13. Sources of consistently high productivity (grain yield 415...554 g/m2, and the yield increase over the standard 71...210 g/m2): Erythrospermum 4089, Grekum 5523, Lutescens 6029, Lutescens 6074/6-23, Lutescens 6102/1-32, Lutescens 6102/1-34 Erythrospermum 6517/24-1, Ekada 214, Burlak, Ulyanovskaya 105, Lebedushka, Saratovskaya 73. Sources of high protein content (15.4...16.7 %) and gluten (36.0...42.0 %) in grain: Erythrospermum 3898, Erythrospermum 4112, Erythrospermum 4171, Erythrospermum 5289


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