Proposals for the ISS: «Plant Tumors» Experiment Study of microgravity effects on tumor formation in plants by the model of crown gall induction with agrobacterium tumefaciens

2000 ◽  
Vol 6 (4) ◽  
pp. 99-99
Author(s):  
V.V. Sarnatzkaya
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Crown Gall ◽  
Tumor Formation ◽  
Experiment Study ◽  
Plant Tumors ◽  
Gall Induction

Untersuchungen an Crown-Gall / Studies on Crown-Gall

1970 ◽  
Vol 25 (12) ◽  
pp. 1458-1460 ◽  
Author(s):  
Rolf Beiderbeck
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Visible Light ◽  
Host Cell ◽  
Crown Gall ◽  
Gall Induction

From Agrobacterium tumefaciens, strain B6, a clone B6Ri could be isolated which grows well in presence of 150 ug/ml rifampicin. Moreover B6Ri does not alter the rifampicin in the medium (quantity, spectrum in visible light, ability to inhibit the growth of B6). B6Ri is able to induce crown-galls on leaves of Kalanchoe in the presence of 100 µg/ml rifampicin. Therefore the rifampicin-mediated inhibition of crown-gall induction by B6 is not a consequence of any influence of the drug on the host cell

Untersuchungen an Crown-Gall

1970 ◽  
Vol 25 (7) ◽  
pp. 735-738 ◽  
Author(s):  
Rolf Beiderbeck
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Crown Gall ◽  
Tumor Formation ◽  
Tumor Induction ◽  
Host System ◽  
Induced Tumors ◽  
Kalanchoë Daigremontiana ◽  
Kalanchoe Daigremontiana

Concentrations of 10 µg/ml rifampicin reduce the growth of Agrobacterium tumefaciens by more than 90%, concentrations of 100 µg/ml prevent tumor formation in Kalanchoë daigremontiana by the same bacteria.A treatment with rifampicin does not irreversibly alter the bacteria; after removal of the drug bacterial propagation and the ability of tumor induction are restored.0-24 h treatments of the plant wounds with rifampicin prior to infection prevent tumor formation completely.Addition of rifampicin after infection inhibits tumor formation optimally if the drug is added only few hours after inoculation. 30 h after infection the bacteria-host-system is resistant against rifampicin.100 μg/ml rifampicin do not influence the growth of already induced tumors.

The effects of salicylates on phenomena related to crown gall

1984 ◽  
Vol 62 (4) ◽  
pp. 729-734 ◽  
Author(s):  
B. Saint-Pierre ◽  
L. Miville ◽  
P. Dion
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Mutant Strain ◽  
Crown Gall ◽  
Tumor Tissue ◽  
Tumor Formation ◽  
Crown Gall Disease ◽  
Low Concentrations ◽  
Rate Of Photosynthesis ◽  
Ph Dependent ◽  
High Concentrations

The effects of acetylsalicylate and salicylate were investigated on three phenomena involved in crown-gall disease. First, an inhibition of the growth of Agrobacterium tumefaciens was observed with low concentrations of salicylates. This inhibition was pH dependent and was partially reversed by glutamate. Second, an inhibition of the growth of tobacco tumor tissue was observed only at high concentrations of salicylates. Third, tumor formation on excised tobacco leaves was inhibited by low concentrations of salicylates. The inhibition of tumor formation occurred at salicylate concentrations that had no significant effect on the net rate of photosynthesis by the leaves. The inhibition of tumor formation was also observed with a mutant strain of A. tumefaciens resistant to salicylate. The potential interest of salicylates as control agents for crown-gall disease is discussed.

scholarly journals Identification and Characterization of a Pseudomonas mosselii strain and its Antibacterial Function against Agrobacterium tumefaciens

2021 ◽  
Vol 4 (2) ◽  
pp. 223-231
Author(s):  
Zhilong Wang ◽  
◽  
Wen Li ◽  
Jiaying Wang ◽  
Xintong Jiang ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Crown Gall ◽  
Biochemical Characterization ◽  
Inhibition Zone ◽  
Tumor Formation ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Cherry Tree ◽  
Bacterial Colony ◽  
Crown Gall Disease

Agrobacterium tumefaciens is a gram negative bacterial that can infect a range of plants and result in root crown gall. A total number of 10328 bacterial strains were isolated from rhizosphere of cherry tree. One strain of LWB10 showed clear inhibition zone around the bacterial colony in YEB media inoculated with A. tumefaciens C58. Morphological, physiological, and biochemical characterization indicated that LWB10 belongs to member of the genus Pseudomonas. Results from the high-throughput matrix-assisted laser desorption/ionization biotypersmart system indicated that this strain had a score value of 2.247 relative to Pseudomonas mosselii. Also, phylogenetic analysis based on 16S rRNA gene sequence showed that strain LWB10 shared the highest similarity with Pseudomonas mosselii CIP 105259T. The antagonist strains also exhibit well in growth inhibition of other five A. tumefaciens strains. Coinoculation of LWB10 and plant pathogenic strain of A. tumefaciens CFCC1369 showed strongly inhibition of tumor formation in tomato stems. All the results demonstrated that the isolated strain is P. mosselii LWB10 and its antibacterial ability to A. tumefaciens may offer new way for management of crown gall disease in the future. Keywords: Agrobacterium tumefaciens, Pseudomonas mosselii, antagonist, biological control

scholarly journals Agrobacterium tumefaciens Ribonucleic Acid Synthesis in Tomato Cells and Crown Gall Induction

1971 ◽  
Vol 106 (2) ◽  
pp. 634-639 ◽  
Author(s):  
M. Stroun ◽  
P. Anker ◽  
P. Gahan ◽  
A. Rossier ◽  
H. Greppin
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Ribonucleic Acid ◽  
Crown Gall ◽  
Acid Synthesis ◽  
Gall Induction
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