Development of assay method for the determination of iodine-containing organic compounds by high-performance liquid chromatography

2019 ◽  
Vol 26 (3) ◽  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Organic Compounds ◽  
High Performance ◽  
Assay Method

Specific serum quinidine assay by high-performance liquid chromatography.

1977 ◽  
Vol 23 (11) ◽  
pp. 2030-2033 ◽  
Author(s):  
W G Crouthamel ◽  
B Kowarski ◽  
P K Narang
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Raw Materials ◽  
Therapeutic Index ◽  
Assay Method ◽  
Cardioactive Drug ◽  
Double Extraction ◽  
Serum Quinidine

Abstract The cardioactive drug quinidine has a narrow therapeutic index; consequently, determination of serum quinidine concentrations can be important. We describe a relatively rapid and specific assay for quinidine in serum by high-performance liquid chromatography. It is suitable for use in patient monitoring or pharmacodynamic studies. Alkalinized serum is extracted with benzene, which is evaporated under nitrogen and reconstituted with methanol; an aliquot is chromatographed. Quinidine is separated from its metabolites and dihydroquinidine (a contaminant in quinidine raw materials). The retention time for quinidine is 4 min 10 s, for dihydroquinidine it is 5.5 min. Results for patients' sera by this assay method and the double-extraction method of Cramer and Isaksson [Scand. J. Clin. Lab. Invest. 15, 553 (1963] correlate well (r = .975).

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