scholarly journals Peculiarities of Culture and In vitro Contact Interaction of Cryopreserved Thymic Multipotent Stromal Cells and Hemopoietic Cells

2018 ◽  
Vol 28 (1) ◽  
pp. 005-013 ◽  
Author(s):  
Kateryna I. Nikolska ◽  
Keyword(s):  
Contact Interaction ◽  
Stromal Cells ◽  
Multipotent Stromal Cells ◽  
Hemopoietic Cells

scholarly journals Experimental Type 2 Diabetes Differently Impacts on the Select Functions of Bone Marrow-Derived Multipotent Stromal Cells

Cells ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 268
Author(s):  
Jonathan Ribot ◽  
Cyprien Denoeud ◽  
Guilhem Frescaline ◽  
Rebecca Landon ◽  
Hervé Petite ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Bone Marrow ◽  
Stromal Cells ◽  
Adipogenic Differentiation ◽  
Therapeutic Modality ◽  
Multipotent Stromal Cells ◽  
Cell Therapies

Bone marrow-derived multipotent stromal cells (BMMSCs) represent an attractive therapeutic modality for cell therapy in type 2 diabetes mellitus (T2DM)-associated complications. T2DM changes the bone marrow environment; however, its effects on BMMSC properties remain unclear. The present study aimed at investigating select functions and differentiation of BMMSCs harvested from the T2DM microenvironment as potential candidates for regenerative medicine. BMMSCs were obtained from Zucker diabetic fatty (ZDF; an obese-T2DM model) rats and their lean littermates (ZL; controls), and cultured under normoglycemic conditions. The BMMSCs derived from ZDF animals were fewer in number, with limited clonogenicity (by 2-fold), adhesion (by 2.9-fold), proliferation (by 50%), migration capability (by 25%), and increased apoptosis rate (by 2.5-fold) compared to their ZL counterparts. Compared to the cultured ZL-BMMSCs, the ZDF-BMMSCs exhibited (i) enhanced adipogenic differentiation (increased number of lipid droplets by 2-fold; upregulation of the Pparg, AdipoQ, and Fabp genes), possibly due to having been primed to undergo such differentiation in vivo prior to cell isolation, and (ii) different angiogenesis-related gene expression in vitro and decreased proangiogenic potential after transplantation in nude mice. These results provided evidence that the T2DM environment impairs BMMSC expansion and select functions pertinent to their efficacy when used in autologous cell therapies.

scholarly journals Intra-Vitreal Administration of Microvesicles Derived from Human Adipose-Derived Multipotent Stromal Cells Improves Retinal Functionality in Dogs with Retinal Degeneration

2019 ◽  
Vol 8 (4) ◽  
pp. 510
Author(s):  
Anna Cislo-Pakuluk ◽  
Agnieszka Smieszek ◽  
Natalia Kucharczyk ◽  
Peter G.C. Bedford ◽  
Krzysztof Marycz
Keyword(s):  
Oxidative Stress ◽  
Retinal Degeneration ◽  
Stromal Cells ◽  
Clinical Studies ◽  
Stress Conditions ◽  
In Vitro Assays ◽  
Multipotent Stromal Cells ◽  
Mitochondrial Potential ◽  
And Oxidative Stress

This study was designed to determine the influence of microvesicles (MVs) derived from multipotent stromal cells isolated from human adipose tissue (hASCs) on retinal functionality in dogs with various types of retinal degeneration. The biological properties of hASC-MVs were first determined using an in vitro model of retinal Muller-like cells (CaMLCs). The in vitro assays included analysis of hASC-MVs influence on cell viability and metabolism. Brain-derived neurotrophic factor (BDNF) expression was also determined. Evaluation of the hASC-MVs was performed under normal and oxidative stress conditions. Preliminary clinical studies were performed on ten dogs with retinal degeneration. The clinical studies included behavioral tests, fundoscopy and electroretinography before and after hASC-MVs intra-vitreal injection. The in vitro study showed that CaMLCs treated with hASC-MVs were characterized by improved viability and mitochondrial potential, both under normal and oxidative stress conditions. Additionally, hASC-MVs under oxidative stress conditions reduced the number of senescence-associated markers, correlating with the increased expression of BDNF. The preliminary clinical study showed that the intra-vitreal administration of hASC-MVs significantly improved the dogs’ general behavior and tracking ability. Furthermore, fundoscopy demonstrated that the retinal blood vessels appeared to be less attenuated, and electroretinography using HMsERG demonstrated an increase in a- and b-wave amplitude after treatment. These results shed promising light on the application of cell-free therapies in veterinary medicine for retinal degenerative disorders treatment.

scholarly journals In vitro differentiation of human skin-derived multipotent stromal cells into putative endothelial-like cells

2012 ◽  
Vol 12 (1) ◽  
pp. 7 ◽  
Author(s):  
Radhakrishnan Vishnubalaji ◽  
Muthurangan Manikandan ◽  
May Al-Nbaheen ◽  
Balamuthu Kadalmani ◽  
Abdullah Aldahmash ◽  
...  
Keyword(s):  
Human Skin ◽  
Stromal Cells ◽  
Multipotent Stromal Cells ◽  
In Vitro Differentiation

Carrageenan hydrogel as a scaffold for skin-derived multipotent stromal cells delivery

2018 ◽  
Vol 33 (3) ◽  
pp. 422-434 ◽  
Author(s):  
Michele Patricia Rode ◽  
Addeli Bez Batti Angulski ◽  
Felipe Azevedo Gomes ◽  
Maiara Marques da Silva ◽  
Talita da Silva Jeremias ◽  
...  
Keyword(s):  
Human Skin ◽  
Delivery System ◽  
Stromal Cells ◽  
Kappaphycus Alvarezii ◽  
Skin Wound ◽  
Multipotent Stromal Cells ◽  
Skin Wound Healing ◽  
Matrix Deposition ◽  
Initial Recovery

Carrageenan is a thermoreversible polymer of natural origin widely used in food and pharmaceutical industry that presents a glycosaminoglycan-like structure. Herein, we show that kappa-type carrageenan extracted by a semi-refined process from the red seaweed Kappaphycus alvarezii displayed both chemical and structural properties similar to a commercial carrageenan. Moreover, both extracted carrageenan hydrogel and commercial carrageenan hydrogel can serve as a scaffold for in vitro culture of human skin-derived multipotent stromal cells, demonstrating considerable potential as cell-carrier materials for cell delivery in tissue engineering. Skin-derived multipotent stromal cells cultured inside the carrageenan hydrogels showed a round shape morphology and maintained their growth and viability for at least one week in culture. Next, the effect of the extracted carrageenan hydrogel loaded with human skin-derived multipotent stromal cells was evaluated in a mouse model of full-thickness skin wound. Macroscopic and histological analyses revealed some pointed ameliorated features, such as reduced inflammatory process, faster initial recovery of wounded area, and improved extracellular matrix deposition. These results indicate that extracted carrageenan hydrogel can serve as a scaffold for in vitro growth and maintenance of human SD-MSCs, being also able to act as a delivery system of cells to wounded skin. Thus, evaluation of the properties discussed in this study contribute to a further understanding and specificities of the potential use of carrageenan hydrogel as a delivery system for several applications, further to skin wound healing.

Angiogenic Potential of Multipotent Stromal Cells from the Umbilical Cord: an In Vitro Study

2016 ◽  
Vol 161 (1) ◽  
pp. 141-149
Author(s):  
I. V. Arutyunyan ◽  
E. Yu. Kananykhina ◽  
T. Kh. Fatkhudinov ◽  
A. V. El’chaninov ◽  
A. V. Makarov ◽  
...  
Keyword(s):  
Umbilical Cord ◽  
Stromal Cells ◽  
In Vitro Study ◽  
Vitro Study ◽  
Multipotent Stromal Cells ◽  
Angiogenic Potential

The Globoseries Glycosphingolipid SSEA-4 Is a Marker of Bone Marrow-Derived Clonal Multipotent Stromal Cells In Vitro and In Vivo

2013 ◽  
Vol 22 (9) ◽  
pp. 1387-1397 ◽  
Author(s):  
Michael Rosu-Myles ◽  
Jennifer McCully ◽  
Joel Fair ◽  
Jelica Mehic ◽  
Pablo Menendez ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Multipotent Stromal Cells

Integrin expression and integrin-mediated adhesion in vitro of human multipotent stromal cells (MSCs) to endothelial cells from various blood vessels

2010 ◽  
Vol 341 (1) ◽  
pp. 147-158 ◽  
Author(s):  
Julie A. Semon ◽  
Lauren H. Nagy ◽  
Claire B. Llamas ◽  
H. Alan Tucker ◽  
Ryang Hwa Lee ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Blood Vessels ◽  
Stromal Cells ◽  
Integrin Expression ◽  
Multipotent Stromal Cells
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