scholarly journals FOXP3 gene expression value in regulatory T cells in pathogenesis of graft-versus-host disease induced with cryopreserved allogenic material

2014 ◽  
Vol 24 (4) ◽  
pp. 322-331 ◽  
Author(s):  
Anatoliy N. Goltsev ◽  
◽  
Tatuyana G. Dubrava ◽  
Yuliya A. Gayevskaya ◽  
Elena D. Lutsenko ◽  
...  
Keyword(s):  
Gene Expression ◽  
T Cells ◽  
Regulatory T Cells ◽  
Graft Versus Host Disease ◽  
Host Disease ◽  
Foxp3 Gene ◽  
Graft Versus Host

Reconstitution of Regulatory T Cells after Selective Depletion of CD25+ Host-Reactive Donor Lymphocytes from Allografts and Association with Acute Graft-Versus-Host-Disease.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 595-595
Author(s):  
Stephan Mielke ◽  
Katayoun Rezvani ◽  
Scott R. Solomon ◽  
Yasemin Kilical ◽  
Bipin N. Savani ◽  
...  
Keyword(s):  
Gene Expression ◽  
T Cells ◽  
Stem Cell ◽  
Regulatory T Cells ◽  
Graft Versus Host Disease ◽  
Foxp3 Gene ◽  
Graft Versus Host ◽  
Selective Depletion ◽  
Stem Cell Product ◽  
Donor Lymphocytes

Abstract Ex-vivo selective depletion (SD) of alloreactive donor lymphocytes by anti-CD25 immunotoxin is a novel method to reduce the severity of graft-versus-host-disease (GVHD) in elderly patients undergoing matched-sibling transplantation (Blood 2005, 106 (3), 1123–9). Nevertheless, there is concern that the concurrent removal of CD4+CD25+ regulatory T cells (Tregs) with the alloactivated CD25-expressing T cells could precipitate more GVHD if depletion of alloreacting cells were incomplete. We, therefore, studied the recovery of Tregs in 11 patients within the first 150 days after SD transplantation by surface phenotyping for CD4 and CD25 and real time PCR for forkhead protein3 (foxp3) gene, shown to be specific for characterisation of Tregs. At transplant patients received a median of 1.0 (0.5–1.5) x108/kg selectively depleted (CD25−) CD3+ T cells and a stem cell product containing 0.25 x104/kg (0.1–5.0) residual unselected CD3+ T cells. Three patients received SD-DLIs on day +60 or day +100. Low average levels of foxp3 gene expression appeared around day +30 post-transplant and remained almost stable until day +150 (Figure A). GVHD occurred in 7 patients (2 grade I and 5 grade II). Acute grade I-II GVHD was restricted to 7 patients with significantly lower foxp3 gene expression at day +30 (for one patient the earliest available sample was from day +60) (Figure B). In contrast to the relatively steady state of foxp3 gene expression average CD4+CD25+ cell numbers were highest during the first 60 days declining thereafter and suggesting the presence of relatively more effector cells in the early post-transplantation period. Chimerism analysis on selected CD4+CD25+ cells showed them to be predominantly of donor origin. These results suggest that foxp3+ Tregs delivered with the stem cell product may undergo modest expansion post-transplant. The absence of high grade aGVHD in these 11 patients probably relates to the efficient removal of host-alloreactive T cells by the SD process. Nevertheless, the occurrence of low-grade aGVHD in patients with the lowest foxp3 gene expression indicates that Tregs are also involved in GVHD protection after SD transplants. As the SD approach is at a very early clinical stage of development this possibility remains to be explored in further studies. Figure Figure

The role of regulatory T cells in graft-versus-host disease management

2020 ◽  
Vol 13 (2) ◽  
pp. 141-154 ◽  
Author(s):  
Jennifer S. Whangbo ◽  
Joseph H. Antin ◽  
John Koreth
Keyword(s):  
T Cells ◽  
Disease Management ◽  
Regulatory T Cells ◽  
Graft Versus Host Disease ◽  
Host Disease ◽  
Graft Versus Host

scholarly journals Narrow-Band Ultraviolet B Phototherapy Ameliorates Acute Graft-Versus-Host Disease of the Intestine by Expansion of Regulatory T Cells

PLoS ONE ◽  
2016 ◽  
Vol 11 (3) ◽  
pp. e0152823 ◽  
Author(s):  
Akari Hashimoto ◽  
Tsutomu Sato ◽  
Satoshi Iyama ◽  
Masahiro Yoshida ◽  
Soushi Ibata ◽  
...  
Keyword(s):  
T Cells ◽  
Regulatory T Cells ◽  
Graft Versus Host Disease ◽  
Narrow Band ◽  
Ultraviolet B ◽  
Host Disease ◽  
Graft Versus Host ◽  
Acute Graft

scholarly journals Stabilization of Foxp3 by Targeting JAK2 Enhances Efficacy of CD8 Induced Regulatory T Cells in the Prevention of Graft-versus-Host Disease

2018 ◽  
Vol 201 (9) ◽  
pp. 2812-2823 ◽  
Author(s):  
Supinya Iamsawat ◽  
Anusara Daenthanasanmak ◽  
Jessica Heinrichs Voss ◽  
Hung Nguyen ◽  
David Bastian ◽  
...  
Keyword(s):  
T Cells ◽  
Regulatory T Cells ◽  
Graft Versus Host Disease ◽  
Host Disease ◽  
Graft Versus Host

scholarly journals Extremely Low Numbers of Donor Expanded Regulatory T Cells Characterized by Expression of an Activated Phenotype Can Suppress Graft-Versus-Host Disease

2018 ◽  
Vol 24 (3) ◽  
pp. S170
Author(s):  
Sabrina Copsel ◽  
Dietlinde Wolf ◽  
Brandon Kale ◽  
Henry Barreras ◽  
Casey O. Lightbourn ◽  
...  
Keyword(s):  
T Cells ◽  
Regulatory T Cells ◽  
Graft Versus Host Disease ◽  
Host Disease ◽  
Graft Versus Host
Sign in / Sign up

Export Citation Format

Share Document