scholarly journals Green flagellate alga Myochloris J.H.Belcher & Swale: prey or predator?

Algologia ◽  
2020 ◽  
Vol 30 (3) ◽  
pp. 233-249
Author(s):  
E.N. Demchenko ◽  
◽  
G.G. Lilitskaya ◽  
Keyword(s):  
Green Flagellate

A 210 kDa protein is located in a membrane-microtubule linker at the distal end of mature and nascent basal bodies

1999 ◽  
Vol 112 (11) ◽  
pp. 1633-1644 ◽  
Author(s):  
K.F. Lechtreck ◽  
A. Teltenkotter ◽  
A. Grunow
Keyword(s):  
Basal Body ◽  
Body Position ◽  
Immunogold Electron Microscopy ◽  
Basal Bodies ◽  
Western Blots ◽  
Whole Cells ◽  
Extracellular Matrix Components ◽  
Green Flagellate ◽  
Protein Rich ◽  
Novel Protein

A monoclonal antibody raised against purified flagellar basal apparatuses from the green flagellate Spermatozopsis similis reacted with a protein of 210 kDa (p210) in western blots. The protein was partially cloned by immunoscreening of a cDNA library. The sequence encoded a novel protein rich in alanine (25%) and proline (20%), which contained regions similar to proteins of comparable amino acid composition such as extracellular matrix components or the membrane-cytoskeletal linker synapsin. Using a polyclonal antibody (anti-p210) raised against the C-terminal part of p210, it was shown that the protein was highly enriched in the basal apparatuses. Immunogold electron microscopy of isolated cytoskeletons or whole cells revealed that p210 was located in the flagellar transition region. The protein was part of the Y-shaped fibrous linkers between the doublet microtubules and the flagellar membrane, as indicated by statistical analysis of post-labeled sections using anti-centrin and anti-tubulin as controls. In premitotic cells p210 was located in a fibrous layer at the distal end of nascent basal bodies, which was perforated by the outgrowing axoneme. During deflagellation the protein remained at the basal body but we observed changes in its distribution, indicating that p210 partially moved to the tip of the basal body. p210 can be used as a marker to determine basal body position, orientation (parallel or antiparallel) and number in S. similis by indirect immunofluorescence. We suppose that p210 is involved in linking basal bodies to the plasma membrane, which is an important step during ciliogenesis.

THE CYTOSKELETON OF THE NAKED GREEN FLAGELLATE SPERMATOZOPSIS SIMILIS (CHLOROPHYTA):FLAGELLAR AND BASAL BODY DEVELOPMENTAL CYCLE1

1997 ◽  
Vol 33 (2) ◽  
pp. 254-265 ◽  
Author(s):  
Karl-Ferdinand Lechtreck ◽  
Ingo Botho Reize ◽  
Michael Melkonian
Keyword(s):  
Basal Body ◽  
Green Flagellate

The flagellar apparatus ofMesostigma viride (Prasinophyceae): Multilayered structures in a scaly green flagellate

1981 ◽  
Vol 138 (3-4) ◽  
pp. 247-258 ◽  
Author(s):  
Catherine E. Rogers ◽  
David S. Domozych ◽  
Kenneth D. Stewart ◽  
Karl R. Mattox
Keyword(s):  
Flagellar Apparatus ◽  
Multilayered Structures ◽  
Green Flagellate
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