scholarly journals Diatoms of Bryopsis plumosa (Hudson) C.Agardh (Chlorophyta, Bryopsidales) epiphyton from the Black and Aegean seas

Algologia ◽  
2019 ◽  
Vol 29 (4) ◽  
pp. 446-462
Author(s):  
L.I. Ryabushko ◽  
◽  
A.V. Bondarenko ◽  
A.G. Shiroyan ◽  
◽  
...  
Keyword(s):  
Bryopsis Plumosa

Diatoms of Bryopsis plumosa (Hudson) C. Agardh (Chlorophyta, Bryopsidales) Epiphyton from the Black and Aegean Seas

2019 ◽  
Vol 21 (4) ◽  
pp. 321-334
Author(s):  
L. I. Ryabushko ◽  
A. V. Bondarenko ◽  
A. G. Shiroyan
Keyword(s):  
Bryopsis Plumosa

Purification and characterization of a lectin, BPL-3, from the marine green alga Bryopsis plumosa

2010 ◽  
Vol 23 (4) ◽  
pp. 745-753 ◽  
Author(s):  
Jong Won Han ◽  
Kang Sup Yoon ◽  
Tatyana A. Klochkova ◽  
Mi-Sook Hwang ◽  
Gwang Hoon Kim
Keyword(s):  
Green Alga ◽  
Purification And Characterization ◽  
Bryopsis Plumosa ◽  
Marine Green Alga

scholarly journals Functional Expression and Characterization of the Recombinant N-acetyl-glucosamine/N-acetyl-galactosamine-Specific Marine Algal Lectin BPL3

2017 ◽  
Author(s):  
Hyun-ju Hwang ◽  
Jin-Woo Han ◽  
Gwang Hoon Kim ◽  
Jong Won Han
Keyword(s):  
Expression System ◽  
Functional Expression ◽  
Binding Activity ◽  
Dilution Method ◽  
Carbohydrate Binding ◽  
Repeat Structure ◽  
Weak Binding ◽  
Bryopsis Plumosa ◽  
Repeated Structure ◽  
Marine Algal

Lectins, characterized by their carbohydrate-binding ability, have an extensive practical application. However, their industrial use is limited by low yields, and few active recombinant lectins have been reported. In this study, the algal lectin BPL-3 (Bryopsis plumosa lectin 3) was successfully produced using a bacterial expression system, BL21(DE3), with an artificial repeated structure (dimeric construct). Recombinant dimeric BPL3 (rD2BPL3) was confirmed by LC-MS/MS spectrometry. Expression efficiency was greater for the construct with the repeat structure (rD2BPL3) than the monomeric form (rD1BPL3). Optimal conditions for expression were 1 mM IPTG at 20 °C. Recombinant lectin was purified under denaturing conditions and refolded by the flash dilution method. Recombinant BPL3 was solubilized in 1× PBS containing 2 M urea. rD2BPL3 showed strong hemagglutination activity using human erythrocytes, similar to that of native BPL3. rD2BPL3 had a similar sugar specificity to that of the native protein, i.e., to N-acetyl-glucosamine (GlcNAc) and N-acetyl-galactosamine (GalNAc). Glycan array results showed that recombinant BPL3 and native BPL3 exhibited different binding properties. Both showed weak binding activity to α-Man-Sp. Native BPL3 showed strong binding specificity to the alpha conformation of amino sugars, and rD2BPL3 had binding activity to the beta conformation. The process developed in this study was suitable for the quality-controlled production of high amounts of soluble recombinant lectins.

PURIFICATION AND CHARACTERIZATION OF A LECTIN, BRYOHEALIN, INVOLVED IN THE PROTOPLAST FORMATION OF A MARINE GREEN ALGA BRYOPSIS PLUMOSA (CHLOROPHYTA) 1

2006 ◽  
Vol 0 (0) ◽  
pp. 060609080250009-??? ◽  
Author(s):  
Gwang Hoon Kim ◽  
Tatyana A. Klochkova ◽  
Kang-Sup Yoon ◽  
Yoon-Sup Song ◽  
Key Pyoung Lee
Keyword(s):  
Green Alga ◽  
Protoplast Formation ◽  
Purification And Characterization ◽  
Bryopsis Plumosa ◽  
Marine Green Alga

Behavior of nuclei during zoosporogenesis in Bryopsis plumosa (Bryopsidales, Chlorophyta)

2005 ◽  
Vol 118 (1) ◽  
pp. 43-48 ◽  
Author(s):  
Bunji Minamikawa ◽  
Takahiro Yamagishi ◽  
Tasuku Hishinuma ◽  
Shigeru Ogawa
Keyword(s):  
Bryopsis Plumosa

Negative Phototropism in the Rhizoid of Bryopsis plumosa

1995 ◽  
Vol 36 (6) ◽  
pp. 977-982 ◽  
Author(s):  
Mineo Iseki ◽  
Makoto Mizukami ◽  
Shunji Wada
Keyword(s):  
Bryopsis Plumosa ◽  
Negative Phototropism

Positive Phototropism in the Thallus of Bryopsis plumosa

1995 ◽  
Vol 36 (6) ◽  
pp. 971-976 ◽  
Author(s):  
Mineo Iseki ◽  
Makoto Mizukami ◽  
Shunji Wada
Keyword(s):  
Bryopsis Plumosa
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