Growth and development of the organism and immunophysiological indices of blood of male F2 rats, affected by different doses of nanogermanium citrate

2017 ◽  
Vol 4 (2) ◽  
pp. 14-22 ◽  
Author(s):  
R. Fedoruk ◽  
U. Tesarivska ◽  
M. Khrabko ◽  
M. Tsap
Keyword(s):  
Growth And Development ◽  
Male Rats ◽  
Lower Content ◽  
Internal Organs ◽  
Hematological Indices ◽  
Age Related ◽  
Coeffi Cients ◽  
Group Ii ◽  
The Impact ◽  
Different Doses

Aim. To study age-related changes in the bodyweight, internal organs, and immunophysiological indices of blood for male F 2 rats, affected by different doses of nanogermanium citrate. Methods. Physiological, immunological, clinical, colorimetric, and statistical methods were applied; automatic veterinary analyzer Orphee Mythic 18 Vet (Germany) was used to determine hematological indices. Results. The decade-wise dynamics of changes in the bodyweight, the weight of heart, liver, kidneys, testicles, lungs, spleen, and weight coeffi cients of these organs at the age of 2–3 and 4–5 months demonstrated that there were intergroup dose-dependent and age-related differences in the intensity of growth and development of the organism of male rats. The highest gain of bodyweight was registered for male rates, which received 10 μg Ge/kg of bodyweight 30 days after weaning, this tendency was kept for the lower level of these indices in groups ІІІ and IV at the effect of 20 and 200 μg Ge. On days 97, 107, and 117, males of group II and IV demonstrated non-reliably lower indices of bodyweight, whereas in group III their values did not differ much from those for the control. Reliably higher indices of weight of liver and spleen were demonstrated along with their weight coeffi cients for younger males, but they were lower at the age of 4–5 months compared to the control. The blood of 2–3 m.o. group II males had reliably lower content of hemoglobin and hematocrit index as well as the tendency to the decrease in the number of erythrocytes. At the age of 4–5 months, there was an evident decrease in the number of leukocytes and their forms – lymphocytes, monocytes, and granulocytes, which was more expressed in the blood of group II and IV males compared to the control. At the impact of nanogermanium citrate (NGeC), the blood of males of experimental groups of both age periods demonstrated higher content of immunoglobulins, circulating immune complexes, hexoses, bound to proteins, and ceruloplasmin (except for group II animals at the age of 4 months). The effect of applied doses of NGeC inhibited the input of acute phase proteins into the blood which was evident in the reliably lower content of mean mass molecules at the age of 2–3 months with its staying at the level of the control group of animals at the age of 4– months. Conclusions. The biological effect of NGeC in the doses of 10, 20, and 200 μg Ge/kg of bodyweight was demonstrated in differently directed changes in the intensity of organism growth and development, specifi c internal organs, and hematological indices, which was more expressed for animals, receiving 10 μg Ge, as well as in the differences in immunophysiological indices of blood of males of the experimental groups of both age periods, which indicated the activation of immunobiological reactivity of their organism at the age of 2–3 and 4–5 months.

Paternal Δ9-Tetrahydrocannabinol Exposure Prior to Mating Elicits Deficits in Cholinergic Synaptic Function in the Offspring

2020 ◽  
Vol 174 (2) ◽  
pp. 210-217
Author(s):  
Theodore A Slotkin ◽  
Samantha Skavicus ◽  
Edward D Levin ◽  
Frederic J Seidler
Keyword(s):  
Brain Development ◽  
Choline Acetyltransferase ◽  
Drug Exposure ◽  
Brain Regions ◽  
Synaptic Function ◽  
Male Rats ◽  
High Dose ◽  
Age Related ◽  
The Impact ◽  
Ach Receptors

Abstract Little attention has been paid to the potential impact of paternal marijuana use on offspring brain development. We administered Δ9-tetrahydrocannabinol (THC, 0, 2, or 4 mg/kg/day) to male rats for 28 days. Two days after the last THC treatment, the males were mated to drug-naïve females. We then assessed the impact on development of acetylcholine (ACh) systems in the offspring, encompassing the period from the onset of adolescence (postnatal day 30) through middle age (postnatal day 150), and including brain regions encompassing the majority of ACh terminals and cell bodies. Δ9-Tetrahydrocannabinol produced a dose-dependent deficit in hemicholinium-3 binding, an index of presynaptic ACh activity, superimposed on regionally selective increases in choline acetyltransferase activity, a biomarker for numbers of ACh terminals. The combined effects produced a persistent decrement in the hemicholinium-3/choline acetyltransferase ratio, an index of impulse activity per nerve terminal. At the low THC dose, the decreased presynaptic activity was partially compensated by upregulation of nicotinic ACh receptors, whereas at the high dose, receptors were subnormal, an effect that would exacerbate the presynaptic defect. Superimposed on these effects, either dose of THC also accelerated the age-related decline in nicotinic ACh receptors. Our studies provide evidence for adverse effects of paternal THC administration on neurodevelopment in the offspring and further demonstrate that adverse impacts of drug exposure on brain development are not limited to effects mediated by the embryonic or fetal chemical environment, but rather that vulnerability is engendered by exposures occurring prior to conception, involving the father as well as the mother.

scholarly journals ORAL ADMINISTRATION OF RAW WHITE KIDNEY BEANS (PHASEOLUS VULGARIS L. VAR. BELDIA) INDUCES OVERGROWTH OF FECAL COLIFORMS AND LACTOBACILLI IN WISTAR RATS

2018 ◽  
Vol 11 (15) ◽  
pp. 68
Author(s):  
Nader Nciri ◽  
Taesub Shin ◽  
Namjun Cho
Keyword(s):  
Food Intake ◽  
Fecal Microbiota ◽  
Fecal Coliforms ◽  
Male Rats ◽  
Internal Organs ◽  
Fecal Microflora ◽  
Kidney Beans ◽  
Bean Flour ◽  
The Impact ◽  
Fecal Score

Objective: The comprehensive dynamics of fecal microbiota in response to the ingestion of toxic bean lectins or phytohemagglutinins has not been well studied. The study aimed at evaluating the gavage effects of a raw Beldia bean variety on food intake, growth performance, gastrointestinal organs, and fecal microflora in Wistar rats.Methods: Twenty young adult male rats were randomly allotted into two groups of 10 rats each: Control rats were gavaged with 300 mg of a rodent pellet flour suspension and experimental rats were orogastrically fed a dose of 300 mg Beldia bean flour suspension (BBFS). Individual food intake, body weight, and fecal score were taken daily. To assess the impact on the gut flora, fecal samples were collected every day for 10 days. All animals were sacrificed on day 10, to obtain blood and internal organs samples.Results: The results revealed that the gavage of a BBFS to rats had no marked influence on average daily of food intake and weight gain. No significant differences were found in the weights of the small intestine, spleen, liver, and thymus of rats given raw Beldia diet. The counts of coliforms and lactobacilli on pooled fecal specimens of BBFS-fed rats were increased significantly compared to controls.Conclusion: In summary, the exposure to raw Beldia beans altered the fecal microbiota, without adverse effects on animals.

The impact of age-related sub-chronic exposure to chlorpyrifos on metabolic indexes in male rats

2020 ◽  
Vol 27 (18) ◽  
pp. 22390-22399
Author(s):  
Saeed Samarghandian ◽  
Moshen Foadoddin ◽  
Mahmoud Zardast ◽  
Omid Mehrpour ◽  
Parisa Sadighara ◽  
...  
Keyword(s):  
Chronic Exposure ◽  
Male Rats ◽  
Age Related ◽  
The Impact

scholarly journals Growth and development of F1 male rat organism and its' immunophysiological activity in the period of watering with different doses of nanotechnologically and chemically synthesized germanium citrate

2016 ◽  
Vol 21 (2) ◽  
pp. 39-43 ◽  
Author(s):  
M. Khrabko ◽  
R. Fedoruk
Keyword(s):  
Body Weight ◽  
Growth And Development ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Biological Action ◽  
Acid Number ◽  
Male Rats ◽  
Male Rat ◽  
Average Mass ◽  
Different Doses

The aim of research was to study the effect of different doses of Ge citrate on the growth and development of F1 male, the activity of their immune system and the state of hemostasis under these conditions. Research performed by methods of groups and periods with using F1 male rats, which were watering during the milk-feeding period up to 120 days with Ge citrate obtained by nanotechnology method in doses 10 (I), 20 (II), 200 (III) mcg Ge/kg body weight and chemically synthesized Ge citrate in dose 2,000 (IV) mcg Ge/kg body weight. According to the research findings the most pronounced effect watering of Ge citrate on the growth and development of male rats organism was noted in dose 20 mcg Ge/kg body weight. Biological action of Ge citrate stimulated the immunophysiological activity of the organism with increasing of total Ig, molecules of average mass, sialic acid, number of white blood cells by the increasing number of lymphocytes and granulocytes, against the background of reduction of circulating immune complexes and hexoses bound to proteins. The positive dose-dependent effect of Ge citrate on the growth and development of the rats' organism and its immunophysiological and hematological parameters was noted.

scholarly journals THE REGULATORY EFFECT OF DIFFERENT DOSES OF GERMANIUM CITRATE ON PHYSIOLOGICAL AND BIOCHEMICAL PROCESSES IN THE BODY MALE F2

2017 ◽  
Vol 73 (1) ◽  
pp. 66-69
Author(s):  
M. Khrabko ◽  
R. Fedoruk ◽  
S. Kropuvka ◽  
U. Tesarivska ◽  
Keyword(s):  
The Body ◽  
Male Rats ◽  
Control Group ◽  
Regulatory Effect ◽  
Biochemical Processes ◽  
Calcium Phosphorus ◽  
Growth Activity ◽  
The Impact ◽  
Physiological And Biochemical ◽  
Different Doses

Studied the impact of prolonged watering of different amounts of citrate Ge, obtained by nanotechnology method, on the biochemical processes and antioxidant activity blood of male rats F2 1 and 2nd offspring in times of physiological and puberty. Established differently directed influence citrate Ge on the biochemical parameters bloods of animals of all ages – the increase of creatinine, phosphorus and triacylglycerol in male 4-month-old 1st offspring, while in animals 2 offspring grew albumin, calcium, phosphorus and triacylglycerols. Noted growth activity of antioxidant enzymes – catalase, SOD and GP animals 2-month-old 1st offspring, while the 4 months activity remained at the level of the control group, but males 2nd offspring grew – SOD and GP. For the watering 200 mkg Ge content hydroperoxides lipid and TBA products in the blood is reduced by 4 months of age the animals received both the first and second litters.

Role of chromium (III) in the nutrition of pigs and cattle

2016 ◽  
Vol 3 (2) ◽  
pp. 56-62
Author(s):  
R. Iskra ◽  
V. Vlizlo ◽  
R. Fedoruk
Keyword(s):  
Growth And Development ◽  
Reproductive Function ◽  
Metabolic Effect ◽  
Considerable Part ◽  
Cultural Medium ◽  
Milk Performance ◽  
Chromium Compounds ◽  
The Impact ◽  
Different Sources

The results of our studies and the data of modern literature regarding the biological role of Cr(III) compounds in conditions of their application in the nutrition for pigs and cattle are discussed. The metabolic impact of Cr(III), coming from different sources – mineral and organic compounds, obtained by chemical synthesis or a nanotechnological method (chromium citrate), as well as in the form of biocomplexes from the cultural medium of Saccharomyces cerevisiae yeasts was analyzed. The metabolic connection between the impact of Cr(III) and the biosynthesis of some hormones – insulin, cortisol – as well as the sensitivity of some tissues and organs to the effect of chromium compounds was studied. A considerable part of the review material was dedicated to the metabolic effect of Cr(III) compounds on the reproductive function of pigs and cattle and their impact on the viability of the offspring and gametes of animals. The data about the stimulating effect of Cr(III) on the growth and development of the organism of piglets and calves, meat and milk performance of these species of animals are discussed. The relevance of dosing Cr(III) in the nutrition of pigs and cattle is highlighted.

INFLUENCE OF BIOSTIMULANTS ON THE IMMUNE PROTECTION OF CHICKENS

2019 ◽  
Keyword(s):  
Growth And Development ◽  
Digestive System ◽  
Great Influence ◽  
Egg Laying ◽  
Growth Spurt ◽  
Immune Protection ◽  
Internal Organs ◽  
State Growth ◽  
Active Peak

Among the animals of different species chickens react in greater numbers and more noticeable to a va-riety of growth biostimulators. There are great improvements on their general state, growth spurts and development of internal organs is quicker when they are injected with small portions. Such stimulation has a great influence not only on growth and development of chickens in their first period of life but also on health and productivity later on. The most active peak of reaction is when chicken is two months old. Their internal organs, especially the digestive system, develop earlier, their genitals appear earlier and they begin egg-laying much earlier too, when chickens are being fed those biostimulants. Slaughter meat yield becomes more and quality of meat improves with the influence of stimulators. A major disease pre-vention and healing effect can be reached, since most of the biostimulants raise immune system and re-sistance of the organism. It can be the only thing to justify their usage on animals. Tests show that the most typical growth spurt of birds is from fifteen to twenty percent in normal conditions. Growth spurts are also accompanied with the rise of resistance to different infections and activation of different physio-logical processes. Growth spurts can be twice or more than written here, but those spurts are usually short-timed and often accompanied with the dysfunction of different organs

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