scholarly journals Assessment of Genotoxic Damage in Nurses Occupationally Exposed to Anaesthetic Gases or Antineoplastic Drugs by the Comet Assay

2009 ◽  
Vol 51 (3) ◽  
pp. 283-286 ◽  
Author(s):  
Seval Izdes ◽  
Semra Sardas ◽  
Ela Kadioglu ◽  
Cetin Kaymak ◽  
Eren Ozcagli
Keyword(s):  
Comet Assay ◽  
Antineoplastic Drugs ◽  
Genotoxic Damage ◽  
Anaesthetic Gases ◽  
Occupationally Exposed

scholarly journals Evaluation of Genotoxic Effects of Asbestos on Occupationally Exposed Workers in Brazil

Biomonitoring ◽  
2016 ◽  
Vol 3 (1) ◽  
Author(s):  
Isabele C. Costa Amaral ◽  
Francisco J. Guimarães Joca ◽  
Leandro V. B. Carvalho ◽  
Vinicio S. Castro ◽  
Natália C. Pereira ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Comet Assay ◽  
Asbestos Exposure ◽  
Linear Regression Analysis ◽  
Exposed Group ◽  
Chrysotile Asbestos ◽  
Glutathione S Transferase ◽  
Genotoxic Effects ◽  
Genotoxic Damage ◽  
Occupationally Exposed

Abstract Genotoxic effects of occupational workers exposed to asbestos can be evaluated using different biomarkers as oxidative stress enzymes in conjuction with comet assay. This study assessed changes to oxidative stress enzymatic parameters and genotoxic damage in workers occupationally exposed and non-exposed to chrysotile asbestos, who attended the outpatient Clinic of the Center for Worker Health Studies and Human Ecology (CESTEH/ENSP/FIOCRUZ) in Brazil. Chest radiography and spirometry were performed to assess clinical progression of symptoms. The traditional visual score comet assay in peripheral whole blood cells was used to assess DNA damage, and oxidative stress was evaluated by measuring catalase (CAT) and glutathione S-transferase (GST) activities. Respiratory alterations were observed in 53% of workers exposed, as determined by pulmonary function and bronchodilation, and 6 workers were diagnosed with asbestosis. The comet assay was statistically significantly higher in the exposed group and individuals with asbestosis compared to the non-exposed group and individuals without asbestosis, respectively. Linear regression analysis showed that 28,4% and 50,5% of comet assay results were increased by exposure to asbestos and developed asbestosis. The results of CAT and GST were not difference between the groups. These results supports the association of genotoxic damage and the onset of asbestosis by chrysotile asbestos exposure in workers of this study.

Assessment of genotoxic damage in nurses occupationally exposed to antineoplastics by the analysis of chromosomal aberrations

2002 ◽  
Vol 21 (3) ◽  
pp. 129-135 ◽  
Author(s):  
S Burgaz ◽  
B Karahalil ◽  
Z Canli ◽  
F Terzioglu ◽  
G Ançel ◽  
...  
Keyword(s):  
Occupational Exposure ◽  
Population Level ◽  
Fold Increase ◽  
Mean Value ◽  
Antineoplastic Drugs ◽  
Genetic Damage ◽  
Occupationally Exposed ◽  
The Mean ◽  
Excretion Rates ◽  
Drug Handling

To estimate the genotoxic risk of occupational exposure to antineoplastic drugs, chromosomal aberration (CAs) frequencies in peripheral lymphocytes were determined for 20 nurses handling antineoplastics and 18 referents matched for age and sex. Urinary cyclophosphamide (CP) excretion rates, which are used as a marker for drug handling, were also measured on these nurses. We have observed significant frequencies of CAs (about 2.5-fold increase) including chromatid breaks, gaps, and acentric fragments for nurses handling antineoplastics as compared to control subjects (p<0.05, p<0.01, excluding and including gaps, respectively). The mean value of CP excretion rate for 12 nurses was 1.63 μg/24 h, suggesting that when the nurses handled CP (and other antineoplastic drugs) this particular compound was absorbed. Our study has shown that increased genetic damage was evident in nurses, at population level, due to occupational exposure to antineoplastics. Until the effects of handling antineoplastics from low-level exposure are known, it will be important to keep the exposure to a minimum.

Efforts to Ensure Safety of Hospital Pharmacy Personnel Occupationally Exposed to Antineoplastic Drugs During a Preparation Task

2007 ◽  
Vol 42 (3) ◽  
pp. 209-218 ◽  
Author(s):  
Kenji Ikeda ◽  
Yuriko Yagi ◽  
Manabu Takegami ◽  
Yuquan Lu ◽  
Kanehisa Morimoto ◽  
...  
Keyword(s):  
Hospital Pharmacy ◽  
Antineoplastic Drugs ◽  
Occupationally Exposed
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