Glucose sensor based on nanostructured carbon electrode with immobilized PQQ-containing glucose dehydrogenase: Construction, experimental study and mathematical modeling

2016 ◽  
Vol 21 (5) ◽  
pp. 702-715 ◽  
Author(s):  
Mantas Puida ◽  
◽  
Jurgita Dabulytė-Bagdonavičienė ◽  
Feliksas Ivanauskas ◽  
Valdemaras Razumas ◽  
...  
Keyword(s):  
Mathematical Modeling ◽  
Experimental Study ◽  
Glucose Sensor ◽  
Glucose Dehydrogenase ◽  
Carbon Electrode ◽  
Nanostructured Carbon

scholarly journals A Disposable Electrochemical Glucose Sensor Using Catalytic Subunit of Novel Thermostable Glucose Dehydrogenase

2007 ◽  
Vol 1 (1) ◽  
pp. 26-30 ◽  
Author(s):  
Hideaki Yamaoka ◽  
Koji Sode
Keyword(s):  
Blood Glucose ◽  
Whole Blood ◽  
Glucose Sensor ◽  
Glucose Dehydrogenase ◽  
Blood Glucose Measurement ◽  
Catalytic Subunit ◽  
Glucose Measurement ◽  
Carbon Electrode ◽  
Electron Mediator ◽  
Screen Printed Carbon Electrode

We report here the development of a novel disposable-type glucose enzyme sensor based on a screen-printed carbon electrode (SPCE) employing a catalytic subunit of FAD-dependent glucose dehydrogenase (FADGDH) and a Rucomplex (hexaammineruthenium (III) chloride) as the electron mediator. The whole blood glucose measurement was achieved in 2 seconds using a 500 nl sample of the range of 100-800 mg/ml.

A glucose sensor based on glucose dehydrogenase adsorbed on a modified carbon electrode

1986 ◽  
Vol 179 ◽  
pp. 371-379 ◽  
Author(s):  
György Marko-Varga ◽  
Roger Appelqvist ◽  
Lo Gorton
Keyword(s):  
Glucose Sensor ◽  
Glucose Dehydrogenase ◽  
Carbon Electrode

Mathematical modeling and experimental study of electrode processes

2014 ◽  
Vol 19 (2) ◽  
pp. 599-606 ◽  
Author(s):  
Kh. Z. Brainina ◽  
L. G. Galperin ◽  
M. A. Bukharinova ◽  
N. Yu. Stozhko
Keyword(s):  
Mathematical Modeling ◽  
Experimental Study ◽  
Electrode Processes

In Vitro Evaluation of Miniaturized Amperometric Enzyme Sensor Based on the Direct Electron Transfer Principle for Continuous Glucose Monitoring

2022 ◽  
pp. 193229682110706
Author(s):  
Yutaro Inoue ◽  
Yasuhide Kusaka ◽  
Kotaro Shinozaki ◽  
Inyoung Lee ◽  
Koji Sode
Keyword(s):  
Electron Transfer ◽  
Continuous Glucose Monitoring ◽  
Glucose Sensor ◽  
Direct Electron Transfer ◽  
Glucose Dehydrogenase ◽  
Glucose Monitoring ◽  
Reference Electrode ◽  
Enzyme Sensor

Background: The bacterial derived flavin adenine dinucleotide (FAD)-dependent glucose dehydrogenase (FADGDH) is the most promising enzyme for the third-generation principle-based enzyme sensor for continuous glucose monitoring (CGM). Due to the ability of the enzyme to transfer electrons directly to the electrode, recognized as direct electron transfer (DET)-type FADGDH, although no investigation has been reported about DET-type FADGDH employed on a miniaturized integrated electrode. Methods: The miniaturized integrated electrode was formed by sputtering gold (Au) onto a flexible film with 0.1 mm in thickness and divided into 3 parts. After an insulation layer was laminated, 3 openings for a working electrode, a counter electrode and a reference electrode were formed by dry etching. A reagent mix containing 1.2 × 10−4 Unit of DET-type FADGDH and carbon particles was deposited. The long-term stability of sensor was evaluated by continuous operation, and its performance was also evaluated in the presence of acetaminophen and the change in oxygen partial pressure (pO2) level. Results: The amperometric response of the sensor showed a linear response to glucose concentration up to 500 mg/dL without significant change of the response over an 11-day continuous measurement. Moreover, the effect of acetaminophen and pO2 on the response were negligible. Conclusions: These results indicate the superb potential of the DET-type FADGDH-based sensor with the combination of a miniaturized integrated electrode. Thus, the described miniaturized DET-type glucose sensor for CGM will be a promising tool for effective glycemic control. This will be further investigated using an in vivo study.

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