CRYSTALLOGRAPHIC ANALYSIS OF THE STRUCTURE OF SCANDIUM BOROCARBOSILICIDE. HIERARCHY IN THE HIGHLY SYMMETRICAL ORDERING OF ATOMIC GROUPS AND ATOMS

doi:10.15372/jsc20150523

Quaternary structure of Limulus polyphemus hemocyanin

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100081747 ◽

1978 ◽

Vol 36 (2) ◽

pp. 176-177

Author(s):

T. Wichertjes ◽

E.J. Kwak ◽

E.F.J. Van Bruggen

Keyword(s):

Electron Microscopy ◽

Functional Properties ◽

Horseshoe Crab ◽

Temperature Jump ◽

Quaternary Structure ◽

Crystallographic Analysis ◽

Limulus Polyphemus ◽

Oxygen Binding ◽

X Ray ◽

Intact Molecule

Hemocyanin of the horseshoe crab (Limulus polyphemus) has been studied in nany ways. Recently the structure, dissociation and reassembly was studied using electron microscopy of negatively stained specimens as the method of investigation. Crystallization of the protein proved to be possible and X-ray crystallographic analysis was started. Also fluorescence properties of the hemocyanin after dialysis against Tris-glycine buffer + 0.01 M EDTA pH 8.9 (so called “stripped” hemocyanin) and its fractions II and V were studied, as well as functional properties of the fractions by NMR. Finally the temperature-jump method was used for assaying the oxygen binding of the dissociating molecule and of preparations of isolated subunits. Nevertheless very little is known about the structure of the intact molecule. Schutter et al. suggested that the molecule possibly consists of two halves, combined in a staggered way, the halves themselves consisting of four subunits arranged in a square.

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Some Observations on Intra-Mitochondrial Crystals

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100079486 ◽

1977 ◽

Vol 35 ◽

pp. 406-407

Author(s):

J. A. Clarke ◽

D. N. Landon ◽

P. R. Ward

Keyword(s):

Cytochrome B ◽

Mitochondrial Myopathy ◽

Crystallographic Analysis ◽

Mitochondrial Membranes ◽

Electron Microscopes ◽

Muscle Biopsies

Intra-mitochondrial crystals have been noted in muscle biopsies from patients in a wide variety of diseased states. As far as we are aware, none of these crystals have been subjected to detailed crystallographic analysis. Recently, similar crystals were observed in a biopsy from a patient with a mitochondrial myopathy, characterised by a deficiency in reducible cytochrome b (Morgan-Hughes, J. A., Darveniza, P., Kahn, S. N., Landon, D. N., Sherratt, R. M., Land, J. M. and Clark, J. B., 1977, Brain, In Press). Aldehyde-fixed, osmicated resin imbedded material was examined using Siemens, JEOL and Phillips electron microscopes with goniometer specimen stages. The crystals generally lay between the outer and inner mitochondrial membranes and measured 1 - 3 μm in length and 0.1 - 0.3 μm in width. Characteristically, these crystals revealed specific periodicities.

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Morphology of Σ3{/70.53°} grain boundaries in a C15 intermetallic compound

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100171158 ◽

1994 ◽

Vol 52 ◽

pp. 684-685

Author(s):

Fuming Chu ◽

D. P. Pope ◽

D. S. Zhou ◽

T. E. Mitchell

Keyword(s):

Grain Boundary ◽

Grain Boundaries ◽

Crystallographic Analysis ◽

Laves Phase ◽

Second Phase ◽

Bright Field Image ◽

Boundary Edge ◽

Arc Melting ◽

Fcc Lattice ◽

Diffraction Patterns

A C15 Laves phase, HfV2+Nb, shows promising mechanical properties and here we describe the structure of its grain boundaries. The C15 Laves phase has a fcc lattice with a=7.4Å. An alloy of composition Hf14V64Nb22 (including a C15 matrix and a second phase of V-rich bcc solution) was made by arc-melting. The alloy was homogenized at 1200°C for 120h. Preliminary study concentrated on Σ3{<110>/70.53°} grain boundaries in the C15 phase using Philips 400T and CM 30 microscopes.The most-commonly observed morphology of Σ3{<110>/70.53°} grain boundaries in the C15 phase is a faceted boundary. A bright field image (BFI) of the faceted boundary and the corresponding diffraction patterns with the grain boundary edge-on are shown in Fig. 1(a). From the diffraction patterns using a <110> zone axis for both grains, it is obvious that this is a Σ3{<110>/70.53°} grain boundary. Crystallographic analysis shows that the Σ3{<110>/70.53°} grain boundaries selectively facet with the following relationships between the two grains: {111}1//{111}2, {112}1//{112}2, {111}1//{115}2, and {001}1//{221}2.

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A crystallographic analysis of the CoSi/Si(111) interface using Transmission Electron Microscopy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100176009 ◽

1990 ◽

Vol 48 (4) ◽

pp. 574-575

Author(s):

A.C. Daykin ◽

C.J. Kiely ◽

R.C. Pond ◽

J.L. Batstone

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Defect Structure ◽

Room Temperature ◽

Theoretical Method ◽

Crystallographic Analysis ◽

Si Substrate ◽

Transmission Electron ◽

Theoretical Predictions

When CoSi2 is grown onto a Si(111) surface it can form in two distinct orientations. A-type CoSi2 has the same orientation as the Si substrate and B-type is rotated by 180° degrees about the [111] surface normal.One method of producing epitaxial CoSi2 is to deposit Co at room temperature and anneal to 650°C.If greater than 10Å of Co is deposited then both A and B-type CoSi2 form via a number of intermediate silicides .The literature suggests that the co-existence of A and B-type CoSi2 is in some way linked to these intermediate silicides analogous to the NiSi2/Si(111) system. The phase which forms prior to complete CoSi2 formation is CoSi. This paper is a crystallographic analysis of the CoSi2/Si(l11) bicrystal using a theoretical method developed by Pond. Transmission electron microscopy (TEM) has been used to verify the theoretical predictions and to characterise the defect structure at the interface.

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