scholarly journals A PDMS Sample Pretreatment Device for the Optimization of Electrokinetic Manipulations of Blood Serum

2009 ◽  
Author(s):  
Timothy J Abram
Keyword(s):  
Blood Serum ◽  
Sample Pretreatment ◽  
Pdms Sample

First Practical Colorimetric Assay of Lithium in Serum

1992 ◽  
Vol 38 (9) ◽  
pp. 1654-1657 ◽  
Author(s):  
E Chapoteau ◽  
B P Czech ◽  
W Zazulak ◽  
A Kumar
Keyword(s):  
Solvent Extraction ◽  
Blood Serum ◽  
Therapeutic Range ◽  
High Selectivity ◽  
Colorimetric Assay ◽  
Colorimetric Method ◽  
Sample Pretreatment ◽  
Standard Curve ◽  
Flame Photometer

Abstract We report a colorimetric method for determining lithium in blood serum without sample pretreatment or solvent-extraction steps. The method is based on a novel chromogenic ionophore that exhibits exceptionally high selectivity (much greater than 4000:1) for lithium over sodium. The standard curve for the method is linear up to 3.5 mmol/L and exceeds the therapeutic range for lithium. The results for 57 patients' samples correlated well with results obtained with an Instrumentation Laboratory flame photometer (r = 0.97).

Concentration of selected micro-nutrients in saliva among women in menopausal period treated with hormone replacement therapy

2013 ◽  
Vol 22 (01) ◽  
pp. 46-51
Author(s):  
M. Rahnama ◽  
I. Jastrzêbska-Jamrogiewicz ◽  
R. Jamrogiewicz
Keyword(s):  
Bone Mineral Density ◽  
Hormone Replacement Therapy ◽  
Blood Serum ◽  
Replacement Therapy ◽  
Hormone Replacement ◽  
Surgical Removal ◽  
Femoral Bone ◽  
Mineral Density ◽  
Natural Menopause ◽  
Copper Zinc

Summary Objective: The aim of this study was to observe the variability of the level of copper, zinc and manganese in saliva amongst women with hypoestrogenia, treated and untreated with hormone replacement therapy (HRT). Methods: The study was conducted on a group of 60 women treated and untreated with HRT. Half of the patients were after natural menopause and other half was after surgical removal of ovaries. Research on micronutrients in non-stimulated saliva and blood serum was carried out in 2005. Tests on saliva were repeated in 2010. Investigation of bone mineral density (BMD) of femoral bone was performed in year 2010. Results: Statistical analysis of concentration of copper and zinc revealed a linear correlation between the levels of these microelements in blood serum and saliva. The study revealed that HRT has a beneficial effect on BMD and the concentration of copper and manganese in saliva and blood serum of patients after the menopause. Patients treated with HRT showed higher BMD values than groups not treated with hormones. Conclusions: Saliva appears to be a promising diagnostic material which can be used to analyze the content of trace elements, but further research should be carried out on a broader research group.

Antiheparin Activity of Human Serum and Platelet Factor 4

1973 ◽  
Vol 30 (01) ◽  
pp. 093-105 ◽  
Author(s):  
C.H.J Sear ◽  
L Poller ◽  
F.R.C Path
Keyword(s):  
Molecular Weight ◽  
Ionic Strength ◽  
Blood Serum ◽  
Whole Blood ◽  
Platelet Rich Plasma ◽  
Gel Filtration ◽  
Normal Serum ◽  
Platelet Factor ◽  
Mean Values ◽  
Antiheparin Activity

SummaryThe antiheparin activity of normal serum has been studied by comparing the antiheparin activities of sera obtained from normal whole blood, platelet-rich plasma and platelet-’free’ plasma with a purified platelet extract during differential isoelectric precipitation and by gel filtration chromatography.The mean values for the activity of PRP-serum and PFP-serum were 106% (S.D. 11) and 10% (S.D. 3) of untreated whole blood respectively. The activity of whole blood serum, PRP serum and whole blood serum plus platelet extract precipitated under identical physical conditions, i.e. pH 7.0, I =0.008, indicating that the activities of the three samples are probably associated with PF4. PF4 precipitated from human platelet extract at pH 4.0, but this is probably due to the difference in the two biochemical environments investigated, i.e. serum and platelet extract.The gel filtration experiments revealed striking similarities between the major antiheparin activities of serum and platelet extract. At physiological pH and ionic strength both activities were associated with high molecular weight material, but at physiological pH and elevated ionic strength both activities behaved as much smaller entities of molecular weight between 25,000 and 30,000 daltons and it seems very likely that both activities are associated with the same molecule, i.e. PF4.

Human Blood Serum Rich in Factor V

1970 ◽  
Vol 24 (03/04) ◽  
pp. 334-337 ◽  
Author(s):  
R Honorato
Keyword(s):  
Blood Serum ◽  
Human Serum ◽  
Human Blood ◽  
Human Blood Serum ◽  
Factor V ◽  
The Stability

Summary1. A technique to obtain human serum rich in factor V is described.2. Calcium increases the stability of factor V in the serum.

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