scholarly journals Effect of Shichimotsu-koka-to on lipid Peroxidation and Free-Radical-Related Enzyme Activity in Stroke-Prone Spontaneously Hypertensive Rats

1997 ◽  
Vol 38 (4) ◽  
pp. 604-604
Author(s):  
Yukito Higuchi ◽  
Kimihiro Yamashita ◽  
Kotaro Taniyama ◽  
Masayori Ozaki
Keyword(s):  
Lipid Peroxidation ◽  
Enzyme Activity ◽  
Free Radical ◽  
Spontaneously Hypertensive Rats ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Related Enzyme

Control of oxidative stress in microcirculation of spontaneously hypertensive rats

2005 ◽  
Vol 288 (2) ◽  
pp. H805-H812 ◽  
Author(s):  
F. A. DeLano ◽  
R. Balete ◽  
G. W. Schmid-Schönbein
Keyword(s):  
Free Radical ◽  
Spontaneously Hypertensive Rats ◽  
Oxygen Free Radical ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Free Radical Production ◽  
Wky Rats ◽  
Arterial Blood ◽  
Radical Production ◽  
Tetrazolium Reduction

One mechanism for organ damage in individuals with arterial hypertension may be due to oxygen free radical production. This study was designed to localize free radicals in a microvascular network of mature spontaneously hypertensive rats (SHRs) and normotensive Wistar-Kyoto (WKY) rats. Because glucocorticoids play a role in pressure elevation of SHRs, we investigated their role in microvascular free radical formation. Oxygen radical production in mesentery was detected by tetranitroblue tetrazolium reduction to formazan aided by digital light-absorption measurements. Formazan deposits were observed in the endothelial cells and lumens of all microvessels and in lymphatic endothelia but were fewer in tissue parenchyma. The formazan distribution in younger (14–16 wk old) WKY rats and SHRs was heterogeneous with low values in capillaries and small arterioles/venules (<30 μm) but enhanced deposits in larger venules. Adrenalectomy served to reduce the formazan density in SHRs to the level of WKY rats, whereas dexamethasone supplementation of the adrenalectomized rats caused elevation in the larger venules of SHRs. In older (40 wk old) SHRs, formazan levels were elevated in all hierarchies of microvessels. After pressure reduction was employed with chronic hydralazine treatment, the formazan deposits were reduced in all locations of the microcirculation in both WKY rats and SHRs. Elevated formazan deposits were also found in lymphatic endothelium. These results suggest that oxygen free radical production is elevated in both high- and low-pressure regions of SHR microcirculation via a process that is controlled by glucocorticoids. Older SHRs have higher formazan levels than younger SHRs in all microvessels. Chronic hydralazine treatment, which serves to reduce arterial blood pressure, attenuates tetranitroblue tetrazolium reduction in WKY rats and SHRs even in venules of the microcirculation, which has no micropressure elevation. Free radical production may be a more global condition in SHRs and may not be limited to arteries and arterioles.

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