scholarly journals Serum Cardiac Troponin T in Patients with Acute Myocardial Infarction. Detection of Coronary Reperfusion and Prediction of Cardiac Function.

1995 ◽  
Vol 36 (3) ◽  
pp. 293-303
Author(s):  
Hiroaki KITAOKA ◽  
Shunichi MIYAZAKI ◽  
Hiroshi NONOGI ◽  
Takashi FURUNO ◽  
Akira ITOH ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Cardiac Function ◽  
Cardiac Troponin ◽  
Troponin T ◽  
Cardiac Troponin T ◽  
Coronary Reperfusion ◽  
Serum Cardiac Troponin

scholarly journals Cardiac Troponin T at 96 Hours After Acute Myocardial Infarction Correlates With Infarct Size and Cardiac Function

2006 ◽  
Vol 48 (11) ◽  
pp. 2192-2194 ◽  
Author(s):  
Henning Steen ◽  
Evangelos Giannitsis ◽  
Simon Futterer ◽  
Constanze Merten ◽  
Claus Juenger ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Cardiac Function ◽  
Infarct Size ◽  
Cardiac Troponin ◽  
Troponin T ◽  
Cardiac Troponin T

scholarly journals 1-h Evaluation for Acute Myocardial Infarction Using the Generation 5 Cardiac Troponin T Assay

2018 ◽  
Vol 72 (21) ◽  
pp. 2677-2679 ◽  
Author(s):  
Richard M. Nowak ◽  
Chaun M. Gandolfo ◽  
Gordon Jacobsen ◽  
Robert H. Christenson ◽  
Michele Moyer ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Cardiac Troponin ◽  
Troponin T ◽  
Cardiac Troponin T

scholarly journals Prospective validation of a 1-hour algorithm to rule-out and rule-in acute myocardial infarction using a high-sensitivity cardiac troponin T assay

2015 ◽  
Vol 187 (8) ◽  
pp. E243-E252 ◽  
Author(s):  
Tobias Reichlin ◽  
Raphael Twerenbold ◽  
Karin Wildi ◽  
Maria Rubini Gimenez ◽  
Nathalie Bergsma ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Cardiac Troponin ◽  
Troponin T ◽  
High Sensitivity ◽  
Cardiac Troponin T ◽  
Rule Out

scholarly journals Identification and Characterization of Cardiac Troponin T Fragments in Serum of Patients Suffering from Acute Myocardial Infarction

2017 ◽  
Vol 63 (2) ◽  
pp. 563-572 ◽  
Author(s):  
Alexander S Streng ◽  
Douwe de Boer ◽  
William P T M van Doorn ◽  
Freek G Bouwman ◽  
Edwin C M Mariman ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Mass Spectrometry ◽  
Acute Myocardial Infarction ◽  
Western Blotting ◽  
Cardiac Troponin ◽  
Epitope Mapping ◽  
Troponin T ◽  
Amino Acid Sequences ◽  
Cardiac Troponin T ◽  
Selected Ion Monitoring

AbstractBACKGROUNDCardiac troponin T (cTnT) is the preferred biomarker for the diagnosis of acute myocardial infarction (AMI). It has been suggested that cTnT is present predominantly in fragmented forms in human serum following AMI. In this study, we have used a targeted mass spectrometry assay and epitope mapping using Western blotting to confirm this hypothesis.METHODScTnT was captured from the serum of 12 patients diagnosed with AMI using an immunoprecipitation technique employing the M11.7 catcher antibody and fractionated with SDS-PAGE. Coomassie-stained bands of 4 patients at 37, 29, and 16 kDa were excised from the gel, digested with trypsin, and analyzed on a Q Exactive instrument set on targeted Selected Ion Monitoring mode with data-dependent tandem mass spectrometry (MS/MS) for identification. Western blotting employing 3 different antibodies was used for epitope mapping.RESULTSTen cTnT peptides of interest were targeted. By using MS/MS, all of these peptides were identified in the 37-kDa, intact, cTnT band. In the 29- and 16-kDa fragment bands, 8 and 4 cTnT-specific peptides were identified, respectively. Some of these peptides were “semitryptic,” meaning that their C-termini were not formed by trypsin cleavage. The C-termini of these semitryptic peptides represent the C-terminal end of the cTnT molecules present in these bands. These results were confirmed independently by epitope mapping.CONCLUSIONSUsing LC-MS, we have succeeded in positively identifying the 29- and 16-kDa fragment bands as cTnT-derived products. The amino acid sequences of the 29- and 16-kDa fragments are Ser79-Trp297 and Ser79-Gln199, respectively.

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