scholarly journals Edematous Arterial Reaction by Adrenaline and Cholesterol and Its Prevention by MAO Inhibitor Observed by Electron Microscopic Technique

1962 ◽  
Vol 3 (6) ◽  
pp. 581-601 ◽  
Author(s):  
Takio SHIMAMOTO ◽  
Toshiaki SUNAGA
Keyword(s):  
Microscopic Technique ◽  
Electron Microscopic ◽  
Mao Inhibitor ◽  
Electron Microscopic Technique

Electron Microscopic Study of the Locus of Latex Reaction

1955 ◽  
Vol 28 (2) ◽  
pp. 623-627
Author(s):  
Edward Crampsey ◽  
Manfred Gordon ◽  
John W. Sharpe
Keyword(s):  
Hydrochloric Acid ◽  
Diffusion Processes ◽  
Polymer Particle ◽  
Microscopic Technique ◽  
Emulsion System ◽  
Electron Microscopic ◽  
Quantitative Evidence ◽  
Aqueous Acid ◽  
Electron Microscopic Technique ◽  
The Individual

Abstract The outstanding example of the study of reaction loci in an emulsion system is Harkins's work in the field of emulsion polymerization. A simpler case presents itself for study when chemical reactions are carried out with preformed polymer emulsions (latexes), as in the hydrochlorination of polyisoprene latex. Here the reaction consists in the progressive conversion of the individual unsaturated isoprene units to their hydrochlorinated form: (see PDF for diagram) the polymer particles preserving their identity throughout the reaction. The hydrochloric acid arrives at the site of reaction by a series of diffusion processes from the vapor phase and through the continuous aqueous phase. In principle there are still two loci at which the isoprene units may react with this reagent: in the bulk of the particle and at (or near) the interphase between the aqueous acid and the polymer particle. We were led by kinetic experiments to conclude that the two loci do react at different rates and by different mechanisms, and it is the purpose of this report to provide quantitative evidence for this conclusion. At the same time we wish to put on record some observations connected with the electron microscopic technique used in the investigation.

An Embedding Mixture for Electron Microscopic Technique Composed Solely of Maraglas, Araldite and Catalyst

1968 ◽  
Vol 26 ◽  
pp. 242-243
Author(s):  
Dale E. McClendon ◽  
Bernard L. Soloff
Keyword(s):  
Propylene Oxide ◽  
Microscopic Technique ◽  
Electron Microscopic ◽  
Thin Sections ◽  
Low Intensity ◽  
Electron Microscopic Technique ◽  
Hard Block ◽  
The Stability

We have found the following embedding mixture to be useful: Maraglas 655 - 70 ml., Araldite 502 - 30 ml., benzyldimethylamine - 3 ml. The tissue blocks are processed routinely through a 1:3 mixture of propylene oxide: complete embedding mixture. After overnight soaking in this mixture, the tissue blocks are allowed to sink for two hours in capsules loaded with fresh embedding mixture. The capsules are then processed four hours in a 37 - 40° C. oven, followed by three days in a 65° C. oven. This treatment yields a hard block that may be sectioned immediately upon cooling. The thin sections obtained will accept readily any of the staining solutions now available.Elimination of the anhydride hardener used normally in formulations of this sort does not affect the stability of thin sections under a 50 or 75 KV beam (figures 1 and 3), and sections supported on naked grids will withstand a 100 KV beam (figure 2) if carefully irradiated at low intensity before exposure to cross-over conditions.

An electron microscopic technique for in situ observation of hematopoietic colonies

Micron (1969) ◽  
1979 ◽  
Vol 10 (3) ◽  
pp. 163-164
Author(s):  
Mark J. Ottenbreit ◽  
Robert O. Bollinger ◽  
Fred A. Uchwat ◽  
Susumu Inoue
Keyword(s):  
In Situ Observation ◽  
Microscopic Technique ◽  
Electron Microscopic ◽  
Electron Microscopic Technique
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