scholarly journals Histological studies on staining characteristic differences in arterial elastic fibers

1980 ◽  
Vol 21 (4) ◽  
pp. 596-596
Author(s):  
Shiko Chichibu ◽  
Hiroyuki Ito ◽  
Kozo Okamoto
Keyword(s):  
Elastic Fibers ◽  
Histological Studies ◽  
Staining Characteristic

Ultrastructure and Staining of Developing Elastic Tissue

1971 ◽  
Vol 29 ◽  
pp. 244-245
Author(s):  
E. N. Albert
Keyword(s):  
Fine Structure ◽  
Phosphotungstic Acid ◽  
Staining Method ◽  
Rat Aorta ◽  
Uranyl Acetate ◽  
The Other ◽  
Elastic Fibers ◽  
Elastic Tissue ◽  
Lead Citrate ◽  
New Born

Silver tetraphenylporphine sulfonate (Ag-TPPS) was synthesized in this laboratory and used as an electron dense stain for elastic tissue (Fig 1). The procedures for the synthesis of tetraphenylporphine sulfonate and the staining method for mature elastic tissue have been described previously.The fine structure of developing elastic tissue was observed in fetal and new born rat aorta using tetraphenylporphine sulfonate, phosphotungstic acid, uranyl acetate and lead citrate. The newly forming elastica consisted of two morphologically distinct components. These were a central amorphous and a peripheral fibrous. The ratio of the central amorphous and the peripheral fibrillar portion changed in favor of the former with increasing age.It was also observed that the staining properties of the two components were entirely different. The peripheral fibrous component stained with uranyl acetate and/or lead citrate while the central amorphous portion demonstrated no affinity for these stains. On the other hand, the central amorphous portion of developing elastic fibers stained vigorously with silver tetraphenylporphine sulfonate, while the fibrillar part did not (compare figs 2, 3, 4). Based upon the above observations it is proposed that developing elastica consists of two components that are morphologically and chemically different.

CRYPTORCHIDISM: CLINICAL, HORMONAL AND HISTOLOGICAL STUDIES

1974 ◽  
Vol 77 (1_Suppl) ◽  
pp. S51 ◽  
Author(s):  
A. Attanasio ◽  
K. Jendricke ◽  
J. R. Bierich ◽  
D. Gupta ◽  
G. Bulle ◽  
...  
Keyword(s):  
Histological Studies

Clinical and histopathological studies on basalioma - Elastic fibers in basalioma.

1985 ◽  
Vol 47 (5) ◽  
pp. 858-863 ◽  
Author(s):  
Akira KAWADA ◽  
Seiji KONDO ◽  
Akira MAMADA
Keyword(s):  
Elastic Fibers ◽  
Histopathological Studies

Morpho-histological studies of three Romanian Solidago species

2020 ◽  
Vol 53 (1) ◽  
Author(s):  
Luciana Dobjanschi ◽  
Eva Brigitta Patay ◽  
Luminiţa Fritea ◽  
Mircea Tămaş ◽  
Alexandru Jurca ◽  
...  
Keyword(s):  
Histological Studies

Histological Studies on Experimental Viral Arthritis

1962 ◽  
Vol 8 (1) ◽  
pp. 265-280 ◽  
Author(s):  
S. Taniguchi ◽  
K. Shichikawa
Keyword(s):  
Histological Studies

Histological studies on development of the digestive system of Scortum barcoo

2010 ◽  
Vol 34 (2) ◽  
pp. 264-270
Author(s):  
Yu-jiang LUO ◽  
Kun-ci CHEN ◽  
Xin-ping ZHU ◽  
De-bo PAN ◽  
Ou-qin CHANG ◽  
...  
Keyword(s):  
Digestive System ◽  
Histological Studies

THE EFFECT OF OINTMENTS WITH ANTIOXIDANT ACTIVITY ON THE MORPHOLOGICAL STRUCTURES OF SKIN OF GUINEA PIGS EXPOSED TO LOCAL ULTRAVIOLET IRRADIATION

2019 ◽  
pp. 64-72
Author(s):  
T. V. Zvyagintseva ◽  
S. I. Myronchenko ◽  
N. I. Kytsyuk ◽  
O. V. Naumova
Keyword(s):  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Ultraviolet Irradiation ◽  
Guinea Pigs ◽  
Main Group ◽  
The State ◽  
Elastic Fibers ◽  
Control Group ◽  
General Tendency ◽  
Skin Reactions

Considering the particular danger of remote skin reactions to ultraviolet irradiation (UVI), it is advisable to use ointments with antioxidant activity to reduce its negative effect on the skin. The rationale for the choice of ointments with antioxidant activity was the fact that they reduce the damaging effect of ultraviolet radiation in the erythemal and early post-erythemal period. The presence of a regular connection between the development of the early and late periods has given reason to assume the protective effect of ointments on the remote skin reactions. Objective: to study the effect of thiotriazoline ointment and thiotriazoline ointment with silver nanoparticles on the state of the morphological structures of the skin of guinea pigs after local UVI. Material and methods of research. The study involved 132 albino guinea pigs weighing 400-500 g, divided into 4 groups: 1 - intact, 2 - control (guinea pigs subjected to local UVI), 3 and 4 main ones. The third main group included guinea pigs that after UVI were administered thiotriazoline ointment in the treatment and prophylactic regime, the fourth main group included guinea pigs that after UVI were administered thiotriazoline ointment with silver nanoparticles in the same mode as Group 3. Ointments were applied 1 hour before irradiation and daily until erythema disappeared. Ultraviolet erythema was caused by irradiation in 1 minimum erythemal dose. After 2, 4 hours, on the 3rd, 8th, 15th, 21st, 28th day, the fragments of irradiated skin were investigated using histochemical and morphometric methods (fibroblast density and epidermis thickness). Results. Morphological changes in the skin after applying ointments with antioxidant activity were unidirectional. It was revealed that in the early periods after irradiation, thiotrazoline ointment and thiotrazoline ointment with silver nanoparticles do not affect changes in the thickness of the epidermis, but statistically significantly reduce the density of fibroblasts in the dermis on the 3rd day of the experiment compared to the control group. In the later periods, under the influence of thiotriazoline ointment, a gradual decrease in the thickness of the epidermis, which reached the norm by the end of the experiment, was observed. On the 8th day, the maximum density of fibroblasts was recorded, in the subsequent periods of the experiment, the index gradually decreased, which was accompanied by collagenization of the papillary layer in the loci of damage to collagen and elastic fibers detected in 50% of cases. In later times, under the influence of thiotriazoline ointment with silver nanoparticles, the processes of restoring the morphological structures of the skin occurred faster. In parallel with the decrease in the density of fibroblasts in the loci of the previous damage to the collagen and elastic fibers of the papillary layer, thickening of collagen fibers was observed, replacing them with segments of destruction of elastic fibers. In this group, at the end of the experiment, the collagenization locus was small, single, occurring in 16.7% of cases. Conclusions Ointments with antioxidant activity exert a positive effect on the state of morphological structures of the skin, damaged as a result of local UVI, in erythemal and post-erythemic periods. In the early periods after the local UVI, there was a general tendency for the effect of both ointments, as they reduced the density of fibroblasts on the 3rd day, but did not result in complete normalization. In the late period after local UVI , under the influence of thiotriazoline ointment and thiotriazoline ointment with silver nanoparticles, thickness of the epidermis (by 21st and 15th day, respectively) and density of fibroblasts (by the 28th day) decreased to normal while without treatment both indicators exceeded the norm by several times for 28 days of the experiment.

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