scholarly journals DNA Variability and Divergence at the Notch Locus in Drosophila melanogaster and D. simulans: A Case of Accelerated Synonymous Site Divergence

Genetics ◽  
2004 ◽  
Vol 167 (1) ◽  
pp. 171-185 ◽  
Author(s):  
Vanessa Bauer DuMont ◽  
Justin C. Fay ◽  
Peter P. Calabrese ◽  
Charles F. Aquadro
Keyword(s):  
Drosophila Melanogaster ◽  
Synonymous Site ◽  
Notch Locus

scholarly journals NEGATIVE COMPLEMENTATION AT THE NOTCH LOCUS OF DROSOPHILA MELANOGASTER

Genetics ◽  
1975 ◽  
Vol 81 (1) ◽  
pp. 99-120 ◽  
Author(s):  
Geoffrey G Foster
Keyword(s):  
Drosophila Melanogaster ◽  
Notch Locus

ABSTRACT Four Abruptex alleles (AxE1, AxE  2, Ax9  B  2, and Ax16172) have been mapped within the Notch locus. Based on their visible phenotypes and their interactions with one another and with N mutations, the Ax alleles can be divided into two groups. Heterozygous combinations of members of the same group are intermediate in phenotype compared to the respective homozygotes, whereas heterozygotes of Ax alleles from different groups exhibit negative heterosis, being much less viable and more extremely mutant than either homozygote. It is suggested that the Notch locus is a multi-functional regulator ("integrator") gene, whose product possesses both "repressor" and "activator" functions for the processes it regulates.

The molecular genetics of the Notch locus in Drosophila melanogaster

1985 ◽  
Vol 107 (2) ◽  
pp. 503-519 ◽  
Author(s):  
Brian G. Grimwade ◽  
Marc A.T. Muskavitch ◽  
William J. Welshons ◽  
Barry Yedvobnick ◽  
Spyros Artavanis-Tsakonas
Keyword(s):  
Drosophila Melanogaster ◽  
Molecular Genetics ◽  
Notch Locus

scholarly journals Phenotypic and molecular analysis of the facets, a group of intronic mutations at the Notch locus of Drosophila melanogaster which affect postembryonic development.

Genetics ◽  
1989 ◽  
Vol 122 (2) ◽  
pp. 417-428 ◽  
Author(s):  
K Markopoulou ◽  
W J Welshons ◽  
S Artavanis-Tsakonas
Keyword(s):  
Drosophila Melanogaster ◽  
Transposable Element ◽  
Larval Instar ◽  
Postembryonic Development ◽  
Mutant Phenotype ◽  
Transcriptional Analysis ◽  
Mutational Event ◽  
Recessive Mutations ◽  
Notch Locus ◽  
Element Display

Abstract The function of the Notch locus of Drosophila melanogaster is essential for normal development both during embryogenesis and during postembryonic stages. In the embryo its function is necessary for the correct segregation of neural from epidermal lineages. During postembryonic stages Notch exhibits pleiotropic effects that are both tissue- and stage-specific. Here, we examine a group of six recessive mutations, the facets (fa, fa3, fag, fag-2, fafx and fasw), which affect eye morphology and have been previously shown to be associated with the insertion of transposable elements in an intronic region of Notch. The analysis of revertants has shown that the mutant phenotype depends on the presence of the transposable element and that the disruption of the wild-type sequence organization per se is not its cause. Four of these alleles, even though they are associated with the insertion of the same transposable element, display considerably different phenotypes. Therefore, no simple correlation exists between the mutant phenotype and the type of inserted element. A comparison of the tissue localization of the Notch and the transposable element transcripts revealed that in the third larval instar the elements are transcribed in both orientations in tissues in which Notch is also transcriptionally active. The complexity of the defects associated with the facet alleles, as well as the findings of the transcriptional analysis, indicate that a mutational mechanism based solely on transcriptional interference is not sufficient to explain the nature of the mutational event. It is likely that in these mutations alterations, in the temporal and/or spatial context caused by transcriptional and perhaps posttranscriptional interference mechanisms by the inserted elements, may be responsible for the mutant phenotype.

scholarly journals A tripartite interaction among alleles of Notch, Delta, and Enhancer of split during imaginal development of Drosophila melanogaster.

Genetics ◽  
1989 ◽  
Vol 122 (2) ◽  
pp. 429-438
Author(s):  
S B Shepard ◽  
S A Broverman ◽  
M A Muskavitch
Keyword(s):  
Drosophila Melanogaster ◽  
Compound Eye ◽  
Recessive Allele ◽  
Gain Of Function ◽  
Enhancing Effect ◽  
Notch Locus ◽  
Tripartite Interaction ◽  
Extragenic Suppressors ◽  
Enhancer Of Split

Abstract A dramatic example of a phenotypic interaction that involves neurogenic loci during Drosophila imaginal development is the synergistic impact of split (spl), a recessive allele of the Notch locus, and E(spl)D, a dominant gain-of-function allele of the Enhancer of split locus, on morphogenesis of the compound eye. Screens for mutations that relieve the enhancing effect of E(spl)D on spl have yielded three classes of mutations: intragenic revertants of the E(spl)D allele, extragenic suppressors that are allelic to the neurogenic gene Delta (Dl) and unlinked extragenic modifiers. Analysis of the suppression of the spl-E(spl)D interaction by various Dl alleles indicates that this modification is sensitive to the dosage of the Dl locus. This tripartite interaction illustrates the combinatorial action of N, Dl and E(spl) during imaginal development.

scholarly journals deltex, a locus interacting with the neurogenic genes, Notch, Delta and mastermind in Drosophila melanogaster.

Genetics ◽  
1990 ◽  
Vol 126 (3) ◽  
pp. 665-677 ◽  
Author(s):  
T Xu ◽  
S Artavanis-Tsakonas
Keyword(s):  
Drosophila Melanogaster ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Transmembrane Protein ◽  
Wild Type ◽  
Linked Gene ◽  
Neurogenic Genes ◽  
Allele Specific ◽  
Epidermal Growth ◽  
Notch Locus

Abstract The Notch locus of Drosophila melanogaster, which codes for a transmembrane protein sharing homology with the mammalian epidermal growth factor, is one of a small number of zygotically acting genes, the so called neurogenic loci, which are necessary for the correct segregation of neural from epidermal lineages during embryogenesis. In an attempt to identify genes whose products may interact with that of Notch, we designed a genetic screen aimed at identifying suppressors of certain Notch mutations which are known to affect the extracellular epidermal growth factor homologous domain of Notch. Mutations in two neurogenic loci were identified as suppressors: Delta, whose product was recently shown to interact with Notch and mastermind. In addition, a third, X-linked gene was shown capable of acting as a suppressor. We show that this gene is the deltex locus, characterize the phenotype of deltex mutations, and demonstrate both a maternal and zygotic action of the locus. All deltex alleles behave as recessive viables affecting wing, ocellar and eye morphology. There are allele specific interactions between deltex and various Notch alleles; for example, deltex mutants with a reduced dosage of wild-type Notch die as pupae. deltex also interacts with Delta and mastermind in a fashion that is formally analogous to its interaction with Notch. These results emphasize the special relationship between Notch, Delta and mastermind suggested by previous work and indicate that deltex is likely to play an important role in the same genetic circuitry within which these three neurogenic loci operate.

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