scholarly journals A Genetic Screen for Neurite Outgrowth Mutants in Caenorhabditis elegans Reveals a New Function for the F-box Ubiquitin Ligase Component LIN-23

Genetics ◽  
2004 ◽  
Vol 166 (3) ◽  
pp. 1253-1267 ◽  
Author(s):  
Nehal Mehta ◽  
Paula M. Loria ◽  
Oliver Hobert
Keyword(s):  
Caenorhabditis Elegans ◽  
Neurite Outgrowth ◽  
Ubiquitin Ligase ◽  
Genetic Screen

scholarly journals The UBR-1 ubiquitin ligase regulates glutamate metabolism to generate coordinated motor pattern in Caenorhabditis elegans

PLoS Genetics ◽  
2018 ◽  
Vol 14 (4) ◽  
pp. e1007303
Author(s):  
Jyothsna Chitturi ◽  
Wesley Hung ◽  
Anas M. Abdel Rahman ◽  
Min Wu ◽  
Maria A. Lim ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Ubiquitin Ligase ◽  
Motor Pattern ◽  
Glutamate Metabolism

scholarly journals The Caenorhabditis elegans Replication Licensing Factor CDT-1 Is Targeted for Degradation by the CUL-4/DDB-1 Complex

2006 ◽  
Vol 27 (4) ◽  
pp. 1394-1406 ◽  
Author(s):  
Youngjo Kim ◽  
Edward T. Kipreos
Keyword(s):  
Caenorhabditis Elegans ◽  
Ubiquitin Ligase ◽  
S Phase ◽  
Null Mutant ◽  
Wild Type ◽  
C Elegans ◽  
Licensing Factor ◽  
Ubiquitin Ligase E3 ◽  
Blast Cells ◽  
Temporal Restriction

ABSTRACT The replication of genomic DNA is strictly regulated to occur only once per cell cycle. This regulation centers on the temporal restriction of replication licensing factor activity. Two distinct ubiquitin ligase (E3) complexes, CUL4/DDB1 and SCFSkp2, have been reported to target the replication licensing factor Cdt1 for ubiquitin-mediated proteolysis. However, it is unclear to what extent these two distinct Cdt1 degradation pathways are conserved. Here, we show that Caenorhabditis elegans DDB-1 is required for the degradation of CDT-1 during S phase. DDB-1 interacts specifically with CUL-4 but not with other C. elegans cullins. A ddb-1 null mutant exhibits extensive DNA rereplication in postembryonic BLAST cells, similar to what is observed in cul-4(RNAi) larvae. DDB-1 physically associates with CDT-1, suggesting that CDT-1 is a direct substrate of the CUL-4/DDB-1 E3 complex. In contrast, a deletion mutant of the C. elegans Skp2 ortholog, skpt-1, appears overtly wild type with the exception of an impenetrant gonad migration defect. There is no appreciable role for SKPT-1 in the degradation of CDT-1 during S phase, even in a sensitized ddb-1 mutant background. We propose that the CUL-4/DDB-1 ubiquitin ligase is the principal E3 for regulating the extent of DNA replication in C. elegans.

scholarly journals COMP-1 promotes competitive advantage of nematode sperm

eLife ◽  
2015 ◽  
Vol 4 ◽  
Author(s):  
Jody M Hansen ◽  
Daniela R Chavez ◽  
Gillian M Stanfield
Keyword(s):  
Sexual Selection ◽  
Caenorhabditis Elegans ◽  
Competitive Advantage ◽  
Sexual Reproduction ◽  
Functional Traits ◽  
Reproductive Tract ◽  
Genetic Screen ◽  
Dependent Manner ◽  
Cellular Mechanisms ◽  
Forward Genetic Screen

Competition among sperm to fertilize oocytes is a ubiquitous feature of sexual reproduction as well as a profoundly important aspect of sexual selection. However, little is known about the cellular mechanisms sperm use to gain competitive advantage or how these mechanisms are regulated genetically. In this study, we utilize a forward genetic screen in Caenorhabditis elegans to identify a gene, comp-1, whose function is specifically required in competitive contexts. We show that comp-1 functions in sperm to modulate their migration through and localization within the reproductive tract, thereby promoting their access to oocytes. Contrary to previously described models, comp-1 mutant sperm show no defects in size or velocity, thereby defining a novel pathway for preferential usage. Our results indicate not only that sperm functional traits can influence the outcome of sperm competition, but also that these traits can be modulated in a context-dependent manner depending on the presence of competing sperm.

Viable maternal-effect mutations that affect the development of the nematode Caenorhabditis elegans.

Genetics ◽  
1995 ◽  
Vol 141 (4) ◽  
pp. 1351-1364 ◽  
Author(s):  
S Hekimi ◽  
P Boutis ◽  
B Lakowski
Keyword(s):  
Quantitative Analysis ◽  
Caenorhabditis Elegans ◽  
Maternal Effect ◽  
Genetic Screen ◽  
Phenotypic Characterization ◽  
Nematode Caenorhabditis Elegans ◽  
Critical Function ◽  
Complementation Groups

Abstract We carried out a genetic screen for viable maternal-effect mutants to identify genes with a critical function relatively early in development. This type of mutation would not have been identified readily in previous screens for viable mutants and therefore could define previously unidentified genes. We screened 30,000 genomes and identified 41 mutations falling into 24 complementation groups. We genetically mapped these 24 loci; only two of them appear to correspond to previously identified genes. We present a partial phenotypic characterization of the mutants and a quantitative analysis of the degree to which they can be maternally or zygotically rescued.

scholarly journals Generation of an external guide sequence library for a reverse genetic screen in Caenorhabditis elegans

2009 ◽  
Vol 9 (1) ◽  
pp. 47 ◽  
Author(s):  
Qitao Yan ◽  
Rui Zhao ◽  
Wenlin Zheng ◽  
Changxin Yin ◽  
Bao Zhang ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Genetic Screen ◽  
Reverse Genetic ◽  
Guide Sequence ◽  
Reverse Genetic Screen ◽  
External Guide Sequence

scholarly journals A Genetic Screen for Mutants with Supersized Lipid Droplets in Caenorhabditis elegans

2016 ◽  
Vol 6 (8) ◽  
pp. 2407-2419 ◽  
Author(s):  
Shiwei Li ◽  
Shibin Xu ◽  
Yanli Ma ◽  
Shuang Wu ◽  
Yu Feng ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Lipid Droplets ◽  
Genetic Screen

scholarly journals A Wnt-Frz/Ror-Dsh Pathway Regulates Neurite Outgrowth in Caenorhabditis elegans

PLoS Genetics ◽  
2010 ◽  
Vol 6 (8) ◽  
pp. e1001056 ◽  
Author(s):  
Song Song ◽  
Bo Zhang ◽  
Hui Sun ◽  
Xia Li ◽  
Yanhui Xiang ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Neurite Outgrowth
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