scholarly journals Recombination Within a Nucleotide-Binding-Site/Leucine-Rich-Repeat Gene Cluster Produces New Variants Conditioning Resistance to Soybean Mosaic Virus in Soybeans

Genetics ◽  
2004 ◽  
Vol 166 (1) ◽  
pp. 493-503 ◽  
Author(s):  
A. J. Hayes ◽  
S. C. Jeong ◽  
M. A. Gore ◽  
Y. G. Yu ◽  
G. R. Buss ◽  
...  
Keyword(s):  
Binding Site ◽  
Mosaic Virus ◽  
Gene Cluster ◽  
Soybean Mosaic Virus ◽  
Nucleotide Binding ◽  
Nucleotide Binding Site ◽  
Leucine Rich Repeat

Tracing the origin and evolutionary history of plant nucleotide-binding site-leucine-rich repeat (NBS-LRR) genes

2011 ◽  
Vol 193 (4) ◽  
pp. 1049-1063 ◽  
Author(s):  
Jia-Xing Yue ◽  
Blake C. Meyers ◽  
Jian-Qun Chen ◽  
Dacheng Tian ◽  
Sihai Yang
Keyword(s):  
Binding Site ◽  
Evolutionary History ◽  
Nucleotide Binding ◽  
Nucleotide Binding Site ◽  
Leucine Rich Repeat ◽  
History Of

A superfamily of disease resistance gene analogs is located on all homoeologous chromosome groups of wheat (Triticum aestivum)

Genome ◽  
1998 ◽  
Vol 41 (6) ◽  
pp. 782-788 ◽  
Author(s):  
W Spielmeyer ◽  
M Robertson ◽  
N Collins ◽  
D Leister ◽  
P Schulze-Lefert ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Binding Site ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Nucleotide Binding ◽  
Nucleotide Binding Site ◽  
Resistance Gene Analogs ◽  
Leucine Rich Repeat ◽  
Homoeologous Chromosome ◽  
Entry Points

In this study, resistance gene analogs (RGAs) which were isolated from monocot crop species (wheat, barley, maize and rice) and contained conserved sequence motifs found within the nucleotide binding site - leucine rich repeat (NBS-LRR) class of resistance genes, were used to assess their distribution in the wheat genome. The RGAs showed 30-70% amino acid identity to a previously isolated monocot NBS-LRR sequence from the Cre3 locus for cereal cyst nematode (CCN) resistance in wheat. We used the RGAs as probes to identify and map loci in wheat using recombinant inbred lines of an international Triticeae mapping family. RGA loci mapped across all seven homoeologous chromosome groups of wheat. This study demonstrated that the RGA mapping approach provides potential entry points toward identifying resistance gene candidates in wheat.Key words: wheat, disease resistance genes, nucleotide binding site, leucine rich repeat, resistance gene analogs.

scholarly journals Long-Term Evolution of Nucleotide-Binding Site-Leucine-Rich Repeat Genes: Understanding Gained from and beyond the Legume Family

2014 ◽  
Vol 166 (1) ◽  
pp. 217-234 ◽  
Author(s):  
Zhu-Qing Shao ◽  
Yan-Mei Zhang ◽  
Yue-Yu Hang ◽  
Jia-Yu Xue ◽  
Guang-Can Zhou ◽  
...  
Keyword(s):  
Binding Site ◽  
Nucleotide Binding ◽  
Long Term Evolution ◽  
Nucleotide Binding Site ◽  
Leucine Rich Repeat ◽  
Term Evolution

scholarly journals Dm3 Is One Member of a Large Constitutively Expressed Family of Nucleotide Binding Site—Leucine-Rich Repeat Encoding Genes

2002 ◽  
Vol 15 (3) ◽  
pp. 251-261 ◽  
Author(s):  
Katherine A. Shen ◽  
Doris B. Chin ◽  
Rosa Arroyo-Garcia ◽  
Oswaldo E. Ochoa ◽  
Dean O. Lavelle ◽  
...  
Keyword(s):  
Binding Site ◽  
Downy Mildew ◽  
Point Mutations ◽  
Nucleotide Binding ◽  
Nucleotide Binding Site ◽  
Leucine Rich Repeat ◽  
Intron Splice ◽  
Major Cluster ◽  
Wide Range ◽  
Encoding Genes

The major cluster of resistance genes in lettuce cv. Diana contains approximately 32 nucleotide binding site—leucine-rich repeat encoding genes. Previous molecular dissection of this complex region had identified a large gene, RGC2B, as a candidate for encoding the downy mildew resistance gene, Dm3. This article describes genetic and transgenic complementation data that demonstrated RGC2B is necessary and sufficient to confer resistance with Dm3 specificity. Ethylmethanesulphonate was used to induce mutations to downy mildew susceptibility in cv. Diana (Dm1, Dm3, Dm7, and Dm8). Nineteen families were identified with a complete loss of resistance in one of the four resistance specificities. Sequencing revealed a variety of point mutations in RGC2B in the six dm3 mutants. Losses of resistance were due to single changes in amino acid sequence or a change in an intron splice site. These mutations did not cluster in any particular region of RGC2B. A full-length ge-nomic copy of RGC2B was isolated from a lambdaphage library and introduced into two genotypes of lettuce. Trans-genics expressing RGC2B exhibited resistance to all isolates expressing Avr3 from a wide range of geographical origins. In a wildtype Dm3-expressing genotype, many of the RGC2 family members are expressed at low levels throughout the plant.

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