scholarly journals Targeted Heritable Mutation and Gene Conversion by Cas9-CRISPR in Caenorhabditis elegans

Genetics ◽  
2013 ◽  
Vol 195 (3) ◽  
pp. 1173-1176 ◽  
Author(s):  
Iskra Katic ◽  
Helge Großhans
Keyword(s):  
Caenorhabditis Elegans ◽  
Gene Conversion ◽  
Heritable Mutation

scholarly journals GENETIC ORGANIZATION IN CAENORHABDITIS ELEGANS: FINE-STRUCTURE ANALYSIS OF THE unc-22 GENE

Genetics ◽  
1979 ◽  
Vol 91 (1) ◽  
pp. 95-103 ◽  
Author(s):  
D G Moerman ◽  
D L Baillie
Keyword(s):  
Drosophila Melanogaster ◽  
Fine Structure ◽  
Caenorhabditis Elegans ◽  
Gene Conversion ◽  
Structure Analysis ◽  
Intragenic Recombination ◽  
Preliminary Estimate ◽  
Recessive Mutations ◽  
Fine Structure Analysis ◽  
New Alleles

ABSTRACT Fine-structure analysis of the unc-22 gene of Caenorhabditis elegarns has revealed a number of sites that are separable by recombination. Eight new ethyl methanesulfonate-induced recessive mutations of the unc-22 gene have been isolated. Using these new alleles, as well as e66, a number of separable sites have been identified and positioned relative to one another. The map distances obtained are found to be comparable to those associated with intragenic recombination in Drosophila melanogaster, indicating that genetic finestructure analysis is feasible in Caenorhabditis elegans. Evidence of possible gene conversion is presented. A preliminary estimate of the unc-22 gene size is 2.4 × 10-2 map units.

scholarly journals Increased intragenic recombination and non-disjunction in the Rec-1 strain of Caenorhabditis elegans

1988 ◽  
Vol 51 (2) ◽  
pp. 89-93 ◽  
Author(s):  
B. Rattray ◽  
A. M. Rose
Keyword(s):  
Caenorhabditis Elegans ◽  
Gene Conversion ◽  
Recombination Frequency ◽  
Spontaneous Mutation ◽  
Selective Advantage ◽  
Dna Lesions ◽  
Intragenic Recombination ◽  
Crossing Over ◽  
Spontaneous Mutation Rate ◽  
Independent Events

SummaryThe Rec-1 strain of Caenorhabditis elegans increases recombination frequency three-fold. In this paper, we have investigated the effect of Rec-1 on the intragenic recombination phenomena of crossing-over and gene conversion. These events were increased two- to three-fold as was X-chromosome non-disjunction. All of the recovered recombinants were independent events, indicating that Rec-1 does not act pre-meiotically. The pattern of recombination in the Rec-1 strain resembles a meiotic pattern more than a radiation expansion. We conclude from this result that the Rec-1 enhancement of recombination is not the result of an increased number of DNA lesions randomly distributed along the chromosome. The increased recombination frequency of Rec-1 was not accompanied by any detrimental effects on growth, progeny number or spontaneous mutation rate. In this regard, the results may have implications for models which propose either selective advantage or disadvantage accompanying increased recombination.

scholarly journals Targeted gene alteration in Caenorhabditis elegans by gene conversion

2004 ◽  
Vol 36 (11) ◽  
pp. 1231-1237 ◽  
Author(s):  
Peter L Barrett ◽  
John T Fleming ◽  
Verena Göbel
Keyword(s):  
Caenorhabditis Elegans ◽  
Gene Conversion ◽  
Gene Alteration

scholarly journals Germinal Excisions of the Maize Transposon Activator Do Not Stimulate Meiotic Recombination or Homology-Dependent Repair at the bz Locus

Genetics ◽  
1997 ◽  
Vol 147 (4) ◽  
pp. 1923-1932
Author(s):  
Hugo K Dooner ◽  
Isabel M Martínez-Férez
Keyword(s):  
Caenorhabditis Elegans ◽  
Gene Conversion ◽  
Meiotic Recombination ◽  
Homologous Chromosome ◽  
Tandem Duplication ◽  
Insertion Site ◽  
Double Strand Breaks ◽  
Homologous Sequence ◽  
Strand Breaks ◽  
Ac Transposition

Abstract Double-strand breaks have been implicated both in the initiation of meiotic recombination in yeast and as intermediates in the transposition process of nonreplicative transposons. Some transposons of this class, notably P of Drosophila and Tc1 of Caenorhabditis elegans, promote a form of homology-dependent premeiotic gene conversion upon excision. In this work, we have looked for evidence of an interaction between Ac transposition and meiotic recombination at the bz locus in maize. We find that the frequency of meiotic recombination between homologues is not enhanced by the presence of Ac in one of the bz heteroalleles and, conversely, that the presence of a homologous sequence in either trans (homologous chromosome) or cis (tandem duplication) does not promote conversion of the Ac insertion site. However, a tandem duplication of the bz locus may be destabilized by the insertion of Ac. We discuss possible reasons for the lack of interaction between Ac excision and homologous meiotic recombination in maize.

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