scholarly journals Genome Engineering With Zinc-Finger Nucleases

Genetics ◽  
2011 ◽  
Vol 188 (4) ◽  
pp. 773-782 ◽  
Author(s):  
Dana Carroll
Keyword(s):  
Zinc Finger ◽  
Genome Engineering ◽  
Zinc Finger Nucleases

scholarly journals Targeted genome engineering via zinc finger nucleases

2010 ◽  
Vol 5 (1) ◽  
pp. 9-17 ◽  
Author(s):  
Seokjoong Kim ◽  
Jin-Soo Kim
Keyword(s):  
Zinc Finger ◽  
Genome Engineering ◽  
Zinc Finger Nucleases

scholarly journals Targeted genome editing by lentiviral protein transduction of zinc-finger and TAL-effector nucleases

eLife ◽  
2014 ◽  
Vol 3 ◽  
Author(s):  
Yujia Cai ◽  
Rasmus O Bak ◽  
Jacob Giehm Mikkelsen
Keyword(s):  
Zinc Finger ◽  
Genome Engineering ◽  
Effective Means ◽  
Zinc Finger Nucleases ◽  
Protein Transduction ◽  
Gene Correction ◽  
Transcription Activator ◽  
Homology Directed Repair ◽  
Target Locus ◽  
Programmable Nucleases

Future therapeutic use of engineered site-directed nucleases, like zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs), relies on safe and effective means of delivering nucleases to cells. In this study, we adapt lentiviral vectors as carriers of designer nuclease proteins, providing efficient targeted gene disruption in vector-treated cell lines and primary cells. By co-packaging pairs of ZFN proteins with donor RNA in ‘all-in-one’ lentiviral particles, we co-deliver ZFN proteins and the donor template for homology-directed repair leading to targeted DNA insertion and gene correction. Comparative studies of ZFN activity in a predetermined target locus and a known nearby off-target locus demonstrate reduced off-target activity after ZFN protein transduction relative to conventional delivery approaches. Additionally, TALEN proteins are added to the repertoire of custom-designed nucleases that can be delivered by protein transduction. Altogether, our findings generate a new platform for genome engineering based on efficient and potentially safer delivery of programmable nucleases.

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