scholarly journals Replication Origin of Mitochondrial DNA in Insects

Genetics ◽  
2005 ◽  
Vol 171 (4) ◽  
pp. 1695-1705 ◽  
Author(s):  
Shigeru Saito ◽  
Koichiro Tamura ◽  
Tadashi Aotsuka
Keyword(s):  
Mitochondrial Dna ◽  
Replication Origin

scholarly journals Genetic analysis of systematic mitochondrial heteroplasmy in rabbits.

Genetics ◽  
1994 ◽  
Vol 138 (2) ◽  
pp. 471-480 ◽  
Author(s):  
D Casane ◽  
N Dennebouy ◽  
H de Rochambeau ◽  
J C Mounolou ◽  
M Monnerot
Keyword(s):  
Mitochondrial Dna ◽  
Replication Origin ◽  
Molecular Diversity ◽  
Germ Line ◽  
Variable Number ◽  
The Other ◽  
Molecular Forms ◽  
Unusual Property ◽  
Mitochondrial Heteroplasmy ◽  
Selection For

Abstract One unusual property of rabbit mitochondrial DNA (mtDNA) is the existence of repeated 153-bp motifs in the vicinity of the replication origin of its H strand. Furthermore, every individual is heteroplasmic: it carries mtDNA molecules with a variable number of repeats. A systematic study of 8 females and their progeny has been devised to analyze mtDNA transmission through generations. The results suggest that three mechanisms are acting simultaneously. (1) Genetic drift in the germ line is revealed by the evolution of heteroplasmy when two major molecular forms are present in a female. (2) A high mutation rate (around 10(-2) per animal generation) generating molecular diversity, by deletion and addition of repeated units, is required to explain the observation of heteroplasmy in every individual. Moreover, the rates of mutation from the most frequent type to the other types are unequal. The deletion of one unit is more frequent than a deletion of two units, which is in turn more frequent than a deletion of three. (3) Selection for shorter molecules in somatic cells is probable. The frequency distribution of mtDNA types depends on the organ analyzed (kidney-spleen and liver vs. gonads).

scholarly journals Sea urchin egg mitochondrial DNA contains a short displacement loop (D-loop) in the replication origin region

1989 ◽  
Vol 17 (22) ◽  
pp. 8949-8965 ◽  
Author(s):  
Howard T. Jacobs ◽  
Elaine R. Herbert ◽  
James Rankine
Keyword(s):  
Mitochondrial Dna ◽  
Sea Urchin ◽  
Replication Origin ◽  
Sea Urchin Egg ◽  
D Loop ◽  
Origin Region

Symmetric transcription of the replication origin of rat mitochondrial DNA

Gene ◽  
1988 ◽  
Vol 72 (1-2) ◽  
pp. 309-310 ◽  
Author(s):  
E. Sbisà ◽  
M. Nardelli ◽  
C. Saccone
Keyword(s):  
Mitochondrial Dna ◽  
Replication Origin

Nucleotide sequence of a cloned fragment of rat mitochondrial DNA containing the replication origin

Gene ◽  
1980 ◽  
Vol 11 (1-2) ◽  
pp. 53-62 ◽  
Author(s):  
Sekiya Takao ◽  
Kobayashi Midori ◽  
Seki Tetsunori ◽  
Koike Katsuro
Keyword(s):  
Mitochondrial Dna ◽  
Nucleotide Sequence ◽  
Replication Origin

scholarly journals DNA Recombination-Initiation Plays a Role in the Extremely Biased Inheritance of Yeast [rho−] Mitochondrial DNA That Contains the Replication Origin ori5

2006 ◽  
Vol 27 (3) ◽  
pp. 1133-1145 ◽  
Author(s):  
Feng Ling ◽  
Akiko Hori ◽  
Takehiko Shibata
Keyword(s):  
Mitochondrial Dna ◽  
Replication Origin ◽  
Excision Repair ◽  
Dna Recombination ◽  
Strand Break ◽  
Yeast Cells ◽  
Rolling Circle Replication ◽  
Rolling Circle ◽  
Mtdna Replication ◽  
Base Excision

ABSTRACT Hypersuppressiveness, as observed in Saccharomyces cerevisiae, is an extremely biased inheritance of a small mitochondrial DNA (mtDNA) fragment that contains a replication origin (HS [rho −] mtDNA). Our previous studies showed that concatemers (linear head-to-tail multimers) are obligatory intermediates for mtDNA partitioning and are primarily formed by rolling-circle replication mediated by Mhr1, a protein required for homologous mtDNA recombination. In this study, we found that Mhr1 is required for the hypersuppressiveness of HS [ori5] [rho −] mtDNA harboring ori5, one of the replication origins of normal ([rho +]) mtDNA. In addition, we detected an Ntg1-stimulated double-strand break at the ori5 locus. Purified Ntg1, a base excision repair enzyme, introduced a double-stranded break by itself into HS [ori5] [rho −] mtDNA at ori5 isolated from yeast cells. Both hypersuppressiveness and concatemer formation of HS [ori5] [rho −] mtDNA are simultaneously suppressed by the ntg1 null mutation. These results support a model in which, like homologous recombination, rolling-circle HS [ori5] [rho −] mtDNA replication is initiated by double-stranded breakage in ori5, followed by Mhr1-mediated homologous pairing of the processed nascent DNA ends with circular mtDNA. The hypersuppressiveness of HS [ori5] [rho −] mtDNA depends on a replication advantage furnished by the higher density of ori5 sequences and on a segregation advantage furnished by the higher genome copy number on transmitted concatemers.

scholarly journals Properties of some monkey DNA sequences obtained by a procedure that enriches for DNA replication origins.

1985 ◽  
Vol 5 (7) ◽  
pp. 1621-1629 ◽  
Author(s):  
M Zannis-Hadjopoulos ◽  
G Kaufmann ◽  
S S Wang ◽  
R L Lechner ◽  
E Karawya ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Dna Sequences ◽  
Replication Origin ◽  
Repetitive Sequences ◽  
S Phase ◽  
Alpha Satellite ◽  
Highly Dispersed ◽  
D Loop ◽  
Bona Fide ◽  
Dna Replication Origins

Twelve clones of monkey DNA obtained by a procedure that enriches 10(3)- to 10(4)-fold for nascent sequences activated early in S phase (G. Kaufmann, M. Zannis-Hadjopoulos, and R. G. Martin, Mol. Cell. Biol. 5:721-727, 1985) have been examined. Only 2 of the 12 ors sequences (origin-enriched sequences) are unique (ors1 and ors8). Three contain the highly reiterated Alu family (ors3, ors9, and ors11). One contains the highly reiterated alpha-satellite family (ors12), but none contain the Kpn family. Those remaining contain middle repetitive sequences. Two examples of the same middle repetitive sequence were found (ors2 and ors6). Three of the middle repetitive sequences (the ors2-ors6 pair, ors5, and ors10) are moderately dispersed; one (ors4) is highly dispersed. The last, ors7, has been mapped to the bona fide replication origin of the D loop of mitochondrial DNA. Of the nine ors sequences tested, half possess snapback (intrachain reannealing) properties.

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