Quality of Umbilical Cord Blood CD34 + Cells in a Double-Compartment Freezing Bag Cryopreserved without a Rate-Controlled Programmed Freezer

2007 ◽  
Vol 85 (1) ◽  
pp. 78-84 ◽  
Author(s):  
Masayoshi Minegishi ◽  
Tsuneo Itoh ◽  
Narumi Fukawa ◽  
Tamie Kitaura ◽  
Junko Miura ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Cd34 Cells ◽  
Cord Blood Cd34 ◽  
Rate Controlled ◽  
Double Compartment

scholarly journals High Integrity and Fidelity of Long-Term Cryopreserved Umbilical Cord Blood for Transplantation

2021 ◽  
Vol 10 (2) ◽  
pp. 293
Author(s):  
Gee-Hye Kim ◽  
Jihye Kwak ◽  
Sung Hee Kim ◽  
Hee Jung Kim ◽  
Hye Kyung Hong ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Clinical Applications ◽  
Hematopoietic Stem ◽  
Cd34 Cells ◽  
Hsc Transplantation

Umbilical cord blood (UCB) is used as a source of donor cells for hematopoietic stem cell (HSC) transplantation. The success of transplantation is dependent on the quality of cord blood (CB) units for maximizing the chance of engraftment. Improved outcomes following transplantation are associated with certain factors of cryopreserved CB units: total volume and total nucleated cell (TNC) count, mononuclear cell (MNC) count, and CD34+ cell count. The role of the storage period of CB units in determining the viability and counts of cells is less clear and is related to the quality of cryopreserved CB units. Herein, we demonstrate the recovery of viable TNCs and CD34+ cells, as well as the MNC viability in 20-year-old cryopreserved CB units in a CB bank (MEDIPOST Co., Ltd., Seongnam-si, Gyeonggi-do, Korea). In addition, cell populations in CB units were evaluated for future clinical applications. The stable recovery rate of the viability of cryopreserved CB that had been stored for up to 20 years suggested the possibility of uses of the long-term cryopreservation of CB units. Similar relationships were observed in the recovery of TNCs and CD34+ cells in units of cryopreserved and fresh CB. The high-viability recovery of long-term cryopreserved CB suggests that successful hematopoietic stem cell (HSC) transplantation and other clinical applications, which are suitable for treating incurable diseases, may be performed regardless of long-term storage.

In vitro clinical-grade generation of red blood cells from human umbilical cord blood CD34+ cells

Transfusion ◽  
2008 ◽  
Vol 48 (10) ◽  
pp. 2235-2245 ◽  
Author(s):  
Eun Jung Baek ◽  
Han-Soo Kim ◽  
Sinyoung Kim ◽  
Honglien Jin ◽  
Tae-Yeal Choi ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Blood Cells ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Clinical Grade ◽  
Cd34 Cells ◽  
Cord Blood Cd34

Short‐Term exposure of umbilical cord blood CD34 + cells to human platelet lysate and cytokines enhances engraftment

Transfusion ◽  
2020 ◽  
Vol 60 (10) ◽  
pp. 2348-2358
Author(s):  
Marie‐Ève Rhéaume ◽  
Pascal Rouleau ◽  
Tony Tremblay ◽  
Isabelle Paré ◽  
Lionel Loubaki
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Human Platelet ◽  
Platelet Lysate ◽  
Short Term ◽  
Short Term Exposure ◽  
Cd34 Cells ◽  
Cord Blood Cd34 ◽  
Human Platelet Lysate

Delta-4 Notch ligand promotes erythroid differentiation of human umbilical cord blood CD34+ cells

2006 ◽  
Vol 34 (4) ◽  
pp. 424-432 ◽  
Author(s):  
Akira Sugimoto ◽  
Mayuko Yamamoto ◽  
Motoyuki Suzuki ◽  
Toshiya Inoue ◽  
Shuji Nakamura ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Erythroid Differentiation ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Notch Ligand ◽  
Cd34 Cells ◽  
Cord Blood Cd34

Similar repopulating capacity of mitotically active and resting umbilical cord blood CD34+ cells in NOD/SCID mice

Blood ◽  
2000 ◽  
Vol 96 (6) ◽  
pp. 2100-2107 ◽  
Author(s):  
Jannine Wilpshaar ◽  
J. H. Frederik Falkenburg ◽  
Xia Tong ◽  
Willy A. Noort ◽  
Robert Breese ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Linear Models ◽  
Human Cells ◽  
Scid Mice ◽  
Mammalian Development ◽  
Hematopoietic Stem ◽  
Cd34 Cells ◽  
Cord Blood Cd34

It was hypothesized that during mammalian development, the extensive need for hematopoietic cells requires equal contribution to blood cell production from both quiescent and cycling hematopoietic stem cells (HSCs) while maintaining the stem cell pool. To investigate this hypothesis, the engraftment potential of umbilical cord blood (UCB) CD34+ cells residing in either G0(G0CD34+ cells) or G1(G1CD34+ cells) phases of the cell cycle was assessed in nonobese diabetic/severe combined immune-deficient (NOD/SCID) mice. Whereas the level of chimerism in mice transplanted with UCB G0CD34+ cells was 69.9% ± 24.0%, mice receiving equal numbers of G1CD34+ cells harbored 46.7% ± 21.3% human cells 8 weeks posttransplantation. Both groups of cells sustained multilineage differentiation and the production of CD34+cells in recipient animals. The relationship between the number of transplanted G0CD34+ or G1CD34+ cells and the level of chimerism was analyzed by a general linear models procedure. Although the initial level of chimerism following transplantation of G0CD34+ cells was higher than that sustained by G1CD34+ cells, the increment in the degree of chimerism obtained with each additional 103 cells of either phenotype was identical, suggesting that the reconstitution potential of these 2 types of cells was similar. Of interest is that human cells recovered from primary recipients of both G0CD34+ and G1CD34+cells engrafted in secondary NOD/SCID recipients, albeit at a substantially lower level, confirming the primitive nature of UCB CD34+ cells residing in G1.

Effects of intravenous administration of umbilical cord blood CD34+ cells in a mouse model of neonatal stroke

Neuroscience ◽  
2014 ◽  
Vol 263 ◽  
pp. 148-158 ◽  
Author(s):  
M. Tsuji ◽  
A. Taguchi ◽  
M. Ohshima ◽  
Y. Kasahara ◽  
Y. Sato ◽  
...  
Keyword(s):  
Mouse Model ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Intravenous Administration ◽  
Umbilical Cord Blood ◽  
Neonatal Stroke ◽  
Cd34 Cells ◽  
Cord Blood Cd34
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