Preantral follicle development in cultured reaggregated neonatal ovaries

2016 ◽  
Author(s):  
Belinda Lo ◽  
Sairah Sheikh ◽  
Suzannah Williams
Keyword(s):  
Follicle Development ◽  
Preantral Follicle

Effects of follicular fluids on the growth of porcine preantral follicle and oocyte

Zygote ◽  
2008 ◽  
Vol 16 (3) ◽  
pp. 239-247 ◽  
Author(s):  
T. Metoki ◽  
H. Iwata ◽  
M. Itoh ◽  
M. Kasai ◽  
A. Takajyo ◽  
...  
Keyword(s):  
Calf Serum ◽  
Follicle Development ◽  
Preantral Follicle ◽  
Promoting Effect ◽  
Oocyte Growth ◽  
Preantral Follicles ◽  
Molecular Weights ◽  
Heat Treated ◽  
Growth Promoting ◽  
Follicular Fluids

SummaryWe examined the effect of supplementing the culture medium with follicular fluid (FF) on the growth of porcine preantral follicles and oocytes. Firstly, preantral follicles were retrieved from ovaries and then FF was collected from all antral follicles that were 2–7 mm in diameter (AFF), which included large follicles of 4–7 mm in diameter (LFF) and small follicles of 2–3 mm in diameter (SFF). When preantral follicles with a diameter of 250 μm were cultured in medium containing AFF, the growth of follicles and oocytes was greater than when follicles were cultured in medium containing fetal calf serum (FCS). When this growth-promoting effect in AFF was compared for LFF and SFF, the LFF were shown to be significantly more effective than SFF. This LFF effect was lost, however, when the concentration of LFF in the medium was decreased from 5% to 0.5% or when LFF were heat treated (60 °C for 30 min) or trypsin was added. In contrast, a decrease in SFF concentration from 5% to 0.5% and heat treatment of the SFF enhanced preantral follicle growth. Furthermore, proteins obtained from LFF that had molecular weights greater than 10 kDa (LFF > 10 kDa) had similar, but relatively reduced, growth-promoting properties. The remaining three LFF protein fractions (<10 kDa or <100 kDa or >100 kDa), however, did not have these growth-promoting properties. In conclusion, the supplementation of medium with LFF, rather than serum, enhanced preantral follicle and oocyte growth. Factors that enhanced follicle development in LFF and factors that suppressed follicle development in SFF were proteins and these LFF factors ranged in size from 10 kDa to over 100 kDa.

Impact of growth hormone (GH) and follicle stimulating hormone (FSH) on in vitro canine preantral follicle development and estradiol production

2015 ◽  
Vol 25 (2) ◽  
pp. 85-89 ◽  
Author(s):  
M.K.B. Serafim ◽  
A.B.G. Duarte ◽  
G.M. Silva ◽  
C.E.A. Souza ◽  
D.M. Magalhães-Padilha ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Follicle Stimulating Hormone ◽  
Follicle Development ◽  
Preantral Follicle ◽  
Stimulating Hormone

Regulators of Ovarian Preantral Follicle Development

2015 ◽  
Vol 33 (03) ◽  
pp. 179-184 ◽  
Author(s):  
Renju Raj ◽  
Elizabeth McGee
Keyword(s):  
Follicle Development ◽  
Preantral Follicle

scholarly journals Luteinizing Hormone Has a Stage-Limited Effect on Preantral Follicle Development In Vitro1

2000 ◽  
Vol 63 (1) ◽  
pp. 320-327 ◽  
Author(s):  
Ji Wu ◽  
Penelope L. Nayudu ◽  
Petra S. Kiesel ◽  
Hans W. Michelmann
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Development ◽  
Preantral Follicle ◽  
Limited Effect

scholarly journals Insulin-like growth factor 2 is produced by antral follicles and promotes preantral follicle development in macaques

2020 ◽  
Author(s):  
Olena Y Tkachenko ◽  
Shally Wolf ◽  
Maralee S Lawson ◽  
Alison Y Ting ◽  
Jhenifer K Rodrigues ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Granulosa Cell ◽  
Steroid Hormone ◽  
Follicle Development ◽  
Hormone Regulation ◽  
Preantral Follicle ◽  
Paracrine Factor ◽  
Preantral Follicles ◽  
Antral Follicles ◽  
And Function

Abstract Insulin-like growth factors (IGFs) are known for their involvement in endocrine and paracrine regulation of ovarian function. While IGF2 is the predominant circulating and intra-ovarian form of IGFs in primate species, the stage-specific follicular expression, action and regulation of IGF2 are not well defined. Therefore, experiments were conducted to investigate the follicular IGF production in response to steroid hormone regulation and the direct IGF actions on follicular development and function in vitro. Preantral follicles were isolated from rhesus macaque ovaries and cultured to the antral stage in media supplemented with follicle-stimulating hormone and insulin. Follicles were randomly assigned to treatment groups: (a) control, (b) trilostane (a steroid synthesis inhibitor), (c) trilostane + estradiol, (d) trilostane + progesterone, and (e) trilostane + dihydrotestosterone. Media was analyzed for IGF concentrations, which were correlated to follicle growth. Follicles produced IGF2, but not IGF1, at the antral stage. Steroid depletion decreased, while steroid replacement increased, IGF2 production by antral follicles. Media IGF2 levels correlated positively with antral follicle diameters. Macaque preantral follicles and granulosa cells were subsequently cultured without (control) and with recombinant human IGF2 supplementation. Follicle survival, growth and paracrine factor production, as well as granulosa cell proliferation and gonadotropin receptor gene expression, were assessed. IGF2 addition increased follicle survival rates, diameters and inhibin B production, as well as granulosa cell proliferation. These data demonstrate that IGF2 produced by antral follicles, in response to steroid hormone regulation, could act as a paracrine factor that positively impacts preantral follicle development and function in primates.

123. ACTIVIN A AND OVARIAN FOLLICLE DEVELOPMENT

2009 ◽  
Vol 21 (9) ◽  
pp. 42
Author(s):  
D. A. Cossigny (Rosairo) ◽  
J. K. Findlay ◽  
A. E. Drummond
Keyword(s):  
Ovarian Follicle ◽  
Combined Treatment ◽  
Rna Extraction ◽  
Activin A ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Follicle Development ◽  
Preantral Follicle ◽  
Primary Follicle ◽  
Preantral Follicles ◽  
Ovarian Follicle Development

A significant developmental stage in ovarian folliculogenesis is the acquisition of gonadotropin sensitivity by ovarian follicles. Activin has previously been suggested to be involved in the responsiveness of granulosa cells to FSH (1). Therefore, the role of activin was investigated using a ‘physiological’ culture system to determine if pathways exist to transduce activin signals within the postnatal rat ovary. Organ cultures with day 4 whole ovaries were employed in order to assess the potential impact of Activin A on follicle growth and transition from the primordial through to the primary and later preantral stages of development. Ovaries were isolated and cultured for 10 days with the addition of supplemented DMEM/Hams F-12 media (2)and either FSH (100ng/ml), Activin A (50ng/ml), or a combination of the two. Media and treatments were refreshed every alternate day. At the end of the culture period, ovaries were fixed and sectioned, or placed immediately into Ultraspec for RNA extraction for future real-time PCR. Sections were used for morphological assessment and ovarian follicle counting of primordial, primary and preantral follicles. An evaluation of atresia by the detection of apoptotic cells was undertaken using terminal deoxynucleotidyl transferase (TdT) mediated dUTP-biotin nick-end labeling (TUNEL). Primary follicle numbers increased significantly (P<0.05) in the combined treatment group whereas, preantral follicle numbers increased significantly (P<0.0001) when treated with Activin A alone. This is consistent with a morphological appraisal of atresia where a decrease in atresia was found in primordial and primary follicles, supporting the primary follicle development data and Activin A treatment alone resulted in more healthy primary and preantral follicles than atretic ones. Therefore, a stimulatory role for Activin A both in the presence of FSH (primary follicle development) or alone (preantral follicle development) has resulted in more follicles making the transition from the primordial to primary stages, as well as to the later preantral stages.

Influence of thyroid hormone on mouse preantral follicle development in vitro

2004 ◽  
Vol 81 ◽  
pp. 919-924 ◽  
Author(s):  
Sandra Cecconi ◽  
Gianna Rossi ◽  
Giovanni Coticchio ◽  
Guido Macchiarelli ◽  
Andrea Borini ◽  
...  
Keyword(s):  
Thyroid Hormone ◽  
Follicle Development ◽  
Preantral Follicle ◽  
Development In Vitro
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