Caveolins redistribute in uterine epithelial cells during early pregnancy: role in epithelial polarisation

2014 ◽  
Author(s):  
Romanthi J Madawala ◽  
Samson Dowland ◽  
Connie E Poon ◽  
Christopher Murphy
Keyword(s):  
Epithelial Cells ◽  
Early Pregnancy ◽  
Uterine Epithelial Cells

310. CAVEOLIN 1 AND 2 IN THE UTERUS DURING EARLY PREGNANCY

2010 ◽  
Vol 22 (9) ◽  
pp. 110
Author(s):  
R. J. Madawala ◽  
C. R. Murphy
Keyword(s):  
Plasma Membrane ◽  
Epithelial Cells ◽  
Protein Expression ◽  
Early Pregnancy ◽  
Basolateral Membrane ◽  
Membrane Curvature ◽  
Major Protein ◽  
Uterine Epithelial Cells ◽  
Caveolin 1 ◽  
Blastocyst Implantation

Rat uterine epithelial cells undergo many changes during early pregnancy in order to become receptive to blastocyst implantation. These changes include basolateral folding and the presence of vesicles of various sizes which are at their greatest number during the pre-implantation period. The present study investigated the possible role that caveolin 1 and 2 plays in this remodelling specifically days 1, 3, 6, 7, and 9 of pregnancy. Caveolin is a major protein in omega shaped invaginations of the plasma membrane called caveolae that are considered to be specialised plasma membrane subdomains. Caveolae are rich in cholesterol, glycosphingolipids, and GPI anchored proteins and are involved in endocytosis and membrane curvature. Immunofluorescence microscopy has shown caveolin 1 and 2 on day 1 of pregnancy are localised to the cytoplasm of luminal uterine epithelial cells, and by day 6 of pregnancy (the time of implantation), it concentrates basally. By day 9 of pregnancy, expression of both caveolin 1 and 2 in luminal uterine epithelia is cytoplasmic as seen on day 1 of pregnancy. A corresponding increase in protein expression of caveolin 1 on day 6 of pregnancy in luminal uterine epithelia was observed. Interestingly however, caveolin 2 protein expression decreases at the time of implantation as found by western blot analysis. Both caveolin 1 and 2 were localised to blood vessels within the endometrium and myometrium and also the muscle of the myometrium in all days of pregnancy studied. In addition, both caveolin 1 and 2 were absent from glandular epithelium, which is interesting considering that they do not undergo the plasma membrane transformation. The localisation and expression of caveolin 1 and 2 in rat luminal uterine epithelium at the time of implantation suggest possible roles in trafficking of cholesterol and/or various proteins for either degradation or relocation. Caveolins may contribute to the morphology of the basolateral membrane seen on day 6 of pregnancy. All of which may play an important role during successful blastocyst implantation.

445. Focal adhesions disassemble during early pregnancy in rat uterine epithelial cells

2008 ◽  
Vol 20 (9) ◽  
pp. 125
Author(s):  
Y. Kaneko ◽  
L. A. Lindsay ◽  
C. R. Murphy
Keyword(s):  
Epithelial Cells ◽  
Western Blotting ◽  
Early Pregnancy ◽  
Active Form ◽  
Focal Adhesions ◽  
Proteolytic Fragment ◽  
Uterine Epithelial Cells ◽  
Western Blotting Analysis ◽  
Blotting Analysis ◽  
Calpain 2

Successful blastocyst implantation require uterine epithelial cells (UECs) to undergo the 'plasma membrane transformation' followed by the removal of these cells around the implantation sites. The present study investigated the distribution and expression of two principal focal adhesion proteins talin and paxillin in rat UECs during early pregnancy and their role in the loss of these cells at the time of implantation. Results from immunofluorescence microscopy have demonstrated a major distributional change of talin and paxillin in UECs where an intense basal staining of these proteins on day 1 of pregnancy was lost at time of implantation. This was consistent with the significant decrease in paxillin seen through western blotting analysis. Interestingly the amount of talin was not significantly different between day 1 of pregnancy and at the time of implantation with a calpain 2 mediated proteolytic fragment of talin seen on both of these days of pregnancy. Calpain 2 activity was further investigated through western blotting analysis and increases in the active form of calpain 2 at the time of implantation. These observations suggest that talin and paxillin have disassembled from the site of focal adhesions where talin is undergoing cleavage by active calpain 2 at the time of implantation. This allows UECs to become less adherent to the underlying basal lamina facilitating their removal during blastocyst invasion. Hence, disassembly of focal adhesions at the time of implantation is a critical event for successful implantation and placentation.

Claudin 7 is reduced in uterine epithelial cells during early pregnancy in the rat

2012 ◽  
Vol 139 (4) ◽  
pp. 583-593 ◽  
Author(s):  
Connie E. Poon ◽  
Romanthi J. Madawala ◽  
Margot L. Day ◽  
Christopher R. Murphy
Keyword(s):  
Epithelial Cells ◽  
Early Pregnancy ◽  
Uterine Epithelial Cells

Caveolins redistribute in uterine epithelial cells during early pregnancy in the rat: An epithelial polarisation strategy?

2014 ◽  
Vol 142 (5) ◽  
pp. 555-567 ◽  
Author(s):  
Romanthi J. Madawala ◽  
Sam Dowland ◽  
Connie E. Poon ◽  
Laura A. Lindsay ◽  
Christopher R. Murphy
Keyword(s):  
Epithelial Cells ◽  
Early Pregnancy ◽  
Uterine Epithelial Cells
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