Panel of MABs to sperm surface proteins as tool for monitoring of sperm-zona pellucida receptors localization and identification

2014 ◽  
Author(s):  
Michal Zigo ◽  
Andriy Dorosh ◽  
Alzbeta Pohlova ◽  
Vera Jonakova ◽  
Miroslav Sulc ◽  
...  
Keyword(s):  
Zona Pellucida ◽  
Surface Proteins ◽  
Sperm Surface ◽  
Sperm Surface Proteins

Panel of monoclonal antibodies to sperm surface proteins as a tool for monitoring localization and identification of sperm–zona pellucida receptors

2014 ◽  
Vol 359 (3) ◽  
pp. 895-908 ◽  
Author(s):  
Michal Zigo ◽  
Andriy Dorosh ◽  
Alžběta Pohlová ◽  
Věra Jonáková ◽  
Miroslav Šulc ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Zona Pellucida ◽  
Surface Proteins ◽  
Sperm Surface ◽  
Sperm Surface Proteins

IDENTIFICATION OF BOAR SPERM SURFACE PROTEINS WITH AFFINITY FOR PORCINE ZONA PELLUCIDA

1995 ◽  
Vol 31 (1) ◽  
pp. 229-231
Author(s):  
M. Enßlin ◽  
J.J. Calvete ◽  
H.H. Thole ◽  
W.D. Sierralta ◽  
E. Töpfer-Petersen
Keyword(s):  
Zona Pellucida ◽  
Surface Proteins ◽  
Sperm Surface ◽  
Boar Sperm ◽  
Sperm Surface Proteins

scholarly journals Buffalo sperm surface proteome profiling reveals an intricate relationship between innate immunity and reproduction

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Vipul Batra ◽  
Vanya Bhushan ◽  
Syed Azmal Ali ◽  
Parul Sarwalia ◽  
Ankit Pal ◽  
...  
Keyword(s):  
Male Fertility ◽  
Female Reproductive Tract ◽  
Surface Proteins ◽  
Glycosylation Pattern ◽  
Sperm Surface ◽  
Beta Defensins ◽  
Surface Proteome ◽  
Related Proteins ◽  
Sperm Surface Proteins

Abstract Background Low conception rate (CR) despite insemination with morphologically normal spermatozoa is a common reproductive restraint that limits buffalo productivity. This accounts for a significant loss to the farmers and the dairy industry, especially in agriculture-based economies. The immune-related proteins on the sperm surface are known to regulate fertility by assisting the spermatozoa in their survival and performance in the female reproductive tract (FRT). Regardless of their importance, very few studies have specifically catalogued the buffalo sperm surface proteome. The study was designed to determine the identity of sperm surface proteins and to ascertain if the epididymal expressed beta-defensins (BDs), implicated in male fertility, are translated and applied onto buffalo sperm surface along with other immune-related proteins. Results The raw mass spectra data searched against an in-house generated proteome database from UniProt using Comet search engine identified more than 300 proteins on the ejaculated buffalo sperm surface which were bound either by non-covalent (ionic) interactions or by a glycosylphosphatidylinositol (GPI) anchor. The singular enrichment analysis (SEA) revealed that most of these proteins were extracellular with varied binding activities and were involved in either immune or reproductive processes. Flow cytometry using six FITC-labelled lectins confirmed the prediction of glycosylation of these proteins. Several beta-defensins (BDs), the anti-microbial peptides including the BuBD-129 and 126 were also identified amongst other buffalo sperm surface proteins. The presence of these proteins was subsequently confirmed by RT-qPCR, immunofluorescence and in vitro fertilization (IVF) experiments. Conclusions The surface of the buffalo spermatozoa is heavily glycosylated because of the epididymal secreted (glyco) proteins like BDs and the GPI-anchored proteins (GPI-APs). The glycosylation pattern of buffalo sperm-surface, however, could be perturbed in the presence of elevated salt concentration or incubation with PI-PLC. The identification of numerous BDs on the sperm surface strengthens our hypothesis that the buffalo BDs (BuBDs) assist the spermatozoa either in their survival or in performance in the FRT. Our results suggest that BuBD-129 is a sperm-surface BD that could have a role in buffalo sperm function. Further studies elucidating its exact physiological function are required to better understand its role in the regulation of male fertility.

scholarly journals Buffalo Sperm Surface Proteome Profiling Reveals an Intricate Relationship Between Innate Immunity and Reproduction

2021 ◽  
Author(s):  
Vipul Batra ◽  
Vanya Bhushan ◽  
Syed Ali ◽  
Parul Sarwalia ◽  
Subhash Solanki ◽  
...  
Keyword(s):  
Male Fertility ◽  
Significant Loss ◽  
Female Reproductive Tract ◽  
Surface Proteins ◽  
Glycosylation Pattern ◽  
Sperm Surface ◽  
Beta Defensins ◽  
Surface Proteome ◽  
Related Proteins ◽  
Sperm Surface Proteins

Abstract BackgroundLow conception rate (CR) despite insemination with morphologically normal spermatozoa is a common reproductive restraint which limits buffalo productivity. This accounts for a significant loss to the farmers and the dairy industry, especially in agriculture-based economies. The immune-related proteins on the sperm surface are known to regulate fertility by assisting the spermatozoa in their survival and performance in the female reproductive tract (FRT). Regardless of their importance, very few studies have specifically catalogued the buffalo sperm surface proteome. The study was designed to determine the identity of sperm surface proteins and to ascertain if the epididymal expressed beta-defensins (BDs), implicated in male fertility, are translated and applied onto buffalo sperm surface along with other immune-related proteins. ResultsThe raw mass spectra data searched against in-house generated proteome database from UniProt using Comet search engine identified more than 300 proteins on the ejaculated buffalo sperm surface which were bound either by non-covalent (ionic) interactions or by a GPI-anchor. The singular enrichment analysis (SEA) revealed that most of these proteins were extracellular with varied binding activities and were involved in either immune or reproductive processes. Flow cytometry using six FITC-labelled lectins confirmed the prediction of glycosylation of these proteins. Several beta-defensins (BDs), the anti-microbial peptides including the BuBD-129 and 126 were also identified amongst the buffalo sperm surface proteins. The presence of these proteins was confirmed by immunocytochemistry and IVF experiments. Conclusions The surface of the buffalo spermatozoa is heavily glycosylated because of the epididymal secreted glycoproteins like BDs and the GPI-anchored proteins. The glycosylation pattern, however, could be perturbed in the presence of elevated salt concentration or incubation with PI-PLC. The identification of numerous BuBDs on the sperm surface strengthens our hypothesis that these BDs assist the buffalo spermatozoa either in their survival or in performance in the FRT. Our results suggest that BuBD-129 is a sperm-surface BD which could have a role in buffalo sperm function. Further studies elucidating its exact physiological function are required to better understand its roles in the regulation of male fertility.

Relation between tyrosine phosphorylation of the equine sperm surface proteins and acrosome reaction

2008 ◽  
Vol 107 (3-4) ◽  
pp. 304-305 ◽  
Author(s):  
A.F.C. Andrade ◽  
F.G. Zaffalon ◽  
G.K.F. Merighe ◽  
J. Nascimento ◽  
O.F.B. Tarragó ◽  
...  
Keyword(s):  
Tyrosine Phosphorylation ◽  
Acrosome Reaction ◽  
Surface Proteins ◽  
Sperm Surface ◽  
Sperm Surface Proteins
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